IE=Edge

Tick-borne encephalitis (TBE), and other (non-tick) lymphocytic meningitis diseases (LMD) are characterized by the presence of inflammatory processes differently located and with different intensity. Inflammatory processes in TBE and LMD may influence the activity of some catabolic enzymes including lysosomal exoglycosidases: N-acetyl-{beta}-D-hexosaminidase (HEX), its isoenzymes A (HEX A) and B (HEX B) as well as {beta}-galactosidase (GAL), -mannosidase (MAN), - fucosidase (FUC) and {beta}- glucuronidase (GLU). The aim of the study was to investigate the activity of lysosomal exoglycosidases in the serum and urine of patients with TBE and LMD. Material and methods: Examined group included 41 patients: a/ 25 patients with TBE, b/ 16 patients with LMD. Control group included 25 patients in which Borrelia burgdoferi and inflammatory state in central nervous system were excluded. Lysosomal exoglycosidases activity were assayed in the serum and urine by the colorimetric method, with the p-nitrophenyl derivative of appropriate sugar as substrate. Results: Concentration of activity (pKat/mL) of HEX, HEX A, HEX B and GLU increases significantly in serum of patients with TBE, and LMD as well as GAL, MAN and FUC serum activity in LMD patients in comparison to serum of patients from the control group (p

Tick-borne encephalitis (TBE), and other (non-tick) lymphocytic meningitis diseases (LMD) are characterized by the presence of inflammatory processes differently located and with different intensity. Inflammatory processes in TBE and LMD may influence the activity of some catabolic enzymes including lysosomal exoglycosidases: N-acetyl-{beta}-D-hexosaminidase (HEX), its isoenzymes A (HEX A) and B (HEX B) as well as {beta}-galactosidase (GAL), -mannosidase (MAN), - fucosidase (FUC) and {beta}- glucuronidase (GLU). The aim of the study was to investigate the activity of lysosomal exoglycosidases in the serum and urine of patients with TBE and LMD. Material and methods: Examined group included 41 patients: a/ 25 patients with TBE, b/ 16 patients with LMD. Control group included 25 patients in which Borrelia burgdoferi and inflammatory state in central nervous system were excluded. Lysosomal exoglycosidases activity were assayed in the serum and urine by the colorimetric method, with the p-nitrophenyl derivative of appropriate sugar as substrate. Results: Concentration of activity (pKat/mL) of HEX, HEX A, HEX B and GLU increases significantly in serum of patients with TBE, and LMD as well as GAL, MAN and FUC serum activity in LMD patients in comparison to serum of patients from the control group (p

Scientific Reports - A novel broad specificity fucosidase capable of core α1-6 fucose release from N-glycans labeled with urea-linked fluorescent dyes

Here we report evidence that new aminoquinoline N-glycan fluorescent labels interfere with the release of core α(1–6) fucose from N-glycans by bovine kidney α-l-fucosidase (BKF). BKF is a commonly emp...

We present the first detailed structure of a newly discovered fucosidase that has been shown to remove terminal fucose from fucoidan, uncovering unique features that set it apart from other known fucosidases and suggesting functional diversity among its homologs.

In a groundbreaking discovery that sheds new light on the replication mechanisms of prominent gastrointestinal pathogens, researchers have elucidated the critical role of fucosidase enzymes in the

Bacterial vaginosis (BV) is a polymicrobial infection of the female reproductive tract (FRT). BV is

Expression of the glycosidases Fuc23586, Fuc53726, AlfB13, AlfC13, AfcA8, and LnbB15 was carried out in E. coli BL21. Bacterial cultures carrying expression plasmids were grown overnight at 37 °C with agitation (250 rpm) in 5 mL of 2 × YT...

In a groundbreaking study published in BMC Cancer, researchers have illuminated the pivotal role of α-L-fucosidase isoenzymes, FUCA1 and FUCA2, as prognostic markers in gliomas, a group of aggressive brain tumors. Gliomas, particularly lethal in their most malignant form known as glioblastomas, have long posed a challenge for effective diagnosis and treatment. This comprehensive investigation melds vast genomic data with experimental validation, revealing a new dimension in understanding glioma biology and patient prognosis.