Glad to share our recent study on the characterization of Nocardia asteroides dihydrofolate reductase (DHFR), now published as a pre-proof in Biochimie hosted by @sfbbm.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Biochemical, structural, and functional characterization of the Nocardia asteroides dihydrofolate reductase: a primary target of anti-nocardiosis treatment
Nocardiosis is a human infectious disease caused by several species of Nocardia and primarily affecting the skin, lungs and central nervous system. Th…
www.sciencedirect.com
October 5, 2026 at 5:03 PM
Glad to share our recent study on the characterization of Nocardia asteroides dihydrofolate reductase (DHFR), now published as a pre-proof in Biochimie hosted by @sfbbm.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Cool new preprint from Mroginski & Zebger et al. on ATP-driven electron transfer in the "archerase" DCCP-R (double cubane cluster protein reductase)
chemrxiv.org/doi/full/10....
chemrxiv.org/doi/full/10....
October 2, 2026 at 10:17 AM
Cool new preprint from Mroginski & Zebger et al. on ATP-driven electron transfer in the "archerase" DCCP-R (double cubane cluster protein reductase)
chemrxiv.org/doi/full/10....
chemrxiv.org/doi/full/10....
Characterization of FAD binding to the apo-form of diflavin-linked disulfide oxidoreductase derived from cyanobacterium Synechocystis sp. PCC6803 portlandpress.com/biochemj/art...
Characterization of FAD binding to the apo-form of diflavin-linked disulfide oxidoreductase derived from cyanobacterium Synechocystis sp. PCC6803
Diflavin-linked disulfide oxidoreductase (DDOR) from the cyanobacterium Synechocystis sp. PCC6803 is an NADPH-thioredoxin reductase (NTR) superfamily enzyme that forms a homodimer containing two FAD molecules (FAD1 and FAD2) per monomer. FAD1 is conserved in the NTR superfamily, while FAD2 is characteristic of DDOR. Under aerobic conditions, thiol reduction of DDOR produces an apparently air-stable neutral blue semiquinone, but the respective roles of FAD1 and FAD2 in the reduction process remain unclear. To address this, spectroscopic, structural and mutational analyses were performed to investigate the contributions of the two FAD molecules. Recombinant apo- and holo-DDOR synthesised in Escherichia coli cells were separated and purified. Fluorescence titration of the apo-DDOR with FAD revealed rapid binding of two FADs. Stopped-flow analysis indicated biphasic binding of FAD to apo-DDOR. X-ray crystal structures of apo- and holo-DDORs exhibited pronounced conformational differences in the FAD-binding sites, suggesting that the first FAD may bind to the FAD1-binding site and subsequently induce formation of the FAD2-binding site. Systematic replacement of amino acid residues in the FAD2-binding site showed that mutations at the characteristic loop region bearing the GKKDSLY motif resulted in binding of three flavin species of FAD, flavin mononucleotide and riboflavin. Moreover, replacement of both Trp53 and Tyr143 at the binding site of the isoalloxazine ring of FAD2 with Ala residues yielded exclusively the apo-form. These results indicate that the loop region restricts the flavin species bound at the FAD2-binding site, and Trp53 and Tyr143 are important for FAD binding to both the FAD1- and FAD2-binding sites.
portlandpress.com
October 2, 2026 at 7:55 AM
Characterization of FAD binding to the apo-form of diflavin-linked disulfide oxidoreductase derived from cyanobacterium Synechocystis sp. PCC6803 portlandpress.com/biochemj/art...
Last chance:
What is the name of Semenya’s intersex condition?
Is it 5a reductase deficiency?
What is the name of Semenya’s intersex condition?
Is it 5a reductase deficiency?
October 1, 2026 at 9:29 PM
Last chance:
What is the name of Semenya’s intersex condition?
Is it 5a reductase deficiency?
What is the name of Semenya’s intersex condition?
Is it 5a reductase deficiency?
Is 5a reductase an intersex condition....Yes or No?
Do words and definitions matter here or don't they?
According to you a woman with a vagina is a male. That contradicts nearly every argument you make about sex and gender.
Do words and definitions matter here or don't they?
According to you a woman with a vagina is a male. That contradicts nearly every argument you make about sex and gender.
October 1, 2026 at 5:45 PM
Is 5a reductase an intersex condition....Yes or No?
Do words and definitions matter here or don't they?
According to you a woman with a vagina is a male. That contradicts nearly every argument you make about sex and gender.
Do words and definitions matter here or don't they?
According to you a woman with a vagina is a male. That contradicts nearly every argument you make about sex and gender.
What does the 2nd sentence say? Answer this and I will enjoy answering your question.
medlineplus.gov/genetics/con...
medlineplus.gov/genetics/con...
5-alpha reductase deficiency: MedlinePlus Genetics
5-alpha reductase deficiency is a condition that affects male sexual development before birth and during puberty. Explore symptoms, inheritance, genetics of this condition.
medlineplus.gov
October 1, 2026 at 5:34 PM
What does the 2nd sentence say? Answer this and I will enjoy answering your question.
medlineplus.gov/genetics/con...
medlineplus.gov/genetics/con...
Where does it say that? Please highlight the sentence for me.
And what does the 2nd sentence say?
medlineplus.gov/genetics/con...
And what does the 2nd sentence say?
medlineplus.gov/genetics/con...
5-alpha reductase deficiency: MedlinePlus Genetics
5-alpha reductase deficiency is a condition that affects male sexual development before birth and during puberty. Explore symptoms, inheritance, genetics of this condition.
medlineplus.gov
October 1, 2026 at 5:33 PM
Where does it say that? Please highlight the sentence for me.
And what does the 2nd sentence say?
medlineplus.gov/genetics/con...
And what does the 2nd sentence say?
medlineplus.gov/genetics/con...
You haven’t answered the question. Not once. Let me try again:
What does the 2nd sentence say and where does it say most people with 5ard are female?
medlineplus.gov/genetics/con...
What does the 2nd sentence say and where does it say most people with 5ard are female?
medlineplus.gov/genetics/con...
5-alpha reductase deficiency: MedlinePlus Genetics
5-alpha reductase deficiency is a condition that affects male sexual development before birth and during puberty. Explore symptoms, inheritance, genetics of this condition.
medlineplus.gov
October 1, 2026 at 5:30 PM
You haven’t answered the question. Not once. Let me try again:
What does the 2nd sentence say and where does it say most people with 5ard are female?
medlineplus.gov/genetics/con...
What does the 2nd sentence say and where does it say most people with 5ard are female?
medlineplus.gov/genetics/con...
Do words mean things or do you get to decide what they mean? Is 5a reductase an intersex condition or not?
There's literally only one correct answer.
Hint: it is!
There's literally only one correct answer.
Hint: it is!
October 1, 2026 at 5:26 PM
Do words mean things or do you get to decide what they mean? Is 5a reductase an intersex condition or not?
There's literally only one correct answer.
Hint: it is!
There's literally only one correct answer.
Hint: it is!
You can’t just ignore the questions I have asked you dozens of times, now, and expect me to answer yours. Be an adult.
What does the 2nd sentence say and where does it say most are women?
medlineplus.gov/genetics/con...
What does the 2nd sentence say and where does it say most are women?
medlineplus.gov/genetics/con...
5-alpha reductase deficiency: MedlinePlus Genetics
5-alpha reductase deficiency is a condition that affects male sexual development before birth and during puberty. Explore symptoms, inheritance, genetics of this condition.
medlineplus.gov
October 1, 2026 at 5:24 PM
You can’t just ignore the questions I have asked you dozens of times, now, and expect me to answer yours. Be an adult.
What does the 2nd sentence say and where does it say most are women?
medlineplus.gov/genetics/con...
What does the 2nd sentence say and where does it say most are women?
medlineplus.gov/genetics/con...
What does the 2nd sentence say and where does it say most people with 5ard are female?
medlineplus.gov/genetics/con...
medlineplus.gov/genetics/con...
5-alpha reductase deficiency: MedlinePlus Genetics
5-alpha reductase deficiency is a condition that affects male sexual development before birth and during puberty. Explore symptoms, inheritance, genetics of this condition.
medlineplus.gov
October 1, 2026 at 5:21 PM
What does the 2nd sentence say and where does it say most people with 5ard are female?
medlineplus.gov/genetics/con...
medlineplus.gov/genetics/con...
What does the 2nd sentence say?
And where does it say most people with 5ard are female?
medlineplus.gov/genetics/con...
And where does it say most people with 5ard are female?
medlineplus.gov/genetics/con...
5-alpha reductase deficiency: MedlinePlus Genetics
5-alpha reductase deficiency is a condition that affects male sexual development before birth and during puberty. Explore symptoms, inheritance, genetics of this condition.
medlineplus.gov
October 1, 2026 at 5:16 PM
What does the 2nd sentence say?
And where does it say most people with 5ard are female?
medlineplus.gov/genetics/con...
And where does it say most people with 5ard are female?
medlineplus.gov/genetics/con...
Highlight where in the study is says what you claim it says.
We both know you cant.😂
What does the 2nd sentence say.
medlineplus.gov/genetics/con...
We both know you cant.😂
What does the 2nd sentence say.
medlineplus.gov/genetics/con...
5-alpha reductase deficiency: MedlinePlus Genetics
5-alpha reductase deficiency is a condition that affects male sexual development before birth and during puberty. Explore symptoms, inheritance, genetics of this condition.
medlineplus.gov
October 1, 2026 at 5:13 PM
Highlight where in the study is says what you claim it says.
We both know you cant.😂
What does the 2nd sentence say.
medlineplus.gov/genetics/con...
We both know you cant.😂
What does the 2nd sentence say.
medlineplus.gov/genetics/con...
ufhealth.org/conditions-a...
People born male don't have 5a reductase. Only people born intersex do. Hence the term.
People born male don't have 5a reductase. Only people born intersex do. Hence the term.
Intersex - UF Health
Differences of sex development (DSD) is a group of conditions in which there is a discrepancy between the external (outside) genitals (penis, scrotum, vulva,…
ufhealth.org
October 1, 2026 at 5:10 PM
ufhealth.org/conditions-a...
People born male don't have 5a reductase. Only people born intersex do. Hence the term.
People born male don't have 5a reductase. Only people born intersex do. Hence the term.
What does the first sentence say?
Why is 5a reductase listed here on a list of types intersex disorders?
ufhealth.org/conditions-a...
Why is 5a reductase listed here on a list of types intersex disorders?
ufhealth.org/conditions-a...
October 1, 2026 at 5:02 PM
What does the first sentence say?
Why is 5a reductase listed here on a list of types intersex disorders?
ufhealth.org/conditions-a...
Why is 5a reductase listed here on a list of types intersex disorders?
ufhealth.org/conditions-a...
Here’s a challenge: give me any article at all that states someone with 5a reductase deficiency xy is not always genetically male and can be female, sometimes.
October 1, 2026 at 5:01 PM
Here’s a challenge: give me any article at all that states someone with 5a reductase deficiency xy is not always genetically male and can be female, sometimes.
That isn’t a medical study on 5a Reductase deficiency. It is irrelevant to the question.
Are people with 5a genetically male?
Are people with 5a genetically male?
October 1, 2026 at 4:57 PM
That isn’t a medical study on 5a Reductase deficiency. It is irrelevant to the question.
Are people with 5a genetically male?
Are people with 5a genetically male?