Antibodies don't work.
NLRP3 is by far the most studied inflammasome — yet <30% of commercial antibodies were specific.
Only 4 of 14 tested antibodies detected NLRP3 specifically.
Antibodies must be validated before use.
Full report by @ycharos.bsky.social : zenodo.org/records/1162...
NLRP3 is by far the most studied inflammasome — yet <30% of commercial antibodies were specific.
Only 4 of 14 tested antibodies detected NLRP3 specifically.
Antibodies must be validated before use.
Full report by @ycharos.bsky.social : zenodo.org/records/1162...
April 30, 2025 at 6:15 AM
Antibodies don't work.
NLRP3 is by far the most studied inflammasome — yet <30% of commercial antibodies were specific.
Only 4 of 14 tested antibodies detected NLRP3 specifically.
Antibodies must be validated before use.
Full report by @ycharos.bsky.social : zenodo.org/records/1162...
NLRP3 is by far the most studied inflammasome — yet <30% of commercial antibodies were specific.
Only 4 of 14 tested antibodies detected NLRP3 specifically.
Antibodies must be validated before use.
Full report by @ycharos.bsky.social : zenodo.org/records/1162...
I am thrilled to share our latest article that has been published in @cp-immunity.bsky.social. We describe a new role for NLRP3 outside of its #inflammasome complex, where it can regulate microglial #metabolism & key metabolites that affect the epigenetic landscape(1)
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
NLRP3-mediated glutaminolysis controls microglial phagocytosis to promote Alzheimer’s disease progression
How NLRP3 impacts Alzheimer’s disease (AD) is unclear. McManus et al. find that NLRP3
is located at mitochondria where it regulates microglial metabolism. Depletion or
chronic pharmacological inhibiti...
www.cell.com
February 3, 2025 at 4:18 PM
I am thrilled to share our latest article that has been published in @cp-immunity.bsky.social. We describe a new role for NLRP3 outside of its #inflammasome complex, where it can regulate microglial #metabolism & key metabolites that affect the epigenetic landscape(1)
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
An NLRP3 stimulatory universal influenza vaccine.
We report an NLRP3 stimulus that generates hyperactive dendritic cells (hDCs).
journals.asm.org/doi/10.1128/...
We report an NLRP3 stimulus that generates hyperactive dendritic cells (hDCs).
journals.asm.org/doi/10.1128/...
An NLRP3-stimulatory adjuvant improves the immunogenicity of influenza virus vaccines in mice and non-human primates | mBio
The generation of vaccines that stimulate T cell activities is an unmet need for the
scientific community, as T cells are the primary mediators of immune memory. In this
study, we report a new vaccine...
journals.asm.org
September 8, 2025 at 7:19 PM
An NLRP3 stimulatory universal influenza vaccine.
We report an NLRP3 stimulus that generates hyperactive dendritic cells (hDCs).
journals.asm.org/doi/10.1128/...
We report an NLRP3 stimulus that generates hyperactive dendritic cells (hDCs).
journals.asm.org/doi/10.1128/...
#InflammasomePower! 💥New paper alert💥 How is NLPR3 primed & activated in chronic non-communicable inflammatory diseases? With @oceanedufies.bsky.social &co we show that chronic exposure to oxPAPC drives NRF2 activation that primes & activates NLRP3 sustaining #atherosclerosis in mice & humans! 👇👇👇
Chronic sensing of host-derived lipids is an all-in-one signal that primes and activates NLRP3.
Activation of the NLRP3 inflammasome leads to the production of bioactive interleukin (IL)-1β fostering atherosclerosis. The current dogma is that NLRP3 must be first primed by microbial stimuli, know...
www.biorxiv.org
October 31, 2025 at 3:49 PM
#InflammasomePower! 💥New paper alert💥 How is NLPR3 primed & activated in chronic non-communicable inflammatory diseases? With @oceanedufies.bsky.social &co we show that chronic exposure to oxPAPC drives NRF2 activation that primes & activates NLRP3 sustaining #atherosclerosis in mice & humans! 👇👇👇
NLRP3-mediated glutaminolysis controls microglial phagocytosis to promote Alzheimer’s disease progression
www.cell.com/immunity/ful...
@cp-immunity.bsky.social @roisinmcmanus.bsky.social
www.cell.com/immunity/ful...
@cp-immunity.bsky.social @roisinmcmanus.bsky.social
NLRP3-mediated glutaminolysis controls microglial phagocytosis to promote Alzheimer’s disease progression
How NLRP3 impacts Alzheimer’s disease (AD) is unclear. McManus et al. find that NLRP3
is located at mitochondria where it regulates microglial metabolism. Depletion or
chronic pharmacological inhibiti...
www.cell.com
February 3, 2025 at 5:51 PM
NLRP3-mediated glutaminolysis controls microglial phagocytosis to promote Alzheimer’s disease progression
www.cell.com/immunity/ful...
@cp-immunity.bsky.social @roisinmcmanus.bsky.social
www.cell.com/immunity/ful...
@cp-immunity.bsky.social @roisinmcmanus.bsky.social
Are there spatial restrictions in NLRP3 #inflammasome activation? I am super excited to share our new study spearheaded by amazing @elvirab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly - Nature Communications
NLRP3 inflammasome activation is critical for the induction of protective immunity, but molecular insights are still lacking. Here, the authors express NLRP3 variants targeted to different cellular lo...
www.nature.com
May 27, 2025 at 8:40 AM
Are there spatial restrictions in NLRP3 #inflammasome activation? I am super excited to share our new study spearheaded by amazing @elvirab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
A large dataset we hope is helpful for patients who may have CAPS, an #autoinflammatory condition associated with genetic variants in NLRP3. Lots of "variants of uncertain significance" now have a specific readout from our in vitro assay which can help with clinical diagnosis.
rdcu.be/d9iRG
rdcu.be/d9iRG
Mechanisms of NLRP3 activation and inhibition elucidated by functional analysis of disease-associated variants
Nature Immunology - Gain-of-function variants in the gene encoding NLRP3 lead to constitutive inflammasome activation and excessive IL-1β production. In this resource, authors perform...
rdcu.be
February 11, 2025 at 9:57 AM
A large dataset we hope is helpful for patients who may have CAPS, an #autoinflammatory condition associated with genetic variants in NLRP3. Lots of "variants of uncertain significance" now have a specific readout from our in vitro assay which can help with clinical diagnosis.
rdcu.be/d9iRG
rdcu.be/d9iRG
Researchers screening "nutrients & metabolites" found that l-Arginine inhibits NLRP3 inflammasome assembly in vitro. It binds to the Asp31 site of the NLRP3 protein, blocking its interaction with ASC. L-Arginine levels significantly⬇️in #Parkinsons serum
www.nature.com/articles/s42...
www.nature.com/articles/s42...
October 7, 2026 at 9:41 PM
Researchers screening "nutrients & metabolites" found that l-Arginine inhibits NLRP3 inflammasome assembly in vitro. It binds to the Asp31 site of the NLRP3 protein, blocking its interaction with ASC. L-Arginine levels significantly⬇️in #Parkinsons serum
www.nature.com/articles/s42...
www.nature.com/articles/s42...
NLRP3 inflammasomes can assemble on most organelles. A beautiful study by Hafner et al demonstrate the flexibility of organelles that can support inflammasome activation. Kudos to the authors on an intriguing study.
www.nature.com/articles/s41...
@ivahb.bsky.social @elvirab.bsky.social
www.nature.com/articles/s41...
@ivahb.bsky.social @elvirab.bsky.social
Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly - Nature Communications
NLRP3 inflammasome activation is critical for the induction of protective immunity, but molecular insights are still lacking. Here, the authors express NLRP3 variants targeted to different cellular lo...
www.nature.com
May 27, 2025 at 1:31 PM
NLRP3 inflammasomes can assemble on most organelles. A beautiful study by Hafner et al demonstrate the flexibility of organelles that can support inflammasome activation. Kudos to the authors on an intriguing study.
www.nature.com/articles/s41...
@ivahb.bsky.social @elvirab.bsky.social
www.nature.com/articles/s41...
@ivahb.bsky.social @elvirab.bsky.social
I am excited to share with you an amazing video on the NLRP3 inflammasome pathway generated by talented Ann Liu from the Janet Iwasa lab @jiwasa.bsky.social @ann-hl.bsky.social. It shows the real structures of the pathway from #NLRP3, #ASC, #caspase-1, #GSDMD, #IL-1b, to #NINJ1. Enjoy!
March 6, 2026 at 7:48 PM
I am excited to share with you an amazing video on the NLRP3 inflammasome pathway generated by talented Ann Liu from the Janet Iwasa lab @jiwasa.bsky.social @ann-hl.bsky.social. It shows the real structures of the pathway from #NLRP3, #ASC, #caspase-1, #GSDMD, #IL-1b, to #NINJ1. Enjoy!
Mechanisms of NLRP3 activation and inhibition elucidated by functional analysis of disease-associated variants
www.nature.com/articles/s41...
@natimmunol.bsky.social @sethlucianmasters.bsky.social
www.nature.com/articles/s41...
@natimmunol.bsky.social @sethlucianmasters.bsky.social
February 10, 2025 at 3:32 PM
Mechanisms of NLRP3 activation and inhibition elucidated by functional analysis of disease-associated variants
www.nature.com/articles/s41...
@natimmunol.bsky.social @sethlucianmasters.bsky.social
www.nature.com/articles/s41...
@natimmunol.bsky.social @sethlucianmasters.bsky.social
More nice research from Annie Curtis’ lab on the circadian rhythm and the
#immunometabolism of macrophages.
#immunosky #mitochondria #inflammasome
Time‐of‐day control of mitochondria regulates NLRP3 inflammasome activation in macrophages faseb.onlinelibrary.wiley.com/doi/10.1096/...
#immunometabolism of macrophages.
#immunosky #mitochondria #inflammasome
Time‐of‐day control of mitochondria regulates NLRP3 inflammasome activation in macrophages faseb.onlinelibrary.wiley.com/doi/10.1096/...
Time‐of‐day control of mitochondria regulates NLRP3 inflammasome activation in macrophages
The circadian clock, orchestrated by BMAL1, regulates the expression of Nlrp3 and Il1b in response to lipopolysaccharide (LPS), causing time-of-day production of pro-IL-1β. Mitochondria exhibit time-....
faseb.onlinelibrary.wiley.com
January 15, 2025 at 12:31 AM
More nice research from Annie Curtis’ lab on the circadian rhythm and the
#immunometabolism of macrophages.
#immunosky #mitochondria #inflammasome
Time‐of‐day control of mitochondria regulates NLRP3 inflammasome activation in macrophages faseb.onlinelibrary.wiley.com/doi/10.1096/...
#immunometabolism of macrophages.
#immunosky #mitochondria #inflammasome
Time‐of‐day control of mitochondria regulates NLRP3 inflammasome activation in macrophages faseb.onlinelibrary.wiley.com/doi/10.1096/...
Wirts aktivieren. Aktuelle Forschungsergebnisse belegen, dass diese Proteine auf zwei Hauptmechanismen wirken: [1] die Aktivierung glialer angeborener Immunrezeptoren, insbesondere TLR4 und TLR2, und die nachfolgende Bildung des NLRP3-Inflammasoms, wodurch eine gemeinsame,
June 7, 2026 at 8:33 PM
Wirts aktivieren. Aktuelle Forschungsergebnisse belegen, dass diese Proteine auf zwei Hauptmechanismen wirken: [1] die Aktivierung glialer angeborener Immunrezeptoren, insbesondere TLR4 und TLR2, und die nachfolgende Bildung des NLRP3-Inflammasoms, wodurch eine gemeinsame,
Why is obesity often associated with dysregulated, low-grade chronic inflammation?
In our new paper in @science.org we show that obesity rewires nucleotide metabolism in immune cells, making inflammatory responses much easier to trigger. www.science.org/doi/10.1126/...
In our new paper in @science.org we show that obesity rewires nucleotide metabolism in immune cells, making inflammatory responses much easier to trigger. www.science.org/doi/10.1126/...
Nucleotide metabolic rewiring enables NLRP3 inflammasome hyperactivation in obesity
Obesity is a major disease risk factor due to obesity-associated hyperinflammation. We found that obesity induced Nod-like receptor pyrin domain–containing 3 (NLRP3) inflammasome hyperactivation and e...
www.science.org
January 16, 2026 at 6:14 PM
Why is obesity often associated with dysregulated, low-grade chronic inflammation?
In our new paper in @science.org we show that obesity rewires nucleotide metabolism in immune cells, making inflammatory responses much easier to trigger. www.science.org/doi/10.1126/...
In our new paper in @science.org we show that obesity rewires nucleotide metabolism in immune cells, making inflammatory responses much easier to trigger. www.science.org/doi/10.1126/...
Acute suppression of mitochondrial ATP production prevents apoptosis and provides an essential signal for NLRP3 inflammasome activation
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
Acute suppression of mitochondrial ATP production prevents apoptosis and provides an essential signal for NLRP3 inflammasome activation
Mitochondria control metabolism, cell death, and innate immunity, yet their reciprocal
mechanistic connections are elusive, as is the conserved physiological function of
the NLRP3 inflammasome. Saller...
www.cell.com
November 20, 2024 at 10:48 PM
Acute suppression of mitochondrial ATP production prevents apoptosis and provides an essential signal for NLRP3 inflammasome activation
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
Thrilled to share our new preprint showing that dietary amino acids license the NLRP3 inflammasome via mTOR-dependent mRNA translation! Congrats to co-1st authors @mikelhaggadone.bsky.social, Brian Goldspiel, co-senior author @metabailism.bsky.social & collaborators! www.biorxiv.org/content/10.6...
January 21, 2026 at 5:01 PM
Thrilled to share our new preprint showing that dietary amino acids license the NLRP3 inflammasome via mTOR-dependent mRNA translation! Congrats to co-1st authors @mikelhaggadone.bsky.social, Brian Goldspiel, co-senior author @metabailism.bsky.social & collaborators! www.biorxiv.org/content/10.6...
🎉 Huge congratulations to @roisinmcmanus.bsky.social and Michael Heneka on this exciting study uncovering a novel role for NLRP3 in regulating microglial metabolism and phagocytosis to promote Alzheimer’s disease progression www.cell.com/immunity/abs...
NLRP3-mediated glutaminolysis controls microglial phagocytosis to promote Alzheimer’s disease progression
How NLRP3 impacts Alzheimer’s disease (AD) is unclear. McManus et al. find that NLRP3
is located at mitochondria where it regulates microglial metabolism. Depletion or
chronic pharmacological inhibiti...
www.cell.com
February 6, 2025 at 8:35 PM
🎉 Huge congratulations to @roisinmcmanus.bsky.social and Michael Heneka on this exciting study uncovering a novel role for NLRP3 in regulating microglial metabolism and phagocytosis to promote Alzheimer’s disease progression www.cell.com/immunity/abs...
Excited to share our paper on the NLRP3 inflammasome condensate at the Microtubule Organizing Center (MTOC) by "precise!" cryo-electron tomography (cryo-ET), in collaboration with Jue (phyllis) Wang in the labs of Grant Jensen (BYU) and Pete Dahlberg (Stanford)! www.science.org/doi/10.1126/...
April 23, 2026 at 6:43 PM
Excited to share our paper on the NLRP3 inflammasome condensate at the Microtubule Organizing Center (MTOC) by "precise!" cryo-electron tomography (cryo-ET), in collaboration with Jue (phyllis) Wang in the labs of Grant Jensen (BYU) and Pete Dahlberg (Stanford)! www.science.org/doi/10.1126/...
#WeekendRead! #ImmunometabolismPower! #InflammasomePower! Liu, Yin &co show @cp-cellchembiol that lactate downstream of aerobic glycolysis is used to directly lactylates NLRP3 increasing ASC recruitment and inflammasome activation, which exacerbates lethality in sepsis! www.cell.com/cell-chemica...
Aerobic glycolysis promotes NLRP3 inflammasome activation via NLRP3 lactylation
Liu et al. reveal that lactate, an aerobic glycolysis product, promotes NLRP3 inflammasome
activation and pyroptosis. NLRP3 is identified as a target of lactylation, and K24
and K565 have potential to...
www.cell.com
February 7, 2026 at 3:59 PM
#WeekendRead! #ImmunometabolismPower! #InflammasomePower! Liu, Yin &co show @cp-cellchembiol that lactate downstream of aerobic glycolysis is used to directly lactylates NLRP3 increasing ASC recruitment and inflammasome activation, which exacerbates lethality in sepsis! www.cell.com/cell-chemica...
@biorxivpreprint.bsky.social CRY-NLRP3 complexes are a circadian checkpoint that modulates inflammasome activity and drug response, revealing time of day as a critical dimension of NLRP3-driven inflammation
www.biorxiv.org/content/10.6... @inserm.fr
www.biorxiv.org/content/10.6... @inserm.fr
March 2, 2026 at 3:34 AM
@biorxivpreprint.bsky.social CRY-NLRP3 complexes are a circadian checkpoint that modulates inflammasome activity and drug response, revealing time of day as a critical dimension of NLRP3-driven inflammation
www.biorxiv.org/content/10.6... @inserm.fr
www.biorxiv.org/content/10.6... @inserm.fr
RRIDs were included in this in Journal of Musculoskeletal and Neuronal Interactions paper. We value the author's support of reproducibility. #STMpublishing #reproducibility #RRID
Astragalus Polysaccharides Attenuate LPS-Induced Degenerative Responses in Primary Rat Chondrocytes linked to the NLRP3/CASPASE-1 Axis
Read the full paper: Astragalus Polysaccharides Attenuate LPS-Induced Degenerative Responses in Primary Rat Chondrocytes linked to the NLRP3/CASPASE-1 Axis
doi.org
June 4, 2026 at 7:01 AM
RRIDs were included in this in Journal of Musculoskeletal and Neuronal Interactions paper. We value the author's support of reproducibility. #STMpublishing #reproducibility #RRID
Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly
www.nature.com/articles/s41...
@natcomms.nature.com
www.nature.com/articles/s41...
@natcomms.nature.com
Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly - Nature Communications
NLRP3 inflammasome activation is critical for the induction of protective immunity, but molecular insights are still lacking. Here, the authors express NLRP3 variants targeted to different cellular lo...
www.nature.com
May 28, 2025 at 2:22 AM
Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly
www.nature.com/articles/s41...
@natcomms.nature.com
www.nature.com/articles/s41...
@natcomms.nature.com
What orchestrates the dance of innate immunity? Lauren Spector and I reflect on how the NLRP3 inflammasome dynamically interacts with organelles to coordinate immunity—thanks to the remarkable work of researchers in the field! journals.lww.com/immunometabo...
Revealing the dance of NLRP3: spatiotemporal patterns in... : Immunometabolism
y of autoimmune and inflammatory diseases, including cryopyrin-associated periodic fever syndromes, diabetes, atherosclerosis, Alzheimer’s disease, inflammatory bowel disease, and cancer. Consequently...
journals.lww.com
January 29, 2025 at 1:30 AM
What orchestrates the dance of innate immunity? Lauren Spector and I reflect on how the NLRP3 inflammasome dynamically interacts with organelles to coordinate immunity—thanks to the remarkable work of researchers in the field! journals.lww.com/immunometabo...