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Glycans and glycoconjugates play a significant role in nearly all aspects of living organisms from birth through development to demise. For more than half

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ALT: Homer Simpson: Must Protect Sugar

PI: D. Wade Abbott (Agriculture and Agri-Food Canada) Keywords: TBD Rewilding of animals holds promise for restoring industrialized microbiomes back to their ancestral state, enhancing resilience and reducing the requirement for therapeutic interventions. Using a “two...

PI: Prof. Morten Andersen, A/Prof. Rebeca Kawahara (Macquarie University) Keywords: Sex-specific glycoproteome differences remain poorly understood despite emerging evidence pointing to protein glycosylation being a principal contributor to sex biology. Here, we present a comprehensive, tissue-resolved glycoproteome atlas of adult male and female C57BL/6N mice, integrating comparative transcriptomics, proteomics and glycoproteomics with sialic acid speciation and lectin microarray analyses across 19 mouse tissues. Quantitative profiling of >26,800 protein- and site-specific N-glycoforms covering 1,512 glycoproteins revealed that each tissue exhibits a distinct glycoproteome shaped by unique proteome and glycosylation traits. The tissue glycome diversity was governed by coordinated regulation of glyco-enzymes and protein expression patterns. Amongst many other glycophenotype-enzyme associations, multi-omics data integration indicated that tissue sialylation is governed by Cmas and Cmah suggesting that these are rate-limiting biosynthetic enzymes in the glycosylation machinery. Notably, we found that the salivary gland, liver, and kidney display pronounced sex-linked glycophenotypes driven by profound differences of fucosylation, sialylation and global protein levels in male and female mice. In contrast, the brain displayed extensive glycome diversity that was largely conserved between males and females, highlighting constraints within sex-specific glycosylation. An online database (https://igcore.cloud/GerOmics/atlas-viewer/) provides an interactive resource for exploring new molecular facets of sex-biased biology through the sex-linked glycoproteome variations that exist across mouse tissues. Sex-specific glycoproteome differences remain poorly understood despite emerging evidence pointing to protein glycosylation being a principal contributor to sex biology. Here, we present a comprehensi...

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