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Fabrication of #MicrovesselOnAChip
#EndothelialCell HUVEC
3D printing injection reservoirs
Needle-based collagen channel φ200 μm

Sprouting
100 Pa intraluminal pressure
4 & 70 kD FITC-dextran for permeability

A. Bancaud & Y. Matsunaga labs #STARProtoc 2024
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September 3, 2025 at 11:29 AM
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Protocol for a low-volume, direct analysis urine preparation procedure for non-targeted GC-MS metabolomics #STARProtoc www.sciencedirect.com/science/arti...
Protocol for a low-volume, direct analysis urine preparation procedure for non-targeted GC-MS metabolomics
We present a low-volume, direct analysis protocol for non-targeted gas chromatography-mass spectrometry (GC-MS) metabolomics, using 100 μL of urine. T…
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November 17, 2024 at 6:38 PM
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Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomics #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomics
Here, we present a protocol for spatial proteomic profiling of the tumor microenvironment in tonsil cancer using multiplexed imaging-powered deep visu…
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June 21, 2025 at 10:38 AM
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Protocol for label-free quantitative lysine lactylproteome profiling #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol for label-free quantitative lysine lactylproteome profiling
L-lactate has been recognized as an essential molecule for signaling and metabolic balance. Lactylation, a post-translational modification (PTM) deriv…
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April 5, 2025 at 10:50 AM
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Protocol for spatial metabolomics and isotope tracing in the mouse brain #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol for spatial metabolomics and isotope tracing in the mouse brain
Mass spectrometry imaging enables high-resolution spatial chemical mapping, yet its application for dynamic analysis with tracers poses challenges. He…
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March 29, 2025 at 2:18 PM
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Protocol for isolation of lipid droplets and mitochondria from acute myeloid leukemia cells for lipidomic analysis #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol for isolation of lipid droplets and mitochondria from acute myeloid leukemia cells for lipidomic analysis
Lipid droplets (LDs) are dynamic organelles critical for lipid and energy homeostasis. LD dysregulation is implicated in various diseases, including a…
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December 21, 2025 at 7:30 PM
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Protocol for generating protein profiles and distance-based network analysis of murine tissue slices #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol for generating protein profiles and distance-based network analysis of murine tissue slices
We introduce a protocol for spatial proteomics using thin cryotome sections of mouse skeletal muscle tissue. We describe steps for preparing muscle se…
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January 21, 2025 at 12:03 PM
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Mass spectrometry protocol for tear fluid proteome profiling and biomarker discovery #STARProtoc www.sciencedirect.com/science/arti...
Mass spectrometry protocol for tear fluid proteome profiling and biomarker discovery
Here, we present a protocol for tear fluid proteomics using Schirmer strips and liquid chromatography-tandem mass spectrometry (LC-MS/MS). It includes…
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December 17, 2025 at 7:48 PM
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Protocol for structural analysis of amyloid fibrils using hydrogen-deuterium exchange mass spectrometry #STARProtoc www.sciencedirect.com/science/arti...
Protocol for structural analysis of amyloid fibrils using hydrogen-deuterium exchange mass spectrometry
Amyloid fibrils are common in many diseases. Here, we present a protocol that uses α-synuclein fibrils, a Parkinson’s disease hallmark, to demonstrate…
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December 8, 2025 at 8:25 PM
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Protocol to quantify protein phosphorylation in mouse extended pluripotent stem cell-derived blastoids #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol to quantify protein phosphorylation in mouse extended pluripotent stem cell-derived blastoids
Protein phosphorylation is an important post-transcription modification regulating essential cellular activities. Here, we present a proteomics protoc…
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November 6, 2025 at 7:11 PM
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Protocol for the quantification of melatonin and AFMK in cerebrospinal fluid by LC-MS/MS #STARProtoc www.sciencedirect.com/science/arti...
Protocol for the quantification of melatonin and AFMK in cerebrospinal fluid by LC-MS/MS
Melatonin and its oxidant derivative, N-acetyl-N-formyl-5-methoxykynuramine (AFMK), are indolamines present in higher organisms, which are related to …
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October 25, 2025 at 6:22 PM
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Protocol for hybrid mass spectrometry-AI mapping of proteasome-generated HLA class I epitopes #STARProtoc www.sciencedirect.com/science/arti...
Protocol for hybrid mass spectrometry-AI mapping of proteasome-generated HLA class I epitopes
Proteasomes play a central role in antiviral and antitumor cytotoxic immunity, generating most of the >10,000 unique proteome-derived peptides bound t…
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October 24, 2025 at 11:00 AM
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Protocol to pinpoint oxidized and reduced cysteine residues in brown fat using differential alkylation labeling techniques #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol to pinpoint oxidized and reduced cysteine residues in brown fat using differential alkylation labeling techniques
Cysteine post-translational modifications are important for protein functions and protein-protein interactions. Here, we present a protocol to detect …
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October 10, 2025 at 6:02 PM
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Protocol for RNA modification analysis by UHPLC-QqQ MS #STARProtoc www.sciencedirect.com/science/arti...
Protocol for RNA modification analysis by UHPLC-QqQ MS
Over 170 RNA modifications have been reported across domains of life. Here, we present a protocol for identifying and quantifying modified nucleosides…
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September 14, 2025 at 6:33 AM
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Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis
Here, we present a protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis. We describe steps for Lei…
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July 21, 2025 at 2:49 PM
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Protocol for the measurement of secreted acetylcholine in the lumen of mouse intestine using LC-MS #STARProtoc www.sciencedirect.com/science/arti...
Protocol for the measurement of secreted acetylcholine in the lumen of mouse intestine using LC-MS
Acetylcholine (ACh) is a small-molecule neurotransmitter that regulates a wide range of neuronal and non-neuronal physiological functions. The non-neu…
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June 12, 2025 at 10:34 AM
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Protocol to quantitatively assess glycolysis and related carbon metabolic fluxes using stable isotope tracing in Crabtree-positive yeasts #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol to quantitatively assess glycolysis and related carbon metabolic fluxes using stable isotope tracing in Crabtree-positive yeasts
Crabtree-positive yeasts rapidly consume glucose via glycolysis, making it difficult to experimentally estimate their actual glycolytic rate or flux. …
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April 24, 2025 at 1:18 PM
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Protocol for dual metabolomics and proteomics using nanoflow liquid chromatography-tandem mass spectrometry #STARProtoc www.sciencedirect.com/science/arti...
Protocol for dual metabolomics and proteomics using nanoflow liquid chromatography-tandem mass spectrometry
Nanoflow liquid chromatography-tandem mass spectrometry (nLC-MS) benefits untargeted metabolomics by enhancing sensitivity and integrating proteomics …
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April 7, 2025 at 6:32 PM
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Protocol for identification of proteins from deyolked zebrafish embryos #STARProtoc #MassSpec star-protocols.cell.com/protocols/4124
Cell Press: STAR Protocols
STAR Protocols is an open access, peer-reviewed journal from Cell Press. We offer structured, transparent, accessible, and repeatable step-by-step experimental and computational protocols from all are...
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April 5, 2025 at 10:52 AM
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Protocol for isolation, fractionation, and system biology-based profiling of gastrointestinal digested dairy colostrum and milk proteome #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol for isolation, fractionation, and system biology-based profiling of gastrointestinal digested dairy colostrum and milk proteome
Colostrum and milk from dairy sources consist of whey, casein, and fat, which have notable pharmacological properties due to their proteins and peptid…
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March 13, 2025 at 11:34 AM
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Protocol to study secretome interactions using extracellular proximity labeling #STARProtoc #MassSpec www.sciencedirect.com/science/arti...
Protocol to study secretome interactions using extracellular proximity labeling
Biotin ligase-based proximity ligation is a widely used, highly effective technique for the study of in vivo protein-protein interactions. However, th…
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December 15, 2024 at 9:44 AM
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Protocol for feeding strategy and proteomics analysis of zebrafish Danio rerio using S-trap and iTRAQ techniques #STARProtoc www.sciencedirect.com/science/arti...
Protocol for feeding strategy and proteomics analysis of zebrafish Danio rerio using S-trap and iTRAQ techniques
Yeast serves as a functional alternative and sustainable protein source in aquaculture. This protocol outlines feeding strategy and intestinal proteom…
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December 12, 2024 at 1:06 PM