Assaying lung samples using spectral flow cytometry? Check out AutoSpectral:
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
October 28, 2025 at 8:25 AM
Assaying lung samples using spectral flow cytometry? Check out AutoSpectral:
A one minute primer on how #AutoSpectral improves spectral flow cytometry unmixing by using per-cell calculation of autofluroscence spectra.
Use our R package and get higher quality spectral data, easy as that!
www.biorxiv.org/content/10.1...
Use our R package and get higher quality spectral data, easy as that!
www.biorxiv.org/content/10.1...
March 31, 2026 at 12:04 PM
A one minute primer on how #AutoSpectral improves spectral flow cytometry unmixing by using per-cell calculation of autofluroscence spectra.
Use our R package and get higher quality spectral data, easy as that!
www.biorxiv.org/content/10.1...
Use our R package and get higher quality spectral data, easy as that!
www.biorxiv.org/content/10.1...
A small 🧵 encouraging people to publish protocols.
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level
The advent of spectral flow cytometry has seen a rapid rise in the complexity of flow cytometry experiments, allowing the construction of assays with at least 50 fluorescent parameters. To correctly d...
www.biorxiv.org
October 31, 2025 at 9:09 AM
A small 🧵 encouraging people to publish protocols.
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
The whole pipeline is available right now on GitHub. It includes comprehensive notes on every step from installation to utilisation.
github.com/DrCytometer/...
Hopefully soon (like with AutoSpill) it becomes standard on commercial platforms too. But don't wait - fix your data issues today!
github.com/DrCytometer/...
Hopefully soon (like with AutoSpill) it becomes standard on commercial platforms too. But don't wait - fix your data issues today!
GitHub - DrCytometer/AutoSpectral: Tools for Unmixing Spectral Flow Cytometry Data
Tools for Unmixing Spectral Flow Cytometry Data. Contribute to DrCytometer/AutoSpectral development by creating an account on GitHub.
github.com
October 28, 2025 at 8:06 AM
The whole pipeline is available right now on GitHub. It includes comprehensive notes on every step from installation to utilisation.
github.com/DrCytometer/...
Hopefully soon (like with AutoSpill) it becomes standard on commercial platforms too. But don't wait - fix your data issues today!
github.com/DrCytometer/...
Hopefully soon (like with AutoSpill) it becomes standard on commercial platforms too. But don't wait - fix your data issues today!
If you are interested in #AutoSpectral, described in the talk, the pre-print is here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
January 26, 2026 at 2:05 PM
If you are interested in #AutoSpectral, described in the talk, the pre-print is here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level https://www.biorxiv.org/content/10.1101/2025.10.27.684855v1
October 28, 2025 at 2:16 AM
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level https://www.biorxiv.org/content/10.1101/2025.10.27.684855v1
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level https://www.biorxiv.org/content/10.1101/2025.10.27.684855v1
October 28, 2025 at 2:16 AM
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level https://www.biorxiv.org/content/10.1101/2025.10.27.684855v1
'Here we developed AutoSpectral, a statistical approach to automate the production of minimal-residual error unmixing matrixes and pair multiple distinct multifluorescent spectra to individual cells within a mixed sample, via an R-based software tool.'
#Preprint #FlowCytometry
#Preprint #FlowCytometry
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level
The advent of spectral flow cytometry has seen a rapid rise in the complexity of flow cytometry experiments, allowing the construction of assays with at least 50 fluorescent parameters. To correctly d...
www.biorxiv.org
October 30, 2025 at 10:15 PM
'Here we developed AutoSpectral, a statistical approach to automate the production of minimal-residual error unmixing matrixes and pair multiple distinct multifluorescent spectra to individual cells within a mixed sample, via an R-based software tool.'
#Preprint #FlowCytometry
#Preprint #FlowCytometry
Three addition problems:
1)This unmixing solution still requires ideal positive-negative matching to find the right linear regression.
2) Cells have variation in background fluorescence
3) Fluorophores actually stuck on cells have variation in emissions
#AutoSpectral solves these!
1)This unmixing solution still requires ideal positive-negative matching to find the right linear regression.
2) Cells have variation in background fluorescence
3) Fluorophores actually stuck on cells have variation in emissions
#AutoSpectral solves these!
October 28, 2025 at 8:06 AM
Three addition problems:
1)This unmixing solution still requires ideal positive-negative matching to find the right linear regression.
2) Cells have variation in background fluorescence
3) Fluorophores actually stuck on cells have variation in emissions
#AutoSpectral solves these!
1)This unmixing solution still requires ideal positive-negative matching to find the right linear regression.
2) Cells have variation in background fluorescence
3) Fluorophores actually stuck on cells have variation in emissions
#AutoSpectral solves these!
First, pos-neg gating. Ideal controls are your actual antibodies on the actual cells. However different antibodies bind different cells (see: CD4 vs XCR1 below), meaning they have different backgrounds to control. AutoSpectral matches the FSC-SSC of each pos to a suitable neg: with results!
October 28, 2025 at 8:06 AM
First, pos-neg gating. Ideal controls are your actual antibodies on the actual cells. However different antibodies bind different cells (see: CD4 vs XCR1 below), meaning they have different backgrounds to control. AutoSpectral matches the FSC-SSC of each pos to a suitable neg: with results!
Thanks a lot for the informative thread! Looks very exciting
Are single stained cellular controls necessary or only better ? We are using rare human samples so it's difficult to cells for single color controls, can we still hope to see improvements with AutoSpectral unmixing ?
Are single stained cellular controls necessary or only better ? We are using rare human samples so it's difficult to cells for single color controls, can we still hope to see improvements with AutoSpectral unmixing ?
October 30, 2025 at 11:15 AM
Thanks a lot for the informative thread! Looks very exciting
Are single stained cellular controls necessary or only better ? We are using rare human samples so it's difficult to cells for single color controls, can we still hope to see improvements with AutoSpectral unmixing ?
Are single stained cellular controls necessary or only better ? We are using rare human samples so it's difficult to cells for single color controls, can we still hope to see improvements with AutoSpectral unmixing ?
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level www.biorxiv.org/content/10....
October 30, 2025 at 2:15 PM
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level www.biorxiv.org/content/10....