Por que odiamos? Ep 6: Microsoft
YouTube video by Tecnologia e Classe (TeClas)
www.youtube.com
April 1, 2026 at 2:39 PM
RCMC Registration – Easy & Hassle-Free Process
RCMC Registration helps exporters get recognition from the relevant Export Promotion Council.
It is useful for exporters to access export benefits and support.
Visit - www.compliancecalendar.in/rcmc-registr...
RCMC Registration helps exporters get recognition from the relevant Export Promotion Council.
It is useful for exporters to access export benefits and support.
Visit - www.compliancecalendar.in/rcmc-registr...
DGFT/Registration cum Membership Certificate (RCMC) Registration
RCMC stands for Registration-Cum-Membership Certificate, validating exporters dealing with products registered with authorized agencies.
www.compliancecalendar.in
September 30, 2026 at 7:34 AM
RCMC Registration – Easy & Hassle-Free Process
RCMC Registration helps exporters get recognition from the relevant Export Promotion Council.
It is useful for exporters to access export benefits and support.
Visit - www.compliancecalendar.in/rcmc-registr...
RCMC Registration helps exporters get recognition from the relevant Export Promotion Council.
It is useful for exporters to access export benefits and support.
Visit - www.compliancecalendar.in/rcmc-registr...
Very nice new paper from Paggi, Long, Zhang using physical modeling of RCMC data to reconstruct physical structures of euchromatin at the nucleosome scale:
Euchromatin forms condensed domains and E-P interactions largely occur on the surface!
www.nature.com/articles/s41...
Euchromatin forms condensed domains and E-P interactions largely occur on the surface!
www.nature.com/articles/s41...
September 28, 2026 at 1:49 PM
Very nice new paper from Paggi, Long, Zhang using physical modeling of RCMC data to reconstruct physical structures of euchromatin at the nucleosome scale:
Euchromatin forms condensed domains and E-P interactions largely occur on the surface!
www.nature.com/articles/s41...
Euchromatin forms condensed domains and E-P interactions largely occur on the surface!
www.nature.com/articles/s41...
Masahiro and I were fortunate to contribute some RCMC analyses to this beautiful paper from Koska and Wysocka that comprehensively dissects the determinants of promoter competition:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
July 22, 2026 at 11:32 PM
Masahiro and I were fortunate to contribute some RCMC analyses to this beautiful paper from Koska and Wysocka that comprehensively dissects the determinants of promoter competition:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
(1/n) New preprint from @claricehongky.bsky.social
Fan Fang Varshini Ramanathan in collab w Jie Liu.
Q: How do we get ultra-high-res 3D genome maps?
A: New deep learning model, Cleopatra.
Cleo trains on Micro-C, fine-tunes on RCMC, and predicts genome-wide 3D maps at ultra-high resolution.
Fan Fang Varshini Ramanathan in collab w Jie Liu.
Q: How do we get ultra-high-res 3D genome maps?
A: New deep learning model, Cleopatra.
Cleo trains on Micro-C, fine-tunes on RCMC, and predicts genome-wide 3D maps at ultra-high resolution.
May 7, 2025 at 8:53 PM
(1/n) New preprint from @claricehongky.bsky.social
Fan Fang Varshini Ramanathan in collab w Jie Liu.
Q: How do we get ultra-high-res 3D genome maps?
A: New deep learning model, Cleopatra.
Cleo trains on Micro-C, fine-tunes on RCMC, and predicts genome-wide 3D maps at ultra-high resolution.
Fan Fang Varshini Ramanathan in collab w Jie Liu.
Q: How do we get ultra-high-res 3D genome maps?
A: New deep learning model, Cleopatra.
Cleo trains on Micro-C, fine-tunes on RCMC, and predicts genome-wide 3D maps at ultra-high resolution.
Jamie Drayton and I were fortunate to contribute some RCMC analyses to this beautiful paper from Eder, Moene...van Steensel that systematically maps the relationship between enhancer location and gene expression (and nice to see RCMC predict expression in Fig 2H-I):
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
September 19, 2025 at 7:41 PM
Jamie Drayton and I were fortunate to contribute some RCMC analyses to this beautiful paper from Eder, Moene...van Steensel that systematically maps the relationship between enhancer location and gene expression (and nice to see RCMC predict expression in Fig 2H-I):
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
(5/n) ESTIMATE 1:
- Expression scales linearly with RCMC-measured E-P loop strength
- Linearity only if R(RCMC) [RCMC capture radius] equals R(E-P) [func E-P interaction radius]
- Thus, R(E-P) ~ R(RCMC) ~ 25-42 nm
- Expression scales linearly with RCMC-measured E-P loop strength
- Linearity only if R(RCMC) [RCMC capture radius] equals R(E-P) [func E-P interaction radius]
- Thus, R(E-P) ~ R(RCMC) ~ 25-42 nm
June 22, 2026 at 4:05 PM
(5/n) ESTIMATE 1:
- Expression scales linearly with RCMC-measured E-P loop strength
- Linearity only if R(RCMC) [RCMC capture radius] equals R(E-P) [func E-P interaction radius]
- Thus, R(E-P) ~ R(RCMC) ~ 25-42 nm
- Expression scales linearly with RCMC-measured E-P loop strength
- Linearity only if R(RCMC) [RCMC capture radius] equals R(E-P) [func E-P interaction radius]
- Thus, R(E-P) ~ R(RCMC) ~ 25-42 nm
New preprint with Cerda-Smith, Hutchinson, Eyler, Wood et. al combining high-res Region Capture Micro-C (RCMC) with CRISPRa screens to identify functional enhancers of tumor supressor, PTEN, revealing separation between structural and functional elements:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
September 23, 2023 at 5:25 PM
New preprint with Cerda-Smith, Hutchinson, Eyler, Wood et. al combining high-res Region Capture Micro-C (RCMC) with CRISPRa screens to identify functional enhancers of tumor supressor, PTEN, revealing separation between structural and functional elements:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
New Article! Mapping 3D genome organization at nucleosome-scale with Micro-C and Region Capture Micro-C (RCMC)
Mapping 3D genome organization at nucleosome-scale with Micro-C and Region Capture Micro-C (RCMC)
Nature Protocols, Published online: 13 July 2026; doi:10.1038/s41596-026-01393-3This protocol for Micro-C achieves nucleosome-scale resolution of 3D genome organization and includes a further modification, Region Capture Micro-C (RCMC), that can provide even greater depth and the identification of even more finely resolved interactions.
dlvr.it
July 13, 2026 at 4:37 PM
New Article! Mapping 3D genome organization at nucleosome-scale with Micro-C and Region Capture Micro-C (RCMC)
(3/n) We engineering a synthetic 339 kb E-P pair in 'vacant' region
- Vary E-P dist
- +/- CTCF at E and P
- See nice dot in RCMC maps
- Can track E-P 3D distance and nascent RNA from 2Hz to 6+ hours using Super-Res Live Cell Imaging
- Txn strictly dependent on E.
- New DNA fluor label: synBsr1 (!)
- Vary E-P dist
- +/- CTCF at E and P
- See nice dot in RCMC maps
- Can track E-P 3D distance and nascent RNA from 2Hz to 6+ hours using Super-Res Live Cell Imaging
- Txn strictly dependent on E.
- New DNA fluor label: synBsr1 (!)
June 22, 2026 at 3:55 PM
(3/n) We engineering a synthetic 339 kb E-P pair in 'vacant' region
- Vary E-P dist
- +/- CTCF at E and P
- See nice dot in RCMC maps
- Can track E-P 3D distance and nascent RNA from 2Hz to 6+ hours using Super-Res Live Cell Imaging
- Txn strictly dependent on E.
- New DNA fluor label: synBsr1 (!)
- Vary E-P dist
- +/- CTCF at E and P
- See nice dot in RCMC maps
- Can track E-P 3D distance and nascent RNA from 2Hz to 6+ hours using Super-Res Live Cell Imaging
- Txn strictly dependent on E.
- New DNA fluor label: synBsr1 (!)
Congratulations, this is really interesting!
How well does human-trained Cleopatra predict mouse ESC micro-C & RCMC?
Inconsistencies might reveal interesting species-specific mechanisms?
Not that it matters but yes, this is also bc I would personally love to browse mESC data 😇
How well does human-trained Cleopatra predict mouse ESC micro-C & RCMC?
Inconsistencies might reveal interesting species-specific mechanisms?
Not that it matters but yes, this is also bc I would personally love to browse mESC data 😇
May 8, 2025 at 3:25 AM
Congratulations, this is really interesting!
How well does human-trained Cleopatra predict mouse ESC micro-C & RCMC?
Inconsistencies might reveal interesting species-specific mechanisms?
Not that it matters but yes, this is also bc I would personally love to browse mESC data 😇
How well does human-trained Cleopatra predict mouse ESC micro-C & RCMC?
Inconsistencies might reveal interesting species-specific mechanisms?
Not that it matters but yes, this is also bc I would personally love to browse mESC data 😇
VAN HALEN – The “Hot For Teacher” Story: “ALEX VAN HALEN Fooled Everyone” (Video) www.youtube.com/watch?v=Rcmc... bravewords.com/news/van-hal...
Alex Van Halen Fooled Everyone With This Drum Intro
YouTube video by Drumeo
www.youtube.com
June 9, 2026 at 1:28 AM
VAN HALEN – The “Hot For Teacher” Story: “ALEX VAN HALEN Fooled Everyone” (Video) www.youtube.com/watch?v=Rcmc... bravewords.com/news/van-hal...
Por que odiamos? Ep 6: Microsoft
YouTube video by Tecnologia e Classe (TeClas)
www.youtube.com
April 1, 2026 at 10:17 PM
Lots of thoughts on this:
1. I think the (RC)-micro-C protocol has similar coverage bias to Hi-C maybe a little worse (not like in that figure - see attached example).
2. The Dovetail protocol gives few informative reads (and is being discontinued I believe - can share more offline)
1. I think the (RC)-micro-C protocol has similar coverage bias to Hi-C maybe a little worse (not like in that figure - see attached example).
2. The Dovetail protocol gives few informative reads (and is being discontinued I believe - can share more offline)
November 23, 2024 at 11:05 PM
Lots of thoughts on this:
1. I think the (RC)-micro-C protocol has similar coverage bias to Hi-C maybe a little worse (not like in that figure - see attached example).
2. The Dovetail protocol gives few informative reads (and is being discontinued I believe - can share more offline)
1. I think the (RC)-micro-C protocol has similar coverage bias to Hi-C maybe a little worse (not like in that figure - see attached example).
2. The Dovetail protocol gives few informative reads (and is being discontinued I believe - can share more offline)
Is "Hot For Teacher" the greatest song ever recorded? Evidence continues to mount.
www.youtube.com/watch?v=Rcmc...
www.youtube.com/watch?v=Rcmc...
Alex Van Halen Fooled Everyone With This Drum Intro
YouTube video by Drumeo
www.youtube.com
June 5, 2026 at 3:58 PM
Is "Hot For Teacher" the greatest song ever recorded? Evidence continues to mount.
www.youtube.com/watch?v=Rcmc...
www.youtube.com/watch?v=Rcmc...
(2/n) Problem: Human genome is big --> Experimental genome-wide 3D mapping at nuc res costs ~$280,000,000
RCMC allows ultra-high res mapping a Mb-sized regions (nature.com/articles/s41...)
Idea: can we use deep learning to scale RCMC resolution 3D maps to the entire genome without paying $280M?
RCMC allows ultra-high res mapping a Mb-sized regions (nature.com/articles/s41...)
Idea: can we use deep learning to scale RCMC resolution 3D maps to the entire genome without paying $280M?
May 7, 2025 at 11:02 PM
(2/n) Problem: Human genome is big --> Experimental genome-wide 3D mapping at nuc res costs ~$280,000,000
RCMC allows ultra-high res mapping a Mb-sized regions (nature.com/articles/s41...)
Idea: can we use deep learning to scale RCMC resolution 3D maps to the entire genome without paying $280M?
RCMC allows ultra-high res mapping a Mb-sized regions (nature.com/articles/s41...)
Idea: can we use deep learning to scale RCMC resolution 3D maps to the entire genome without paying $280M?
That's a nice idea, we actually haven't tried to run human-trained Cleopatra on mESCs, but it would be interesting to do.
For the human RCMC we have ca. 44Mb per cell type, but for mESCs there is only ca. 7Mb in www.nature.com/articles/s41... so may be too little but we should try it on mESCs :)
For the human RCMC we have ca. 44Mb per cell type, but for mESCs there is only ca. 7Mb in www.nature.com/articles/s41... so may be too little but we should try it on mESCs :)
Region Capture Micro-C reveals coalescence of enhancers and promoters into nested microcompartments - Nature Genetics
Region Capture Micro-C (RCMC) combines MNase-based 3C with a tiling region-capture method. Profiling mouse embryonic stem cells with RCMC identifies nested microcompartments, which connect enhancers a...
www.nature.com
May 8, 2025 at 10:54 AM
That's a nice idea, we actually haven't tried to run human-trained Cleopatra on mESCs, but it would be interesting to do.
For the human RCMC we have ca. 44Mb per cell type, but for mESCs there is only ca. 7Mb in www.nature.com/articles/s41... so may be too little but we should try it on mESCs :)
For the human RCMC we have ca. 44Mb per cell type, but for mESCs there is only ca. 7Mb in www.nature.com/articles/s41... so may be too little but we should try it on mESCs :)
8/ Couldn't have done this without standing on great prior work, especially from David Hendrickson's lab and @dimplenotani.bsky.social's lab. Methods like SCEPTRE (Eugene Katsevich), RCMC (@andersshansen.bsky.social), ChromBPNet (@anshulkundaje.bsky.social) also really helped propel this project.
July 29, 2026 at 2:37 PM
8/ Couldn't have done this without standing on great prior work, especially from David Hendrickson's lab and @dimplenotani.bsky.social's lab. Methods like SCEPTRE (Eugene Katsevich), RCMC (@andersshansen.bsky.social), ChromBPNet (@anshulkundaje.bsky.social) also really helped propel this project.
We have generated a "mESC mega merge" Micro-C dataset with 54 Billion (!) unique ligations.
This map is freely available on GEO.
We have also annotated 65,929 consensus loops in mESCs (CTCF, E, P, other) available as a table.
The 54B Micro-C map approaches RCMC-resolution genome-wide!
This map is freely available on GEO.
We have also annotated 65,929 consensus loops in mESCs (CTCF, E, P, other) available as a table.
The 54B Micro-C map approaches RCMC-resolution genome-wide!
June 30, 2026 at 12:45 PM
We have generated a "mESC mega merge" Micro-C dataset with 54 Billion (!) unique ligations.
This map is freely available on GEO.
We have also annotated 65,929 consensus loops in mESCs (CTCF, E, P, other) available as a table.
The 54B Micro-C map approaches RCMC-resolution genome-wide!
This map is freely available on GEO.
We have also annotated 65,929 consensus loops in mESCs (CTCF, E, P, other) available as a table.
The 54B Micro-C map approaches RCMC-resolution genome-wide!
🙏Grateful to António Guterres for honouring Rohingya cultural heritage. This visit sends a powerful message: Rohingya lives matter; their culture, stories & hope for return prevail!
🗣️ Artisans at IOM's RCMC: Your visit gives us hope that we will not be forgotten.
🗣️ Artisans at IOM's RCMC: Your visit gives us hope that we will not be forgotten.
March 18, 2025 at 5:40 AM
🙏Grateful to António Guterres for honouring Rohingya cultural heritage. This visit sends a powerful message: Rohingya lives matter; their culture, stories & hope for return prevail!
🗣️ Artisans at IOM's RCMC: Your visit gives us hope that we will not be forgotten.
🗣️ Artisans at IOM's RCMC: Your visit gives us hope that we will not be forgotten.
