Biomanufacturing advances sustainable production and circular economy, while wastes-derived one-carbon (C1) compounds (e.g., CO2, methanol, formate and methane) expand substrate diversity, accelera...

The efficient valorization of gaseous C1 feedstocks, such as CO2, into liquid fuels presents a significant process engineering challenge. Here, we des…

We developed AneVO, a low-cost benchtop system for growing oxygen-sensitive bacteria, and used it to discover that combining methanol and carbon monoxide as feedstocks synergistically improves microbial growth and chemical production. This advance could enable more sustainable, efficient biomanufacturing of fuels and chemicals from renewable carbon sources.

Advances in carbon capture and gas fermentation technologies enable the sustainable production of one-carbon (C1) and C2 organic molecules, such as formate and acetate, by recycling CO2 and organic wastes. Refining C1 and C2 feedstocks into food and other organic ingredients is essential for establishing a carbon-neutral economy. However, current C1/C2-utilizing microbial refinery platforms have yet to achieve ideal carbon efficiency at industrial scales. We critically review progress in innovating metabolic systems across heterotrophic, photosynthetic, and gas fermentation microbial platforms including CO2-fixing and C1-assimilating pathways. Innovative mixotrophic metabolisms, such as photo-mixotrophic and litho-mixotrophic platforms, as well as autotrophic metabolism in heterotrophic platforms, can revolutionize global carbon cycles and enable a sustainable bioeconomy by recycling carbon waste into bioingredients with unprecedented efficiency.

The conversion of C1 molecules (single-carbon species) into Cn products (carbon chains) is a key challenge for developing sustainable chemical feedstocks to replace fossil resources. Carbohydrates, a ...

The conversion of C1 molecules (single-carbon species) into Cn products (carbon chains) is a key challenge for developing sustainable chemical feedstocks to replace fossil resources. Carbohydrates, a ...

The conversion of C1 molecules (single-carbon species) into Cn products (carbon chains) is a key challenge for developing sustainable chemical feedstocks to replace fossil resources. Carbohydrates, a ...

The conversion of C1 molecules (single-carbon species) into Cn products (carbon chains) is a key challenge for developing sustainable chemical feedstocks to replace fossil resources. Carbohydrates, a ...

The conversion of C1 molecules (single-carbon species) into Cn products (carbon chains) is a key challenge for developing sustainable chemical feedstocks to replace fossil resources. Carbohydrates, a ...

The conversion of C1 molecules (single-carbon species) into Cn products (carbon chains) is a key challenge for developing sustainable chemical feedstocks to replace fossil resources. Carbohydrates, a ...

The conversion of C1 molecules (single-carbon species) into Cn products (carbon chains) is a key challenge for developing sustainable chemical feedstocks to replace fossil resources. Carbohydrates, a ...

Single carbon (C1) substrates are gaining importance as future feedstocks for the production of bio-based chemicals. Carbon dioxide, a major greenhouse gas, offers a promising alternative to the tradi...

In this review, we summarize the challenges in establishing synthetic pathways for assimilation of C1 feedstocks in microbes and how to solve these issues

Soluble C1 feedstocks, such as formate and methanol, have gained attention as sustainable substrates for biotechnology, with the potential to reduce greenhouse gas emissions and reliance on sugar-based resources. Despite their promise, the metabolic ...

Soluble C1 feedstocks, such as formate and methanol, have gained attention as sustainable substrates for biotechnology, with the potential to reduce greenhouse gas emissions and reliance on sugar-based resources. Despite their promise, the metabolic ...

The utilization of C1 feedstocks as carbon substrates for chemical production holds significant potential for improving sustainability. However, current investment priorities continue to favor conventional petroleum refineries, which benefit from superior cost competitiveness, reliable material supply chains, and lower financial...

One carbon compounds such as CO2, methanol and formate are cost-effective and environmentally friendly microbial feedstocks for biomanufacturing. Here, the authors report the oxygen tolerant reductive...

One-carbon (C1) substrates are attractive feedstocks for biological upgrading as part of a circular, carbon-negative bioeconomy. Nature has evolved a …

One-carbon (C1) substrates are attractive feedstocks for biological upgrading as part of a circular, carbon-negative bioeconomy. Nature has evolved a …