Explore the latest publications and results from the PICKED Project, showcasing advancements in personalized kidney care and groundbreaking research.

A kidney-conditioned urinary peptidomic ageing clock predicts all-cause mortality and age-related health outcomes, such as coronary artery disease, heart

Asymptomatic ovarian cancer evades detection, driving its high mortality. Li et al. develop ProMS+, a machine-learning model combining seven serum peptidomic markers with age, CA125, and HE4, which achieves high accuracy, outperforming conventional tests. Monte Carlo simulations across diverse risk settings support its potential for high-risk surveillance, pending prospective validation.

Background Post-acute sequelae of severe acute respiratory syndrome coronavirus 2-infection (PASC) is challenging to diagnose and treat, and its molecular pathophysiology remains unclear. Urinary pept...

Neuropeptides modulate a diverse range of physiological functions, including those associated with feeding. Post-translational modifications (PTMs) contribute significantly to the dynamic nature of neuropeptide isoforms, influencing their functional diversity. Mass spectrometry is the gold-standard analytical technique for peptidomic analyses and is complemented by computational methods for peptide identification; however, the computational search space becomes increasingly difficult to manage as more potential modifications are considered. Using innovative approaches capable of addressing the vast combinations of possible modifications, such as the PEAKS PTM search algorithm, we globally profiled the neuropeptidome ofCancer borealis(Jonah crab) to investigate the role of PTMs in feeding- and appetite-related processes over time. Through an in-depth examination of several notable modifications, we proposed PTM-associated motifs for neuropeptides, which may enhance future identification capabilities. Furthermore, this work revealed neuropeptides that were characteristically modified depending on the crab’s feeding status and time post-feeding, suggesting potential biological significance. This study represents the first large-scale investigation of the modified crustacean neuropeptidome, providing new insights into the regulatory implications of PTMs in biological systems.

Edible mushrooms are increasingly recognized due to their high-quality protein content and bioactive potential. This study evaluated pulsed electric f…

Edible mushrooms are increasingly recognized due to their high-quality protein content and bioactive potential. This study evaluated pulsed electric f…

The Ndel1 protein is crucial for neurodevelopment, but its role in the brain peptidome is unknown. Here we compared the peptidome of Ndel1 conditional knockout (Ndel1_cKO) mice with controls (CTRL) i...

Severe acute pancreatitis (SAP) involves dynamic shifts from inflammation to immunosuppression, where peptidomic profile evolution may reveal prognostic biomarkers. Here, the plasma peptidome of rat...

Scientific Reports - Mass spectrometry-based serum peptidomic profiling reveals potential biomarker for canine hepatozoonosis

This study reveals a complex peptidomic landscape in the mouse somatosensory cortex, identifying locally released and potentially transported neuropeptides using a membrane-free nanodialysis sampling...

Caprine and bovine milk have been widely used as a supplementary nutritional source during early infancy. This study established an integrated peptido…

Artificial tiger bone powder (ATBP) is an animal-derived traditional Chinese medicine for treating osteoporosis. The combination of NanoLC-HRMS and UHPLC-QQQ-MS was used to identify and quantify the ...

Rhaebo guttatus is a South American frog species from the Bufonidae family. Its defense against predators is primarily attributed to small molecules s…

The precision-cut lung slice (PCLS) model is an ex vivo tissue system that has been used to model disease and examine the effects of exogenous compoun…