Juan P. Unfried
@jpunfried.bsky.social
RNA biologist. Postdoc in the Ulitsky Lab at the Weizmann Institute. I like historical fiction novels, photography, sports, wrapping presents, and plants.
Reposted by Juan P. Unfried
🆕 review with @jpunfried.bsky.social out in Nature SMB. Direct roles of lncRNAs in transcriptional activation. What do we understand about how lncRNAs lure Pol2 and set the stage for RNA production, and what do we still miss? www.nature.com/articles/s41...
Direct roles of long non-coding RNAs in transcription activation - Nature Structural & Molecular Biology
This Review discusses how long noncoding RNAs (lncRNAs) control transcription activation by RNA polymerase II, including how they are produced at active enhancers or chromatin-domain boundaries and ac...
www.nature.com
May 1, 2026 at 12:46 PM
🆕 review with @jpunfried.bsky.social out in Nature SMB. Direct roles of lncRNAs in transcriptional activation. What do we understand about how lncRNAs lure Pol2 and set the stage for RNA production, and what do we still miss? www.nature.com/articles/s41...
Any help will be much appreciated 🙏🏼
Q for #chromatin BSky - We're doing Cut&Run on cells where we want to halt transcription at various short time points after induction, with a cold PBS wash. This cold shock seems to hinder Ab uptake during, so our libraries look crappy. Any tips? Alt. methods to stop transcription?
January 2, 2025 at 6:38 PM
Any help will be much appreciated 🙏🏼
Reposted by Juan P. Unfried
sunburn is from ribosomes stalled by UV damage to RNA? super cool www.sciencedirect.com/science/arti...
The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo
Solar UVB light causes damage to the outermost layer of skin. This insult induces rapid local responses, such as dermal inflammation, keratinocyte cel…
www.sciencedirect.com
November 27, 2024 at 1:22 AM
sunburn is from ribosomes stalled by UV damage to RNA? super cool www.sciencedirect.com/science/arti...
🤩
TRISCO
A solid step closer to 3D #SpatialTranscriptomics 😎
Optimized probe-based 3D RNA imaging in whole organs
isHCR
Tris 500 mM
4'C
Validated probe list in Table S1
Brain Heart Lung Kidney
@peruhlen.bsky.social @science.org 2024
www.science.org/doi/10.1126/...
A solid step closer to 3D #SpatialTranscriptomics 😎
Optimized probe-based 3D RNA imaging in whole organs
isHCR
Tris 500 mM
4'C
Validated probe list in Table S1
Brain Heart Lung Kidney
@peruhlen.bsky.social @science.org 2024
www.science.org/doi/10.1126/...
November 26, 2024 at 8:41 PM
🤩
Reposted by Juan P. Unfried
Reposted by Juan P. Unfried
That is very interesting!
I wonder if mRNA decay factors play a role in this process.
For instance, Xrn1 was identified early on in yeast to function in strand exchange & homologous recombination.
Pat1 interacts physically with topoisomerase 2 in yeast.
I wonder if mRNA decay factors play a role in this process.
For instance, Xrn1 was identified early on in yeast to function in strand exchange & homologous recombination.
Pat1 interacts physically with topoisomerase 2 in yeast.
November 23, 2024 at 1:56 PM
That is very interesting!
I wonder if mRNA decay factors play a role in this process.
For instance, Xrn1 was identified early on in yeast to function in strand exchange & homologous recombination.
Pat1 interacts physically with topoisomerase 2 in yeast.
I wonder if mRNA decay factors play a role in this process.
For instance, Xrn1 was identified early on in yeast to function in strand exchange & homologous recombination.
Pat1 interacts physically with topoisomerase 2 in yeast.
Reposted by Juan P. Unfried
I’m thrilled to share a collaborative story from @Mendell_lab and Jan Erzberger labs, led by postdoc extraordinaire Xiaoqiang Zhu. We showed that in addition to their canonical decoding function, tRNAs play a key role in regulating mRNA stability during translation!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes
The CCR4-NOT complex is a major regulator of eukaryotic messenger RNA (mRNA) stability. Slow decoding during translation promotes association of CCR4-NOT with ribosomes, accelerating mRNA degradation....
www.science.org
November 22, 2024 at 6:09 PM
I’m thrilled to share a collaborative story from @Mendell_lab and Jan Erzberger labs, led by postdoc extraordinaire Xiaoqiang Zhu. We showed that in addition to their canonical decoding function, tRNAs play a key role in regulating mRNA stability during translation!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Juan P. Unfried
In this review @gracebower.bsky.social & I discuss recent evidence showing that, unlike canonical enhancers, long-range enhancers located hundreds of kb from their targets rely on additional mechanisms to ensure robust gene activation during mammal development. www.sciencedirect.com/science/arti...
November 22, 2024 at 8:20 PM
In this review @gracebower.bsky.social & I discuss recent evidence showing that, unlike canonical enhancers, long-range enhancers located hundreds of kb from their targets rely on additional mechanisms to ensure robust gene activation during mammal development. www.sciencedirect.com/science/arti...
Reposted by Juan P. Unfried
New on BioRxiv: We report a new, endogenous molecular clock in unmodified human cells and tissues. It is ticking away right now in almost every cell in your body.
Paper here: www.biorxiv.org/content/10.1...
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Paper here: www.biorxiv.org/content/10.1...
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November 20, 2024 at 2:55 PM
New on BioRxiv: We report a new, endogenous molecular clock in unmodified human cells and tissues. It is ticking away right now in almost every cell in your body.
Paper here: www.biorxiv.org/content/10.1...
1/
Paper here: www.biorxiv.org/content/10.1...
1/
Reposted by Juan P. Unfried
RNA Polymerase II subunits overexpressions induce genome instability and deregulate transcription https://www.biorxiv.org/content/10.1101/2024.11.20.622938v1
RNA Polymerase II subunits overexpressions induce genome instability and deregulate transcription https://www.biorxiv.org/content/10.1101/2024.11.20.622938v1
Independently of the pathways or circuits deregulated in cancer cells, these present altered transcr
www.biorxiv.org
November 21, 2024 at 5:17 AM
RNA Polymerase II subunits overexpressions induce genome instability and deregulate transcription https://www.biorxiv.org/content/10.1101/2024.11.20.622938v1
To read!
REX - a mammalian "range extender" element that can turn short-distance enhancers into long-distance enhancers.
New preprint from a collaboration led by Grace Bower and Evgeny Kvon.
doi.org/10.1101/2024...
New preprint from a collaboration led by Grace Bower and Evgeny Kvon.
doi.org/10.1101/2024...
November 21, 2024 at 3:30 PM
To read!