Our paper showing decreased KCC2 expression in the human spinal cord in people who died with a history of neuropathic pain is now published in PAIN: journals.lww.com/pain/abstrac... Great work from @oliviadavis.bsky.social and the teams of the De Koninck and Hildebrand labs. ❤️ 🇨🇦 collabs!
Decreased KCC2 expression in the human spinal dorsal horn... : PAIN
date there is no evidence supporting or opposing this hypothesis in humans. Here, we demonstrate that KCC2 expression is decreased in superficial dorsal horn neurons of organ donors who died with a do...
journals.lww.com
July 22, 2025 at 11:01 PM
Our paper showing decreased KCC2 expression in the human spinal cord in people who died with a history of neuropathic pain is now published in PAIN: journals.lww.com/pain/abstrac... Great work from @oliviadavis.bsky.social and the teams of the De Koninck and Hildebrand labs. ❤️ 🇨🇦 collabs!
Very excited to share our new preprint: "Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use" led by @oliviadavis.bsky.social and Samuel Ferland, a collab between us, Yves De Koninck and Mike Hildebrand labs www.biorxiv.org/content/10.1...
Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use
Loss of GABAergic and glycinergic inhibitory efficacy in the spinal dorsal horn is associated with neuropathic pain and opioid-induced hyperalgesia in rodent models. Downregulation of the KCC2 chlorid...
www.biorxiv.org
January 20, 2025 at 2:05 PM
Very excited to share our new preprint: "Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use" led by @oliviadavis.bsky.social and Samuel Ferland, a collab between us, Yves De Koninck and Mike Hildebrand labs www.biorxiv.org/content/10.1...
#Targetome
CLP290-mediated KCC2 activation restores gait, inhibitory neurotransmission, and metabolic balance across the neuromuscular axis in dystrophic mice.
Details: https://www.maxapress.com/article/doi/10.48130/targetome-0026-0041
#Duchenne #KCC2 #Neuromuscular #Metabolomics
CLP290-mediated KCC2 activation restores gait, inhibitory neurotransmission, and metabolic balance across the neuromuscular axis in dystrophic mice.
Details: https://www.maxapress.com/article/doi/10.48130/targetome-0026-0041
#Duchenne #KCC2 #Neuromuscular #Metabolomics
September 27, 2026 at 4:44 AM
#Targetome
CLP290-mediated KCC2 activation restores gait, inhibitory neurotransmission, and metabolic balance across the neuromuscular axis in dystrophic mice.
Details: https://www.maxapress.com/article/doi/10.48130/targetome-0026-0041
#Duchenne #KCC2 #Neuromuscular #Metabolomics
CLP290-mediated KCC2 activation restores gait, inhibitory neurotransmission, and metabolic balance across the neuromuscular axis in dystrophic mice.
Details: https://www.maxapress.com/article/doi/10.48130/targetome-0026-0041
#Duchenne #KCC2 #Neuromuscular #Metabolomics
7/ The answer lies in neuronal chloride homeostasis. Neurons near HGG cells have elevated intracellular Cl-, partly due to reduced KCC2 expression, making GABAA receptor signaling depolarizing. Indeed, GAA increased neuronal firing in HGG-infiltrated brain, but not in the contralateral hemisphere.
September 24, 2026 at 5:52 PM
7/ The answer lies in neuronal chloride homeostasis. Neurons near HGG cells have elevated intracellular Cl-, partly due to reduced KCC2 expression, making GABAA receptor signaling depolarizing. Indeed, GAA increased neuronal firing in HGG-infiltrated brain, but not in the contralateral hemisphere.
Hi, you probably know me for my doll comp record of 3 comp wins (StarDNTM,KCC2&ETF1) and all finalists.
Here are some of my best looks and I hope to post more doll customs and art here!
#dollsky
Here are some of my best looks and I hope to post more doll customs and art here!
#dollsky
October 29, 2024 at 1:01 PM
Hi, you probably know me for my doll comp record of 3 comp wins (StarDNTM,KCC2&ETF1) and all finalists.
Here are some of my best looks and I hope to post more doll customs and art here!
#dollsky
Here are some of my best looks and I hope to post more doll customs and art here!
#dollsky
Fantastic work here. A real boost to the KCC2 modulator concept, providing very encouraging validation in human subjects. Ted, Yves, Mike and teams!
Very excited to share our new preprint: "Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use" led by @oliviadavis.bsky.social and Samuel Ferland, a collab between us, Yves De Koninck and Mike Hildebrand labs www.biorxiv.org/content/10.1...
Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use
Loss of GABAergic and glycinergic inhibitory efficacy in the spinal dorsal horn is associated with neuropathic pain and opioid-induced hyperalgesia in rodent models. Downregulation of the KCC2 chlorid...
www.biorxiv.org
January 20, 2025 at 5:34 PM
Fantastic work here. A real boost to the KCC2 modulator concept, providing very encouraging validation in human subjects. Ted, Yves, Mike and teams!
Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use https://www.biorxiv.org/content/10.1101/2025.01.19.633733v1
January 20, 2025 at 8:06 AM
Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use https://www.biorxiv.org/content/10.1101/2025.01.19.633733v1
Amazing new paper out on KCC2 expression in human spinal cord! Love the translational potential of these findings. Seems like chloride dysfunction could be really important in chronic pain! Great work @tedpricethepainguy.bsky.social, Mike, Yves, and the team!
#Science
#Science
Our paper showing decreased KCC2 expression in the human spinal cord in people who died with a history of neuropathic pain is now published in PAIN: journals.lww.com/pain/abstrac... Great work from @oliviadavis.bsky.social and the teams of the De Koninck and Hildebrand labs. ❤️ 🇨🇦 collabs!
Decreased KCC2 expression in the human spinal dorsal horn... : PAIN
date there is no evidence supporting or opposing this hypothesis in humans. Here, we demonstrate that KCC2 expression is decreased in superficial dorsal horn neurons of organ donors who died with a do...
journals.lww.com
July 26, 2025 at 2:46 AM
Amazing new paper out on KCC2 expression in human spinal cord! Love the translational potential of these findings. Seems like chloride dysfunction could be really important in chronic pain! Great work @tedpricethepainguy.bsky.social, Mike, Yves, and the team!
#Science
#Science
Did you see Davis et al.’s data in @painthejournal.bsky.social replicating rodent data showing decreased expression of a potassium and chloride ion cotransporter, KCC2, in human superficial dorsal horn neurons? Check out that finding and more here bit.ly/4f7NhXp #PRF #EditorsPick
August 1, 2025 at 2:00 PM
Did you see Davis et al.’s data in @painthejournal.bsky.social replicating rodent data showing decreased expression of a potassium and chloride ion cotransporter, KCC2, in human superficial dorsal horn neurons? Check out that finding and more here bit.ly/4f7NhXp #PRF #EditorsPick
The magnitude of the change in KCC2 protein is massive. We used a very conservative measure of membrane localization and did not quantify overall protein but judge for yourself in the images we provide. The data strongly supports KCC2 positive modulation for pain.
July 22, 2025 at 11:03 PM
The magnitude of the change in KCC2 protein is massive. We used a very conservative measure of membrane localization and did not quantify overall protein but judge for yourself in the images we provide. The data strongly supports KCC2 positive modulation for pain.
Activation of KCC2 during development alleviates cognitive, behavioral, and neural excitability in adult CDKL5-deficient mice https://www.biorxiv.org/content/10.1101/2025.02.26.640365v1
February 27, 2025 at 8:15 AM
Activation of KCC2 during development alleviates cognitive, behavioral, and neural excitability in adult CDKL5-deficient mice https://www.biorxiv.org/content/10.1101/2025.02.26.640365v1
This is a big moment for the KCC2 story. Congrats to you, Olivia, and Sam, as well as the teams with Mike and Yves!
Very excited to share our new preprint: "Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use" led by @oliviadavis.bsky.social and Samuel Ferland, a collab between us, Yves De Koninck and Mike Hildebrand labs www.biorxiv.org/content/10.1...
Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use
Loss of GABAergic and glycinergic inhibitory efficacy in the spinal dorsal horn is associated with neuropathic pain and opioid-induced hyperalgesia in rodent models. Downregulation of the KCC2 chlorid...
www.biorxiv.org
January 21, 2025 at 1:12 AM
This is a big moment for the KCC2 story. Congrats to you, Olivia, and Sam, as well as the teams with Mike and Yves!
Overall, very exciting times ahead for KCC2 as a therapeutic target. To my knowledge, this is the first, or one of the first, validations of a pain target in human spinal cord. Thanks to the organ donors and their families for their gift that makes this exciting work possible!
January 20, 2025 at 2:26 PM
Overall, very exciting times ahead for KCC2 as a therapeutic target. To my knowledge, this is the first, or one of the first, validations of a pain target in human spinal cord. Thanks to the organ donors and their families for their gift that makes this exciting work possible!
🚨 New preprint from the lab!
🧠 Despite representing <5% of total #KCC2 in mature neurons, we show that the KCC2a isoform acts as a powerful developmental switch: inhibiting KCC2b early on, then boosting its membrane expression and fonction in the adult brain.
www.biorxiv.org/content/10.6...
🧠 Despite representing <5% of total #KCC2 in mature neurons, we show that the KCC2a isoform acts as a powerful developmental switch: inhibiting KCC2b early on, then boosting its membrane expression and fonction in the adult brain.
www.biorxiv.org/content/10.6...
www.biorxiv.org
January 29, 2026 at 2:59 PM
🚨 New preprint from the lab!
🧠 Despite representing <5% of total #KCC2 in mature neurons, we show that the KCC2a isoform acts as a powerful developmental switch: inhibiting KCC2b early on, then boosting its membrane expression and fonction in the adult brain.
www.biorxiv.org/content/10.6...
🧠 Despite representing <5% of total #KCC2 in mature neurons, we show that the KCC2a isoform acts as a powerful developmental switch: inhibiting KCC2b early on, then boosting its membrane expression and fonction in the adult brain.
www.biorxiv.org/content/10.6...
🚨 New Insights into Chronic Pain 🚨
Reduced KCC2 expression in the dorsal horn links to chronic pain and opioid use. KCC2 regulates chloride gradients for inhibition, and its dysfunction drives neuropathic pain. Findings support therapies targeting KCC2.
Read more: www.biorxiv.org/content/10.1...
Reduced KCC2 expression in the dorsal horn links to chronic pain and opioid use. KCC2 regulates chloride gradients for inhibition, and its dysfunction drives neuropathic pain. Findings support therapies targeting KCC2.
Read more: www.biorxiv.org/content/10.1...
Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use
Loss of GABAergic and glycinergic inhibitory efficacy in the spinal dorsal horn is associated with neuropathic pain and opioid-induced hyperalgesia in rodent models. Downregulation of the KCC2 chlorid...
www.biorxiv.org
February 28, 2025 at 1:54 PM
🚨 New Insights into Chronic Pain 🚨
Reduced KCC2 expression in the dorsal horn links to chronic pain and opioid use. KCC2 regulates chloride gradients for inhibition, and its dysfunction drives neuropathic pain. Findings support therapies targeting KCC2.
Read more: www.biorxiv.org/content/10.1...
Reduced KCC2 expression in the dorsal horn links to chronic pain and opioid use. KCC2 regulates chloride gradients for inhibition, and its dysfunction drives neuropathic pain. Findings support therapies targeting KCC2.
Read more: www.biorxiv.org/content/10.1...
www.youtube.com/watch?v=KcC2...
一年前から尾行されていますって言うから恨み…？ストーカー？と思ってたけど頼まれたらサインするもんだから中国人転売ヤー組織(日本人も）が外出すると延々つきまとってサインをねだってくる(高いカードとかに）らしい。個人相手にそんなことになんの！？
一年前から尾行されていますって言うから恨み…？ストーカー？と思ってたけど頼まれたらサインするもんだから中国人転売ヤー組織(日本人も）が外出すると延々つきまとってサインをねだってくる(高いカードとかに）らしい。個人相手にそんなことになんの！？
【閲覧注意】尾行・つきまといをされ、サイン中止せざるをえなくなりました。
👉【弁護士に聞く】この絵、トレパクですか！？https://youtu.be/bOjgkuT7i5g—————🍀1ヶ月で絵が上手くなる動画🍀———————👉全巻セットhttps://vimeo.com/ondemand/naokisaito👉単品購入/レンタルhttps://vimeo.com/on...
www.youtube.com
March 17, 2024 at 5:34 AM
www.youtube.com/watch?v=KcC2...
一年前から尾行されていますって言うから恨み…？ストーカー？と思ってたけど頼まれたらサインするもんだから中国人転売ヤー組織(日本人も）が外出すると延々つきまとってサインをねだってくる(高いカードとかに）らしい。個人相手にそんなことになんの！？
一年前から尾行されていますって言うから恨み…？ストーカー？と思ってたけど頼まれたらサインするもんだから中国人転売ヤー組織(日本人も）が外出すると延々つきまとってサインをねだってくる(高いカードとかに）らしい。個人相手にそんなことになんの！？
The authors included RRIDs in their in bioRxiv paper! We value the author's support of reproducibility. #reproducibility #BetterScience #methodsmatter
Ganglioside GM1-enriched rafts regulate the neuronal chloride co-transporter KCC2
Read the full paper: Ganglioside GM1-enriched rafts regulate the neuronal chloride co-transporter KCC2
doi.org
April 2, 2026 at 7:03 AM
The authors included RRIDs in their in bioRxiv paper! We value the author's support of reproducibility. #reproducibility #BetterScience #methodsmatter
Also want to point out this great paper from @annatheneurosci.bsky.social and Cathy Cahill that inspired the work on opioid consumption and KCC2 in our paper: pubmed.ncbi.nlm.nih.gov/26202104/
Neuroimmune Regulation of GABAergic Neurons Within the Ventral Tegmental Area During Withdrawal from Chronic Morphine - PubMed
Opioid dependence is accompanied by neuroplastic changes in reward circuitry leading to a negative affective state contributing to addictive behaviors and risk of relapse. The current study presents a...
pubmed.ncbi.nlm.nih.gov
July 22, 2025 at 11:07 PM
Also want to point out this great paper from @annatheneurosci.bsky.social and Cathy Cahill that inspired the work on opioid consumption and KCC2 in our paper: pubmed.ncbi.nlm.nih.gov/26202104/
Ganglioside GM1-enriched rafts regulate the neuronal chloride co-transporter 1 KCC2. https://www.biorxiv.org/content/10.64898/2026.03.22.713396v1
March 25, 2026 at 1:15 AM
Ganglioside GM1-enriched rafts regulate the neuronal chloride co-transporter 1 KCC2. https://www.biorxiv.org/content/10.64898/2026.03.22.713396v1
Hyperreflexia after corticospinal tract lesion reflects 1A afferent circuit changes not increased KCC2 hyperexcitability https://www.biorxiv.org/content/10.1101/2025.02.21.639555v1
February 23, 2025 at 8:15 AM
Hyperreflexia after corticospinal tract lesion reflects 1A afferent circuit changes not increased KCC2 hyperexcitability https://www.biorxiv.org/content/10.1101/2025.02.21.639555v1
In our (Dallas) cohort we found KCC2 membrane downregulation in donors with a history of chronic pain, and in a separate cohort of individuals with a history or opioid use disorder. Very clear evidence of decreased KCC2 function in both of these disorders.
January 20, 2025 at 2:21 PM
In our (Dallas) cohort we found KCC2 membrane downregulation in donors with a history of chronic pain, and in a separate cohort of individuals with a history or opioid use disorder. Very clear evidence of decreased KCC2 function in both of these disorders.
TGF-beta2 regulates neuronal Ankyrin-G and promotes its interaction with KCC2 https://www.biorxiv.org/content/10.1101/2023.12.05.570078v1
TGF-beta2 regulates neuronal Ankyrin-G and promotes its interaction with KCC2 https://www.biorxiv.org/content/10.1101/2023.12.05.570078v1
The neuronal K+/Cl- cotransporter 2 (KCC2) is the major Cl- extruder in CNS neurons and responsible
www.biorxiv.org
December 7, 2023 at 8:16 AM
TGF-beta2 regulates neuronal Ankyrin-G and promotes its interaction with KCC2 https://www.biorxiv.org/content/10.1101/2023.12.05.570078v1
Hyperreflexia after corticospinal tract lesion reflects 1A afferent circuit changes not increased KCC2 hyperexcitability https://www.biorxiv.org/content/10.1101/2025.02.21.639555v1
February 23, 2025 at 8:15 AM
Hyperreflexia after corticospinal tract lesion reflects 1A afferent circuit changes not increased KCC2 hyperexcitability https://www.biorxiv.org/content/10.1101/2025.02.21.639555v1
Motion‐Style Scalp Acupuncture Ameliorates Post‐Stroke Muscle Spasticity Through Cerebral Blood Flow Augmentation and 5‐HT2AR‐Mediated Spinal KCC2 Reactivation Br&Beh
Motion‐Style Scalp Acupuncture Ameliorates Post‐Stroke Muscle Spasticity Through Cerebral Blood Flow Augmentation and 5‐HT2AR‐Mediated Spinal KCC2 Reactivation
Brain and Behavior, Volume 15, Issue 12, December 2025.
dlvr.it
December 6, 2025 at 7:15 AM
Motion‐Style Scalp Acupuncture Ameliorates Post‐Stroke Muscle Spasticity Through Cerebral Blood Flow Augmentation and 5‐HT2AR‐Mediated Spinal KCC2 Reactivation Br&Beh