Sporomusa ovata, a cytochrome-containing, acetogenic bacterium, conserves energy by reduction of carbon dioxide to acetate. Nitrate serves as additional electron acceptor and is reduced to ammonium, ...

Integration of phylogenetics, comparative genomics and palaeobiological approaches suggests that the last universal common ancestor lived about 4.2 billion years ago and was a complex prokaryote-grade...

Global warming driven by carbon emissions makes transitioning to a circular carbon economy increasingly urgent. Methanol is a promising energy carrier and a favorable substrate for biotechnology appli...

The thermophilic acetogenic bacterium Moorella thermoacetica is a promising platform organism to convert C1 compounds to valuable products. Gaseous C1 substrates are technically difficult to use for ...

This work presents the physiological exploration of the halo-alkaliphilic, carboxydotrophic acetogen bacterium MD4. In contrast to well-characterised neutrophilic and slightly acidophilic acetogenic ....

Background Due to its ability to grow fast on CO2, CO and H2 at high temperatures and with high energy efficiency, the thermophilic acetogen Thermoanaerobacter kivui could become an attractive host fo...

Elevated serum levels of trimethylamine N-oxide (TMAO) are reported to promote the development of atherosclerosis. TMAO is produced by hepatic oxidati…

Carbon monoxide (CO) present in syngas severely inhibits growth of many acetogens. Here, the authors adapt, characterize, and engineer utilization of CO as sole carbon and energy source by the thermophilic acetogen Thermoanaerobacter kivui.

Sporomusa ovata, a cytochrome-containing, acetogenic bacterium, conserves energy by reduction of carbon dioxide to acetate. Nitrate serves as additional electron acceptor and is reduced to ammonium, ...

Eubacterium limosum is a Clostridial acetogen that efficiently utilizes a wide range of single-carbo

Integration of phylogenetics, comparative genomics and palaeobiological approaches suggests that the last universal common ancestor lived about 4.2 billion years ago and was a complex prokaryote-grade...

In the ancient microbial Wood-Ljungdahl pathway, carbon dioxide (CO2) is fixed in a multistep process that ends with acetyl–coenzyme A (acetyl-CoA) synthesis at the bifunctional carbon monoxide dehydr...

Integration of phylogenetics, comparative genomics and palaeobiological approaches suggests that the last universal common ancestor lived about 4.2 billion years ago and was a complex prokaryote-grade anaerobic acetogen that was part of an ecosystem.

Acetogens are key biocatalysts for carbon-neutral biorefineries, yet their metabolic engineering is limited by the lack of tunable transcriptional regulation systems. Here, we developed a synthetic...

The anaerobic acetogen Acetobacterium woodii is a promising candidate producing chemicals while converting the greenhouse gas CO2. In this study, A. woodii was genetically engineered to express a lact...

Species of the genus Blautia are commonly found in the human gut and are known to be beneficial for the human well-being. However, only little is known about the physiology and the specific role of...

Chloromethane, a toxic gas primarily produced naturally, contributes to stratospheric ozone destruction. The anaerobic acetogen Acetobacterium dehalogenans can utilise chloromethane as a carbon and energy source, but the associated dehalogenase/methyltransferase has remained elusive. Through comparative transcriptomics we identify a gene cluster, cdmBCA, which encodes a corrinoid-dependent methyltransferase system distinct from the characterised Cmu system used for chloromethane degradation in aerobic methylotrophs. Biochemical characterisation reveals that the Cdm system reacts with other haloalkanes, but not with methoxylated aromatics, unlike closely related O-demethylases. X-ray structural analysis of the protein CdmB shows a hydrophobic channelling system directing haloalkanes towards cobalamin-dependent activation. Homologous proteins are found in anaerobic prokaryotes, particularly within the phyla Bacillota and Asgardarchaeota, suggesting previously unidentified microbial trans