#plfa
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bluitdebabel-bot.bsky.social
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October 26, 2026 at 11:06 PM
ucdavisplants.bsky.social
🌱 From Soil Biology and Biochemistry: Oak death reshaped deep-soil microbial functions within three years, potentially altering how savanna soils cycle carbon as tree mortality increases. (Anna C. Goodman, Ernest N. Walker, Glade D. Bogar, Laura M. Bogar)

▶️ www.sciencedirect.com/science/arti...
Fig. 4. Rolling averages of fungal biomass (ng PLFA/g soil) over depth by assigned functional group. Black points are measurements of fungal phospholipid fatty acids (PLFAs)/g soil, used here as a proxy for total fungal biomass. The black curve is a local regression connecting the mean fungal PLFA measurements at each depth. Other points represent each soil core's “imputed biomass” of fungi belonging to a given functional group (denoted by color). Imputed biomass is the proportion of the total fungal community (as described by ITS rRNA metabarcoding) assigned to a given functional group multiplied by the total fungal biomass as measured by PLFA. Functional groups were assigned to ASVs based on “primary lifestyle” denoted in the FungalTraits database and further agglomerated for visual simplicity. All local regressions were fitted using the “loess” method to geom_smooth() in ggplot2.
September 28, 2026 at 4:36 PM
uncited.org
Miikka B. Laine et al. demonstrate a refined phospholipid fatty acid (PLFA) analysis protocol that improves lipid separation and quantification in soil samples, revealing that freeze-dried storage at −80 °C preserves microbial community ratios better than −20 °C storage.
Soil Biology and Biochemistry
www.sciencedirect.com
September 26, 2026 at 6:22 AM
petrockette.bsky.social
How do you exist? I studied plfa and managed to show and complete most of the puzzles, but some eluded me.

I have never met a physics person who also loved dependent types.

I apologize if I am too enthusiastic but I am so glad you exist
September 20, 2026 at 3:18 AM
ulipink.bsky.social
man PLFA is so good
September 17, 2026 at 5:35 AM
joey.mathstodon.xyz.ap.brid.gy
Who's hiring functional programmers in 2026?

I've got a recent graduate who has been doing really good work on error messages in dependent types, who's taken both my PLAI and PLFA classes.

Sadly I haven't been at conferences for a while, so I don't know who's giving out shirts these days. Is […]
Original post on mathstodon.xyz
mathstodon.xyz
August 26, 2026 at 5:37 PM
stableisotopeecol.bsky.social
Liu, WS., Yao, S., Lin, ZJ. et al. A 13C-PLFA approach to assess the crop carbon allocation efficiency and arbuscular mycorrhizal assimilation capacity under conservation tillage. #stableisotopes Biol Fertil Soils 62, 25–37 (2026). doi.org/10.1007/s003...
Client Challenge
doi.org
January 13, 2026 at 7:16 AM
niooknaw.bsky.social
New publication: A global #database of #soil microbial phospholipid fatty #acids and #enzyme activities. #microbialcommunities
doi.org/10.1038/s415...
Figure 1 in Van Galen et al. (2025): "The PLFA and enzyme activity database contains 12,258 samples from 3,743 locations. The purple colour gradient shows the degree to which climatic space is represented by the samples (scale of 0 to 1, see Technical Validation section). Grey regions do not have sufficient climate data available to evaluate climatic representation. The histogram and donut plots show number of samples (in parentheses) from the PLFA (blue) and enzyme (red) datasets collected in different years, from different continents, from different biomes43, and within different categories of maximum sample depth. Only large segments of donut plots are labelled. Temp. = temperate."
October 9, 2025 at 8:59 AM
jpecol.bsky.social
Cancan Zhao et al. explored the patterns and mechanisms of how drought impacts nematode abundance and carbon footprint, microbial phospholipid fatty acid (PLFA) and heterotrophic soil respiration.
Details: doi.org/10.1093/jpe/...
January 31, 2025 at 8:31 PM
progressflorida.org
4 kick-ass state Representatives you should be following: @RepMarkPafford @RepRandolph @DwightBullard & @Kriseman #plfa
February 13, 2025 at 9:06 PM
andreasrichter.bsky.social
2/2
For this study, we developed a new method, #2H-vapor-PLFA-SIP, which labels soil with 2H-labelled water vapor, allowing to study the activity/growth of microbial communities without altering the soil moisture. Thanks to all involved!
#TER @cemess.bsky.social, @univie.ac.at vie.ac.at
November 29, 2024 at 6:40 PM
andreasrichter.bsky.social
Check out our new preprint
‚Soil fungi remain active and invest in storage compounds during drought independent of future climate conditions‘
by @loutsi.bsky.social, Alberto Canarini, and many more.
➡️2H-vapor-PLFA-SIP

biorxiv.org/cgi/content/...
October 24, 2023 at 4:39 PM
globalvisibility.bsky.social
telegra.ph/RAI-MOVIE-02...
RAI MOVIE
SOVICO https://headlines-world.com/advanced-search.html?lang=it&q=SOVICO PLFA II 2015 https://aepiot.com/advanced-search.html?lang=it&q=PLFA%20II%202015 20ª BRIGATA OPERATIVA LJUBART https://multi-sea...
telegra.ph
February 17, 2026 at 6:05 PM
mdpi.com.web.brid.gy
Nutrients, Vol. 17, Pages 821: Organ-Specific Responses to Chronic High-Fat Diets in Mice: Insights into Phospholipid Fatty Acid Distribution
Background/Objectives: This research aimed to investigate phospholipid fatty acid (PLFA) distribution in the brain, kidneys, and white adipose tissue (WAT) and lipid profiles in response to high-fat diets. Methods: Adult female C57BL/6 mice were fed high-fat diets containing 25% linseed, palm, or sunflower oil for 100 days. The fatty acid composition of dietary oils and tissue PL were analyzed using gas–liquid chromatography. Results: Linseed oil increased n-3 polyunsaturated fatty acids (PUFAs) with subsequent conversion into long-chain n-3 PUFAs in the brain and kidney PL, while only alpha-linolenic acid was elevated in WAT. Palm and sunflower oils resulted in unique PLFA distributions in the kidneys and WAT. Palm oil raised linoleic acid without conversion to pro-inflammatory n-6 PUFAs. Sunflower oil increased saturated palmitic acid, as opposed to the rise in monounsaturated oleic acid. Linseed oil also significantly improved lipid profiles, reducing LDL and increasing HDL levels while enhancing cardiovascular indices. Conclusions: This study demonstrates that dietary oils significantly impact organ-specific PLFA profiles, with linseed oil enriching brain and renal n-3 PUFAs, while palm and sunflower oils induce distinct modifications in the kidney and WAT. Moreover, linseed oil offers notable cardioprotective benefits due to the favorable lipid profile changes. These findings highlight the importance of dietary fat selection in achieving balanced lipid metabolism and suggest that diverse oil combinations may be essential for optimizing health outcomes.
www.mdpi.com
February 27, 2025 at 9:54 AM
albertocanarini.bsky.social
Main Responsibilities

• Conduct soil and plant sampling in field experiments
• Analyze microbial communities (amplicon sequencing, PLFA, enzyme assays)
• Investigate microbial traits and C/N cycling under drought
• Collaborate in data analysis and publication writing
June 3, 2025 at 11:58 AM
albertocanarini.bsky.social
If you are interested in the PLFA method and his interpretation, plese have a look at our recent perspective lead by a fantastic researcher, Stefan Gorka, and in collaboration with my dear colleagues @christinakaiser.bsky.social and Hannes Schmidt

www.sciencedirect.com/science/arti...
Soil bacterial neutral lipid fatty acids: Markers for carbon storage or necromass?
Intracellular carbon storage is a common strategy of soil microbes to cope with resource fluctuations. While fungal neutral lipid fatty acids (NLFAs) …
www.sciencedirect.com
June 1, 2025 at 7:33 PM
albertocanarini.bsky.social
This latest paper instead allows us to quantify growth rates via PLFA, both at the community level and group specific. We found that whole community level and bacteria growth rates drop by half, while fungi do not decrease and even increase their growth rates
November 29, 2024 at 7:55 PM
albertocanarini.bsky.social
The study started in 2020 when we put together a great idea with @loutsi.bsky.social @joergschnecker.bsky.social @andreasrichter.bsky.social, to expand the vapor method to qSIP and deuterium in PLFA, sampling an incredible multifactorial experiment called ClimGrass
November 29, 2024 at 7:55 PM
orlandocountysc.bsky.social
If you haven’t watched Unification yet it’s the best Trek in ages and will bring a tear to your eye if Kirk/Spock are your thing.

Shatner doing it after Paramount has phased him out was incredible.

youtu.be/_wbq2Y-PlfA?...
William Shatner Captain Kirt Leonard Nimoy Spock final time together complete 765874 Unification
YouTube video by rch4ran
youtu.be
November 26, 2024 at 6:08 PM
ykuzyakov.bsky.social
Bacterial necromass decomposition and #priming effects in #soils depend on #fertilization
NEW in @SoilBiolBiochem
doi.org/10.1016/j.so...

50-day Free: authors.elsevier.com/c/1lsrL_tPjA...

#SoilOrganicMatter #soil #primingeffect #CO2 #CH4 #microbialbiomass #PLFA #Fertilisers #soilbacteria #paddy
October 2, 2025 at 7:36 AM
ykuzyakov.bsky.social
💥NEW in @SoilBiolBiochem

Aggregates mediate stability & temperature sensitivity of #soil #carbon in response to #biochar & #straw amendments
doi.org/10.1016/j.so...

50-day Free: authors.elsevier.com/c/1lkOK8g13f...

#SoilOrganicMatter #soilaggregates #enzymes #Q10 #PLFA #GlobalWarming
September 8, 2025 at 6:25 AM
ykuzyakov.bsky.social
Final version in @plantandsoil
Mycorrhizal growth and resource exchange define coexistence of plants
doi.org/10.1007/s111...

#carbonsequestration #soil #agriculture #Roots #microbialbiomass #PLFA #Fertilisers #nitrogen #rhizosphere #13Clabeling #isotopes
May 30, 2025 at 12:40 PM
tcamenzind.bsky.social
We updated existing conversion factors for PLFA and ergosterol biomarkers based on analyses in 30 soil fungal isolates and an updated literature review, and established one for fungal rDNA copy numbers - showing also the pros and cons of such an approach
April 12, 2024 at 12:27 PM
qbane.me
Fully in-browser PLFA
July 29, 2026 at 12:19 PM
drtanjastratmann.bsky.social
After 2 months, I‘m back in the lab extracting fatty acids from various deep-sea organisms to assess their potential food sources and to better understand PLFA biosynthesis pathways when combining it with deuterium (stable isotope of hydrogen) enrichments.
February 12, 2025 at 1:52 PM