#RNAscope
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zandrea.bsky.social
Granulomas from untreated SIV/Mtb animals 1) had higher bacterial burden (colony-forming units, CFU) than Mtb only or SIV/ART/Mtb animals and 2) often had high SIV RNA levels from infected CD4 T cells and macrophages.
(Left) Total Mtb CFU in each granuloma isolated at necropsy. All granulomas harvested are shown, with dark shapes indicating the granulomas used for GeoMX. (Right) SIV RNA copies per 1 × 106 CD4 RNA copies in lung granulomas at necropsy. Each circle is a granuloma; horizontal lines indicate medians for each animal. All granulomas harvested are shown, with dark symbols indicating the granulomas used for GeoMX. RNAscope (SIV gag and pol; yellow) and immunohistochemistry (CD3+ T cells, green; CD11c/CD68+ myeloid cells, magenta) staining in SIV/Mtb (left) and SIV/ART/Mtb (right) granulomas. Scale bars: 100 μm.
November 11, 2026 at 4:39 PM
lazearlab.bsky.social
Margaret Dedloff's work out now in Science Advances!
IFN-L signaling to maternal DCs protects against congenital ZIKV infection:
www.science.org/doi/10.1126/...
@mdedloff.bsky.social @joelrc.bsky.social @celeste-robles.bsky.social @becca-casazza.bsky.social @michelleitano.bsky.social @science.org
Interferon lambda signaling to maternal dendritic cells protects against congenital Zika virus infection. Dedloff MR, Reinhardt TR, Ropp TF, Rivera-Cardona J, Robles CR, Casazza RL, Ezzell JA, Itano MS, Lazear HM. 2026. Science Advances. IFNAR KO and IFNAR IFNLR DKO pregnant dams were infected with ZIKV; dams lacking IFN-L signaling had fewer uterine DCs as assessed by flow cytometry and RNAscope. Conditional KO mice lacking IFN-L signaling globally, in myeloid cells, or in dendritic cells (but not neutrophils) had greater ZIKV congenital infection compared to WT dams. These results identify maternal dendritic cells as key IFN-L responsive cell types mediating protection against ZIKV congenital infection. https://www.science.org/doi/10.1126/sciadv.aeh1073
October 27, 2026 at 12:01 PM
lazearlab.bsky.social
@mdedloff.bsky.social used IFNLR KO and conditional KO mice, flow cytometry of uterus/placenta, and RNAscope of entire gravid uterus to show that maternal DCs mediate the protective effects of IFN-L at the maternal-fetal interface. It was a ton of work, check it out:
www.science.org/doi/10.1126/...
Interferon lambda signaling to maternal dendritic cells protects against congenital Zika virus infection. Dedloff MR, Reinhardt TR, Ropp TF, Rivera-Cardona J, Robles CR, Casazza RL, Ezzell JA, Itano MS, Lazear HM. 2026. Science Advances. Interferon-λ (IFN-λ, type III IFN) mediates antiviral immunity at anatomic barriers, including the maternal-fetal interface. To investigate the effects of IFN-λ during congenital Zika virus (ZIKV) infection, we infected mice lacking the IFN-αβ receptor (Ifnar1−/−) or both the IFN-αβ and IFN-λ receptors (Ifnar1−/−Ifnlr1−/−) at embryonic day 9 and found that loss of maternal IFN-λ signaling resulted in greater transplacental transmission, highlighting a protective effect of IFN-λ at the maternal-fetal interface. To investigate the spatial relationship between ZIKV infection and antiviral responses, we used HiPlex RNAscope on the entire gravid uterus and found that IFN-λ was expressed more proximal to the site of ZIKV infection in Ifnar1−/− dams compared to Ifnar1−/−Ifnlr1−/− dams. We performed immunophenotyping of the placenta and uterus by flow cytometry and found a decrease in dendritic cells and natural killer (NK) cells in the uterus of Ifnar1−/−Ifnlr1−/− dams compared to Ifnar1−/− dams, but NK cell depletion did not affect fetal infection. Using conditional knockout mice, we identified maternal dendritic cells as the key IFN-λ-responsive cell type mediating protection against ZIKV congenital infection. Together, our data support a model in which IFN-λ signaling to maternal dendritic cells increases dendritic cell accumulation in the uterus, which facilitates a protective antiviral response that restricts ZIKV congenital infection. https://www.science.org/doi/10.1126/sciadv.aeh1073
October 27, 2026 at 12:01 PM
cell-regeneration.bsky.social
Cell Regen Research | Interlobar heterogeneity emerges early in the embryonic mouse lung! 🫁
The left lobe shows enriched immune genes and more early gCap endothelial cells; the right cranial lobe differs in gene programs and cell composition.
#StemCells #LungDevelopment
🔗 doi.org/10.1186/s136...
Early inter-lobar heterogeneity of gCap endothelial cells. (A) Schematic of the CCC-Lobe pipeline used to predict cell-type composition across lung lobes. (G–I) Spatial transcriptomics and RNAscope validation show higher Aplnr⁺ (gCap) enrichment in the left lobe than in the right cranial lobe.
October 16, 2026 at 9:06 AM
duckswabber.bsky.social
5 ~23-mo-old Holstein heifers aborted in their last month of gestation - HPAI detected in placenta. 3 placentas had multifocal edema, Matrix protein gene positive in 1 placenta via RNAscope in-situ hybridization
👉 journals.sagepub.com/doi/10.1177/...
October 11, 2026 at 11:54 PM
biologyopen.bsky.social
Lara Feulner and colleagues suggest a role for H19 in maintaining endocardial and valve endothelial cell states during perinatal heart maturation. doi.org/10.1242/bio....
RNAscope staining for selected marker genes across E14.5, P1, and P7 time points.
September 25, 2026 at 11:02 AM
aimmjournal.bsky.social
The Application of RNAscope Protocol to Detect Dengue Virus in Human Paraffin-Embedded Tissue www.ovid.com/10.1097/PAI....
The Application of RNAscope Protocol to Detect... : Applied Immunohistochemistry & Molecular Morphology
Dengue is an important arthropod-borne viral disease, endemic in over 100 countries and challenging health care systems worldwide São Paulo, Brasil....
www.ovid.com
September 18, 2026 at 2:02 AM
bt-spatial.bsky.social
In our upcoming webinar on September 17, Xavier Langa shows how RNAscope™, multiplex imaging, and computational image analysis reveal the biodistribution, target engagement, and safety of ASO and siRNA programs across preclinical tissues.
Register now: https://link.lunaphore.com/3r4
September 4, 2026 at 7:36 AM
bt-spatial.bsky.social
In a new Cell study, Ho et al. combined RNAscope™ RNA detection with multiplex protein imaging and demonstrated how sensory nerve signaling can interfere with the formation of tertiary lymphoid structures in lung adenocarcinoma. Read the full article: https://link.lunaphore.com/um6
August 28, 2026 at 9:10 AM
fens.org
At #FENS2026, Bio-Techne Spatial and R&D Systems are joining forces! From spatial insights powered by COMET™ and RNAscope™ to validated reagents, analytical instruments and expertise by R&D Systems, discover the expanding Bio-Techne portfolio for neuroscience at the FENS Forum.
June 30, 2026 at 9:01 AM
ralfwittenbrink.bsky.social
bekannt sind, in den kritischen Übergang von einem lediglich lästigen COVID-19-Fall zu einem potenziell tödlichen Fall einbezogen. Interstitielle Makrophagen befinden sich tief in der Lunge und schützen dieses kostbare Organ normalerweise unter anderem dadurch, dass sie Viren,
RNAscope multiplex smFISH of infected lung slice culture from Case 2, fixed 72 h after infection.
April 12, 2024 at 9:28 AM
alwardlab-ucla.bsky.social
We’re looking to hire a research tech. Come join our team to sink your teeth into experiments on the hormonal and cellular basis of social behavior. Strong candidates should be proficient in immunohistochemostry/fluorescence, in situ hybridization/HCR/RNAscope, and microscopy. 1/2
May 29, 2025 at 4:31 PM
sabaaltaf.bsky.social
Yes Selene, I did a lot smFISH and RNAscope in brain tissue, happy to help wherever needed.
November 25, 2025 at 5:03 AM
360dx.bsky.social
www.360dx.com/business-new...
Germany's Merck KGaA Inks Deal to Acquire Bio-Teche for $11.3B
Merck KGaA will acquire Bio-Techne's assets including recombinant proteins, antibodies, and immunoassays as well as its ProteinSimple and RNAscope systems.
www.360dx.com
June 25, 2026 at 2:36 PM
asm.org
In #ASMCaseReport: a unique case of HPAI H5N1 virus infection in an immunocompromised domestic cat. This case report cat highlights the need for developing an improved prevention plan for H5N1 infections in pet animals. Read the article: asm.social/2BJ
Photomicrographs of necrotizing lesions in a cat infected by the H5N1 influenza virus. Lesions include necrotizing bronchiolitis and bronchitis (A, D and G), hepatic necrosis (B, E and H), and splenic lymphoid necrosis (C, F and I) with colocalization of H5 RNA or influenza A virus NP antigen. (A–C) Hematoxylin and eosin staining; (D–F) RNAscope in situ hybridization with H5 RNA-specific probe; and (G–I): immunohistochemical staining with anti-NP monoclonal antibody (mAb #42-100).
September 22, 2025 at 4:28 PM
charlottefare.bsky.social
High-throughput quantification of huntingtin mRNA expression and aggregation in mouse brain using automated RNAscope imaging www.biorxiv.org/content/10.6...
www.biorxiv.org
February 16, 2026 at 3:18 PM
oligogirl.bsky.social
The in situ hybridization and RNAscope analyses confirmed the RT-PCR findings. Dystrophin isoform expression was mainly in the neuronal cells, though the Dp427m isoform was expressed in oligodendrocytes (my favorite brain cell!).
June 20, 2025 at 7:44 AM
grnfluoresceblg.bsky.social
"Although fine structures of intercellular protrusions were well preserved in the presence of low concentration of glutaraldehyde as a fixative, the staining condition prevented the detection of mRNAs by RNAscope as discussed previously(59)."

59 is this:
link.springer.com/protocol/10....
Detection of mRNA Transfer Between Mammalian Cells in Coculture by Single-Molecule Fluorescent In Situ Hybridization (smFISH)
In eukaryotic cells, a small percentage of mRNA molecules can undergo transfer from one cell to another. mRNA transfer occurs primarily via membrane nanotubes, which are long thin protrusions that are...
link.springer.com
July 2, 2024 at 8:13 AM
sjaekel.bsky.social
The first paper of the Jäkel lab is out!
We have shown that the RNA detection in human FFPE brain tissue significantly changes with fixation time. Chose your tissue well before doing an experiment!

doi.org/10.1093/brai...
Impact of fixation duration on messenger RNA detectability in human formalin-fixed paraffin embedded brain tissue
Hurler et al. performed RNAScope on post-mortem human brain tissue and found mRNA signal detection to be highly dependent on fixation time. Considering the
doi.org
November 29, 2024 at 5:54 AM
davidweinshenker.bsky.social
Lovely RNAscope image of the rostromedial tegmental nucleus with DAPI (blue), GAD1 (green), galanin 1 receptor (orange), and mu opioid receptor (red). Courtesy of postdoc Brittany Pate.
July 3, 2026 at 4:51 PM
drjennkim.bsky.social
#FluorescenceFriday to mark my first foray into human neuroanatomy @lieberinstitute.bsky.social 🧠 RNAscope of marker genes for the ventral tegmental area (VTA) and nucleus accumbens (Ac) in the developing human brain
March 7, 2026 at 12:10 AM
healthmedicinet.com.web.brid.gy
HMN 2025: How Novel truncated RNAs from leaping DNA encode reverse transcriptases in ageing human mind
Results from utilizing RNAscope expertise to detect novel truncated RNAs within the ageing human mind created by the “leaping” of lengthy interspersed nuclear element-1, a genetic sequence recognized to make use of its personal reverse transcriptases to repeat and paste itself elsewhere within the genome. Credit: Jerold Chun, Juliet Nicodemus, Sanford Burnham Prebys Alzheimer’s illness is the commonest reason behind dementia and impacts greater than a tenth of Americans aged 65 and older. The illness has confirmed tough to develop new remedies for, and accessible remedy choices are restricted. With circumstances within the U.S. projected to greater than double by Read More
healthmedicinet.com
June 7, 2025 at 5:40 AM
ucdgenomecenter.bsky.social
You are invited to an RNAscope Spatial Lunch and Learn event on May 20th. Everybody is welcome to join.
Please see the see the invitation below and use the following link to register:
engage.bio-techne.com/803f1e
Where: GBSF building Auditorium (#1005)
When: Tuesday, May 20th, noon - 2 pm
UC Davis Spatial Biology Lunch and Learn - May 20th
UC Davis Spatial Biology Lunch and Learn - May 20th
engage.bio-techne.com
May 13, 2025 at 4:17 PM