RNA modifications play an important role in biological processes. Mapping the diversity of RNA chemistry and studying the biological function of in...

In this Review, Dowdle and Lykke-Andersen discuss advances in our understanding of the machinery and mechanisms involved in the control of cytoplasmic mRNA decay and comment on implications for the de...

The snRNA Activating Protein Complex (SNAPc) mediates snRNA transcription by RNA Pol II and III. Here, the authors have used cryo-EM to resolve the structure of the SNAPc-bound RNA Pol III pre-initiat...

Published in Proceedings of the National Academy of Sciences of the United States of America

Abstract. Efficient purification of specific RNAs from lysates remains a major challenge. Existing methods, such as MS2 tagging or oligonucleotide hybridiz

SUMMARYRNA modifications in the tRNA, rRNA, and mRNA constitute a widespread layer of post-transcriptional gene regulation, commonly known as the epitranscriptome. In bacteria, tRNA and rRNA modificat...

Long interspersed element-1 (LINE-1) retrotransposons are the only active autonomous transposable elements in humans. They propagate by reverse transcribing their mRNA into new genomic locations by a ...

A new function of glycosylated RNAs has been discovered by Graziano et al. in which the glycosylation of RNA molecules shields acp³U RNA modification from triggering innate immune responses.

Abstract. LARP4 interacts with poly(A)-binding protein (PABP) to protect messenger RNAs (mRNAs) from deadenylation and decay, and recent data indicate it c

DNA double-strand break (DSB) induction leads to local transcriptional silencing at damage sites, raising the question: Why are RNA processing factors (RPFs), including splicing factors, rapidly recruited to these sites? Recent findings show that DSBs cluster in a chromatin compartment termed the ‘D compartment’, where DNA damage response (DDR) genes relocate and undergo transcriptional activation. Here, we propose two non-mutually exclusive models to elucidate the rationale behind the recruitment of RPFs to DSB sites. First, RPFs circulate through the D compartment to process transcripts of the relocated DDR genes. Second, the D compartment serves as a ‘post-translational modifications (PTMs) hub’, altering RPF activity and leading to the production of unique DNA damage-induced transcripts, which are essential for orchestrating the DDR.

Highly conserved mRNA 3′ UTRs act as co-translational chaperones for intrinsically disordered regions (IDRs), preventing inter-domain misfolding and enabling biogenesis of fully active proteins.

Li and Joshi et al. describe a molecular model by which RNA is stably presented on the surface of living cells. By forming complexes with RNA-binding proteins and heparan sulfate, cell-surface RNA can recruit immune receptors. Our findings lay the groundwork for investigating how cell-surface RNA contributes to immune modulation.

An ancient tRNA modification is used by mammalian cells to coordinate protein translation and adaptive signaling.

Variation in the composition of different ribosomes, termed ribosome heterogeneity, is a now well established phenomenon. However, the functional implications of this heterogeneity on the regulation of protein synthesis are only now beginning to be ...