Myocilin marks the glia limitans superficialis Astrocyte

Re-analysis of brain-wide #SpatialTranscriptomics & sc-/sn-RNAseq data➡️
Myoc+ 2% of total astrocytes covering brain/spinal cord surface

@philiphasel.bsky.social
@liddelowsa.bsky.social
#CellRep 2025
www.cell.com/cell-reports...

Amazing #2PM real-time morpho-dynamic responses of microglia to laser-induced parenchymal/vascular injury in🐭🧠

Age-dependent reaction speed
👉Parenchymal injury
Adult > Aged/Neonatal
👉Vascular injury
Adult/Aged > Neonatal

@vcoelhosantoslab.bsky.social #CellRep 2025
www.cell.com/cell-reports...

Adipocyte-to-Fibroblast transition in Skin wound healing 👹

Adipoq-CreER;Pdgfra/b-Flp0;R26-Tomato or AdipoChaser🐭

👉Contributing to 1~4% wound cells
👉Up to 80% PDGFRa+ adipocyte-derived fibroblast-like cells are perilipin-negative
👉⏫Skin Wound healing

#CellRep 2026
www.cell.com/cell-reports...

#CADASIL #CerebralSmallVesselDisease

Great comparative phenomics &🧠vessel multiomics
1⃣scRNAseq 2⃣proteomics 3⃣phosphoproteomics

Pericyte vs Smooth Muscle Cell Notch3 R170C🐭

Surprisingly, Notch3 signaling per se was unaltered in either🐭

@rrust.bsky.social #CellRep 2026
www.cell.com/cell-reports...

SETD2 H3K36 methyltransferase
Pancreas development
e11.5/13.5/15.5

PDX+ pancreatic progenitor Setd2 KO🐭
👉⏫Exocrine cell proliferation + perturbed tip-to-acinar trajectory
👉⏬α/β Islets ⏫blood glucose

NKX2.2➡️endocrine specification

#CellRep 2024
www.sciencedirect.com/science/arti...

High-confidence glycosomal membrane protein inventory unveils trypanosomal peroxin PEX15 #CellRep #MassSpec www.cell.com/cell-reports...

RE-read this important paper on small GTPases (Rac/Rho) in Smooth Muscle Cell responses to Matrix Stiffness

Distinct Roles of YAP & TAZ in Mechanotransduction (to SMC priliferation)😎

PAGE gel matrix 0.1-50 kPa

Richard Assoian lab #CellRep 2021
www.cell.com/cell-reports...

SIRT6 physically binds to PPARγ & suppress its transcriptional activity on Fatty acid metabolic genes (CD36, caveolin-1, FATP1, FABP3, VLDLR)

⬇️PPARγ occupancy at target gene promoters, independent of H3K9 and H3K56 acetylation

#CellRep 2021
www.sciencedirect.com/science/arti...

#BloodBrainBarrier

Induced #EndothelialCell Claudin 5 KO (but not Cldn5 -/+) in adult🐭➡️ #BBB leakage (1~10 kDa, but not 70 kD)

Cldn5-null ECs (15% of total ECs) have minimal vs Cldn5-intact ECs➡️non-cell-autonomous impact secondary to BBB leak

#CellRep 2024
www.sciencedirect.com/science/arti...

An integrated proteomics approach identifies phosphorylation sites on viral and host proteins that regulate West Nile virus infection #CellRep #MassSpec www.cell.com/cell-reports...

#YAP #Mechanoptosis #RigiditySensing

pTyr357 YAP, phospho by c-Abl/Src in #FocalAdhesion then translocated to Nuclei, ➡️Apoptosis of MDA-Tpm2.1; MEF on soft matrix 0.25kPa

Cellular sensing of #MatrixStiffness dictates proliferation/apoptosis switch of YAP

#CellRep 2024
www.cell.com/cell-reports...

#PoloLikeKinase1 overexpression🐭->
#ChromosomalInstability #Aneuploidy #Ployploidy #Senescence in #BreastCancer
⏫SASP (IL1RN/IL6/CXCL1)
⏫PD-L1
Reshaped immune niche:
⏫Treg⏫Dendritic cell⏫Arg1+TAM⏬NK

PLK1-hi👤breast tumors->⏫Immunopressive features

#CellRep 2023
www.sciencedirect.com/science/arti...