Guifeng Wei
@guifengwei.bsky.social
RNA modifications, X-inactivation, epigenetics, bioinformatics
https://sites.google.com/view/guifengwei/
https://sites.google.com/view/guifengwei/
brilliant
New public hub: Sci-ModoM RNA modification sites. 7.9M m6A, pseudouridine, m5C and 2'-O-methylation sites on hg38 and mm39, each with its coverage and percent modified.
Explore hub: genome.ucsc.edu/s/Lo...
More public hubs: genome.ucsc.edu/cgi-...
Explore hub: genome.ucsc.edu/s/Lo...
More public hubs: genome.ucsc.edu/cgi-...
September 21, 2026 at 8:10 PM
brilliant
Reposted by Guifeng Wei
Excited to share our new preprint on in situ chromatin structure of the inactive X chromosome in differentiated female mouse embryonic stem cells.
www.biorxiv.org/content/10.6...
🧵1/8
www.biorxiv.org/content/10.6...
🧵1/8
September 14, 2026 at 8:35 AM
Excited to share our new preprint on in situ chromatin structure of the inactive X chromosome in differentiated female mouse embryonic stem cells.
www.biorxiv.org/content/10.6...
🧵1/8
www.biorxiv.org/content/10.6...
🧵1/8
Reposted by Guifeng Wei
Very happy to share the first pre-print from my postdoc work with @larsplus.bsky.social (which we also presented at the excellent @isehsociety.bsky.social Annual Meeting last weekend). Here we performed a CRISPRi Perturb-seq screen targeting >500 transcriptional regulators in ex vivo mouse HSPCs.
September 1, 2026 at 2:07 PM
Very happy to share the first pre-print from my postdoc work with @larsplus.bsky.social (which we also presented at the excellent @isehsociety.bsky.social Annual Meeting last weekend). Here we performed a CRISPRi Perturb-seq screen targeting >500 transcriptional regulators in ex vivo mouse HSPCs.
Reposted by Guifeng Wei
Today, I would like to honor the memory of Roger Y. Tsien, who died on August 24, 2016. His legacy lives with all who use his technologies, including calcium sensors, fluorescent proteins, the acetoxymethyl (AM) ester, & many more! #FluorescenceFriday
www.nature.com/articles/nme...
www.nature.com/articles/nme...
August 21, 2026 at 1:11 PM
Today, I would like to honor the memory of Roger Y. Tsien, who died on August 24, 2016. His legacy lives with all who use his technologies, including calcium sensors, fluorescent proteins, the acetoxymethyl (AM) ester, & many more! #FluorescenceFriday
www.nature.com/articles/nme...
www.nature.com/articles/nme...
An elegant explanation for why L1 is enriched on the X chromosome!
Our study published today @science.org shows that X-chromosome inactivation (XCI) attracts L1 mutations to the human X chromosome:
doi.org/10.1126/scie...
With outstanding Perspective from @aurelien-doucet.bsky.social & @retrogenomics.bsky.social:
doi.org/10.1126/scie...
doi.org/10.1126/scie...
With outstanding Perspective from @aurelien-doucet.bsky.social & @retrogenomics.bsky.social:
doi.org/10.1126/scie...
July 31, 2026 at 11:18 AM
An elegant explanation for why L1 is enriched on the X chromosome!
Super-exciting live-imaging method of nuclear RNA!
Amazing new method for live-cell imaging of RNA molecules developed by Adam in the Brockdorff lab! Worth having a look at!
Nuclear-BRITE: an enhanced MS2 system for live-cell imaging of nuclear RNAs https://www.biorxiv.org/content/10.64898/2026.07.07.736975v1
July 10, 2026 at 6:47 AM
Super-exciting live-imaging method of nuclear RNA!
Reposted by Guifeng Wei
New preprint 📢 Thrilled to share our long-awaited work on the role of RSX in marsupial X-inactivation. This was a huge team effort over the years, led by @aureliencourtois.bsky.social @sermenchero.bsky.social Sugako Ogushi and Waz Varsally, that allowed us to set up genome editing in opossum embryos
Genome editing of marsupial RSX reveals conserved and divergent principles in mammalian X-inactivation
X-chromosome inactivation balances X-gene dosage between the sexes in eutherians and marsupials. The lncRNA Xist mediates X-inactivation in eutherians but is absent from marsupials. An evolutionarily ...
www.biorxiv.org
July 8, 2026 at 9:50 AM
New preprint 📢 Thrilled to share our long-awaited work on the role of RSX in marsupial X-inactivation. This was a huge team effort over the years, led by @aureliencourtois.bsky.social @sermenchero.bsky.social Sugako Ogushi and Waz Varsally, that allowed us to set up genome editing in opossum embryos
Reposted by Guifeng Wei
🚨🚨NEW PREPRINT ALERT!🚨🚨Proud to present our recent study, out now on bioRxiv, in which we reveal an RNA-dependent positive feedback loop that drives malignant ribosome biogenesis and tumour growth (1/15).
🔗
www.biorxiv.org/content/10.6...
🔗
www.biorxiv.org/content/10.6...
July 3, 2026 at 7:18 PM
🚨🚨NEW PREPRINT ALERT!🚨🚨Proud to present our recent study, out now on bioRxiv, in which we reveal an RNA-dependent positive feedback loop that drives malignant ribosome biogenesis and tumour growth (1/15).
🔗
www.biorxiv.org/content/10.6...
🔗
www.biorxiv.org/content/10.6...
Reposted by Guifeng Wei
There is more to transcriptional readthrough than meets the eye, and it could even provide a therapeutic opportunity in cancer. Check out our new perspective, inspired by recent work from Neugebauer and Grzechnik labs! #RNABiology #RNASky #Epigenetics
Studying gene ends is becoming even more fascinating! Curious why? Read our News & Views "Transcriptional readthrough progresses from incidental byproduct to therapeutic opportunity" inspired by the recent publication by Podszywałow-Bartnicka and @karlaneugebauer.bsky.social @natsmb.nature.com
July 1, 2026 at 12:07 PM
There is more to transcriptional readthrough than meets the eye, and it could even provide a therapeutic opportunity in cancer. Check out our new perspective, inspired by recent work from Neugebauer and Grzechnik labs! #RNABiology #RNASky #Epigenetics
Reposted by Guifeng Wei
🔥 Published today, and just in time for summer conferences, here are your guidelines for presenting badly
www.nature.com/articles/s41... rdcu.be/fqa92
www.nature.com/articles/s41... rdcu.be/fqa92
June 24, 2026 at 4:44 PM
🔥 Published today, and just in time for summer conferences, here are your guidelines for presenting badly
www.nature.com/articles/s41... rdcu.be/fqa92
www.nature.com/articles/s41... rdcu.be/fqa92
Reposted by Guifeng Wei
I only want to strongly emphasize that everyone must go to the @qedscience.bsky.social homepage to see their AI slop promotional ads. They are DERANGED! Two end in scientists dying, and one strongly implies bestiality. I can't get them out of my head!
🎉 Meet The 1%.
Out of 57,455 bioRxiv life science preprints, QED Science selected 574 exceptional manuscripts through blind evaluation.
The 1%: the-one-percent.qedscience.com
Good science deserves to be seen.
@biorxivpreprint.bsky.social @openrxiv.bsky.social @richardsever.bsky.social
Out of 57,455 bioRxiv life science preprints, QED Science selected 574 exceptional manuscripts through blind evaluation.
The 1%: the-one-percent.qedscience.com
Good science deserves to be seen.
@biorxivpreprint.bsky.social @openrxiv.bsky.social @richardsever.bsky.social
June 24, 2026 at 3:37 PM
I only want to strongly emphasize that everyone must go to the @qedscience.bsky.social homepage to see their AI slop promotional ads. They are DERANGED! Two end in scientists dying, and one strongly implies bestiality. I can't get them out of my head!
This study led by @flavia-con.bsky.social from the Brockdorff lab is finally published at Nature Communications!
www.nature.com/articles/s41...
Using live-cell and single-molecule imaging, this work dissects how the epigenetic regulator #SMCHD1 interacts with chromatin during X-inactivation.
www.nature.com/articles/s41...
Using live-cell and single-molecule imaging, this work dissects how the epigenetic regulator #SMCHD1 interacts with chromatin during X-inactivation.
June 16, 2026 at 9:42 PM
This study led by @flavia-con.bsky.social from the Brockdorff lab is finally published at Nature Communications!
www.nature.com/articles/s41...
Using live-cell and single-molecule imaging, this work dissects how the epigenetic regulator #SMCHD1 interacts with chromatin during X-inactivation.
www.nature.com/articles/s41...
Using live-cell and single-molecule imaging, this work dissects how the epigenetic regulator #SMCHD1 interacts with chromatin during X-inactivation.
Reposted by Guifeng Wei
New paper! How do RNAs "know" where to go inside a cell? We dug into the sequence elements that route RNAs to the right place. It turns out that, in mammals, they're surprisingly massive (>200 nt), multipartite, and wonderfully complicated. 🧵
June 11, 2026 at 2:59 PM
New paper! How do RNAs "know" where to go inside a cell? We dug into the sequence elements that route RNAs to the right place. It turns out that, in mammals, they're surprisingly massive (>200 nt), multipartite, and wonderfully complicated. 🧵
Reposted by Guifeng Wei
Postdoc position available now in the Smith Group lsi.exeter.ac.uk/groups/smith... to test the hypothesis that differentiation biases between human pluripotent stem cell lines may be eliminated by reversion to the naïve state.
careers.exeter.ac.uk/vacancies/79...
careers.exeter.ac.uk/vacancies/79...
Smith Group - Living Systems Institute - University of Exeter
Understanding pluripotency
lsi.exeter.ac.uk
June 9, 2026 at 10:09 AM
Postdoc position available now in the Smith Group lsi.exeter.ac.uk/groups/smith... to test the hypothesis that differentiation biases between human pluripotent stem cell lines may be eliminated by reversion to the naïve state.
careers.exeter.ac.uk/vacancies/79...
careers.exeter.ac.uk/vacancies/79...
Reposted by Guifeng Wei
🧵 Happy to share that the final version of our study is finally out in @natcomms.nature.com today:
www.nature.com/articles/s41...
What started as an unexpected observation—that SETDB1 depletion accelerates X chromosome silencing—led us to uncover a role for SETDB1 in regulating Xist transcription.
www.nature.com/articles/s41...
What started as an unexpected observation—that SETDB1 depletion accelerates X chromosome silencing—led us to uncover a role for SETDB1 in regulating Xist transcription.
SETDB1 and HUSH modulate Xist RNA levels during establishment of X chromosome inactivation - Nature Communications
Regulation of X chromosome inactivation in mammals requires precise control of the levels of the non-coding RNA Xist. Almeida et al. describe an autoregulatory mechanism in which nascent Xist transcri...
www.nature.com
June 8, 2026 at 8:54 PM
🧵 Happy to share that the final version of our study is finally out in @natcomms.nature.com today:
www.nature.com/articles/s41...
What started as an unexpected observation—that SETDB1 depletion accelerates X chromosome silencing—led us to uncover a role for SETDB1 in regulating Xist transcription.
www.nature.com/articles/s41...
What started as an unexpected observation—that SETDB1 depletion accelerates X chromosome silencing—led us to uncover a role for SETDB1 in regulating Xist transcription.
Reposted by Guifeng Wei
Today we celebrate our colleagues Colin Kleanthous and Rob Klose as they join the Fellowship of the Royal Society, one of the highest honours in the scientific world, in recognition of their impactful contributions to knowledge. Congratulations! 👏 👏 👏
Read more ⬇️
Read more ⬇️
Congratulations to Colin Kleanthous and Rob Klose on joining the Fellowship of the Royal Society
The two scientists have been selected for outstanding contributions to their fields
bit.ly
May 27, 2026 at 10:18 AM
Today we celebrate our colleagues Colin Kleanthous and Rob Klose as they join the Fellowship of the Royal Society, one of the highest honours in the scientific world, in recognition of their impactful contributions to knowledge. Congratulations! 👏 👏 👏
Read more ⬇️
Read more ⬇️
Reposted by Guifeng Wei
New online! The discovery of gene imprinting
The discovery of gene imprinting
Nature Reviews Genetics, Published online: 19 May 2026; doi:10.1038/s41576-026-00973-zIn this Journal Club, Mary Gehring recalls a 1970 paper from Jerry Kermicle, which used elegant maize genetics to show that alleles behave differently depending on parental origin, laying the conceptual foundation for gene imprinting.
dlvr.it
May 19, 2026 at 3:04 PM
New online! The discovery of gene imprinting
Reposted by Guifeng Wei
New online! RNA design across eras: from covariance models to modern generative AI
RNA design across eras: from covariance models to modern generative AI
Nature Reviews Genetics, Published online: 08 May 2026; doi:10.1038/s41576-026-00967-xIn this Journal Club, Annalisa Marsico describes a 1994 paper by Eddy and Durbin that formalized RNA sequence analysis with covariance models, which are powerful tools more recently applied to generative machine learning models for RNA design.
dlvr.it
May 9, 2026 at 5:56 AM
New online! RNA design across eras: from covariance models to modern generative AI
Reposted by Guifeng Wei
Two new papers 📖 from our lab 1️⃣ What is epigenetics (and why X inactivation is?) rdcu.be/fhzSz and 2️⃣ A Baculovirus-based tool to express any gene of interest with high efficiency in Anopheles cells rdcu.be/fhzTy
The epigenetic nature of X chromosome inactivation
Nature Reviews Genetics - In this Journal Club, Claudia Keller Valsecchi recalls two seminal studies by Csankovszki et al. and by Wutz and Jaenisch that defined X-chromosome inactivation as an...
rdcu.be
May 8, 2026 at 7:32 AM
Two new papers 📖 from our lab 1️⃣ What is epigenetics (and why X inactivation is?) rdcu.be/fhzSz and 2️⃣ A Baculovirus-based tool to express any gene of interest with high efficiency in Anopheles cells rdcu.be/fhzTy
Reposted by Guifeng Wei
Very importantly, one of the co-organisers @xrnchew.bsky.social is cycling to the meeting to raise awareness and funds for 'All things small and green' . Please join him or sponsor him!
gofund.me/f4068dc3c
gofund.me/f4068dc3c
Donate to Cycling from Exeter to the 3' end (in Oxford)!, organized by Steven West
I'll be cycling from Exeter to Oxford (about 160 miles/260 km) - hopefully … Steven West needs your support for Cycling from Exeter to the 3' end (in Oxford)!
gofund.me
April 27, 2026 at 8:49 PM
Very importantly, one of the co-organisers @xrnchew.bsky.social is cycling to the meeting to raise awareness and funds for 'All things small and green' . Please join him or sponsor him!
gofund.me/f4068dc3c
gofund.me/f4068dc3c
Stunning work — acquisition of IDRs in SON appears to have driven the evolution of nuclear speckles, enabling selective splicing of GC-rich intron–exon units.
Our most recent work on the “function and evolution” of #nuclear-speckles is now online at Cell @cp-cell.bsky.social
doi.org/10.1016/j.ce...
Read the thread👇 for the highlights of our findings.
doi.org/10.1016/j.ce...
Read the thread👇 for the highlights of our findings.
Redirecting
doi.org
March 1, 2026 at 10:12 PM
Stunning work — acquisition of IDRs in SON appears to have driven the evolution of nuclear speckles, enabling selective splicing of GC-rich intron–exon units.
super cool
New paper from my group in @science.org : "A small polymerase ribozyme that can synthesise itself and its complementary strand" www.science.org/doi/10.1126/...
Outstanding work by @edogia.bsky.social
Outstanding work by @edogia.bsky.social
February 14, 2026 at 11:44 AM
super cool
Reposted by Guifeng Wei
Yesterday we came together to honour Professor Raymond Dwek through the naming of the Raymond Dwek Seminar Room. Raymond's family (pictured) joined the celebration and Raymond was visibly touched, saying: "Thank you for this amazing gesture. I'm humbled by it, but I will treasure it."
February 6, 2026 at 2:29 PM
Yesterday we came together to honour Professor Raymond Dwek through the naming of the Raymond Dwek Seminar Room. Raymond's family (pictured) joined the celebration and Raymond was visibly touched, saying: "Thank you for this amazing gesture. I'm humbled by it, but I will treasure it."
Reposted by Guifeng Wei
Just out @natrevimmunol.nature.com
RNA-binding proteins and ribonucleoproteins as determinants of immunity
www.nature.com/articles/s41...
gives our perspective on this growing area of research. Special thanks to the editor Alexandra, for streamlining the manuscript.
RNA-binding proteins and ribonucleoproteins as determinants of immunity
www.nature.com/articles/s41...
gives our perspective on this growing area of research. Special thanks to the editor Alexandra, for streamlining the manuscript.
RNA-binding proteins and ribonucleoproteins as determinants of immunity - Nature Reviews Immunology
RNA-binding proteins (RBPs) considerably expand the information content of the genome and can determine the lifespan, localization and function of RNA, thereby controlling when, where and how much pro...
www.nature.com
January 27, 2026 at 7:32 AM
Just out @natrevimmunol.nature.com
RNA-binding proteins and ribonucleoproteins as determinants of immunity
www.nature.com/articles/s41...
gives our perspective on this growing area of research. Special thanks to the editor Alexandra, for streamlining the manuscript.
RNA-binding proteins and ribonucleoproteins as determinants of immunity
www.nature.com/articles/s41...
gives our perspective on this growing area of research. Special thanks to the editor Alexandra, for streamlining the manuscript.
an interesting work on how RNA regulatory elements control immune systems.
Researchers at the Crick, @cam.ac.uk and Sanquin Research Amsterdam have made a key discovery about how the immune system can switch from ‘on’ to ‘off’ so quickly.
The findings could offer potential new avenues for treating cancer or autoimmune conditions.
www.crick.ac.uk/news/2026-01...
The findings could offer potential new avenues for treating cancer or autoimmune conditions.
www.crick.ac.uk/news/2026-01...
A balancing act: preventing an overactive immune system
How do immune cells strike a balance, unleashing rapid attacks against pathogens or cancer, while avoiding damage to healthy cells? Research into an immune kill switch holds potential for controlling ...
www.crick.ac.uk
January 24, 2026 at 11:17 AM
an interesting work on how RNA regulatory elements control immune systems.