Ravi Teja Ravi
@ravi5693.bsky.social
Postdoctoral Research Associate @ The Rosalind Franklin Institute
Reposted by Ravi Teja Ravi
Tiny bacteria, fascinating biology 🦠 Great work by Marcel & Fabienne from Thomas Rudel’s lab @uni-wuerzburg.de using ExM to explore Chlamydia infection. @tillepprecht.ch from our group added cryo-ET insights—the tiniest bacteria we’ve ever milled!🔍 Congrats to the team
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Sphingolipids associate with the chlamydial nucleoid and mark developmental transitions in Chlamydia trachomatis - Nature Communications
During the developmental cycle of the pathogenic bacterium Chlamydia trachomatis, non-replicative bacterial cells (elementary bodies, EBs) alternate with replicative forms within membrane-bound inclus...
www.nature.com
October 2, 2026 at 1:24 PM
Tiny bacteria, fascinating biology 🦠 Great work by Marcel & Fabienne from Thomas Rudel’s lab @uni-wuerzburg.de using ExM to explore Chlamydia infection. @tillepprecht.ch from our group added cryo-ET insights—the tiniest bacteria we’ve ever milled!🔍 Congrats to the team
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Ravi Teja Ravi
Stunning cryo-ET analysis from the Harrison Lab capturing every stage of how non-membrane viruses infect animal cells
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
September 10, 2026 at 6:55 PM
Stunning cryo-ET analysis from the Harrison Lab capturing every stage of how non-membrane viruses infect animal cells
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Ravi Teja Ravi
We developed Adaptive Polishing for the consistent preparation of ~150 nm thin FIB-lamellae. This was done by using imaging feedback to adjust parameters for each lamella, or Adaptive Milling, rather than applying a fixed recipe.
Preprint: doi.org/10.64898/202...
Preprint: doi.org/10.64898/202...
September 23, 2026 at 12:47 PM
We developed Adaptive Polishing for the consistent preparation of ~150 nm thin FIB-lamellae. This was done by using imaging feedback to adjust parameters for each lamella, or Adaptive Milling, rather than applying a fixed recipe.
Preprint: doi.org/10.64898/202...
Preprint: doi.org/10.64898/202...
Reposted by Ravi Teja Ravi
Releasing TOMATO, the TOMogram Annotation TOol!
A lightweight Python GUI for going through hundreds of cryo-ET tomograms. Flag what's in each one, grade its quality, and end up with a table you can filter and act on.
github.com/alkemasimon/...
A lightweight Python GUI for going through hundreds of cryo-ET tomograms. Flag what's in each one, grade its quality, and end up with a table you can filter and act on.
github.com/alkemasimon/...
September 17, 2026 at 10:40 AM
Releasing TOMATO, the TOMogram Annotation TOol!
A lightweight Python GUI for going through hundreds of cryo-ET tomograms. Flag what's in each one, grade its quality, and end up with a table you can filter and act on.
github.com/alkemasimon/...
A lightweight Python GUI for going through hundreds of cryo-ET tomograms. Flag what's in each one, grade its quality, and end up with a table you can filter and act on.
github.com/alkemasimon/...
Reposted by Ravi Teja Ravi
Excited to share our new preprint on in situ chromatin structure of the inactive X chromosome in differentiated female mouse embryonic stem cells.
www.biorxiv.org/content/10.6...
🧵1/8
www.biorxiv.org/content/10.6...
🧵1/8
September 14, 2026 at 8:35 AM
Excited to share our new preprint on in situ chromatin structure of the inactive X chromosome in differentiated female mouse embryonic stem cells.
www.biorxiv.org/content/10.6...
🧵1/8
www.biorxiv.org/content/10.6...
🧵1/8
Reposted by Ravi Teja Ravi
Millions of beating cilia keep airways clean. At the base of each is the transition zone (TZ), a gate that controls which proteins enter and leave. In our paper out today in Science, we used cryo-FIB-ET to image the TZ inside human airway cells www.science.org/doi/10.1126/...
In situ structure of the human ciliary transition zone links linker defects to primary ciliary dyskinesia
The ciliary transition zone (TZ) regulates ciliary proteome composition, yet its molecular architecture, protein content, and contribution to motile ciliopathies remain poorly defined. We applied in situ cryo-electron tomography and subtomogram averaging ...
www.science.org
September 10, 2026 at 7:27 PM
Millions of beating cilia keep airways clean. At the base of each is the transition zone (TZ), a gate that controls which proteins enter and leave. In our paper out today in Science, we used cryo-FIB-ET to image the TZ inside human airway cells www.science.org/doi/10.1126/...
Reposted by Ravi Teja Ravi
Using advanced microscopy @piotrkolata.bsky.social @a-dsantos.bsky.social @tomdendooven.bsky.social @matteoall.bsky.social have identified the molecular anatomy & organisation of proteasomes in the sperm nucleus.
Read more about the study here: mrclmb.ac.uk/news-events/...
#LMBResearch 🧪
Read more about the study here: mrclmb.ac.uk/news-events/...
#LMBResearch 🧪
September 1, 2026 at 9:28 AM
Using advanced microscopy @piotrkolata.bsky.social @a-dsantos.bsky.social @tomdendooven.bsky.social @matteoall.bsky.social have identified the molecular anatomy & organisation of proteasomes in the sperm nucleus.
Read more about the study here: mrclmb.ac.uk/news-events/...
#LMBResearch 🧪
Read more about the study here: mrclmb.ac.uk/news-events/...
#LMBResearch 🧪
Reposted by Ravi Teja Ravi
One cell, one tomogram!
Our montage tomography workflow, MOSAIC, is out in Structure, and MontageMaker is on GitHub!
MOSAIC:
www.cell.com/structure/fu...
MontageMaker:
github.com/MPI-Dortmund...
Big thanks to @maikaboiero.bsky.social, Adriana Prajica, Gavin Rice, and @raunser-lab.bsky.social!
Our montage tomography workflow, MOSAIC, is out in Structure, and MontageMaker is on GitHub!
MOSAIC:
www.cell.com/structure/fu...
MontageMaker:
github.com/MPI-Dortmund...
Big thanks to @maikaboiero.bsky.social, Adriana Prajica, Gavin Rice, and @raunser-lab.bsky.social!
July 27, 2026 at 4:43 PM
One cell, one tomogram!
Our montage tomography workflow, MOSAIC, is out in Structure, and MontageMaker is on GitHub!
MOSAIC:
www.cell.com/structure/fu...
MontageMaker:
github.com/MPI-Dortmund...
Big thanks to @maikaboiero.bsky.social, Adriana Prajica, Gavin Rice, and @raunser-lab.bsky.social!
Our montage tomography workflow, MOSAIC, is out in Structure, and MontageMaker is on GitHub!
MOSAIC:
www.cell.com/structure/fu...
MontageMaker:
github.com/MPI-Dortmund...
Big thanks to @maikaboiero.bsky.social, Adriana Prajica, Gavin Rice, and @raunser-lab.bsky.social!
Reposted by Ravi Teja Ravi
With Cryo-ET/EM, the macromolecules we study (and biological questions we ask) are SNR-limited. Here, we share Nilas, a low-voltage milling method named after thin sea ice. Nilas produces thin (sub 50 nm in places!), minimally damaged lamellae. biorxiv.org/content/10.64898/2026.07.21.739890v1
Optimized cryo-FIB milling strategy to generate thin, minimally damaged biological lamellae
Focused ion beam (FIB)-milling has been adapted to thin frozen cells for visualization of macromolecular structures in situ with cryogenic electron microscopy. However, only a few large and abundant c...
biorxiv.org
July 25, 2026 at 3:08 PM
With Cryo-ET/EM, the macromolecules we study (and biological questions we ask) are SNR-limited. Here, we share Nilas, a low-voltage milling method named after thin sea ice. Nilas produces thin (sub 50 nm in places!), minimally damaged lamellae. biorxiv.org/content/10.64898/2026.07.21.739890v1
Reposted by Ravi Teja Ravi
How do 2 meters of DNA fit inside a nucleus you can barely see? 🧬 Our new study mapped individual nucleosomes inside human cells.
July 13, 2026 at 12:04 PM
How do 2 meters of DNA fit inside a nucleus you can barely see? 🧬 Our new study mapped individual nucleosomes inside human cells.
Reposted by Ravi Teja Ravi
We've made a cryoEM conference map + calendar + list (academic & industry) for the community. Let us know if we missed any! Have fun=)
cryoarcana.com/conferences
cryoarcana.com/conferences
July 1, 2026 at 8:43 PM
We've made a cryoEM conference map + calendar + list (academic & industry) for the community. Let us know if we missed any! Have fun=)
cryoarcana.com/conferences
cryoarcana.com/conferences
Reposted by Ravi Teja Ravi
From v0.2.0 MissAlignment now has an inference mode where you can apply the trained models from one dataset to a new dataset to skip training time. I haven't tested it extensively, so double check if the results look decent 😉
The program calls changed slightly, see here: github.com/warpem/miss-...
The program calls changed slightly, see here: github.com/warpem/miss-...
github.com
June 29, 2026 at 11:02 AM
From v0.2.0 MissAlignment now has an inference mode where you can apply the trained models from one dataset to a new dataset to skip training time. I haven't tested it extensively, so double check if the results look decent 😉
The program calls changed slightly, see here: github.com/warpem/miss-...
The program calls changed slightly, see here: github.com/warpem/miss-...
Reposted by Ravi Teja Ravi
What is the best way to distribute the electron dose across your tilt series in cryo ET?
Thanks to Maarten Tuijtel, we finally know --> head over to eLife for the publication! doi.org/10.7554/eLif...
Thanks to Maarten Tuijtel, we finally know --> head over to eLife for the publication! doi.org/10.7554/eLif...
June 26, 2026 at 10:47 AM
What is the best way to distribute the electron dose across your tilt series in cryo ET?
Thanks to Maarten Tuijtel, we finally know --> head over to eLife for the publication! doi.org/10.7554/eLif...
Thanks to Maarten Tuijtel, we finally know --> head over to eLife for the publication! doi.org/10.7554/eLif...
Reposted by Ravi Teja Ravi
The #EMBLProteomics course wrapped up last week, and the time just flew by
We hope participants leave with valuable knowledge and hands-on skills in proteomics sample preparation and analysis 📊
Thank you to all trainers, speakers, organisers, and participants for making it such a success!
We hope participants leave with valuable knowledge and hands-on skills in proteomics sample preparation and analysis 📊
Thank you to all trainers, speakers, organisers, and participants for making it such a success!
June 22, 2026 at 1:08 PM
The #EMBLProteomics course wrapped up last week, and the time just flew by
We hope participants leave with valuable knowledge and hands-on skills in proteomics sample preparation and analysis 📊
Thank you to all trainers, speakers, organisers, and participants for making it such a success!
We hope participants leave with valuable knowledge and hands-on skills in proteomics sample preparation and analysis 📊
Thank you to all trainers, speakers, organisers, and participants for making it such a success!
Reposted by Ravi Teja Ravi
Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Welcome to TomoGuide
A step-by-step Cryo-ET guide
tomoguide.github.io
May 6, 2025 at 4:27 PM
Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Reposted by Ravi Teja Ravi
Group leader Miguel Leung has been awarded the Young Scientists Award 2026 from @heinekenprizes.bsky.social . With his group, he investigates the 3D structure of protein complexes, tiny machines at work inside each of our cells. Congratulations, Miguel! Read more: www.hubrecht.eu/heineken-you...
June 9, 2026 at 9:17 AM
Group leader Miguel Leung has been awarded the Young Scientists Award 2026 from @heinekenprizes.bsky.social . With his group, he investigates the 3D structure of protein complexes, tiny machines at work inside each of our cells. Congratulations, Miguel! Read more: www.hubrecht.eu/heineken-you...
Reposted by Ravi Teja Ravi
The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes:
🖌️ Dozens more images with duplications or painting
🖨️ Hundreds of blots that all share the same background (behold slideshow below)
🖌️ Dozens more images with duplications or painting
🖨️ Hundreds of blots that all share the same background (behold slideshow below)
June 5, 2026 at 4:02 PM
The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes:
🖌️ Dozens more images with duplications or painting
🖨️ Hundreds of blots that all share the same background (behold slideshow below)
🖌️ Dozens more images with duplications or painting
🖨️ Hundreds of blots that all share the same background (behold slideshow below)
Reposted by Ravi Teja Ravi
We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇
May 27, 2026 at 10:14 AM
We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇
Reposted by Ravi Teja Ravi
Sub-cellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging
online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs
doi.org/10.1038/s415...
online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs
doi.org/10.1038/s415...
May 25, 2026 at 12:20 PM
Sub-cellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging
online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs
doi.org/10.1038/s415...
online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs
doi.org/10.1038/s415...
Reposted by Ravi Teja Ravi
MissAlignment is out!!! Our new software that improves reference-free tilt series alignment by mimicking human intuition. Check out Dimitry's beautiful thread for a quick summary!
And the preprint for more details: www.biorxiv.org/content/10.6...
The program: github.com/warpem/miss-...
And the preprint for more details: www.biorxiv.org/content/10.6...
The program: github.com/warpem/miss-...
May 3, 2026 at 8:17 AM
MissAlignment is out!!! Our new software that improves reference-free tilt series alignment by mimicking human intuition. Check out Dimitry's beautiful thread for a quick summary!
And the preprint for more details: www.biorxiv.org/content/10.6...
The program: github.com/warpem/miss-...
And the preprint for more details: www.biorxiv.org/content/10.6...
The program: github.com/warpem/miss-...
Reposted by Ravi Teja Ravi
Preprint 🧵!
I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition!
BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a
www.biorxiv.org/content/10.6... (1/6)
I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition!
BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a
www.biorxiv.org/content/10.6... (1/6)
April 25, 2026 at 7:25 AM
Preprint 🧵!
I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition!
BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a
www.biorxiv.org/content/10.6... (1/6)
I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition!
BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a
www.biorxiv.org/content/10.6... (1/6)
Reposted by Ravi Teja Ravi
Oocytes store large amounts of proteins to support the earliest developmental stages. In @nature.com, the Leung group describes the structure of the cytoplasmic lattice in unprecedented detail. At least 13 different proteins assemble into a stable storage complex. hubrecht.eu/oocyte-storage-complex/
April 22, 2026 at 7:20 AM
Oocytes store large amounts of proteins to support the earliest developmental stages. In @nature.com, the Leung group describes the structure of the cytoplasmic lattice in unprecedented detail. At least 13 different proteins assemble into a stable storage complex. hubrecht.eu/oocyte-storage-complex/