This dataset compiles experimentally validated splice leader (SL) sequences from diverse eukaryotic taxa, providing a comparative reference for studies on trans-splicing and RNA processing. Version 7 represents a major update from v3 (January 2025), nearly doubling the number of entries from 112 to 210 sequences (+98 entries, +87%).Taxonomic coverage has been significantly expanded to now include 11 phyla: Euglenozoa, Nematoda, Platyhelminthes, Cnidaria, Rotifera, Chordata, Alveolata, and four newly added phyla — Arthropoda (Copepoda), Chaetognatha (Sagittoidea), Cryptista (Cryptomonada), and Ctenophora (Tentaculata/Nuda). The largest expansions were in Nematoda, Cnidaria, and Chaetognatha.The dataset schema was restructured to improve phylogenetic resolution, replacing the single "Family" field with a two-level "Phylum / Subgroup" classification. A formal SL sequence naming convention was introduced, with 170 of 210 entries carrying standardized identifiers. Each entry includes the SL sequence (5' to 3'), sequence length, primary literature reference (DOI and PMID), and contributor information.

To investigate the basis for kinesin-2 autoregulation, we generated AlphaFold2 and AlphaFold3 models for a range of kinesin-2 sequences from across eukaryotic supergroups51 (TSAR, Haptista, Cryptista, Archaeplastida, Amorphea and Excavates), including heteromeric motors (Homo sapiens Kif3A–Kif3B, C. reinhardtii FLA8–FLA10 and...

Belongs within: Eukaryota.Contains: Vendophyceae, Ischyrospongia, Haptista, Corbihelia, Sar, Cryptista, Rhodophyta, Glaucophyta, Chlorophyta, Streptophyta. The Diaphoretickes are a clade of eukaryotes supported by molecular phylogenies that might be described as comprising the major algal lineages and related taxa. Cavalier-Smith et al. (2015) defined an equivalent clade under the name Corticata as including all descendents of the… Continue reading Diaphoretickes

Goniomonads are heterotrophic representatives of Cryptista, sister lineage to the phototrophic Cryptists. Goniomonads are morphologically and genetically diverse, comprising nine genera described so f...

The 1270 surveyed species encompassed members of seven eukaryotic supergroups: Obazoa (n = 1067), Amoebozoa (n = 9), excavates (n = 21), Cryptista (n = 3), Archaeplastida (n = 82), Haptista (n = 1), and Stramenopiles, Alveolata, and Rhizaria (SAR)...