YouTube video by New Riders of the Purple Sage - Topic

YouTube video by New Riders Of The Purple Sage - Topic

Our first goal was to discover if there is structural variation among GXP. To do this, we first needed to define what counts as a GxpS based on common descent and orthology of their synthetases. This task is complicated by the complex and hierarchical histories of NRPS: whole-NRPS genes undergo duplication events, gene fusions and/or horizontal transfer between bacteria25. In addition, individual domains of NRPS also duplicate to give rise to different kinds of A- and C-domains. Finally, modules can also recombine between different NRPS. Each of these processes introduces different phylogenetic incongruences between the species these genes evolve in and the genes themselves. To untangle this complex hierarchy, we used the TE-domain as our phylogenetic yardstick (Fig. 1c and Extended Data Fig. 1a). TE-domains usually occur just once in NRPS. Further, recombination-aware methods could not identify any recombinations in TE-domains from GxpS. We can therefore use the TE-domain in analogy to a conventional gene tree, which reports on whole-NRPS duplications and transfers, but is unaffected by individual domain duplications and recombinations. We inferred a tree of TTE-domains (T5- and TE-domains) of 54 putative GxpS orthologues (Fig. 1c). We added the T-domain to the TE-domain because it added more sequence...

YouTube video by Scott Free

YouTube video by The Kind Electric Burrito

Elucidating the biological origins and biosynthetic pathways of marine natural products remains a major challenge, particularly for compounds deriv...

Ontario’s Special Investigations Unit (SIU) is investigating the death of a 40-year-old man in hospital on October 1, 2026, following his arrest by Niagara Regional Police Service (NRPS) officers o…

Ontario’s Special Investigations Unit (SIU) is investigating the death of a 40-year-old man in hospital on October 1, 2026, following his arrest by Niagara Regional Police Service (NRPS) officers o…

YouTube video by Phil Matera

YouTube video by musicstuffstpete

AbstractWe report the genome-guided discovery, isolation, and characterization of floridanemastatin (1), a previously undescribed statine-containing polyketide−peptide from Floridanema evergladense BLCC-F167. Examination of the biosynthetic gene clusters in the F.evergladense genome revealed a hybrid PKS−NRPS biosynthetic gene cluster featuring an NRPS module followed by a PKS ketoextension and ketoreductase domain arrangement diagnostic of statine biosynthesis. Guided by this prediction, LC−MS-based analysis of cultured biomass led to the isolation of floridanemastatin. High-resolution mass spectrometry and comprehensive 1D and 2D NMR spectroscopy defined a polyketide−peptide scaffold incorporating serine, proline, a leucine-derived amino alcohol, and a statine-like residue identified as 4-amino-3-hydroxy-(4-hydroxyphenyl)pentanoic acid. Connectivity and residue assignments were consistent with the predicted biosynthetic logic of the gene cluster, including reductive chain termination to yield an alcohol. Although the statine pharmacophore and in silico target prediction suggested cathepsin D as a plausible target, enzymatic evaluation indicated that apparent inhibition was attributable to nonspecific aggregation under the assay conditions. These findings demonstrate the utility of biosynthetic gene cluster architecture for prospecting statine-containing cyanobacterial natural products and further highlights the genus Floridanema as a source of chemically distinctive specialized metabolites.

YouTube video by New Riders Of The Purple Sage - Topic