This Father's Day, don't believe everything you read on the internet. But read this. #MenCare #CareCantWait https://globalstrategygroup.com/wp-content/uploads/2025/06/PLFA-Men-Survey-Memo-F06.02.25.pdf
June 11, 2025 at 7:45 PM
This Father's Day, don't believe everything you read on the internet. But read this. #MenCare #CareCantWait https://globalstrategygroup.com/wp-content/uploads/2025/06/PLFA-Men-Survey-Memo-F06.02.25.pdf
I feared becoming a prof would mean not doing some things I rly value. Yet these last weeks, I did loads of lab work (👋 again PLFA) and bioinformatics (new ONT long read data 🫶), and all between teaching, paper writing, grant chats, and meetings (so many). It’s a great job and I’m lucky to be in 🇦🇹!
June 12, 2025 at 6:38 PM
I feared becoming a prof would mean not doing some things I rly value. Yet these last weeks, I did loads of lab work (👋 again PLFA) and bioinformatics (new ONT long read data 🫶), and all between teaching, paper writing, grant chats, and meetings (so many). It’s a great job and I’m lucky to be in 🇦🇹!
Check out our new preprint
‚Soil fungi remain active and invest in storage compounds during drought independent of future climate conditions‘
by @loutsi.bsky.social, Alberto Canarini, and many more.
➡️2H-vapor-PLFA-SIP
biorxiv.org/cgi/content/...
‚Soil fungi remain active and invest in storage compounds during drought independent of future climate conditions‘
by @loutsi.bsky.social, Alberto Canarini, and many more.
➡️2H-vapor-PLFA-SIP
biorxiv.org/cgi/content/...
October 24, 2023 at 4:39 PM
Check out our new preprint
‚Soil fungi remain active and invest in storage compounds during drought independent of future climate conditions‘
by @loutsi.bsky.social, Alberto Canarini, and many more.
➡️2H-vapor-PLFA-SIP
biorxiv.org/cgi/content/...
‚Soil fungi remain active and invest in storage compounds during drought independent of future climate conditions‘
by @loutsi.bsky.social, Alberto Canarini, and many more.
➡️2H-vapor-PLFA-SIP
biorxiv.org/cgi/content/...
“Busy start to 2025! Freeze-drying soil samples from Wakelyns for PLFA analysis & DNA extraction, tackling plenty of admin, and squeezing in some fun at #FOG2025 Festival of Genomics in London. #SoilScience #Microbiology #Genomics #PLFA #DNAExtraction #Agroecology”
February 13, 2025 at 9:29 AM
“Busy start to 2025! Freeze-drying soil samples from Wakelyns for PLFA analysis & DNA extraction, tackling plenty of admin, and squeezing in some fun at #FOG2025 Festival of Genomics in London. #SoilScience #Microbiology #Genomics #PLFA #DNAExtraction #Agroecology”
2/2
For this study, we developed a new method, #2H-vapor-PLFA-SIP, which labels soil with 2H-labelled water vapor, allowing to study the activity/growth of microbial communities without altering the soil moisture. Thanks to all involved!
#TER @cemess.bsky.social, @univie.ac.at vie.ac.at
For this study, we developed a new method, #2H-vapor-PLFA-SIP, which labels soil with 2H-labelled water vapor, allowing to study the activity/growth of microbial communities without altering the soil moisture. Thanks to all involved!
#TER @cemess.bsky.social, @univie.ac.at vie.ac.at
Excited to share our new study in @naturecomms.bsky.social showing that soil fungi, unlike bacteria, remain fully active under severe drought conditions, and also invest in synthesis of storage compounds.
Led by the amazing @loutsi.bsky.social and Alberto Canarini.
Thread 1/2
rdcu.be/d1Tyg
Led by the amazing @loutsi.bsky.social and Alberto Canarini.
Thread 1/2
rdcu.be/d1Tyg
Soil fungi remain active and invest in storage compounds during drought independent of future climate conditions
Nature Communications - How climate change will impact microbial community growth is unclear. Here, the authors use a field experiment with varying global change factors, finding fungal growth more...
rdcu.be
November 29, 2024 at 6:40 PM
2/2
For this study, we developed a new method, #2H-vapor-PLFA-SIP, which labels soil with 2H-labelled water vapor, allowing to study the activity/growth of microbial communities without altering the soil moisture. Thanks to all involved!
#TER @cemess.bsky.social, @univie.ac.at vie.ac.at
For this study, we developed a new method, #2H-vapor-PLFA-SIP, which labels soil with 2H-labelled water vapor, allowing to study the activity/growth of microbial communities without altering the soil moisture. Thanks to all involved!
#TER @cemess.bsky.social, @univie.ac.at vie.ac.at
Bacterial necromass decomposition and #priming effects in #soils depend on #fertilization
NEW in @SoilBiolBiochem
doi.org/10.1016/j.so...
50-day Free: authors.elsevier.com/c/1lsrL_tPjA...
#SoilOrganicMatter #soil #primingeffect #CO2 #CH4 #microbialbiomass #PLFA #Fertilisers #soilbacteria #paddy
NEW in @SoilBiolBiochem
doi.org/10.1016/j.so...
50-day Free: authors.elsevier.com/c/1lsrL_tPjA...
#SoilOrganicMatter #soil #primingeffect #CO2 #CH4 #microbialbiomass #PLFA #Fertilisers #soilbacteria #paddy
October 2, 2025 at 7:36 AM
Bacterial necromass decomposition and #priming effects in #soils depend on #fertilization
NEW in @SoilBiolBiochem
doi.org/10.1016/j.so...
50-day Free: authors.elsevier.com/c/1lsrL_tPjA...
#SoilOrganicMatter #soil #primingeffect #CO2 #CH4 #microbialbiomass #PLFA #Fertilisers #soilbacteria #paddy
NEW in @SoilBiolBiochem
doi.org/10.1016/j.so...
50-day Free: authors.elsevier.com/c/1lsrL_tPjA...
#SoilOrganicMatter #soil #primingeffect #CO2 #CH4 #microbialbiomass #PLFA #Fertilisers #soilbacteria #paddy
Miikka B. Laine et al. demonstrate a refined phospholipid fatty acid (PLFA) analysis protocol that improves lipid separation and quantification in soil samples, revealing that freeze-dried storage at −80 °C preserves microbial community ratios better than −20 °C storage.
Soil Biology and Biochemistry
www.sciencedirect.com
September 26, 2026 at 6:22 AM
Miikka B. Laine et al. demonstrate a refined phospholipid fatty acid (PLFA) analysis protocol that improves lipid separation and quantification in soil samples, revealing that freeze-dried storage at −80 °C preserves microbial community ratios better than −20 °C storage.
Central PA people! Join us tomorrow (Tues) at the state capitol to make some noise for paid leave. We have a real shot of passing this historic legislation this session. PLUS: free shirts & lunch. RSVP-
forms.office.com/pages/respon... #momsky #pennsylvania #FamilyCareAct #PLFA #PaidLeave #PAMoms
forms.office.com/pages/respon... #momsky #pennsylvania #FamilyCareAct #PLFA #PaidLeave #PAMoms
May 5, 2025 at 4:49 PM
Central PA people! Join us tomorrow (Tues) at the state capitol to make some noise for paid leave. We have a real shot of passing this historic legislation this session. PLUS: free shirts & lunch. RSVP-
forms.office.com/pages/respon... #momsky #pennsylvania #FamilyCareAct #PLFA #PaidLeave #PAMoms
forms.office.com/pages/respon... #momsky #pennsylvania #FamilyCareAct #PLFA #PaidLeave #PAMoms
FCES 2025: Miikka B. Laine (@uniofjyvaskyla) talking about harmonising a PLFA method for studying microbial communities in agricultural soils.
May 22, 2025 at 8:33 AM
FCES 2025: Miikka B. Laine (@uniofjyvaskyla) talking about harmonising a PLFA method for studying microbial communities in agricultural soils.
How do you exist? I studied plfa and managed to show and complete most of the puzzles, but some eluded me.
I have never met a physics person who also loved dependent types.
I apologize if I am too enthusiastic but I am so glad you exist
I have never met a physics person who also loved dependent types.
I apologize if I am too enthusiastic but I am so glad you exist
September 20, 2026 at 3:18 AM
How do you exist? I studied plfa and managed to show and complete most of the puzzles, but some eluded me.
I have never met a physics person who also loved dependent types.
I apologize if I am too enthusiastic but I am so glad you exist
I have never met a physics person who also loved dependent types.
I apologize if I am too enthusiastic but I am so glad you exist
New publication: A global #database of #soil microbial phospholipid fatty #acids and #enzyme activities. #microbialcommunities
doi.org/10.1038/s415...
doi.org/10.1038/s415...
October 9, 2025 at 8:59 AM
New publication: A global #database of #soil microbial phospholipid fatty #acids and #enzyme activities. #microbialcommunities
doi.org/10.1038/s415...
doi.org/10.1038/s415...
Final version in @plantandsoil
Mycorrhizal growth and resource exchange define coexistence of plants
doi.org/10.1007/s111...
#carbonsequestration #soil #agriculture #Roots #microbialbiomass #PLFA #Fertilisers #nitrogen #rhizosphere #13Clabeling #isotopes
Mycorrhizal growth and resource exchange define coexistence of plants
doi.org/10.1007/s111...
#carbonsequestration #soil #agriculture #Roots #microbialbiomass #PLFA #Fertilisers #nitrogen #rhizosphere #13Clabeling #isotopes
May 30, 2025 at 12:40 PM
Final version in @plantandsoil
Mycorrhizal growth and resource exchange define coexistence of plants
doi.org/10.1007/s111...
#carbonsequestration #soil #agriculture #Roots #microbialbiomass #PLFA #Fertilisers #nitrogen #rhizosphere #13Clabeling #isotopes
Mycorrhizal growth and resource exchange define coexistence of plants
doi.org/10.1007/s111...
#carbonsequestration #soil #agriculture #Roots #microbialbiomass #PLFA #Fertilisers #nitrogen #rhizosphere #13Clabeling #isotopes
💥NEW in @SoilBiolBiochem
Aggregates mediate stability & temperature sensitivity of #soil #carbon in response to #biochar & #straw amendments
doi.org/10.1016/j.so...
50-day Free: authors.elsevier.com/c/1lkOK8g13f...
#SoilOrganicMatter #soilaggregates #enzymes #Q10 #PLFA #GlobalWarming
Aggregates mediate stability & temperature sensitivity of #soil #carbon in response to #biochar & #straw amendments
doi.org/10.1016/j.so...
50-day Free: authors.elsevier.com/c/1lkOK8g13f...
#SoilOrganicMatter #soilaggregates #enzymes #Q10 #PLFA #GlobalWarming
September 8, 2025 at 6:25 AM
💥NEW in @SoilBiolBiochem
Aggregates mediate stability & temperature sensitivity of #soil #carbon in response to #biochar & #straw amendments
doi.org/10.1016/j.so...
50-day Free: authors.elsevier.com/c/1lkOK8g13f...
#SoilOrganicMatter #soilaggregates #enzymes #Q10 #PLFA #GlobalWarming
Aggregates mediate stability & temperature sensitivity of #soil #carbon in response to #biochar & #straw amendments
doi.org/10.1016/j.so...
50-day Free: authors.elsevier.com/c/1lkOK8g13f...
#SoilOrganicMatter #soilaggregates #enzymes #Q10 #PLFA #GlobalWarming
If you are interested in the PLFA method and his interpretation, plese have a look at our recent perspective lead by a fantastic researcher, Stefan Gorka, and in collaboration with my dear colleagues @christinakaiser.bsky.social and Hannes Schmidt
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Soil bacterial neutral lipid fatty acids: Markers for carbon storage or necromass?
Intracellular carbon storage is a common strategy of soil microbes to cope with resource fluctuations. While fungal neutral lipid fatty acids (NLFAs) …
www.sciencedirect.com
June 1, 2025 at 7:33 PM
If you are interested in the PLFA method and his interpretation, plese have a look at our recent perspective lead by a fantastic researcher, Stefan Gorka, and in collaboration with my dear colleagues @christinakaiser.bsky.social and Hannes Schmidt
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Learn Lean 4 with PLFA (Programming Language Foundations in Agda) proofs. ~ rami3l. github.com/rami3l/plfl #ITP #LeanProver
April 7, 2025 at 8:55 AM
Learn Lean 4 with PLFA (Programming Language Foundations in Agda) proofs. ~ rami3l. github.com/rami3l/plfl #ITP #LeanProver
The study started in 2020 when we put together a great idea with @loutsi.bsky.social @joergschnecker.bsky.social @andreasrichter.bsky.social, to expand the vapor method to qSIP and deuterium in PLFA, sampling an incredible multifactorial experiment called ClimGrass
November 29, 2024 at 7:55 PM
The study started in 2020 when we put together a great idea with @loutsi.bsky.social @joergschnecker.bsky.social @andreasrichter.bsky.social, to expand the vapor method to qSIP and deuterium in PLFA, sampling an incredible multifactorial experiment called ClimGrass
地獄の素人ボイチェンおじさんラジオそのらじ！
今回はフリートークです。
https://www.youtube.com/live/SSrUo2-pLFA
おたより募集中！
投稿フォームはこちら！
https://forms.gle/ex79SZYSemgHoMAcA
#VRChat #そのらじ #VRC放送局
今回はフリートークです。
https://www.youtube.com/live/SSrUo2-pLFA
おたより募集中！
投稿フォームはこちら！
https://forms.gle/ex79SZYSemgHoMAcA
#VRChat #そのらじ #VRC放送局
そのらじAwaiSonoMidnightRadio - YouTube
そのらじｰAwaiSonoMidnightRadio- ＃328
www.youtube.com
May 1, 2026 at 8:51 AM
地獄の素人ボイチェンおじさんラジオそのらじ！
今回はフリートークです。
https://www.youtube.com/live/SSrUo2-pLFA
おたより募集中！
投稿フォームはこちら！
https://forms.gle/ex79SZYSemgHoMAcA
#VRChat #そのらじ #VRC放送局
今回はフリートークです。
https://www.youtube.com/live/SSrUo2-pLFA
おたより募集中！
投稿フォームはこちら！
https://forms.gle/ex79SZYSemgHoMAcA
#VRChat #そのらじ #VRC放送局
Were leading a new project on soil health funded by AHDB, working with SRUC 🌱 Exploring early indicators for farmers to boost confidence in monitoring soil. Key metrics: Soil Food Web, PLFA, and CO2 burst will help farmers assess soil health frequently and adapt practices. https://ow.ly/ilvp50XkYnR
Early indicators to monitor changes in soil health: evidence to update guidance for UK farmers - The Organic Research Centre
This project with AHDB, Organic Research Centre (ORC) and SRUC evaluates ten candidate soil health indicators for their suitability, evidence base and practical application to monitor soil health changes in UK farming systems. An interpretation framework for the top ranked indicators will be created
ow.ly
October 31, 2025 at 2:33 PM
Were leading a new project on soil health funded by AHDB, working with SRUC 🌱 Exploring early indicators for farmers to boost confidence in monitoring soil. Key metrics: Soil Food Web, PLFA, and CO2 burst will help farmers assess soil health frequently and adapt practices. https://ow.ly/ilvp50XkYnR
Main Responsibilities
• Conduct soil and plant sampling in field experiments
• Analyze microbial communities (amplicon sequencing, PLFA, enzyme assays)
• Investigate microbial traits and C/N cycling under drought
• Collaborate in data analysis and publication writing
• Conduct soil and plant sampling in field experiments
• Analyze microbial communities (amplicon sequencing, PLFA, enzyme assays)
• Investigate microbial traits and C/N cycling under drought
• Collaborate in data analysis and publication writing
June 3, 2025 at 11:58 AM
Main Responsibilities
• Conduct soil and plant sampling in field experiments
• Analyze microbial communities (amplicon sequencing, PLFA, enzyme assays)
• Investigate microbial traits and C/N cycling under drought
• Collaborate in data analysis and publication writing
• Conduct soil and plant sampling in field experiments
• Analyze microbial communities (amplicon sequencing, PLFA, enzyme assays)
• Investigate microbial traits and C/N cycling under drought
• Collaborate in data analysis and publication writing
After 2 months, I‘m back in the lab extracting fatty acids from various deep-sea organisms to assess their potential food sources and to better understand PLFA biosynthesis pathways when combining it with deuterium (stable isotope of hydrogen) enrichments.
February 12, 2025 at 1:52 PM
After 2 months, I‘m back in the lab extracting fatty acids from various deep-sea organisms to assess their potential food sources and to better understand PLFA biosynthesis pathways when combining it with deuterium (stable isotope of hydrogen) enrichments.
We updated existing conversion factors for PLFA and ergosterol biomarkers based on analyses in 30 soil fungal isolates and an updated literature review, and established one for fungal rDNA copy numbers - showing also the pros and cons of such an approach
April 12, 2024 at 12:27 PM
We updated existing conversion factors for PLFA and ergosterol biomarkers based on analyses in 30 soil fungal isolates and an updated literature review, and established one for fungal rDNA copy numbers - showing also the pros and cons of such an approach
New video on my YouTube channel: Crucified Barbara's Mia Karlsson at "Gamla Enskede Bryggeri" in Stockholm / Sweden (12.04.2025) - youtube.com/watch?v=PLfa... #MiaKarlsson #MiaColdheart #CrucifiedBarbara #RecordReleaseShow #Stockholm #Sweden #Sverige #JohnHiatt #JudasPriest @thecrucifier.bsky.social
Crucified Barbara's Mia Karlsson at "Gamla Enskede Bryggeri" in Stockholm / Sweden (12.04.2025)
YouTube video by Stefanie Nysand [Sound Kitchen]
youtube.com
April 22, 2025 at 11:13 AM
New video on my YouTube channel: Crucified Barbara's Mia Karlsson at "Gamla Enskede Bryggeri" in Stockholm / Sweden (12.04.2025) - youtube.com/watch?v=PLfa... #MiaKarlsson #MiaColdheart #CrucifiedBarbara #RecordReleaseShow #Stockholm #Sweden #Sverige #JohnHiatt #JudasPriest @thecrucifier.bsky.social
This latest paper instead allows us to quantify growth rates via PLFA, both at the community level and group specific. We found that whole community level and bacteria growth rates drop by half, while fungi do not decrease and even increase their growth rates
November 29, 2024 at 7:55 PM
This latest paper instead allows us to quantify growth rates via PLFA, both at the community level and group specific. We found that whole community level and bacteria growth rates drop by half, while fungi do not decrease and even increase their growth rates