#Chromochloris
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biorxiv-sysbio.bsky.social
Investigating Overflow Metabolism in Heterotrophic Cultures of the Green Alga Chromochloris zofingiensis https://www.biorxiv.org/content/10.1101/2025.10.20.683564v1
October 22, 2025 at 12:04 AM
tkozawa.bsky.social
Great preprint by Upadhyaya et al. (2025) on using a #multiomics approach to study #PlantTOR signaling in the unicellular green alga #Chromochloris zofingiensis, particularly showing the upregulation of #AminoAcid biosynthesis pathways during TOR inhibition with AZD8055.
#Algae #Phosphoproteomics 🔗⬇️
Fig. 8. Summary of the mechanism of TOR inhibition-dependent accumulation of amino acids in Chromochloris zofingiensis. TOR inhibition by AZD8055 induces the expression of transcription factors G2- like, HSF, and bHLH, which bind to amino acid biosynthesis and nitrogen metabolism genes to potentially regulate their transcription. This process, which is independent of glucose-TOR signaling, leads to an accumulation of amino acids during TOR inhibition. Glucose addition via hexokinase1 (HXK1) also upregulates amino acid biosynthesis. Fig. 1. TOR inhibition prevents glucose-driven growth and suppresses photosynthesis in the green alga Chromochloris zofingiensis. (A) Representative C. zofingiensis (WT) cultures over the 96 h experiment with the following treatments: control, TOR inhibition by 1 µM AZD8055, 100 mM glucose addition, and 100mM glucose + 1 µM AZD treatment. (B) Cell density and (C) maximum PSII photosynthetic efficiency (Fv/Fm). For (B) and (C), datapoints represent biological replicates with lines representing means (n = 3). (D) PCA of WT 1 h and 24 h transcriptome, proteome, and metabolome with treatments: control, 1 µM AZD, 15 mM glucose, 15 mM glucose + 1 µM AZD, and 15 mM sorbitol as an osmotic control. For the transcriptome, normalized log2-transformed counts of 500 genes with highest variance were considered. For the proteome and the metabolome, the raw intensity values were log2-transformed, and proteins or metabolites with highest variance were used to calculate and plot the PCA. Modified Fig. 2. Phosphoproteomics of TOR-inhibited Chromochloris zofingiensis identifies novel TOR players. (B) The log2 fold changes of TOR-dependent phosphosites between TOR inhibition by 1 µM AZD, 15 mM glucose addition, 15 mM glucose + 1 µM AZD, and 15 mM sorbitol-treated C. zofingiensis (WT) cultures at 1 h and 24 h from cluster 4 were labelled according to their protein symbols and phosphorylated Ser/Thr residue on the left and by their ortholog name identified on the right. The phosphopeptides were labelled if they are known conserved TOR targets as “known targets” and if they are unknown as “novel targets”. RbcL, MAPK, PP2C, PP2C, and SnRK2 were identified as putative novel conserved TOR targets. White boxes in the heatmap indicate that the phosphopeptides were not detected. Data represent means of fold changes log2 intensities of phosphopeptides (n = 3-4 biological replicates).
October 2, 2025 at 4:27 PM
vinayshukla.bsky.social
Iron rescues glucose-mediated photosynthesis repression during lipid accumulation in the green alga Chromochloris zofingiensis
www.nature.com/articles/s41...
#plantscience
Iron rescues glucose-mediated photosynthesis repression during lipid accumulation in the green alga Chromochloris zofingiensis - Nature Communications
The cofactor iron is essential for cellular energy metabolism. Proteomics reveals how the interplay of iron limitation with glucose signaling underlies how a green alga turns off photosynthesis during...
www.nature.com
July 21, 2024 at 6:21 PM
biorxiv-microbiol.bsky.social
Impact of Cell Size, Light Wavelengths, and Intensities on Growth, Oxygen Production, and Consumption Rates of Chromochloris zofingiensis and Haematococcus lacustris https://www.biorxiv.org/content/10.1101/2025.11.18.689143v1
November 20, 2025 at 5:17 PM
biorxivpreprint.bsky.social
Impact of Cell Size, Light Wavelengths, and Intensities on Growth, Oxygen Production, and Consumption Rates of Chromochloris zofingiensis and Haematococcus lacustris https://www.biorxiv.org/content/10.1101/2025.11.18.689143v1
November 20, 2025 at 5:17 PM
biorxivpreprint.bsky.social
Investigating Overflow Metabolism in Heterotrophic Cultures of the Green Alga Chromochloris zofingiensis https://www.biorxiv.org/content/10.1101/2025.10.20.683564v1
October 22, 2025 at 12:04 AM
jcamthrash.bsky.social
Investigating Overflow Metabolism in Heterotrophic Cultures of the Green Alga Chromochloris zofingiensis www.biorxiv.org/content/10.1... #jcampubs
October 22, 2025 at 4:15 AM