Thrilled to start 2025 with our latest findings! Yersinia invades macrophage cytosol to activate caspase-11 when caspase-8 or YopJ are inactive. www.cell.com/cell-reports...
Plasticity of cell death pathways ensures GSDMD activation during Yersinia pseudotuberculosis infection
Injection of YopJ by Yersinia pseudotuberculosis triggers RIPK1-dependent caspase-8
and GSDMD activation in murine macrophages; however, how macrophages defend against
Yersinia when caspase-8 or YopJ ...
www.cell.com
January 16, 2025 at 11:09 PM
Thrilled to start 2025 with our latest findings! Yersinia invades macrophage cytosol to activate caspase-11 when caspase-8 or YopJ are inactive. www.cell.com/cell-reports...
Delivery of caspase inhibitors through GSDMD pores to inhibit pyroptosis https://www.biorxiv.org/content/10.1101/2025.02.11.637513v1
February 12, 2025 at 2:30 AM
Delivery of caspase inhibitors through GSDMD pores to inhibit pyroptosis https://www.biorxiv.org/content/10.1101/2025.02.11.637513v1
#Fumarate update
Gang of 3: How the Krebs cycle-linked metabolites itaconate, succinate, and fumarate regulate macrophages & inflammation
Dual role in Inflammation
Anti-
KEAP1-NRF2
GSDMD
NLRP3
IL-10
Pro-
mtDNA mtRNA➡️IFNβ
#CellMetab 2025
www.sciencedirect.com/science/arti...
Gang of 3: How the Krebs cycle-linked metabolites itaconate, succinate, and fumarate regulate macrophages & inflammation
Dual role in Inflammation
Anti-
KEAP1-NRF2
GSDMD
NLRP3
IL-10
Pro-
mtDNA mtRNA➡️IFNβ
#CellMetab 2025
www.sciencedirect.com/science/arti...
June 21, 2025 at 7:47 PM
#Fumarate update
Gang of 3: How the Krebs cycle-linked metabolites itaconate, succinate, and fumarate regulate macrophages & inflammation
Dual role in Inflammation
Anti-
KEAP1-NRF2
GSDMD
NLRP3
IL-10
Pro-
mtDNA mtRNA➡️IFNβ
#CellMetab 2025
www.sciencedirect.com/science/arti...
Gang of 3: How the Krebs cycle-linked metabolites itaconate, succinate, and fumarate regulate macrophages & inflammation
Dual role in Inflammation
Anti-
KEAP1-NRF2
GSDMD
NLRP3
IL-10
Pro-
mtDNA mtRNA➡️IFNβ
#CellMetab 2025
www.sciencedirect.com/science/arti...
ⓕⓛⓨⓘⓝⓖ ⓔⓜⓑⓔⓡⓢ
🔥 🔥 🔥 🔥 🔥 🔥 🔥 🔥
ⓘⓝ ⓐ ⓦⓘⓛⓓⓕⓘⓡⓔ
new concept in the propagation of inflammatory cell death in severe inflammation and sepsis
extracellular vesicles released from dying cells contain gasdermin pores that can subsequently attack and initiate rapid apoptosis in bystander cells
🔥 PyroEVs 🔥
🔥 🔥 🔥 🔥 🔥 🔥 🔥 🔥
ⓘⓝ ⓐ ⓦⓘⓛⓓⓕⓘⓡⓔ
new concept in the propagation of inflammatory cell death in severe inflammation and sepsis
extracellular vesicles released from dying cells contain gasdermin pores that can subsequently attack and initiate rapid apoptosis in bystander cells
🔥 PyroEVs 🔥
Transplantation of gasdermin pores by extracellular vesicles propagates pyroptosis to bystander cells
Expanding the consequences of pyroptosis, this study reveals that GSDMD pore-laden
pyroptotic EVs transplant GSDMD pores on neighboring cell surfaces and propagate cell
death to bystander cells.
www.cell.com
January 26, 2025 at 12:57 PM
ⓕⓛⓨⓘⓝⓖ ⓔⓜⓑⓔⓡⓢ
🔥 🔥 🔥 🔥 🔥 🔥 🔥 🔥
ⓘⓝ ⓐ ⓦⓘⓛⓓⓕⓘⓡⓔ
new concept in the propagation of inflammatory cell death in severe inflammation and sepsis
extracellular vesicles released from dying cells contain gasdermin pores that can subsequently attack and initiate rapid apoptosis in bystander cells
🔥 PyroEVs 🔥
🔥 🔥 🔥 🔥 🔥 🔥 🔥 🔥
ⓘⓝ ⓐ ⓦⓘⓛⓓⓕⓘⓡⓔ
new concept in the propagation of inflammatory cell death in severe inflammation and sepsis
extracellular vesicles released from dying cells contain gasdermin pores that can subsequently attack and initiate rapid apoptosis in bystander cells
🔥 PyroEVs 🔥
I am excited to share with you an amazing video on the NLRP3 inflammasome pathway generated by talented Ann Liu from the Janet Iwasa lab @jiwasa.bsky.social @ann-hl.bsky.social. It shows the real structures of the pathway from #NLRP3, #ASC, #caspase-1, #GSDMD, #IL-1b, to #NINJ1. Enjoy!
March 6, 2026 at 7:48 PM
I am excited to share with you an amazing video on the NLRP3 inflammasome pathway generated by talented Ann Liu from the Janet Iwasa lab @jiwasa.bsky.social @ann-hl.bsky.social. It shows the real structures of the pathway from #NLRP3, #ASC, #caspase-1, #GSDMD, #IL-1b, to #NINJ1. Enjoy!
Very interesting pre-print from the group of Vishva Dixit in California! The @genentech.bsky.social team found the small-molecule inhibitors of caspase activity can be delivered using GSDMD pores as an entry point!
#Inflammasome #Immunology
www.biorxiv.org/content/10.1...
#Inflammasome #Immunology
www.biorxiv.org/content/10.1...
Delivery of caspase inhibitors through GSDMD pores to inhibit pyroptosis
Caspase-1, -4, -5, and -11 activate Gasdermin D (GSDMD) to form pores in the plasma membrane. In addition to releasing interleukin (IL)-1β and IL-18, GSDMD pores cause a lytic, proinflammatory form of...
www.biorxiv.org
February 17, 2025 at 8:17 AM
Very interesting pre-print from the group of Vishva Dixit in California! The @genentech.bsky.social team found the small-molecule inhibitors of caspase activity can be delivered using GSDMD pores as an entry point!
#Inflammasome #Immunology
www.biorxiv.org/content/10.1...
#Inflammasome #Immunology
www.biorxiv.org/content/10.1...
Online now! #chembiol
Visualization of calpain-1 activation during cell death and its role in GSDMD cleavage using chemical probes
Horbach et al. developed chemical tools to study calpain-1 activation during pyroptosis. Using mass cytometry and IQF substrates, the authors show that calpain-1 correlates with GSDMD and caspase-1 in immune cells. They also demonstrate that calpain-1 cleaves GSDMD at a putative QRTF/Q, potentially supporting pyroptosis process.
dlvr.it
March 28, 2025 at 3:30 PM
Online now! #chembiol
New findings show that allergens trigger PAR1-dependent ferritinophagy, raising intracellular iron levels, crucial for noncanonical GSDMD activation in allergic airway diseases. PMID:42361797, Cell 2026, @Cell https://www.cell.com/cell/fulltext/S0092-8674(26)00653-7 #Medsky #Pharmsky #RNA #ASHG 🧪
https://www.cell.com/cell/fulltext/S0092-8674(26)00653-7
No description available
www.cell.com
September 26, 2026 at 7:00 PM
🔬🦠 Unveiling the role of GSDMD in Streptococcus equi subsp. zooepidemicus infection! This study reveals the critical function of GSDMD in neutrophil extracellular traps (NETs) formation. 🌟🔍
Full article: link.springer.com/article/10.1... 📚🔗
#Neutrophils #GSDMD #ImmuneResponse
Full article: link.springer.com/article/10.1... 📚🔗
#Neutrophils #GSDMD #ImmuneResponse
March 11, 2026 at 7:01 AM
🔬🦠 Unveiling the role of GSDMD in Streptococcus equi subsp. zooepidemicus infection! This study reveals the critical function of GSDMD in neutrophil extracellular traps (NETs) formation. 🌟🔍
Full article: link.springer.com/article/10.1... 📚🔗
#Neutrophils #GSDMD #ImmuneResponse
Full article: link.springer.com/article/10.1... 📚🔗
#Neutrophils #GSDMD #ImmuneResponse
#NoTimeToDie! @vijayrathinam.bsky.social Weight &co show @cp-cell.bsky.social that pyroptotic cells release extracellular vesicles which transplant gasdermin D #GSDMD pores to neighboring cells propagating cell death! t.co/XgU6jt15aF Free link to the paper 👉 authors.elsevier.com/a/1kMQgL7PXq...
https://www.cell.com/cell/abstract/S0092-8674(24)01334-5
t.co
December 31, 2024 at 6:07 PM
#NoTimeToDie! @vijayrathinam.bsky.social Weight &co show @cp-cell.bsky.social that pyroptotic cells release extracellular vesicles which transplant gasdermin D #GSDMD pores to neighboring cells propagating cell death! t.co/XgU6jt15aF Free link to the paper 👉 authors.elsevier.com/a/1kMQgL7PXq...
🧬 Our new preprint showing gasdermin E is dispensable for inflammatory responses and pathogenesis in H1N1 influenza. Unlike our prior work on GSDMD. 🫁🦠🐁 www.biorxiv.org/content/10.1...
July 30, 2025 at 2:00 PM
🧬 Our new preprint showing gasdermin E is dispensable for inflammatory responses and pathogenesis in H1N1 influenza. Unlike our prior work on GSDMD. 🫁🦠🐁 www.biorxiv.org/content/10.1...
SK56, an artificial intelligence-screened peptide, effectively obstructs GSDMD-NT pores and inhibits pyroptosis and cytokine release in macrophages and human peripheral blood leukocyte-induced pyroptosis @natimmunol.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
September 15, 2025 at 4:26 PM
SK56, an artificial intelligence-screened peptide, effectively obstructs GSDMD-NT pores and inhibits pyroptosis and cytokine release in macrophages and human peripheral blood leukocyte-induced pyroptosis @natimmunol.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Filopodial projections crowed #pyroptotic & #necroptotic corpses, but not #apoptotic corpses - even when apoptosis was triggered by #inflammasome signalling in GSDMD-null cells. So filopodia = a flag that a cell lysed 'under suspicious circumstances' ⛳️ (7/13)
Meme: @arnaudjlegrand.bsky.social
Meme: @arnaudjlegrand.bsky.social
December 9, 2024 at 1:39 AM
Filopodial projections crowed #pyroptotic & #necroptotic corpses, but not #apoptotic corpses - even when apoptosis was triggered by #inflammasome signalling in GSDMD-null cells. So filopodia = a flag that a cell lysed 'under suspicious circumstances' ⛳️ (7/13)
Meme: @arnaudjlegrand.bsky.social
Meme: @arnaudjlegrand.bsky.social
Incredibly, minutes before pyroptotic #CellExplosion, cells assemble Myo10-capped #filopodia, which crown the resultant cell corpse. #inflammasome #GSDMD signalling was required for both filopodial assembly & cell death. Might Ca2+ influx via GSDMD pores be involved? 🤔(4/13)
December 9, 2024 at 1:39 AM
Incredibly, minutes before pyroptotic #CellExplosion, cells assemble Myo10-capped #filopodia, which crown the resultant cell corpse. #inflammasome #GSDMD signalling was required for both filopodial assembly & cell death. Might Ca2+ influx via GSDMD pores be involved? 🤔(4/13)
RING1 dictates GSDMD-mediated inflammatory response and host susceptibility to pathogen infection https://www.biorxiv.org/content/10.1101/2024.11.05.622030v1
RING1 dictates GSDMD-mediated inflammatory response and host susceptibility to pathogen infection https://www.biorxiv.org/content/10.1101/2024.11.05.622030v1
RING1 is an E3 ligase component of the polycomb repressive complex 1 (PRC1) with known roles in chro
www.biorxiv.org
November 7, 2024 at 7:31 AM
RING1 dictates GSDMD-mediated inflammatory response and host susceptibility to pathogen infection https://www.biorxiv.org/content/10.1101/2024.11.05.622030v1
#AIC2024 come visit my talk “GSDMD promotes influenza virus induced mortality through neutrophil amplification of inflammation” in workshop 6 (abstract 59) 🐁🦠
November 23, 2024 at 5:36 PM
#AIC2024 come visit my talk “GSDMD promotes influenza virus induced mortality through neutrophil amplification of inflammation” in workshop 6 (abstract 59) 🐁🦠
Disulfiram Protects Against Diet-Induced Obesity by Reprogramming Systemic Lipid Partitioning Independent of GSDMD https://www.biorxiv.org/content/10.64898/2026.02.06.704424v1
February 10, 2026 at 2:00 AM
Disulfiram Protects Against Diet-Induced Obesity by Reprogramming Systemic Lipid Partitioning Independent of GSDMD https://www.biorxiv.org/content/10.64898/2026.02.06.704424v1
Played around a bit with Blender over the weekend and made a movie of a computational assay I had set up a while ago.
Gasdermin-D binds to PIP lipids and I wanted to know what impact it has to just populate the most prominent binding site. PIP3, even more than PIP2, stabilizes GSDMD oligomers!
Gasdermin-D binds to PIP lipids and I wanted to know what impact it has to just populate the most prominent binding site. PIP3, even more than PIP2, stabilizes GSDMD oligomers!
September 14, 2026 at 10:34 AM
Played around a bit with Blender over the weekend and made a movie of a computational assay I had set up a while ago.
Gasdermin-D binds to PIP lipids and I wanted to know what impact it has to just populate the most prominent binding site. PIP3, even more than PIP2, stabilizes GSDMD oligomers!
Gasdermin-D binds to PIP lipids and I wanted to know what impact it has to just populate the most prominent binding site. PIP3, even more than PIP2, stabilizes GSDMD oligomers!
Not only ASC specks and inflammatory cytokines, but gasdermin pores are found to be shared during pyroptosis! #Inflammasome #Immunology
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Transplantation of gasdermin pores by extracellular vesicles propagates pyroptosis to bystander cells
Expanding the consequences of pyroptosis, this study reveals that GSDMD pore-laden pyroptotic EVs transplant GSDMD pores on neighboring cell surfaces and propagate cell death to bystander cells.
www.cell.com
January 2, 2025 at 8:21 AM
Not only ASC specks and inflammatory cytokines, but gasdermin pores are found to be shared during pyroptosis! #Inflammasome #Immunology
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Congratulations to Jingyi Shen on the completion of her PhD! Shen, a researcher in the Obenauf lab, presented her dissertation titled “Exploring the immunogenicity of cell death through the induction of GSDMD-mediated pyroptosis in cancer cells” during her PhD defense.
March 23, 2026 at 11:00 AM
Congratulations to Jingyi Shen on the completion of her PhD! Shen, a researcher in the Obenauf lab, presented her dissertation titled “Exploring the immunogenicity of cell death through the induction of GSDMD-mediated pyroptosis in cancer cells” during her PhD defense.
Original study finding GSDMD regulation by mito ROS, downstream of cleavage.
pubmed.ncbi.nlm.nih.gov/34289345/
pubmed.ncbi.nlm.nih.gov/34289345/
Control of gasdermin D oligomerization and pyroptosis by the Ragulator-Rag-mTORC1 pathway - PubMed
The process of pyroptosis is mediated by inflammasomes and a downstream effector known as gasdermin D (GSDMD). Upon cleavage by inflammasome-associated caspases, the N-terminal domain of GSDMD forms m...
pubmed.ncbi.nlm.nih.gov
February 19, 2025 at 4:37 PM
Original study finding GSDMD regulation by mito ROS, downstream of cleavage.
pubmed.ncbi.nlm.nih.gov/34289345/
pubmed.ncbi.nlm.nih.gov/34289345/
#Sepsis
DLL4+ Neutrophil induces PANoptosis of Septic 🫁 Notch1+ Endothelial Cell in CLP🐭
DLL4+ Neutrophil ⏫by extracellular cold-inducible RNA-binding protein
PANoptosis is a ZBP1-induced integrated death mode (cleaved GSDMD+, cleaved caspase 3+, pMLKL+)
#JCI 2025
www.jci.org/articles/vie...
DLL4+ Neutrophil induces PANoptosis of Septic 🫁 Notch1+ Endothelial Cell in CLP🐭
DLL4+ Neutrophil ⏫by extracellular cold-inducible RNA-binding protein
PANoptosis is a ZBP1-induced integrated death mode (cleaved GSDMD+, cleaved caspase 3+, pMLKL+)
#JCI 2025
www.jci.org/articles/vie...
March 13, 2026 at 12:15 PM
#Sepsis
DLL4+ Neutrophil induces PANoptosis of Septic 🫁 Notch1+ Endothelial Cell in CLP🐭
DLL4+ Neutrophil ⏫by extracellular cold-inducible RNA-binding protein
PANoptosis is a ZBP1-induced integrated death mode (cleaved GSDMD+, cleaved caspase 3+, pMLKL+)
#JCI 2025
www.jci.org/articles/vie...
DLL4+ Neutrophil induces PANoptosis of Septic 🫁 Notch1+ Endothelial Cell in CLP🐭
DLL4+ Neutrophil ⏫by extracellular cold-inducible RNA-binding protein
PANoptosis is a ZBP1-induced integrated death mode (cleaved GSDMD+, cleaved caspase 3+, pMLKL+)
#JCI 2025
www.jci.org/articles/vie...
Delivery of caspase inhibitors through GSDMD pores to inhibit pyroptosis https://www.biorxiv.org/content/10.1101/2025.02.11.637513v1
February 12, 2025 at 2:30 AM
Delivery of caspase inhibitors through GSDMD pores to inhibit pyroptosis https://www.biorxiv.org/content/10.1101/2025.02.11.637513v1