Our paper is finally out: Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier | Science Advances www.science.org/doi/10.1126/...
#mitochondria #cryo-EM
#mitochondria #cryo-EM
Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier
The mitochondrial pyruvate carrier transport mechanism is ΔpH driven and is inhibited competitively by distinct compound classes.
www.science.org
April 18, 2025 at 6:16 PM
Our paper is finally out: Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier | Science Advances www.science.org/doi/10.1126/...
#mitochondria #cryo-EM
#mitochondria #cryo-EM
Mitochondrial pyruvate transport regulates presynaptic metabolism and neurotransmission
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Mitochondrial pyruvate transport regulates presynaptic metabolism and neurotransmission
The brain uses pyruvate as an energy source, and mitochondrial pyruvate metabolism in nerve terminals controls neurotransmission.
www.science.org
November 20, 2024 at 1:31 PM
Mitochondrial pyruvate transport regulates presynaptic metabolism and neurotransmission
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
#Thiamine in the ICU: 10 high-yield pearls
1. Unexplained lactate? Think thiamine
T is required for pyruvate dehydrogenase. Deficiency blocks conversion of pyruvate to acetyl-CoA, shunting pyruvate toward lactate. Thus, T deficiency belongs in the diff dx for otherwise unexplained lactic acidosis
1. Unexplained lactate? Think thiamine
T is required for pyruvate dehydrogenase. Deficiency blocks conversion of pyruvate to acetyl-CoA, shunting pyruvate toward lactate. Thus, T deficiency belongs in the diff dx for otherwise unexplained lactic acidosis
September 20, 2026 at 7:49 PM
#Thiamine in the ICU: 10 high-yield pearls
1. Unexplained lactate? Think thiamine
T is required for pyruvate dehydrogenase. Deficiency blocks conversion of pyruvate to acetyl-CoA, shunting pyruvate toward lactate. Thus, T deficiency belongs in the diff dx for otherwise unexplained lactic acidosis
1. Unexplained lactate? Think thiamine
T is required for pyruvate dehydrogenase. Deficiency blocks conversion of pyruvate to acetyl-CoA, shunting pyruvate toward lactate. Thus, T deficiency belongs in the diff dx for otherwise unexplained lactic acidosis
Interesting new research from Antoine Marçais’ lab showing a central role for pyruvate and the Citrate-Malate Shuttle (non-canonical TCA cycle) in human NK cells.
#immunology #NKcells #Immunometabolism
doi.org/10.1038/s422...
See our commentary article here:
www.nature.com/articles/s42...
#immunology #NKcells #Immunometabolism
doi.org/10.1038/s422...
See our commentary article here:
www.nature.com/articles/s42...
Natural killer loops: pyruvate in, lactate out - Nature Metabolism
Resting human CD56Dim natural killer cells utilize exogenous pyruvate to support energy metabolism. A new study shows that these cells take up exogenous pyruvate, which regenerates cytosolic NAD+ via ...
www.nature.com
January 3, 2025 at 12:30 PM
Interesting new research from Antoine Marçais’ lab showing a central role for pyruvate and the Citrate-Malate Shuttle (non-canonical TCA cycle) in human NK cells.
#immunology #NKcells #Immunometabolism
doi.org/10.1038/s422...
See our commentary article here:
www.nature.com/articles/s42...
#immunology #NKcells #Immunometabolism
doi.org/10.1038/s422...
See our commentary article here:
www.nature.com/articles/s42...
Structure of human mitochondrial pyruvate carrier - what a milestone by the wonderful Dan Ma's lab from Westlake! Using Xudong Wu's expertise in nanoantibodies, they capture MPC during transport, revealing its pseudo-symmetric model and alternate opening mechanism with functional cardiolipin. Bravo!
Structures and mechanism of human mitochondrial pyruvate carrier - Nature
Nature - Structures and mechanism of human mitochondrial pyruvate carrier
www.nature.com
March 18, 2025 at 11:27 PM
Structure of human mitochondrial pyruvate carrier - what a milestone by the wonderful Dan Ma's lab from Westlake! Using Xudong Wu's expertise in nanoantibodies, they capture MPC during transport, revealing its pseudo-symmetric model and alternate opening mechanism with functional cardiolipin. Bravo!
New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...
High-performance genetically-encoded green and red fluorescent biosensors for pyruvate
Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F > 10) single fluorescent protein (FP)-base...
www.biorxiv.org
April 19, 2025 at 6:42 AM
New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...
Intracellular metabolic gradients dictate dependence on exogenous pyruvate
www.nature.com/articles/s42...
Congrats to @lydiafinley.bsky.social Lab!
www.nature.com/articles/s42...
Congrats to @lydiafinley.bsky.social Lab!
Intracellular metabolic gradients dictate dependence on exogenous pyruvate - Nature Metabolism
Jackson et al. provide insight into how metabolic adaptations that accompany cell state transitions drive reliance on exogenous nutrient availability, focusing on pyruvate as a key metabolite in centr...
www.nature.com
April 29, 2025 at 5:42 AM
Intracellular metabolic gradients dictate dependence on exogenous pyruvate
www.nature.com/articles/s42...
Congrats to @lydiafinley.bsky.social Lab!
www.nature.com/articles/s42...
Congrats to @lydiafinley.bsky.social Lab!
Structure of mitochondrial pyruvate carrier and its inhibition mechanism
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structure of mitochondrial pyruvate carrier and its inhibition mechanism - Nature
Structural findings define the architecture of the mitochondrial pyruvate carrier, delineate its substrate-binding site and translocation pathway, and reveal its major conformational states, providing...
www.nature.com
March 5, 2025 at 5:43 PM
Structure of mitochondrial pyruvate carrier and its inhibition mechanism
www.nature.com/articles/s41...
www.nature.com/articles/s41...
#compchem #compchemsky Just published in Chem. Eur. J.: "High-resolution Molecular Dynamics Simulations of the Pyruvate Kinase Muscle Isoform 1 and 2 (PKM1/2)."
chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Nice work by Q. Delobelle & great collab with V. Maréchal.
High‐resolution Molecular Dynamics Simulations of the Pyruvate Kinase Muscle Isoform 1 and 2 (PKM1/2).
Glucose metabolism plays a pivotal role in physiological processes and cancer growth. The final stage of glycolysis, converting phosphoenolpyruvate (PEP) into pyruvate, is catalyzed by the pyruvate k...
chemistry-europe.onlinelibrary.wiley.com
December 1, 2024 at 1:01 PM
#compchem #compchemsky Just published in Chem. Eur. J.: "High-resolution Molecular Dynamics Simulations of the Pyruvate Kinase Muscle Isoform 1 and 2 (PKM1/2)."
chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Nice work by Q. Delobelle & great collab with V. Maréchal.
Mitochondrial pyruvate transport regulates presynaptic metabolism and neurotransmission
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Mitochondrial pyruvate transport regulates presynaptic metabolism and neurotransmission
The brain uses pyruvate as an energy source, and mitochondrial pyruvate metabolism in nerve terminals controls neurotransmission.
www.science.org
November 19, 2024 at 6:18 PM
Mitochondrial pyruvate transport regulates presynaptic metabolism and neurotransmission
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier
Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier
The mitochondrial pyruvate carrier transport mechanism is ΔpH driven and is inhibited competitively by distinct compound classes.
www.science.org
April 18, 2025 at 11:20 PM
Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier
Thanks! Preprint is here: doi.org/10.1101/2025...
September 8, 2025 at 10:59 PM
Thanks! Preprint is here: doi.org/10.1101/2025...
New Letter: "Rubisco supplies pyruvate for the 2-C-methyl-d-erythritol-4-phosphate pathway" rdcu.be/dVZMj
Rubisco fixes CO2. Its lesser-known side job is supplying pyruvate in the chloroplast, an observation that solves a long-standing paradox of central metabolism. #PlantScience
Rubisco fixes CO2. Its lesser-known side job is supplying pyruvate in the chloroplast, an observation that solves a long-standing paradox of central metabolism. #PlantScience
October 4, 2024 at 10:19 AM
New Letter: "Rubisco supplies pyruvate for the 2-C-methyl-d-erythritol-4-phosphate pathway" rdcu.be/dVZMj
Rubisco fixes CO2. Its lesser-known side job is supplying pyruvate in the chloroplast, an observation that solves a long-standing paradox of central metabolism. #PlantScience
Rubisco fixes CO2. Its lesser-known side job is supplying pyruvate in the chloroplast, an observation that solves a long-standing paradox of central metabolism. #PlantScience
@avrahamcoopermd.bsky.social why nor share w/ your followers here on 🔵bsky your 𝕏eet from Dec 26, 2022 ?
"Who knew that the Pyruvate Dehydrogenase Complex actually resembles a pub brawl?"
we love it! 😄
"Who knew that the Pyruvate Dehydrogenase Complex actually resembles a pub brawl?"
we love it! 😄
December 5, 2024 at 2:03 PM
@avrahamcoopermd.bsky.social why nor share w/ your followers here on 🔵bsky your 𝕏eet from Dec 26, 2022 ?
"Who knew that the Pyruvate Dehydrogenase Complex actually resembles a pub brawl?"
we love it! 😄
"Who knew that the Pyruvate Dehydrogenase Complex actually resembles a pub brawl?"
we love it! 😄
How do brain cells survive when glucose runs low? 🤔 Pyruvate to the rescue! New research in
@ScienceAdvances
shows how this fuel source steps up to protect neurons. #mitochondria #metabolism #neurons
www.science.org/doi/10.1126/...
@ScienceAdvances
shows how this fuel source steps up to protect neurons. #mitochondria #metabolism #neurons
www.science.org/doi/10.1126/...
Mitochondrial pyruvate transport regulates presynaptic metabolism and neurotransmission
The brain uses pyruvate as an energy source, and mitochondrial pyruvate metabolism in nerve terminals controls neurotransmission.
www.science.org
November 19, 2024 at 4:53 PM
How do brain cells survive when glucose runs low? 🤔 Pyruvate to the rescue! New research in
@ScienceAdvances
shows how this fuel source steps up to protect neurons. #mitochondria #metabolism #neurons
www.science.org/doi/10.1126/...
@ScienceAdvances
shows how this fuel source steps up to protect neurons. #mitochondria #metabolism #neurons
www.science.org/doi/10.1126/...
Boost your metabolism (research) with 🍎🥧 pyruvate biosensors, now available on Addgene! Green ones and red ones, with a range of affinities. www.addgene.org/browse/artic...
Addgene: High-performance genetically-encoded green and red fluorescent biosensors for pyruvate
www.addgene.org
June 25, 2025 at 5:21 AM
Boost your metabolism (research) with 🍎🥧 pyruvate biosensors, now available on Addgene! Green ones and red ones, with a range of affinities. www.addgene.org/browse/artic...
Proposed pH-dependent mechanism of the mitochondrial pyruvate carrier. In the outward-open state, positively charged K49 and H86 bind pyruvate, initiating conformational changes to the inward-open state. The high matrix pH deprotonates H86, allowing pyruvate to leave.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
April 18, 2025 at 10:02 PM
Proposed pH-dependent mechanism of the mitochondrial pyruvate carrier. In the outward-open state, positively charged K49 and H86 bind pyruvate, initiating conformational changes to the inward-open state. The high matrix pH deprotonates H86, allowing pyruvate to leave.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
I'm excited to share our latest story from the
@lydiafinley.bsky.social lab, where we show that it's not just whether cells take up nutrients that matters, but how and where those metabolites are used. Out now in @naturemetabolism.bsky.social: rdcu.be/ejFTs 1/
@lydiafinley.bsky.social lab, where we show that it's not just whether cells take up nutrients that matters, but how and where those metabolites are used. Out now in @naturemetabolism.bsky.social: rdcu.be/ejFTs 1/
Intracellular metabolic gradients dictate dependence on exogenous pyruvate
Nature Metabolism - Jackson et al. provide insight into how metabolic adaptations that accompany cell state transitions drive reliance on exogenous nutrient availability, focusing on pyruvate as a...
rdcu.be
April 28, 2025 at 2:28 PM
I'm excited to share our latest story from the
@lydiafinley.bsky.social lab, where we show that it's not just whether cells take up nutrients that matters, but how and where those metabolites are used. Out now in @naturemetabolism.bsky.social: rdcu.be/ejFTs 1/
@lydiafinley.bsky.social lab, where we show that it's not just whether cells take up nutrients that matters, but how and where those metabolites are used. Out now in @naturemetabolism.bsky.social: rdcu.be/ejFTs 1/
This ‘fundamental’ study uncovers how directing pyruvate into mitochondria can shrink cells by shifting their metabolism away from building amino acids and proteins.
buff.ly/hFHjKy9
buff.ly/hFHjKy9
December 27, 2025 at 11:01 AM
This ‘fundamental’ study uncovers how directing pyruvate into mitochondria can shrink cells by shifting their metabolism away from building amino acids and proteins.
buff.ly/hFHjKy9
buff.ly/hFHjKy9
Regulation of Pyruvate Dehydrogenase Kinase (Pdk4) offers mechanistic insights into enhancing stemness of aging intestinal stem cells via switching metabolic fuel preference and increased mitochondrial oxygen consumption rate
www.nature.com/articles/s42...
www.nature.com/articles/s42...
Mitochondrial oxidation of carbohydrate fuel driven by pyruvate dehydrogenase rescues stemness of aging intestinal stem cells - Communications Biology
Regulation of Pyruvate Dehydrogenase Kinase (Pdk4) offers mechanistic insights into enhancing stemness of aging intestinal stem cells via switching metabolic fuel preference and increased mitochondria...
www.nature.com
September 23, 2026 at 12:58 PM
Regulation of Pyruvate Dehydrogenase Kinase (Pdk4) offers mechanistic insights into enhancing stemness of aging intestinal stem cells via switching metabolic fuel preference and increased mitochondrial oxygen consumption rate
www.nature.com/articles/s42...
www.nature.com/articles/s42...
This ‘fundamental’ study uncovers how directing pyruvate into mitochondria can shrink cells by shifting their metabolism away from building amino acids and proteins.
buff.ly/hFHjKy9
buff.ly/hFHjKy9
November 26, 2025 at 4:15 PM
This ‘fundamental’ study uncovers how directing pyruvate into mitochondria can shrink cells by shifting their metabolism away from building amino acids and proteins.
buff.ly/hFHjKy9
buff.ly/hFHjKy9
Kayoko (blue Archive) By - Pyruvate
#feet #soles #toes #legs #girl #art #anime
#feet #soles #toes #legs #girl #art #anime
November 28, 2025 at 2:43 PM
Kayoko (blue Archive) By - Pyruvate
#feet #soles #toes #legs #girl #art #anime
#feet #soles #toes #legs #girl #art #anime