Communications Biology - ARCANE: ‘A Reprogrammable Circuit Architecture for Non-model Engineering’ enables sophisticated metabolic control in Yarrowia lipolytica

Engineered the plant-colonizing yeast Yarrowia lipolytica to produce and deliver double-stranded RNAs targeting key genes of the gray mold fungus Botrytis cinerea produced a ‘living factory’ that con...

Read the full paper: Identification and Construction of Strong Promoters in Yarrowia lipolytica Suitable for Glycerol-Based Bioprocesses

Read the full paper: Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica

This study investigated the capabilities of Yarrowia lipolytica strains to grow in media with different hydrophobic wastes from the meat industry. The yeast growth, cellular lipid accumulation, production of lipases, and degree of utilization of liquid and solid lipid wastes were studied in shaken cultures in media with organic and inorganic nitrogen sources. The effects of the type of waste, initial concentration of carbon source, Yarrowia strain, and inoculum size were investigated in two experimental sets using the Latin Square 5 × 5 design method. Post-frying rapeseed oil from chicken frying was selected as the carbon source to promote biomass growth. In contrast, the solid lipid fraction from meat broths promoted efficient lipid accumulation and yeast lipolytic activity. An initial concentration of the carbon source at 8% m/v stimulated efficient lipid biosynthesis and lipase production, while 2.5% v/v inoculum provided optimal conditions for the growth and utilization of hydrophobic substrates. No significant differences were observed in the particle dispersion of the liquid and solid wastes in the culture media (span = 2.51–3.23). The maximum emulsification index (62%) was observed in the culture of the Y. lipolytica KKP 323 strain in the medium with post-frying rapeseed oil from chicken frying, which was correlated with biosurfactant synthesis. It was concluded that the type of waste, its structure, and its composition affected various physiological yeast responses.

Published in Biotechnology journal

Published in Synthetic and systems biotechnology

Published in Bioresource technology

Published in Synthetic and systems biotechnology

Published in Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Published in Synthetic and systems biotechnology