Silly AU shenanigans with Captain Hook Vox finally capturing the elusive merfish Alastor. I’m sure he has a fish bowl waiting just for him on his ship.
#radiostatic #Hazbinhotel #Hazbinhotelvox #Hazbinhotelalastor
#radiostatic #Hazbinhotel #Hazbinhotelvox #Hazbinhotelalastor
February 1, 2026 at 11:35 PM
Silly AU shenanigans with Captain Hook Vox finally capturing the elusive merfish Alastor. I’m sure he has a fish bowl waiting just for him on his ship.
#radiostatic #Hazbinhotel #Hazbinhotelvox #Hazbinhotelalastor
#radiostatic #Hazbinhotel #Hazbinhotelvox #Hazbinhotelalastor
Be still my scientific ❤️.... @biorxivpreprint.bsky.social high resolution spatial transcriptomics on human meninges
🤩 work from Kevin Petrecca group at McGill University led by Theresa Degenhard - www.biorxiv.org/content/10.1... 🧪 #Neuroscience
🤩 work from Kevin Petrecca group at McGill University led by Theresa Degenhard - www.biorxiv.org/content/10.1... 🧪 #Neuroscience
November 19, 2024 at 3:33 PM
Be still my scientific ❤️.... @biorxivpreprint.bsky.social high resolution spatial transcriptomics on human meninges
🤩 work from Kevin Petrecca group at McGill University led by Theresa Degenhard - www.biorxiv.org/content/10.1... 🧪 #Neuroscience
🤩 work from Kevin Petrecca group at McGill University led by Theresa Degenhard - www.biorxiv.org/content/10.1... 🧪 #Neuroscience
In bits of personal glee from the weekend:
- Con leafing through the (yes, I know, very late) mup calendar and laughing himself silly at the merfish and December pages
- also asking of Ewen Bremner had seen the Buttons page and immediately taking a picture of it to send him
#ourflagmeansmuppets
- Con leafing through the (yes, I know, very late) mup calendar and laughing himself silly at the merfish and December pages
- also asking of Ewen Bremner had seen the Buttons page and immediately taking a picture of it to send him
#ourflagmeansmuppets
June 23, 2025 at 7:32 PM
In bits of personal glee from the weekend:
- Con leafing through the (yes, I know, very late) mup calendar and laughing himself silly at the merfish and December pages
- also asking of Ewen Bremner had seen the Buttons page and immediately taking a picture of it to send him
#ourflagmeansmuppets
- Con leafing through the (yes, I know, very late) mup calendar and laughing himself silly at the merfish and December pages
- also asking of Ewen Bremner had seen the Buttons page and immediately taking a picture of it to send him
#ourflagmeansmuppets
Proud to announce our new technique for tracking relative B cell clonality in situ with B Cell Receptor MERFISH (BCR-MERFISH)! Congratulations to Evan Yang and our colleagues in the Carroll laboratory! Check out our bioRxiv: www.biorxiv.org/content/10.1... 1/9
May 28, 2025 at 3:40 PM
Proud to announce our new technique for tracking relative B cell clonality in situ with B Cell Receptor MERFISH (BCR-MERFISH)! Congratulations to Evan Yang and our colleagues in the Carroll laboratory! Check out our bioRxiv: www.biorxiv.org/content/10.1... 1/9
March 24, 2026 at 9:16 AM
Let's try something here,
THUMBNAIL ADOPTABLES
15 BUCKS A PIECE
COMES WITH THUMBNAIL REFERENCE PAGE
DM OR REPLY TO CLAIM
THUMBNAIL ADOPTABLES
15 BUCKS A PIECE
COMES WITH THUMBNAIL REFERENCE PAGE
DM OR REPLY TO CLAIM
July 16, 2025 at 1:57 AM
Let's try something here,
THUMBNAIL ADOPTABLES
15 BUCKS A PIECE
COMES WITH THUMBNAIL REFERENCE PAGE
DM OR REPLY TO CLAIM
THUMBNAIL ADOPTABLES
15 BUCKS A PIECE
COMES WITH THUMBNAIL REFERENCE PAGE
DM OR REPLY TO CLAIM
With this “Behind the paper” story, I’d like to give special thanks to two remarkable contributors whose generosity made a big difference:
@jialinliu.bsky.social for the scMultiomics data behind the multiomic atlas,
and Guoqiang Yu’s group for sharing ITEC cell tracking, which enabled MERFISH-FATE.
@jialinliu.bsky.social for the scMultiomics data behind the multiomic atlas,
and Guoqiang Yu’s group for sharing ITEC cell tracking, which enabled MERFISH-FATE.
From fate to form: the journey to whole-embryo spatial transcriptomics
In this 'Behind the paper' feature, @yinanwan.bsky.social shares the inside story of an ambitious journey with @schierlab.bsky.social & co.
thenode.biologists.com/from-fate-to...
In this 'Behind the paper' feature, @yinanwan.bsky.social shares the inside story of an ambitious journey with @schierlab.bsky.social & co.
thenode.biologists.com/from-fate-to...
From fate to form: the journey to whole-embryo spatial transcriptomics - the Node
Where molecules meet form I have long been interested in understanding how development emerges at the intersection of molecular and spatial organization.
thenode.biologists.com
April 2, 2026 at 4:02 PM
With this “Behind the paper” story, I’d like to give special thanks to two remarkable contributors whose generosity made a big difference:
@jialinliu.bsky.social for the scMultiomics data behind the multiomic atlas,
and Guoqiang Yu’s group for sharing ITEC cell tracking, which enabled MERFISH-FATE.
@jialinliu.bsky.social for the scMultiomics data behind the multiomic atlas,
and Guoqiang Yu’s group for sharing ITEC cell tracking, which enabled MERFISH-FATE.
Whole-body Single-cell Spatial transcriptomic atlas of adult Danionella ~1 cm long
scRNAseq
MERFISH (500-gene)
HCR
Endothelial cell podxl+ (Fig S8)
Vascular vs Visceral mural cell (Fig S10)
Peter Reddien lab bioRxiv 2026
www.biorxiv.org/content/10.6...
scRNAseq
MERFISH (500-gene)
HCR
Endothelial cell podxl+ (Fig S8)
Vascular vs Visceral mural cell (Fig S10)
Peter Reddien lab bioRxiv 2026
www.biorxiv.org/content/10.6...
February 10, 2026 at 12:57 PM
Whole-body Single-cell Spatial transcriptomic atlas of adult Danionella ~1 cm long
scRNAseq
MERFISH (500-gene)
HCR
Endothelial cell podxl+ (Fig S8)
Vascular vs Visceral mural cell (Fig S10)
Peter Reddien lab bioRxiv 2026
www.biorxiv.org/content/10.6...
scRNAseq
MERFISH (500-gene)
HCR
Endothelial cell podxl+ (Fig S8)
Vascular vs Visceral mural cell (Fig S10)
Peter Reddien lab bioRxiv 2026
www.biorxiv.org/content/10.6...
Jeffrey Moffitt gives an amazing talk on MERFISH! #microbe2026
June 6, 2026 at 9:09 PM
Jeffrey Moffitt gives an amazing talk on MERFISH! #microbe2026
Comparing Imaging-based #SpatialTranscriptimics methods
CosMx vs MERFISH vs Xenium
1k vs 500 vs 339 plex
FFPE👤Lung/Mesothelium Tumor Microarray
CosMX▶️highest transcript counts/cell
Xenium▶️lowest FDR & highest cell segmentation precision
bioRxiv 2024
www.biorxiv.org/content/10.1...
CosMx vs MERFISH vs Xenium
1k vs 500 vs 339 plex
FFPE👤Lung/Mesothelium Tumor Microarray
CosMX▶️highest transcript counts/cell
Xenium▶️lowest FDR & highest cell segmentation precision
bioRxiv 2024
www.biorxiv.org/content/10.1...
December 19, 2024 at 12:21 PM
Comparing Imaging-based #SpatialTranscriptimics methods
CosMx vs MERFISH vs Xenium
1k vs 500 vs 339 plex
FFPE👤Lung/Mesothelium Tumor Microarray
CosMX▶️highest transcript counts/cell
Xenium▶️lowest FDR & highest cell segmentation precision
bioRxiv 2024
www.biorxiv.org/content/10.1...
CosMx vs MERFISH vs Xenium
1k vs 500 vs 339 plex
FFPE👤Lung/Mesothelium Tumor Microarray
CosMX▶️highest transcript counts/cell
Xenium▶️lowest FDR & highest cell segmentation precision
bioRxiv 2024
www.biorxiv.org/content/10.1...
Comparing single-cell #SpatialTranscriptomics methods on FFPE human tumor #TissueMicroArray
CosMx 1k vs MERFISH 500 vs Xenium-UM/-MM 339
93 common genes
lung adenocarcinoma
pleural mesothelioma
Corr. Bulk RNAseq, GeoMx
#NatureComms 2025
www.nature.com/articles/s41...
CosMx 1k vs MERFISH 500 vs Xenium-UM/-MM 339
93 common genes
lung adenocarcinoma
pleural mesothelioma
Corr. Bulk RNAseq, GeoMx
#NatureComms 2025
www.nature.com/articles/s41...
September 28, 2025 at 12:22 PM
Comparing single-cell #SpatialTranscriptomics methods on FFPE human tumor #TissueMicroArray
CosMx 1k vs MERFISH 500 vs Xenium-UM/-MM 339
93 common genes
lung adenocarcinoma
pleural mesothelioma
Corr. Bulk RNAseq, GeoMx
#NatureComms 2025
www.nature.com/articles/s41...
CosMx 1k vs MERFISH 500 vs Xenium-UM/-MM 339
93 common genes
lung adenocarcinoma
pleural mesothelioma
Corr. Bulk RNAseq, GeoMx
#NatureComms 2025
www.nature.com/articles/s41...
Need to design your next Xenium/CosMx/Merfish experiment? Spapros, now out @naturemethods, allows for probe set selection for target spatial tx. Led by @KuemmerleLouis, it optimizes both cell type ID & within-cell type variation. nature.com/articles/s41592-024-02496-z github.com/theislab/spapros
November 20, 2024 at 7:50 AM
Need to design your next Xenium/CosMx/Merfish experiment? Spapros, now out @naturemethods, allows for probe set selection for target spatial tx. Led by @KuemmerleLouis, it optimizes both cell type ID & within-cell type variation. nature.com/articles/s41592-024-02496-z github.com/theislab/spapros
Spatial transcriptomics reveals human cortical layer and area specification rdcu.be/emqcb
Spatial transcriptomics reveals human cortical layer and area specification
Nature - Multiplexed error-robust fluorescence in situ hybridization (MERFISH) together with deep-learning-based nucleus segmentation enabled the construction of a highly detailed and informative...
rdcu.be
May 17, 2025 at 6:31 AM
Spatial transcriptomics reveals human cortical layer and area specification rdcu.be/emqcb
"we developed Multiplexed Error Robust Fluorescence In Situ Hybridization 2.0 (MERFISH 2.0), an optimized spatial transcriptomic imaging chemistry to enhance profiling of fragmented and highly crosslinked RNA"
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
March 9, 2026 at 5:27 PM
"we developed Multiplexed Error Robust Fluorescence In Situ Hybridization 2.0 (MERFISH 2.0), an optimized spatial transcriptomic imaging chemistry to enhance profiling of fragmented and highly crosslinked RNA"
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
A new study, led by Kushal (Berkeley), Nicole&Victoria (UCSF) in full collaboration with @karthikshekhar.bsky.social, "Molecular and spatial analysis of ganglion cells on retinal flatmounts identifies perivascular neurons resilient to glaucoma" sciencedirect.com/science/arti... #retina #MERFISH
August 20, 2025 at 4:06 PM
A new study, led by Kushal (Berkeley), Nicole&Victoria (UCSF) in full collaboration with @karthikshekhar.bsky.social, "Molecular and spatial analysis of ganglion cells on retinal flatmounts identifies perivascular neurons resilient to glaucoma" sciencedirect.com/science/arti... #retina #MERFISH
Really cool #singlecell method published in Science! Multiplexed error-robust fluorescence in situ hybridization (MERFISH) to create an image-based single-cell transcriptomics in bacteria 🦠🧫🔬🧪
🅲🅾🅾🅻 🆃🅴🅲🅷🅽🅾🅻🅾🅶🆈
“Bacterial-MERFISH” provides ~1000-fold volumetric expansion of individual cells, charts gene expression in hundreds of thousands of cells, deciphering bacterial single-cell heterogeneity, intracellular transcriptome organization, and bacterial adaptation to µm-scale niches in vivo
“Bacterial-MERFISH” provides ~1000-fold volumetric expansion of individual cells, charts gene expression in hundreds of thousands of cells, deciphering bacterial single-cell heterogeneity, intracellular transcriptome organization, and bacterial adaptation to µm-scale niches in vivo
Highly multiplexed spatial transcriptomics in bacteria
Single-cell decisions made in complex environments underlie many bacterial phenomena. Image-based transcriptomics approaches offer an avenue to study such behaviors, yet these approaches have been hin...
www.science.org
January 25, 2025 at 5:58 PM
Really cool #singlecell method published in Science! Multiplexed error-robust fluorescence in situ hybridization (MERFISH) to create an image-based single-cell transcriptomics in bacteria 🦠🧫🔬🧪
New In Brief out in The Plant Cell, co-authored with Dr. Travis Lee! We highlight recent work by Katie Long, Ashleigh Lister, and colleagues, who adapt MERFISH–based spatial transcriptomics to the anatomically complex wheat spike. 🧵👇
December 27, 2025 at 9:00 PM
New In Brief out in The Plant Cell, co-authored with Dr. Travis Lee! We highlight recent work by Katie Long, Ashleigh Lister, and colleagues, who adapt MERFISH–based spatial transcriptomics to the anatomically complex wheat spike. 🧵👇
I was wondering what my first bluesky post would be and I thought since it’s Valentine’s Day I’d wish everyone a happy Valentine’s Day with this merfish image of the axolotl telencephalon. ❤️
February 14, 2024 at 6:35 PM
I was wondering what my first bluesky post would be and I thought since it’s Valentine’s Day I’d wish everyone a happy Valentine’s Day with this merfish image of the axolotl telencephalon. ❤️
Abyssal merfish Alastor AU who saw the sun for the first and last time #radioapple
February 17, 2025 at 6:38 AM
Abyssal merfish Alastor AU who saw the sun for the first and last time #radioapple
19. Subcellular and tissue-level spatial resolution of transcriptomes is also possible. MERFISH is one popular technique, explained in this video: www.youtube.com/watch?v=MoDF...
Using MERFISH for Spatially Resolved Transcriptomics
YouTube video by GH
www.youtube.com
March 23, 2025 at 8:46 PM
19. Subcellular and tissue-level spatial resolution of transcriptomes is also possible. MERFISH is one popular technique, explained in this video: www.youtube.com/watch?v=MoDF...
MERFISH used to create a spatial atlas of healthy human skin @natgenet.nature.com
www.nature.com/articles/s41...
@paularstrpo.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
@paularstrpo.bsky.social
www.nature.com/articles/s41...
A refined blueprint for human skin - Nature Genetics
Bu using organ-wide single-cell MERFISH, researchers create a spatial atlas of healthy human skin and identify distinct site-specific cellular compositions. This work emphasize the importance of skin ...
www.nature.com
March 24, 2026 at 8:27 PM
MERFISH used to create a spatial atlas of healthy human skin @natgenet.nature.com
www.nature.com/articles/s41...
@paularstrpo.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
@paularstrpo.bsky.social
www.nature.com/articles/s41...
🅲🅾🅾🅻 🆃🅴🅲🅷🅽🅾🅻🅾🅶🆈
“Bacterial-MERFISH” provides ~1000-fold volumetric expansion of individual cells, charts gene expression in hundreds of thousands of cells, deciphering bacterial single-cell heterogeneity, intracellular transcriptome organization, and bacterial adaptation to µm-scale niches in vivo
“Bacterial-MERFISH” provides ~1000-fold volumetric expansion of individual cells, charts gene expression in hundreds of thousands of cells, deciphering bacterial single-cell heterogeneity, intracellular transcriptome organization, and bacterial adaptation to µm-scale niches in vivo
Highly multiplexed spatial transcriptomics in bacteria
Single-cell decisions made in complex environments underlie many bacterial phenomena. Image-based transcriptomics approaches offer an avenue to study such behaviors, yet these approaches have been hin...
www.science.org
January 25, 2025 at 5:33 PM
🅲🅾🅾🅻 🆃🅴🅲🅷🅽🅾🅻🅾🅶🆈
“Bacterial-MERFISH” provides ~1000-fold volumetric expansion of individual cells, charts gene expression in hundreds of thousands of cells, deciphering bacterial single-cell heterogeneity, intracellular transcriptome organization, and bacterial adaptation to µm-scale niches in vivo
“Bacterial-MERFISH” provides ~1000-fold volumetric expansion of individual cells, charts gene expression in hundreds of thousands of cells, deciphering bacterial single-cell heterogeneity, intracellular transcriptome organization, and bacterial adaptation to µm-scale niches in vivo
GASTON
Gradient Analysis of #Spatialtranscriptomics Organization with Neural networks
"models gene expression as a Piecewise linear function of the Isodepth"
Work with Visium Xenium MERFISH Slide-seq V2 Stereo-seq
@uthsav.bsky.social @naturemethods.bsky.social 2025
www.nature.com/articles/s41...
Gradient Analysis of #Spatialtranscriptomics Organization with Neural networks
"models gene expression as a Piecewise linear function of the Isodepth"
Work with Visium Xenium MERFISH Slide-seq V2 Stereo-seq
@uthsav.bsky.social @naturemethods.bsky.social 2025
www.nature.com/articles/s41...
March 6, 2025 at 12:23 PM
GASTON
Gradient Analysis of #Spatialtranscriptomics Organization with Neural networks
"models gene expression as a Piecewise linear function of the Isodepth"
Work with Visium Xenium MERFISH Slide-seq V2 Stereo-seq
@uthsav.bsky.social @naturemethods.bsky.social 2025
www.nature.com/articles/s41...
Gradient Analysis of #Spatialtranscriptomics Organization with Neural networks
"models gene expression as a Piecewise linear function of the Isodepth"
Work with Visium Xenium MERFISH Slide-seq V2 Stereo-seq
@uthsav.bsky.social @naturemethods.bsky.social 2025
www.nature.com/articles/s41...