Are you using 5xFAD mice to study Alzheimer’s disease? Our work out today @cp-neuron.bsky.social may be relevant to you 🐁🧠 Age and sex are known to influence Aβ plaque burden in these mice. Here, we found that the transgene parentage is another strong determinant.
Thread below: (1/11)
Thread below: (1/11)
Parental origin of transgene modulates amyloid-β plaque burden in the 5xFAD mouse model of Alzheimer’s disease
The 5xFAD transgenic model, popular in Alzheimer’s disease research, exhibits varying
cerebral plaque burden depending on the parental source of the transgene. Mice inheriting
the transgene paternally...
www.cell.com
January 20, 2025 at 3:49 PM
Are you using 5xFAD mice to study Alzheimer’s disease? Our work out today @cp-neuron.bsky.social may be relevant to you 🐁🧠 Age and sex are known to influence Aβ plaque burden in these mice. Here, we found that the transgene parentage is another strong determinant.
Thread below: (1/11)
Thread below: (1/11)
Losing sleep over #iPSC transgene silencing after differentiation?
Same here — too many times😭
So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.
Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...
Supported by @cziscience.bsky.social
Same here — too many times😭
So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.
Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...
Supported by @cziscience.bsky.social
Prevention of Transgene Silencing During Human Pluripotent Stem Cell Differentiation
While high and stable transgene expression can be achieved in undifferentiated pluripotent stem cells, conventional transgene expression systems are often silenced upon differentiation. Silencing occu...
www.biorxiv.org
April 15, 2025 at 3:37 PM
Losing sleep over #iPSC transgene silencing after differentiation?
Same here — too many times😭
So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.
Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...
Supported by @cziscience.bsky.social
Same here — too many times😭
So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.
Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...
Supported by @cziscience.bsky.social
Programmable promoter editing for precise control of transgene expression - @mitcheme.bsky.social @mitdeptofbe.bsky.social go.nature.com/4h9wlR1
October 13, 2025 at 7:11 PM
Programmable promoter editing for precise control of transgene expression - @mitcheme.bsky.social @mitdeptofbe.bsky.social go.nature.com/4h9wlR1
For #fluorescencefriday 👀 #lightsheet 🔬 M83 mice expressing human alpha-synuclein transgene injected with preformed fibrils of Syn in the striatum. Syn S129 pathology (yellow) propagates along neuronal pathways and follows transgene expression by Vlad Novikov and @shahrzadbahrampour.bsky.social
April 18, 2025 at 10:37 AM
For #fluorescencefriday 👀 #lightsheet 🔬 M83 mice expressing human alpha-synuclein transgene injected with preformed fibrils of Syn in the striatum. Syn S129 pathology (yellow) propagates along neuronal pathways and follows transgene expression by Vlad Novikov and @shahrzadbahrampour.bsky.social
Parents matter - important study for modeling amyloid accumulation 🐭
Parental origin of transgene modulates amyloid-β plaque burden in the 5xFAD mouse model of Alzheimer’s disease
The 5xFAD transgenic model, popular in Alzheimer’s disease research, exhibits varying
cerebral plaque burden depending on the parental source of the transgene. Mice inheriting
the transgene paternally...
www.cell.com
January 20, 2025 at 8:50 PM
Parents matter - important study for modeling amyloid accumulation 🐭
Our paper is just out www.nature.com/articles/s41... from the Jacobsen, Doudna, and Banfield labs on viral delivery of a tiny CRISPR system for transgene free germline editing in plants, tinyurl.com/3k223r3x. Looking for postdocs to join the team for the next phase of this work!
Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis - Nature Plants
Weiss et al. engineered the tobacco rattle virus to express the RNA-guided TnpB enzyme ISYmu1 and guide RNA for transgene-free germline editing in Arabidopsis thaliana.
www.nature.com
May 1, 2025 at 2:24 PM
Our paper is just out www.nature.com/articles/s41... from the Jacobsen, Doudna, and Banfield labs on viral delivery of a tiny CRISPR system for transgene free germline editing in plants, tinyurl.com/3k223r3x. Looking for postdocs to join the team for the next phase of this work!
Trump streicht 8 Millionen Dollar für Krebsforschung, weil er transgender Mäuse versteht, wenn es um transgene Mäuse geht.
Völlig normal für einen, der nun das Bildungsministerium streichen wird. Soll ja keiner klüger sein als der König.
Oh Herr, es ist alles nur eine göttliche Prüfung…
Völlig normal für einen, der nun das Bildungsministerium streichen wird. Soll ja keiner klüger sein als der König.
Oh Herr, es ist alles nur eine göttliche Prüfung…
March 6, 2025 at 7:13 PM
Trump streicht 8 Millionen Dollar für Krebsforschung, weil er transgender Mäuse versteht, wenn es um transgene Mäuse geht.
Völlig normal für einen, der nun das Bildungsministerium streichen wird. Soll ja keiner klüger sein als der König.
Oh Herr, es ist alles nur eine göttliche Prüfung…
Völlig normal für einen, der nun das Bildungsministerium streichen wird. Soll ja keiner klüger sein als der König.
Oh Herr, es ist alles nur eine göttliche Prüfung…
Please RT: Published today, a super fun collaboration w/ @fanjumeng.bsky.social and Marnie Halpern's lab to establish methods for transgene mapping in zebrafish - TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation: Cell Reports Methods www.cell.com/cell-reports...
TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation
Meng et al. develop a method called TransTag for simple and efficient mapping of Tol2-based
transgenes in zebrafish. TransTag robustly identifies transgene insertion sites and
offers an alignment-free...
www.cell.com
July 8, 2025 at 5:07 PM
Please RT: Published today, a super fun collaboration w/ @fanjumeng.bsky.social and Marnie Halpern's lab to establish methods for transgene mapping in zebrafish - TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation: Cell Reports Methods www.cell.com/cell-reports...
Sie sollen in Zukunft nicht wie Transgene Pflanzen reguliert werden, sondern wie klassisch gezüchtete Pflanzen. Das gleiche gilt für Pflanzen, in denen durch Bestrahlung oder Chemikalien zig Tausende, völlig willkürliche Mutationen ausgelöst werden, schon seit Jahrzehnten.
7/
7/
March 29, 2026 at 1:24 PM
Sie sollen in Zukunft nicht wie Transgene Pflanzen reguliert werden, sondern wie klassisch gezüchtete Pflanzen. Das gleiche gilt für Pflanzen, in denen durch Bestrahlung oder Chemikalien zig Tausende, völlig willkürliche Mutationen ausgelöst werden, schon seit Jahrzehnten.
7/
7/
Amazing team science effort, led by Marius Wernig’s group, to develop a silencing-resistant transgene cassette that maintains expression following iPSC differentiation. This was major barrier for iPSC-based experiments. Thanks to CZI for funding the research!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Prevention of Transgene Silencing During Human Pluripotent Stem Cell Differentiation
While high and stable transgene expression can be achieved in undifferentiated pluripotent stem cells, conventional transgene expression systems are often silenced upon differentiation. Silencing occu...
www.biorxiv.org
April 16, 2025 at 6:13 PM
Amazing team science effort, led by Marius Wernig’s group, to develop a silencing-resistant transgene cassette that maintains expression following iPSC differentiation. This was major barrier for iPSC-based experiments. Thanks to CZI for funding the research!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
New OA Letter: "Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis" rdcu.be/eiJVL
...engineered the tobacco rattle virus to express the RNA-guided TnpB enzyme ISYmu1 and guide RNA for transgene-free germline editing.
...engineered the tobacco rattle virus to express the RNA-guided TnpB enzyme ISYmu1 and guide RNA for transgene-free germline editing.
April 22, 2025 at 11:29 AM
New OA Letter: "Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis" rdcu.be/eiJVL
...engineered the tobacco rattle virus to express the RNA-guided TnpB enzyme ISYmu1 and guide RNA for transgene-free germline editing.
...engineered the tobacco rattle virus to express the RNA-guided TnpB enzyme ISYmu1 and guide RNA for transgene-free germline editing.
SARS-CoV-2 verursacht Schäden im Magen
Dies ist die erste Studie, die die Auswirkungen von SARS-CoV-2 auf verschiedene Zelltypen im Magengewebe sowie die Beteiligung von Schutzmechanismen und Zelltod-Pfaden untersucht.
„K18-hACE2-transgene Mäuse (n = 20) wurden in Kontroll- …
Dies ist die erste Studie, die die Auswirkungen von SARS-CoV-2 auf verschiedene Zelltypen im Magengewebe sowie die Beteiligung von Schutzmechanismen und Zelltod-Pfaden untersucht.
„K18-hACE2-transgene Mäuse (n = 20) wurden in Kontroll- …
June 23, 2025 at 7:23 PM
SARS-CoV-2 verursacht Schäden im Magen
Dies ist die erste Studie, die die Auswirkungen von SARS-CoV-2 auf verschiedene Zelltypen im Magengewebe sowie die Beteiligung von Schutzmechanismen und Zelltod-Pfaden untersucht.
„K18-hACE2-transgene Mäuse (n = 20) wurden in Kontroll- …
Dies ist die erste Studie, die die Auswirkungen von SARS-CoV-2 auf verschiedene Zelltypen im Magengewebe sowie die Beteiligung von Schutzmechanismen und Zelltod-Pfaden untersucht.
„K18-hACE2-transgene Mäuse (n = 20) wurden in Kontroll- …
Big news from our lab: we can now stably edit the genome of a parasitic plant! Transgene-free, genome-edited P. japonicum in one generation! This will transform how we study #parasiticplants and other hard-to-transform plants.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
February 12, 2026 at 9:24 AM
Big news from our lab: we can now stably edit the genome of a parasitic plant! Transgene-free, genome-edited P. japonicum in one generation! This will transform how we study #parasiticplants and other hard-to-transform plants.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Die bestehende Regulierung für Gentechnik (im Kern in der sog. Freisetzungsrichtlinie festgeschrieben) wird gar nicht angefasst. Dort geht es vor allem darum, Organismen nach einem sog. Gentransfer zu regulieren. Also zum Beispiel Pflanzen mit Genen aus Bakterien, sog. Transgene Pflanzen.
4/
4/
March 29, 2026 at 1:24 PM
Die bestehende Regulierung für Gentechnik (im Kern in der sog. Freisetzungsrichtlinie festgeschrieben) wird gar nicht angefasst. Dort geht es vor allem darum, Organismen nach einem sog. Gentransfer zu regulieren. Also zum Beispiel Pflanzen mit Genen aus Bakterien, sog. Transgene Pflanzen.
4/
4/
Jo. Und dann gibt es die nicht-kennzeichnungspflichtige, immer transgene Nutzpflanze, die gerade im gesundheitsbewussten Milieu recht beliebt ist 😉
June 17, 2026 at 3:05 PM
Jo. Und dann gibt es die nicht-kennzeichnungspflichtige, immer transgene Nutzpflanze, die gerade im gesundheitsbewussten Milieu recht beliebt ist 😉
Amazing new work from the lab of @aliceyting.bsky.social @stanfordmedicine.bsky.social @stanfordbiosci.bsky.social, engineered GPCRs for ligand-activated cell responses
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Synthetic GPCRs for programmable sensing and control of cell behaviour - Nature
Modular synthetic G-protein-coupled receptors with nanobody-based ligand-recognition domains can be designed and used to programme transgene expression, real-time fluorescence or endogenous G-protein ...
www.nature.com
December 5, 2024 at 2:02 PM
Amazing new work from the lab of @aliceyting.bsky.social @stanfordmedicine.bsky.social @stanfordbiosci.bsky.social, engineered GPCRs for ligand-activated cell responses
www.nature.com/articles/s41...
www.nature.com/articles/s41...
DIAL designs synthetic promoters for generation of heritable setpoints of gene expression across a range of cell types go.nature.com/4h9wlR1
rdcu.be/ruJgZuhLT6ra
rdcu.be/ruJgZuhLT6ra
Programmable promoter editing for precise control of transgene expression - Nature Biotechnology
DIAL designs synthetic promoters for generation of heritable setpoints of gene expression across a range of cell types.
go.nature.com
September 29, 2026 at 3:32 AM
DIAL designs synthetic promoters for generation of heritable setpoints of gene expression across a range of cell types go.nature.com/4h9wlR1
rdcu.be/ruJgZuhLT6ra
rdcu.be/ruJgZuhLT6ra
Galaxy of microtubules in the embryonic zebrafish eye. Credit to @zebrafish007.bsky.social. #ZebrafishZunday 🧪
June 15, 2025 at 3:15 PM
Galaxy of microtubules in the embryonic zebrafish eye. Credit to @zebrafish007.bsky.social. #ZebrafishZunday 🧪
Genome editing in brown algae! 🧬🪸🌿 Now out in Cell Reports Methods!
Excited to share this highly efficient, transgene-free CRISPR–Cas genome editing protocol for brown algae, requiring no cloning and no specialized equipment.
doi.org/10.1016/j.cr...
#CRISPR #BrownAlgae
@mpi-bio-fml.bsky.social
Excited to share this highly efficient, transgene-free CRISPR–Cas genome editing protocol for brown algae, requiring no cloning and no specialized equipment.
doi.org/10.1016/j.cr...
#CRISPR #BrownAlgae
@mpi-bio-fml.bsky.social
January 2, 2026 at 2:20 PM
Genome editing in brown algae! 🧬🪸🌿 Now out in Cell Reports Methods!
Excited to share this highly efficient, transgene-free CRISPR–Cas genome editing protocol for brown algae, requiring no cloning and no specialized equipment.
doi.org/10.1016/j.cr...
#CRISPR #BrownAlgae
@mpi-bio-fml.bsky.social
Excited to share this highly efficient, transgene-free CRISPR–Cas genome editing protocol for brown algae, requiring no cloning and no specialized equipment.
doi.org/10.1016/j.cr...
#CRISPR #BrownAlgae
@mpi-bio-fml.bsky.social
1/n Fun journey continues: Our new @cp-cellstemcell.bsky.social paper out on how the Inherent & Dormant Plasticity of Mouse Naive PSCs can be "Exaggerated" to Directly form ~E8.5 Transgene-Free (TF) Mouse SEMs & WITHOUT inducing 8-16-Morula-like "Embryo Founder Cells".
www.cell.com/cell-stem-ce...
www.cell.com/cell-stem-ce...
Transgene-free generation of mouse post-gastrulation whole embryo models solely from naive ESCs and iPSCs
Hanna and colleagues show that signaling pathway modulation amplifies the inherent
flexibility of genetically unmodified mouse naive PSCs to adopt extra-embryonic cell
fate potential. This endows them...
www.cell.com
August 7, 2025 at 3:29 PM
1/n Fun journey continues: Our new @cp-cellstemcell.bsky.social paper out on how the Inherent & Dormant Plasticity of Mouse Naive PSCs can be "Exaggerated" to Directly form ~E8.5 Transgene-Free (TF) Mouse SEMs & WITHOUT inducing 8-16-Morula-like "Embryo Founder Cells".
www.cell.com/cell-stem-ce...
www.cell.com/cell-stem-ce...
🌱 From The Plant Cell: Identifying an aberrant RNA trigger reveals why some engineered genes shut down, guiding design of more stable traits in crops. (Richard Michelmore , Blake C Meyers)
▶️ academic.oup.com/plcell/artic...
#PlantScience #PlantBiology
▶️ academic.oup.com/plcell/artic...
#PlantScience #PlantBiology
Identification of a cleaved aberrant RNA associated with the initiation of transgene silencing
Target capture enriched long-read RNA-sequencing uncovers a cleaved aberrant RNA from a ribosome stalling event that is associated with transgene silencing
academic.oup.com
April 29, 2026 at 8:37 PM
🌱 From The Plant Cell: Identifying an aberrant RNA trigger reveals why some engineered genes shut down, guiding design of more stable traits in crops. (Richard Michelmore , Blake C Meyers)
▶️ academic.oup.com/plcell/artic...
#PlantScience #PlantBiology
▶️ academic.oup.com/plcell/artic...
#PlantScience #PlantBiology
5′ UTR introns reduce nuclear transgene silencing and enable robust protein expression in Chlamydomonas reinhardtii
www.cell.com/cell-reports...
www.cell.com/cell-reports...
5′ UTR introns reduce nuclear transgene silencing and enable robust protein expression in Chlamydomonas reinhardtii
Nuclear transgene expression in Chlamydomonas is frequently limited by gene silencing.
Xing et al. show that intron-containing 5′ UTR regulatory elements can reduce nuclear
gene silencing via epigenet...
www.cell.com
May 24, 2026 at 1:27 PM
5′ UTR introns reduce nuclear transgene silencing and enable robust protein expression in Chlamydomonas reinhardtii
www.cell.com/cell-reports...
www.cell.com/cell-reports...