Air filtration is a commonly used biosecurity practice to minimize the spread of airborne diseases. Common airborne diseases include Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), Mycopl...

Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic losses in the global swine industry due to its high genetic diversity and different virulence levels, which complicate disease management and vaccine development. This study evaluated longitudinal changes in the immune cell composition of bronchoalveolar lavage fluid and the clinical outcomes across PRRSV strains with varying virulence, using techniques including single-cell transcriptomics. In highly virulent infection, faster viral replication results in an earlier peak lung-damage time point, marked by significant interstitial pneumonia, a significant decrease in macrophages, and an influx of lymphocytes. Viral tracking reveals less than 5% of macrophages are directly infected, and further analysis indicates bystander cell death, likely regulated by exosomal microRNAs as a significant factor. In contrast, the peak intermediate infection shows a delayed lung-damage time point with fewer cell popul

El estudio respalda el uso de métodos de muestreo menos invasivos para la vigilancia del PRRS como alternativas confiables y rentables para detectar el virus en cerdos ...

Author summary Hyperactivation of the NLRP3 inflammasome triggers excessive proinflammatory cytokine release, skewing the host response toward hyperinflammation and severe tissue damage. PRRSV-2, a po...

Porcine reproductive and respiratory syndrome virus (PRRSV), an Arteriviridae family enveloped RNA virus, is a major swine pathogen. Using yeast transformation-associated recombination (TAR) cloning, ...

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically significant viral pathogens affecting the global swine industry, yet the specific host factors that support ...

Ubiquitin-mediated proteasomal degradation is a central antiviral mechanism; however, how porcine reproductive and respiratory syndrome virus (PRRSV) counteracts this host defense has remained unclear...

Porcine reproductive and respiratory syndrome virus (PRRSV) remains a major challenge to global swine production due to its genetic diversity, rapid mutation rate, and ability to evade host immunity. ...

La vacuna intramuscular demostró no ser inferior a la vacuna intranasal en el alivio de los signos clínicos y contribuyó a reducir la pérdida de peso ...

The development of a PRRSV DIVA vaccine is crucial for effectively managing outbreaks in swine populations. DIVA vaccines allow differentiation between vaccinated and naturally infected animals, aidin...

Moloney leukemia virus 10 protein (MOV10), a DExD-box RNA helicase, is a known component of cytoplasmic RNA processing centers known as P bodies. As an interferon-stimulated gene (ISG), MOV10 exhibits broad-spectrum antiviral activity against multiple viruses, including SARS-CoV-2, HIV, HSV-1, IAV, and PRRSV. Here, we identify a novel helicase-independent antiviral mechanism of MOV10 against SADS-CoV. In this study, we show that MOV10 bridges TRIM24 and TRAF3 and facilitates K63-linked ubiquitination of TRAF3 independent of its helicase activity. This process strongly amplifies downstream interferon antiviral responses and suppresses viral replication. Furthermore, the SADS-CoV N protein efficiently antagonizes MOV10-mediated immune defense by disrupting the MOV10-TRAF3 interaction and inducing K48-linked ubiquitination and proteasomal degradation of TRAF3. Collectively, this study establishes a previously uncharacterized MOV10-TRIM24-TRAF3 antiviral regulatory axis, which offers new mechanistic insights into how coronaviruses evade host immune defenses.

La vacuna intramuscular demostró no ser inferior a la vacuna intranasal en el alivio de los signos clínicos y contribuyó a reducir la pérdida de peso ...

Author summary PRRSV poses a major threat to the global swine industry, and no effective vaccines or antiviral drugs have been developed. A deep understanding of the PRRSV replication mechanism is ess...

In 2023, Health Canada received a submission to allow the sale of genetically modified pigs which are resistant to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), called PRRSV-resistant p...

Today, Health Canada and the Canadian Food Inspection Agency (CFIA) announced the completion of independent, comprehensive safety assessments on pigs resistant to PRRSV following applications from Gen...

PRRS is one of the major diseases affecting the global swine industry. Infection with PRRSV can cause respiratory disease in pigs of all ages and reproductive disorders in sows. Therefore, understanding the interaction between PRRSV and host factors may help to develop new antiviral strategies against PRRSV. We found that PRRSV Nsp4 was important for nuclear export of TRIM28 in a CRM1-dependent manner during PRRSV infection. TRIM28 in the cytoplasm increases the formation of VPS34-Beclin1 complex by interacting with Vps34, further initiating autophagy. Hence, our study reveals a novel mechanism of PRRSV-mediated autophagy and provides valuable information for further understanding the pathogenesis of PRRS, which might contribute to the development of novel antiviral drugs.

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major swine pathogen, which can survive host antiviral immunity with various mechanisms. PRRSV infection induces macroautophagy/auto...