October 11, 2023 at 7:53 AM
Clinical Pseudomonas aeruginosa can carry a fourth T6SS, but is it functional?
#mLife from @taogd.bsky.social lab: H4-T6SS becomes an active antibacterial machine when switched on, and TseMt is a pore-forming toxin with a 3.0 Å structure and a dedicated delivery route. #T6SS
doi.org/10.1002/mlf2...
#mLife from @taogd.bsky.social lab: H4-T6SS becomes an active antibacterial machine when switched on, and TseMt is a pore-forming toxin with a 3.0 Å structure and a dedicated delivery route. #T6SS
doi.org/10.1002/mlf2...
September 4, 2026 at 1:55 AM
Clinical Pseudomonas aeruginosa can carry a fourth T6SS, but is it functional?
#mLife from @taogd.bsky.social lab: H4-T6SS becomes an active antibacterial machine when switched on, and TseMt is a pore-forming toxin with a 3.0 Å structure and a dedicated delivery route. #T6SS
doi.org/10.1002/mlf2...
#mLife from @taogd.bsky.social lab: H4-T6SS becomes an active antibacterial machine when switched on, and TseMt is a pore-forming toxin with a 3.0 Å structure and a dedicated delivery route. #T6SS
doi.org/10.1002/mlf2...
Published in mLife, this study reveals that Pseudomonas aeruginosa is a major nosocomial pathogen whose type VI secretion systems (T6SSs) mediate bacterial competition and host-cell interactions.
#T6SS #TseMt
Details: doi.org/10.1002/mlf2...
#T6SS #TseMt
Details: doi.org/10.1002/mlf2...
September 4, 2026 at 3:50 PM
Published in mLife, this study reveals that Pseudomonas aeruginosa is a major nosocomial pathogen whose type VI secretion systems (T6SSs) mediate bacterial competition and host-cell interactions.
#T6SS #TseMt
Details: doi.org/10.1002/mlf2...
#T6SS #TseMt
Details: doi.org/10.1002/mlf2...
Genetics and structural modeling indicate a dedicated DUF4123 chaperone-VgrG4b-PAAR4 pathway for TseMt delivery. Together, the work provides the first functional validation of P. aeruginosa H4-T6SS and mechanistic analysis of one of its effectors.
September 4, 2026 at 1:55 AM
Genetics and structural modeling indicate a dedicated DUF4123 chaperone-VgrG4b-PAAR4 pathway for TseMt delivery. Together, the work provides the first functional validation of P. aeruginosa H4-T6SS and mechanistic analysis of one of its effectors.
Secretome and competition analyses identify TseMt as a major H4-T6SS antibacterial effector. TseMt acts in the periplasm, binds membranes, and forms ion-conducting pores; its cognate immunity protein TsiMt protects target cells.
September 4, 2026 at 1:55 AM
Secretome and competition analyses identify TseMt as a major H4-T6SS antibacterial effector. TseMt acts in the periplasm, binds membranes, and forms ion-conducting pores; its cognate immunity protein TsiMt protects target cells.
A 3.0 Å cryo-EM structure shows TseMt in a compact three-domain state, with membrane-interacting elements buried inside the toxin domain. The data support a conformational switch that exposes these elements for membrane insertion and pore formation.
September 4, 2026 at 1:55 AM
A 3.0 Å cryo-EM structure shows TseMt in a compact three-domain state, with membrane-interacting elements buried inside the toxin domain. The data support a conformational switch that exposes these elements for membrane insertion and pore formation.
1/ Cover story: H4-T6SS effector TseMt
In this issue’s cover story, Wu et al. identify TseMt as a pore-forming antibacterial effector in clinical Pseudomonas aeruginosa, revealing its structure, toxin–immunity pair and delivery pathway.
doi.org/10.1002/mlf2...
In this issue’s cover story, Wu et al. identify TseMt as a pore-forming antibacterial effector in clinical Pseudomonas aeruginosa, revealing its structure, toxin–immunity pair and delivery pathway.
doi.org/10.1002/mlf2...
Functional characterization of a pore‐forming effector TseMt from the H4 type VI secretion system of <i>Pseudomonas aeruginosa</i>
Pseudomonas aeruginosa is a major nosocomial pathogen in which the type VI secretion system (T6SS) contributes to interbacterial competition and virulence. While most strains encode three T6SSs, addi...
doi.org
September 3, 2026 at 7:46 AM
1/ Cover story: H4-T6SS effector TseMt
In this issue’s cover story, Wu et al. identify TseMt as a pore-forming antibacterial effector in clinical Pseudomonas aeruginosa, revealing its structure, toxin–immunity pair and delivery pathway.
doi.org/10.1002/mlf2...
In this issue’s cover story, Wu et al. identify TseMt as a pore-forming antibacterial effector in clinical Pseudomonas aeruginosa, revealing its structure, toxin–immunity pair and delivery pathway.
doi.org/10.1002/mlf2...
Functional characterization of a pore-forming effector TseMt from the H4 type VI secretion system of Pseudomonas aeruginosa pubmed.ncbi.nlm.nih.gov/42662236/ #cryoem
August 30, 2026 at 2:38 AM
Functional characterization of a pore-forming effector TseMt from the H4 type VI secretion system of Pseudomonas aeruginosa pubmed.ncbi.nlm.nih.gov/42662236/ #cryoem