the HIV envelope protein, the V3-glycan epitope. Many prior attempts to target the V3-glycan epitope of Env have worked under the assumption that antibodies must interact with a specific sugar, the Asn332 glycan, to neutralize HIV. As a result, immunogens were designed to preserve this glycan.
May 14, 2026 at 4:06 PM
the HIV envelope protein, the V3-glycan epitope. Many prior attempts to target the V3-glycan epitope of Env have worked under the assumption that antibodies must interact with a specific sugar, the Asn332 glycan, to neutralize HIV. As a result, immunogens were designed to preserve this glycan.
By deep characterisation of 186 N-glycan standards, we @thencfg.bsky.social have made the most comprehensive targeted LC-MS glycomic assay to date.
Non-reduced native glycan analysis is key.
We hope this enables anyone with QqQ and interest in #glycotime to measure specific N-glycan structures.
Non-reduced native glycan analysis is key.
We hope this enables anyone with QqQ and interest in #glycotime to measure specific N-glycan structures.
June 10, 2025 at 1:11 PM
By deep characterisation of 186 N-glycan standards, we @thencfg.bsky.social have made the most comprehensive targeted LC-MS glycomic assay to date.
Non-reduced native glycan analysis is key.
We hope this enables anyone with QqQ and interest in #glycotime to measure specific N-glycan structures.
Non-reduced native glycan analysis is key.
We hope this enables anyone with QqQ and interest in #glycotime to measure specific N-glycan structures.
animal models showing that the Asn332 glycan is underrepresented in transmitted or founder viruses, and infection with Asn332-glycan-deficient viruses has been associated with the later development of Asn332-dependent bNAbs in humans. In addition, two human V3-glycan bNAbs, EPTC112 and 007, were
May 14, 2026 at 4:06 PM
animal models showing that the Asn332 glycan is underrepresented in transmitted or founder viruses, and infection with Asn332-glycan-deficient viruses has been associated with the later development of Asn332-dependent bNAbs in humans. In addition, two human V3-glycan bNAbs, EPTC112 and 007, were
infectious disease. An interesting fact that some may not know is that viruses, including SARS-CoV-2, use sugar molecules on their surfaces to hide from the immune system, much like a protective coating (sugar coatings aren't only for candies). Now, researchers have developed a UNIVERSAL coronavirus
March 27, 2025 at 4:03 PM
infectious disease. An interesting fact that some may not know is that viruses, including SARS-CoV-2, use sugar molecules on their surfaces to hide from the immune system, much like a protective coating (sugar coatings aren't only for candies). Now, researchers have developed a UNIVERSAL coronavirus
found to neutralize HIV without contacting the Asn332 glycan. This provided evidence that Asn332-glycan-independent bNAbs COULD develop in humans. To test their hypothesis, researchers designed WIN332 as an Asn332-glycan-deficient priming immunogen and evaluated it in nonhuman primates. Their data
May 14, 2026 at 4:08 PM
found to neutralize HIV without contacting the Asn332 glycan. This provided evidence that Asn332-glycan-independent bNAbs COULD develop in humans. To test their hypothesis, researchers designed WIN332 as an Asn332-glycan-deficient priming immunogen and evaluated it in nonhuman primates. Their data
Love the title and my goodness what a glycan tree 🍝
December 17, 2024 at 2:35 AM
Love the title and my goodness what a glycan tree 🍝
Analysis of these responses showed that WIN332 elicited antibodies closely resembling potent human V3-glycan bNAbs. The data also found two distinct classes of antibodies: type-I antibodies that depend on the Asn332 glycan, AND a new type-II class of antibodies that remain glycan independent.
May 14, 2026 at 4:09 PM
Analysis of these responses showed that WIN332 elicited antibodies closely resembling potent human V3-glycan bNAbs. The data also found two distinct classes of antibodies: type-I antibodies that depend on the Asn332 glycan, AND a new type-II class of antibodies that remain glycan independent.
details how researchers engineered a protein that removes the N332-glycan completely to produce their candidate WIN332, an unprecedented approach to current vaccine efforts that have focused on preserving N332-glycan. The development of WIN332 potentially addresses one of the big challenges in HIV
May 14, 2026 at 4:04 PM
details how researchers engineered a protein that removes the N332-glycan completely to produce their candidate WIN332, an unprecedented approach to current vaccine efforts that have focused on preserving N332-glycan. The development of WIN332 potentially addresses one of the big challenges in HIV
Researchers, however, deliberately removed the Asn332 glycan completely to create WIN332, thinking that early antibody precursors may not require it and that glycan dependence could develop later through somatic hypermutation. This line of thinking is supported by earlier studies in both humans and
May 14, 2026 at 4:06 PM
Researchers, however, deliberately removed the Asn332 glycan completely to create WIN332, thinking that early antibody precursors may not require it and that glycan dependence could develop later through somatic hypermutation. This line of thinking is supported by earlier studies in both humans and
Nice work by @cellsensing.bsky.social Glycan recognition by a plant damage-sensing immune receptor www.science.org/doi/10.1126/...
Glycan recognition by a plant damage-sensing immune receptor
Pathogens target and degrade the extracellular matrix surrounding plant cells. A central question is how cell wall-derived damage-associated molecular patterns (DAMPs) are recognized and integrated to...
www.science.org
September 11, 2026 at 7:00 AM
Nice work by @cellsensing.bsky.social Glycan recognition by a plant damage-sensing immune receptor www.science.org/doi/10.1126/...
Our manuscript describing @riley-research.bsky.social's GlyCounter informatics tool is now in press at Molecular and Cellular Proteomics. Congrats to first-author Katie Kothlow, her first first-author paper!
The manuscript is available here: www.mcponline.org/article/S153...
The manuscript is available here: www.mcponline.org/article/S153...
October 16, 2025 at 6:05 PM
Our manuscript describing @riley-research.bsky.social's GlyCounter informatics tool is now in press at Molecular and Cellular Proteomics. Congrats to first-author Katie Kothlow, her first first-author paper!
The manuscript is available here: www.mcponline.org/article/S153...
The manuscript is available here: www.mcponline.org/article/S153...
Our new paper in print describes a new approach: release and analysis of N-glycans in less than 24 hours. A perfect approach for those that are glycan-curious as it does not require changing any of your proteomics buffers! 1/5
#glycotime #teammassspec
pubs.acs.org/doi/10.1021/...
#glycotime #teammassspec
pubs.acs.org/doi/10.1021/...
Swift Universal Glycan Acquisition (SUGA) Enables Quantitative Glycan Profiling across Diverse Sample Types
The ability to rapidly analyze complex mixtures of glycans derived from glycoproteins is important, but techniques are often laborious and require multiple glycan derivatization steps. Here, we descri...
pubs.acs.org
February 21, 2025 at 6:55 AM
Our new paper in print describes a new approach: release and analysis of N-glycans in less than 24 hours. A perfect approach for those that are glycan-curious as it does not require changing any of your proteomics buffers! 1/5
#glycotime #teammassspec
pubs.acs.org/doi/10.1021/...
#glycotime #teammassspec
pubs.acs.org/doi/10.1021/...
HIV-1 antibody-mediated neutralization via a dual-glycan clamp
HIV-1 antibody-mediated neutralization via a dual-glycan clamp
Gorman et al. isolate VRC49, the second Fab-dimerized glycan-reactive, broadly neutralizing antibody recovered from an HIV-infected human since 2G12. Cryo-EM reveals a distinct dimerization mechanism through a CDRH3 disulfide bond forming a wedge that is clamped by nearby glycans, expanding our understanding of how antibodies target the HIV glycan shield.
dlvr.it
October 2, 2026 at 12:35 PM
HIV-1 antibody-mediated neutralization via a dual-glycan clamp
A new jump forward in cancer immunotherapy blocking glycan sugar molecules (antibodies to lectin, AbLec) that releases the immune system brakes against many types of tumors @carolynbertozzi.bskyverified.social @jcstark.bsky.social www.nature.com/articles/s41...
December 16, 2025 at 3:11 PM
A new jump forward in cancer immunotherapy blocking glycan sugar molecules (antibodies to lectin, AbLec) that releases the immune system brakes against many types of tumors @carolynbertozzi.bskyverified.social @jcstark.bsky.social www.nature.com/articles/s41...
glycan structures present on viruses, researchers are uncovering new pathways to enhance vaccine efficacy. Their low-sugar vaccine not only potentially presents a solution to tackling rapidly mutating viruses BUT also introduces a paradigm shift in vaccine design, where carbohydrate structures are
March 27, 2025 at 4:16 PM
glycan structures present on viruses, researchers are uncovering new pathways to enhance vaccine efficacy. Their low-sugar vaccine not only potentially presents a solution to tackling rapidly mutating viruses BUT also introduces a paradigm shift in vaccine design, where carbohydrate structures are
Review @annualreviews.bsky.social @gabyrabi.bsky.social
Glycan-Binding Proteins in Immunity
www.annualreviews.org/content/jour...
Glycan-Binding Proteins in Immunity
www.annualreviews.org/content/jour...
February 26, 2026 at 10:12 PM
Review @annualreviews.bsky.social @gabyrabi.bsky.social
Glycan-Binding Proteins in Immunity
www.annualreviews.org/content/jour...
Glycan-Binding Proteins in Immunity
www.annualreviews.org/content/jour...
to develop vaccines for the treatment of various cancers. They recently published two studies on glycan targets on cancer cells and enzymes linked to the synthesis of glycans on cancer cells in the Journal of the American Chemical Society. The research community eagerly anticipates the outcomes of
March 27, 2025 at 4:15 PM
to develop vaccines for the treatment of various cancers. They recently published two studies on glycan targets on cancer cells and enzymes linked to the synthesis of glycans on cancer cells in the Journal of the American Chemical Society. The research community eagerly anticipates the outcomes of
New cryoEM structure of zoonotic H5N1 from Peter Kwong's lab curiously shows the glycan at N169 occupying the HA receptor binding site of a neighboring promoter #glycotime 🧪
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
December 9, 2024 at 5:57 PM
New cryoEM structure of zoonotic H5N1 from Peter Kwong's lab curiously shows the glycan at N169 occupying the HA receptor binding site of a neighboring promoter #glycotime 🧪
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The study has been published in Nature Immunology. YES, it is PEER-REVIEWED.
• www.nature.com/articles/s41...
NOTE: THIS IS PRECLINICAL.
🧪🧵⬇️
• www.nature.com/articles/s41...
NOTE: THIS IS PRECLINICAL.
🧪🧵⬇️
May 14, 2026 at 4:02 PM
The study has been published in Nature Immunology. YES, it is PEER-REVIEWED.
• www.nature.com/articles/s41...
NOTE: THIS IS PRECLINICAL.
🧪🧵⬇️
• www.nature.com/articles/s41...
NOTE: THIS IS PRECLINICAL.
🧪🧵⬇️
A glycan that performs catalysis
Check out this Research Briefing in @natchem.nature.com
@mpici.bsky.social
www.nature.com/articles/s41...
Check out this Research Briefing in @natchem.nature.com
@mpici.bsky.social
www.nature.com/articles/s41...
A glycan that performs catalysis - Nature Chemistry
In nature, catalytic functions are typically restricted to proteins and ribozymes. Now, a synthetic glycan capable of catalysing a chemical reaction is demonstrated. The folded glycan scaffold positio...
www.nature.com
April 8, 2025 at 1:31 PM
A glycan that performs catalysis
Check out this Research Briefing in @natchem.nature.com
@mpici.bsky.social
www.nature.com/articles/s41...
Check out this Research Briefing in @natchem.nature.com
@mpici.bsky.social
www.nature.com/articles/s41...
Another piece of the microbe-mucin interactions puzzle 🧩:
Enzyme extracts of our favourite mucin glycan degraders cleave different bonds that occur in mucins. 🍬
Expression of glycoside hydrolases also differs per species. ✂️
To each their own! 😋
#glycotime #microsky #mucin
doi.org/10.1093/glyc...
Enzyme extracts of our favourite mucin glycan degraders cleave different bonds that occur in mucins. 🍬
Expression of glycoside hydrolases also differs per species. ✂️
To each their own! 😋
#glycotime #microsky #mucin
doi.org/10.1093/glyc...
Efficient mucin O-glycan degradation by specific mucin degrading intestinal bacteria: towards understanding enzyme-glycan interactions
Abstract. Intestinal mucin glycan-degrading bacteria are important for mucus turnover, stimulating mucus production, and producing beneficial metabolites.
academic.oup.com
January 27, 2026 at 11:26 AM
Another piece of the microbe-mucin interactions puzzle 🧩:
Enzyme extracts of our favourite mucin glycan degraders cleave different bonds that occur in mucins. 🍬
Expression of glycoside hydrolases also differs per species. ✂️
To each their own! 😋
#glycotime #microsky #mucin
doi.org/10.1093/glyc...
Enzyme extracts of our favourite mucin glycan degraders cleave different bonds that occur in mucins. 🍬
Expression of glycoside hydrolases also differs per species. ✂️
To each their own! 😋
#glycotime #microsky #mucin
doi.org/10.1093/glyc...
🧵 New paper from @proteaglyco.bsky.social out in Analytical Chemistry! We used anion supercharging to unlock next-generation O-glycan structural analysis:
- 4× greater signal
- 5× better fragmentation
Here's a thread, digging into each figure:
doi.org/10.1021/acs....
- 4× greater signal
- 5× better fragmentation
Here's a thread, digging into each figure:
doi.org/10.1021/acs....
Anion Supercharging Enables Structural Analysis of Oxidatively Released O-Glycans
O-glycan analysis faces analytical challenges due to poor fragmentation characteristics of oxidatively released O-glycan acids, which can preserve labile O-acetyl modifications but suffer from charge ...
doi.org
April 27, 2026 at 11:51 AM
🧵 New paper from @proteaglyco.bsky.social out in Analytical Chemistry! We used anion supercharging to unlock next-generation O-glycan structural analysis:
- 4× greater signal
- 5× better fragmentation
Here's a thread, digging into each figure:
doi.org/10.1021/acs....
- 4× greater signal
- 5× better fragmentation
Here's a thread, digging into each figure:
doi.org/10.1021/acs....
Can glycans catalyze chemical reactions?
Check out our glycan foldamer that uses carbohydrate–aromatic interactions to perform catalysis, today out in @naturechemistry.bsky.social
Big congrats to Kaimeng!
@mpici.bsky.social
www.nature.com/articles/s41...
Check out our glycan foldamer that uses carbohydrate–aromatic interactions to perform catalysis, today out in @naturechemistry.bsky.social
Big congrats to Kaimeng!
@mpici.bsky.social
www.nature.com/articles/s41...
A glycan foldamer that uses carbohydrate–aromatic interactions to perform catalysis - Nature Chemistry
In nature, catalysis is generally performed by proteins and ribozymes. Now it has been shown that glycans can be designed to perform catalysis. Exploiting carbohydrate–aromatic interactions, a glycan ...
www.nature.com
February 26, 2025 at 10:14 AM
Can glycans catalyze chemical reactions?
Check out our glycan foldamer that uses carbohydrate–aromatic interactions to perform catalysis, today out in @naturechemistry.bsky.social
Big congrats to Kaimeng!
@mpici.bsky.social
www.nature.com/articles/s41...
Check out our glycan foldamer that uses carbohydrate–aromatic interactions to perform catalysis, today out in @naturechemistry.bsky.social
Big congrats to Kaimeng!
@mpici.bsky.social
www.nature.com/articles/s41...
Valerie Lensch of @kiesslinglab.bsky.social and the Johnson group reviewed different glycan-based strategies for eliciting an immune response: academic.oup.com/glycob/artic... #glycotime
Glycoconjugate vaccines: platforms and adjuvants for directed immunity
Abstract. Central to immune recognition is the glycocalyx, a glycan-rich coat on all cells that plays a crucial role in interactions that enable pathogen d
academic.oup.com
December 9, 2024 at 12:27 PM
Valerie Lensch of @kiesslinglab.bsky.social and the Johnson group reviewed different glycan-based strategies for eliciting an immune response: academic.oup.com/glycob/artic... #glycotime
How to study glycosylation using bioorthogonal glycan-based tools: now in one comprehensive review. Highly recommended!
tinyurl.com/27z2rhvv
#glycotime
tinyurl.com/27z2rhvv
#glycotime
September 21, 2026 at 9:07 AM
How to study glycosylation using bioorthogonal glycan-based tools: now in one comprehensive review. Highly recommended!
tinyurl.com/27z2rhvv
#glycotime
tinyurl.com/27z2rhvv
#glycotime