YouTube video by Black Liberation Media

YouTube video by Leo Kottke - Topic

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) enables the direct visualization of metabolites from tissue sections with high spatial resolution. However, its application to untargeted spatial metabolomics is hindered by poor ionizing compounds and challenges in accurate metabolite annotation. On-tissue chemical derivatization (OTCD) is commonly employed to enhance the ionization of metabolites bearing specific functional groups, and platforms such as METASPACE facilitate high-throughput annotation of derivatized features. Nevertheless, distinguishing structural isomers for a large number of metabolites remains a major challenge, often resulting in incorrect annotations. To address this limitation, we developed an improved annotation workflow for OTCD-MALDI-MSI by integrating two filtering strategies. Functional group filtering leverages SMARTS-based substructure matching to retain only those metabolites that react with the applied OTCD reagent. In parallel, gas-phase hydrogen–deuterium exchange (HDX) in the MALDI source is used to determine the number of labile hydrogens for each feature, enabling the exclusion of annotations that are inconsistent with HDX behavior. We applied this workflow to MALDI-MSI of maize root sections using Girard’s reagents T and P, along with the plant-specific COCONUT metabolite database. The combined filtering strategy reduced incorrect annotations by ∼67%, from ∼7.3 annotations per unique feature without filtering to ∼2.4 with filtering, substantially improving annotation accuracy and confidence. By coupling OTCD signal enhancement with structurally informed filtering, this workflow advances the utility of MALDI-MSI for untargeted spatial metabolomics, enabling more reliable and scalable metabolite profiling in complex biological tissues.

YouTube video by Clave geopolítica

YouTube video by James Horner - Topic

Ultragenyx's gene therapy DTX301 shows positive Phase 3 results for rare metabolic disease OTCD, reducing plasma ammonia levels by 18% vs placebo at 36 weeks.

### Research Paper: Closed-Loop Adenine Base Editing (CLABE) for Efficient and Precise Correction of Ornithine Transcarbamylase Deficiency (OTCD) in vivo **Abstract:** Ornithine transcarbamylase deficiency (OTCD), a severe X-linked urea cycle disorder, currently lacks a curative therapy. This research proposes a novel closed-loop adenine base editing (CLABE) system utilizing CRISPR-associated adenine base editors (ABEs) delivered via adeno-associated virus (AAV) for in vivo correction of OTCD in murine models.

YouTube video by Mrs. Fluffy

YouTube video by Linda Ronstadt - Topic

Inaresto ng NBI-OTCD ang 5 Vietnamese para sa ilegal na pagbebenta umano ng dog meat sa Makati City. | via @ZyannAmbrosio https://t.co/RJFb1UI7pk

YouTube video by Middlesex-London Health Unit

This is probably the last time I can make this joke, I doubt I'll ever get 69 Royal Fumble or Kraken Slam wins.

Dr. Jim Wilson, a gene therapy innovator, shares insights on iECURE's promising results for ECUR-506 in treating Ornithine Transcarbamylase Deficiency, marking a key milestone in biotechnology.

National Bureau of Investigation agents are seen in a video of the shooting with the Senate Office of the Sergeant-at-Arms on Wednesday, May 13, 2026. — Screengrab from NBI-OTCD MANILA,

YouTube video by Peter Lewis

YouTube video by Release - Topic