Read the full paper: A room-temperature 89Zr4+ radiolabelling strategy for small extracellular vesicles with enhanced plasma stability for PET imaging

PhD Project - Advancing Isotopic Labelling of Nanoplastics for Environmental Studies at University of Edinburgh, listed on FindAPhD.com

Read the full paper: A room-temperature ⁸⁹Zr⁴⁺ radiolabelling strategy for small extracellular vesicles with enhanced plasma stability for PET Imaging

Animal studies were performed at the Washington University School of Medicine in compliance with institutional guidelines and protocols approved by the Institutional Animal Care and Use Committee (animal protocol 21-0087, 24-0274; IBC protocol 13652, principal investigator: P.M.R.P.). Radiolabelling and PET–CT imaging experiments were performed using appropriately shielded equipment within a licensed radiation-safety facility by trained personnel under an active licence. Preparation of antibody–TCO and antibody–tetrazine conjugates (random and site-specific) Random conjugation Antibodies were conjugated at a molar ratio of 15 TCO-PEG4-NHS-ester (TCO; BroadPharm, BP-22418) or tetrazine-PEG5-NHS-ester (tetrazine: BroadPharm, BP-22681) per antibody. Conjugations were performed in PBS (pH 8.8–9) at 37 °C, 500 rpm for 1 h. Conjugates were purified using a desalting column (PD-10, GE Healthcare) and concentrated using Amicon filters with a 50 kDa molecular weight cutoff (Millipore, UFC8050) in 1× PBS (pH 7.4). For indocyanine green-Sulfo-Osu (ICG) conjugation, trastuzumab–tetrazine was conjugated with ICG (Fisher Scientific, 501952790) at a molar ratio of 3 ICG per antibody in PBS (pH 8.8) at 37 °C, 500 rpm for 1 h. The fluorescently labelled antibody conjugate was purified using a desalting column (PD-10) and concentrated using 50 kDa molecular weight cutoff Amicon filters in 1× PBS (pH 7.4). Site-specific conjugation Antibodies were...

This review explores the landscape of Amaryllidaceae alkaloid biosynthesis, tracing its evolution from radiolabelling studies to multi-omics analyses, emph

Nature Catalysis, Published online: 14 July 2025; doi:10.1038/s41929-025-01366-xEnantioselective catalytic C(sp3)–H fluorination has been limited to electrophilic fluorine sources. Now chiral palladium catalysts bearing amino sulfonamide ligands enable enantioselective incorporation of nucleophilic fluoride into unactivated aliphatic C–H bonds with demonstrated applications to 18F-radiolabelling using [18F]KF.

In this study, we present the first reported use of bioorthogonal click chemistry with rhenium-188 for radiolabelling of an anti-c-Met VHH Nanobody®. We employed a “chelate-then-click” strategy, where...

James Mitchell Crow explains how an unexpected discovery in main group chemistry inspired two decades of chemical creativity, from carbon dioxide reduction to fluorocarbon recycling, offering sustaina...