Happy to share this highlight of our latest work on the minor spliceosome structure
The Galej Group provides new insights into the minor spliceosome, a vital RNA-protein complex involved in gene expression.
These results allow a better understanding of its mechanism, whose dysfunction is associated with several genetic disorders.
#MolBiol 🧪🧬🧶
www.embl.org/news/science...
These results allow a better understanding of its mechanism, whose dysfunction is associated with several genetic disorders.
#MolBiol 🧪🧬🧶
www.embl.org/news/science...
February 12, 2025 at 3:32 PM
Happy to share this highlight of our latest work on the minor spliceosome structure
Interesting.
Full deletion of all introns in yeast was actually my project in the Staley lab, *20 years ago*. I actually found a bunch of phenotypes. We did this in collaboration with the Guthrie lab.
www.cell.com/cell/abstrac...
Full deletion of all introns in yeast was actually my project in the Staley lab, *20 years ago*. I actually found a bunch of phenotypes. We did this in collaboration with the Guthrie lab.
www.cell.com/cell/abstrac...
A spliceosome-independent eukaryote generated by complete intron removal
Elimination of all spliceosomal introns reveals a spliceosome-independent eukaryote.
www.cell.com
July 15, 2026 at 4:28 PM
Interesting.
Full deletion of all introns in yeast was actually my project in the Staley lab, *20 years ago*. I actually found a bunch of phenotypes. We did this in collaboration with the Guthrie lab.
www.cell.com/cell/abstrac...
Full deletion of all introns in yeast was actually my project in the Staley lab, *20 years ago*. I actually found a bunch of phenotypes. We did this in collaboration with the Guthrie lab.
www.cell.com/cell/abstrac...
country roads 🎵
spliceosome 🎵
removing all, my introns 🎵
spliceosome 🎵
removing all, my introns 🎵
April 14, 2024 at 3:39 PM
country roads 🎵
spliceosome 🎵
removing all, my introns 🎵
spliceosome 🎵
removing all, my introns 🎵
mRNA after leaving the spliceosome:
not even released and she's already got the variants.....
Taylor Swift will release her new ‘Toy Story 5’ single “I Knew It, I Knew You” this Friday.
June 1, 2026 at 8:49 PM
mRNA after leaving the spliceosome:
Researchers at the Centre for Genomic Regulation (CRG) in Barcelona have created the first blueprint of the human spliceosome, the most complex and intricate molecular machine inside every cell. The scientific feat, which took more than a decade to complete, is published in the journal Science.
November 11, 2024 at 5:07 PM
Researchers at the Centre for Genomic Regulation (CRG) in Barcelona have created the first blueprint of the human spliceosome, the most complex and intricate molecular machine inside every cell. The scientific feat, which took more than a decade to complete, is published in the journal Science.
🧬 New from Johnson et al!
📄Bi-allelic variants in the non-protein-coding minor spliceosome components RNU6ATAC and RNU4ATAC cause syndromic monogenic autoimmune diabetes
📄Bi-allelic variants in the non-protein-coding minor spliceosome components RNU6ATAC and RNU4ATAC cause syndromic monogenic autoimmune diabetes
Bi-allelic variants in the non-protein-coding minor spliceosome components RNU6ATAC and RNU4ATAC cause syndromic monogenic autoimmune diabetes
We show that bi-allelic variants in the minor spliceosome snRNAs RNU6ATAC and RNU4ATAC
cause early-onset autoimmune diabetes with immune dysregulation. We define a shared
mechanism involving U12 intro...
www.cell.com
March 20, 2026 at 3:39 PM
🧬 New from Johnson et al!
📄Bi-allelic variants in the non-protein-coding minor spliceosome components RNU6ATAC and RNU4ATAC cause syndromic monogenic autoimmune diabetes
📄Bi-allelic variants in the non-protein-coding minor spliceosome components RNU6ATAC and RNU4ATAC cause syndromic monogenic autoimmune diabetes
Hot and splicy 🔥 Check out spliceosome complexes under quality control by GPATCH1-DHX35! We establish C. Thermophilum as serious player when it comes to spliceosomes. Great collaboration between the Hurt, Jingdong Cheng, Fei Xavier Chen and Lührmann labs. rdcu.be/ebBAx #spliceosome #somelikeithot
Structural insights into spliceosome fidelity: DHX35–GPATCH1- mediated rejection of aberrant splicing substrates
Cell Research - Structural insights into spliceosome fidelity: DHX35–GPATCH1- mediated rejection of aberrant splicing substrates
rdcu.be
February 28, 2025 at 6:09 AM
Hot and splicy 🔥 Check out spliceosome complexes under quality control by GPATCH1-DHX35! We establish C. Thermophilum as serious player when it comes to spliceosomes. Great collaboration between the Hurt, Jingdong Cheng, Fei Xavier Chen and Lührmann labs. rdcu.be/ebBAx #spliceosome #somelikeithot
This would seem to throw some cold water on the @cellpress.bsky.social paper claiming to have made "the first eukaryote functionally independent of the spliceosome"
July 15, 2026 at 6:29 PM
This would seem to throw some cold water on the @cellpress.bsky.social paper claiming to have made "the first eukaryote functionally independent of the spliceosome"
Great talk by @delphinem-p.bsky.social from @geminiteamlab.bsky.social yesterday: “Hijacking the Plant #Spliceosome” on how alternative splicing drives #viral infection and #tomato fruit development 🍅
Thanks to our host @incavirus.bsky.social for organizing this insightful seminar! 👏
Thanks to our host @incavirus.bsky.social for organizing this insightful seminar! 👏
November 27, 2025 at 9:42 AM
Great talk by @delphinem-p.bsky.social from @geminiteamlab.bsky.social yesterday: “Hijacking the Plant #Spliceosome” on how alternative splicing drives #viral infection and #tomato fruit development 🍅
Thanks to our host @incavirus.bsky.social for organizing this insightful seminar! 👏
Thanks to our host @incavirus.bsky.social for organizing this insightful seminar! 👏
Happy to share our latest work on the previously uncharacterized splicing factor Cwc15! Importantly, all of the people who did this work are/were undergrads!
S. cerevisiae Cwc15p Tunes the Spliceosome Active Site for 5' Splice Site Cleavage
www.biorxiv.org/content/10.6...
S. cerevisiae Cwc15p Tunes the Spliceosome Active Site for 5' Splice Site Cleavage
www.biorxiv.org/content/10.6...
www.biorxiv.org
March 23, 2026 at 7:43 PM
Happy to share our latest work on the previously uncharacterized splicing factor Cwc15! Importantly, all of the people who did this work are/were undergrads!
S. cerevisiae Cwc15p Tunes the Spliceosome Active Site for 5' Splice Site Cleavage
www.biorxiv.org/content/10.6...
S. cerevisiae Cwc15p Tunes the Spliceosome Active Site for 5' Splice Site Cleavage
www.biorxiv.org/content/10.6...
The figures in this paper are spectacular. I especially like Sup. Fig. 8.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structural basis of step II spliced leader RNA trans-splicing in trypanosomatid parasites - Nature Communications
Trypanosomatid parasites rely on Spliced Leader (SL) RNA trans-splicing to produce every mature mRNA. Here, the authors present cryo-EM structures of the trans spliceosome during SL exon ligation, pro...
www.nature.com
September 24, 2026 at 12:52 PM
The figures in this paper are spectacular. I especially like Sup. Fig. 8.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The Galej Group provides new insights into the minor spliceosome, a vital RNA-protein complex involved in gene expression.
These results allow a better understanding of its mechanism, whose dysfunction is associated with several genetic disorders.
#MolBiol 🧪🧬🧶
www.embl.org/news/science...
These results allow a better understanding of its mechanism, whose dysfunction is associated with several genetic disorders.
#MolBiol 🧪🧬🧶
www.embl.org/news/science...
February 12, 2025 at 8:49 AM
The Galej Group provides new insights into the minor spliceosome, a vital RNA-protein complex involved in gene expression.
These results allow a better understanding of its mechanism, whose dysfunction is associated with several genetic disorders.
#MolBiol 🧪🧬🧶
www.embl.org/news/science...
These results allow a better understanding of its mechanism, whose dysfunction is associated with several genetic disorders.
#MolBiol 🧪🧬🧶
www.embl.org/news/science...
Knockdown of the fly spliceosome component Rbp1 (orthologue of SRSF1) extends lifespan
#Drosophila
#Drosophila
Knockdown of the fly spliceosome component Rbp1 (orthologue of SRSF1) extends lifespan
#Drosophila
PubMed link
pubmed.ncbi.nlm.nih.gov
January 10, 2026 at 6:23 AM
Knockdown of the fly spliceosome component Rbp1 (orthologue of SRSF1) extends lifespan
#Drosophila
#Drosophila
In our recent review, we explore how the spliceosome is targeted by effectors from diverse plant-interacting organisms—and how these effectors can serve as powerful molecular probes to uncover the mechanisms of splicing regulation in plants.
@geminiteamlab.bsky.social
#PlantScience #RNA #Splicing
@geminiteamlab.bsky.social
#PlantScience #RNA #Splicing
New insights into microbial manipulation of the plant spliceosome
Abstract. Alternative splicing (AS) has emerged as a regulatory layer in plant adaptation to the environment. In particular, biotic stresses trigger a dras
academic.oup.com
March 18, 2026 at 9:05 PM
In our recent review, we explore how the spliceosome is targeted by effectors from diverse plant-interacting organisms—and how these effectors can serve as powerful molecular probes to uncover the mechanisms of splicing regulation in plants.
@geminiteamlab.bsky.social
#PlantScience #RNA #Splicing
@geminiteamlab.bsky.social
#PlantScience #RNA #Splicing
ZBP1 senses spliceosome stress through Z-RNA:DNA hybrid recognition
ZBP1 senses spliceosome stress through Z-RNA:DNA hybrid recognition
He et al. developed Zα-mFc, a probe designed to specifically detect Z-form nucleic acids in cells. Using this probe, they demonstrated that spliceosome inhibition leads to nuclear accumulation of Z-RNA:DNA hybrids. These hybrids are sensed by ZBP1 via its Zα domains, subsequently triggering apoptosis and necroptosis.
dlvr.it
May 2, 2025 at 10:57 PM
ZBP1 senses spliceosome stress through Z-RNA:DNA hybrid recognition
What a way to start 2025! Our spliceosome blueprint has been recognized by @forbes.com as one of the “Top Five Breakthroughs of 2024”. Huge thanks to my colleagues and my mentor Juan Valcarcel at @crg.eu #Biotech #Spliceosome #RNA #Innovation
www.forbes.com/sites/alexkn...
www.forbes.com/sites/alexkn...
Five Breakthroughs In 2024 That Laid A Foundation For Future Tech
From transforming coal to computing with DNA to pharmaceutical manufacturing in space, these are the discoveries that will set the stage for the next decade’s technology.
www.forbes.com
January 2, 2025 at 9:42 AM
What a way to start 2025! Our spliceosome blueprint has been recognized by @forbes.com as one of the “Top Five Breakthroughs of 2024”. Huge thanks to my colleagues and my mentor Juan Valcarcel at @crg.eu #Biotech #Spliceosome #RNA #Innovation
www.forbes.com/sites/alexkn...
www.forbes.com/sites/alexkn...
Cool story - SOS splicing to remove transposons in mRNA! Though not by the spliceosome.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
An RNA Splicing System that Excises Transposons from Animal mRNAs
All genomes harbor mobile genetic parasites called transposable elements (TEs). Here we describe a system, which we term SOS splicing, that protects C. elegans and human genes from DNA transposon-medi...
www.biorxiv.org
June 28, 2025 at 9:11 AM
Cool story - SOS splicing to remove transposons in mRNA! Though not by the spliceosome.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Have a look at our latest results on #CDK regulation, just posted as a pre-print! This study was spearheaded by PhD student Amy McGeoch. Amy has determined the #cryoEM structure of the CDK11-cyclin L-SAP30B complex, an important regulator of #spliceosome activation.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
March 27, 2026 at 7:42 AM
Have a look at our latest results on #CDK regulation, just posted as a pre-print! This study was spearheaded by PhD student Amy McGeoch. Amy has determined the #cryoEM structure of the CDK11-cyclin L-SAP30B complex, an important regulator of #spliceosome activation.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
please go check out this glowing pink blob and what it does to the shiny gold blob
(it's a spliceosome! Truly it was a nostalgic pleasure to help out with this paper, a super fun collaboration with @uwmadisonrna.bsky.social & Kathy and Karli)
(it's a spliceosome! Truly it was a nostalgic pleasure to help out with this paper, a super fun collaboration with @uwmadisonrna.bsky.social & Kathy and Karli)
A splicing factor called Fyv6 controls the selection of 3ʹ splice sites in yeast.
https://buff.ly/49aQvWQ
https://buff.ly/49aQvWQ
December 2, 2024 at 5:05 PM
please go check out this glowing pink blob and what it does to the shiny gold blob
(it's a spliceosome! Truly it was a nostalgic pleasure to help out with this paper, a super fun collaboration with @uwmadisonrna.bsky.social & Kathy and Karli)
(it's a spliceosome! Truly it was a nostalgic pleasure to help out with this paper, a super fun collaboration with @uwmadisonrna.bsky.social & Kathy and Karli)
Nice review of two recent (and it turns out highly similar) structures of stalled spliceosomes, likely on their way to disassembly. These are really complex papers (even for someone really into splicing) and @wojtekgalej.bsky.social 's review is a great help!
rdcu.be/ej8U9
rdcu.be/ej8U9
Spliceosome-associated quality control
Cell Research - Spliceosome-associated quality control
rdcu.be
April 30, 2025 at 8:41 PM
Nice review of two recent (and it turns out highly similar) structures of stalled spliceosomes, likely on their way to disassembly. These are really complex papers (even for someone really into splicing) and @wojtekgalej.bsky.social 's review is a great help!
rdcu.be/ej8U9
rdcu.be/ej8U9
✂️ The spliceosome has to shed Dib1 before it can start cutting RNA. New #Yeast research shows Dib1's C-terminal tail is what keeps it docked near the catalytic core via Prp8 and Prp31, and losing that tail is enough to kill the cell.
🧬 #YeastResearch 🧪 #SGD 🔬
www.yeastgenome.org/reference/S1...
🧬 #YeastResearch 🧪 #SGD 🔬
www.yeastgenome.org/reference/S1...
September 24, 2026 at 12:01 AM
✂️ The spliceosome has to shed Dib1 before it can start cutting RNA. New #Yeast research shows Dib1's C-terminal tail is what keeps it docked near the catalytic core via Prp8 and Prp31, and losing that tail is enough to kill the cell.
🧬 #YeastResearch 🧪 #SGD 🔬
www.yeastgenome.org/reference/S1...
🧬 #YeastResearch 🧪 #SGD 🔬
www.yeastgenome.org/reference/S1...
My graduate work from the Singh lab is now out on bioRxiv. In it we look at the impacts on nonsense mediated mRNA decay when the spliceosome is disrupted in different ways.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Direct and indirect effects of spliceosome disruption compromise gene regulation by Nonsense-Mediated mRNA Decay
Pre-mRNA splicing, carried out in the nucleus by a large ribonucleoprotein machine known as the spliceosome, is functionally and physically coupled to the mRNA surveillance pathway in the cytoplasm ca...
www.biorxiv.org
December 29, 2024 at 7:13 PM
My graduate work from the Singh lab is now out on bioRxiv. In it we look at the impacts on nonsense mediated mRNA decay when the spliceosome is disrupted in different ways.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...