#divisome
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stcmicrobeblog.bsky.social
all divisome fans take note 👇
#MicroSky
pinholab.bsky.social Mariana Pinho @pinholab.bsky.social · May 31
Check out this tour de force by Nils Meiresonne and colleagues, establishing FLIM-FRET as a reliable way to study protein interactions in live bacteria with spatiotemporal resolution! We open the door to exploring divisome protein interactions during cell division.

www.biorxiv.org/content/10.1...
FtsW protein-protein interactions visualized in live Staphylococcus aureus cells by FLIM-FRET
The bacterial cell cycle relies on the coordinated and dynamic interactions between division proteins and those involved in peptidoglycan (PG) synthesis. However, visualizing these interactions in viv...
www.biorxiv.org
May 31, 2025 at 11:07 AM
bactophile.bsky.social
In spite of the daily deluge of devastating news about science/the world, I’m excited to preprint some of my postdoc work (supported by an IRACDA grant-now terminated). Probing PBP1 function we uncovered unexpected features of the #Cdif #divisome 😄 We think it’s divisome could be a great drug target
biorxiv-microbiol.bsky.social bioRxiv Microbiology @biorxiv-microbiol.bsky.social · May 30
Molecular dissection of Class A PBP function uncovers novel features of the non-canonical Clostridioides difficile divisome complex https://www.biorxiv.org/content/10.1101/2025.05.29.656762v1
June 4, 2025 at 11:44 PM
archaellum.bsky.social
New preprint! Cell division has to happen at the right place but how Archaea pick that place is unknown. We now identified a three-protein system, Dip, that positions the divisome at midcell in H. volcanii and is broadly conserved across Archaea. A thread 🧵
www.biorxiv.org/content/10.6...
www.biorxiv.org
May 20, 2026 at 9:52 AM
jcamthrash.bsky.social
A conserved oscillatory system that positions the divisome in Archaea www.biorxiv.org/content/10.6... #jcampubs
May 20, 2026 at 2:33 PM
biorxiv-microbiol.bsky.social
A conserved oscillatory system that positions the divisome in Archaea https://www.biorxiv.org/content/10.64898/2026.05.19.726155v1
May 20, 2026 at 3:16 AM
biorxiv-microbiol.bsky.social
Defining the order of assembly of the Clostridioides difficile divisome complex https://www.biorxiv.org/content/10.64898/2026.01.19.700290v1
January 20, 2026 at 2:17 AM
felixrl.bsky.social
Beautiful work about a divisome positioning in Archaea
archaellum.bsky.social Sonja-Verena Albers @archaellum.bsky.social · May 20
New preprint! Cell division has to happen at the right place but how Archaea pick that place is unknown. We now identified a three-protein system, Dip, that positions the divisome at midcell in H. volcanii and is broadly conserved across Archaea. A thread 🧵
www.biorxiv.org/content/10.6...
www.biorxiv.org
May 20, 2026 at 7:00 PM
seamusholden.bsky.social
Very interesting paper on structural biology and biochemistry of Staph aureus FtsW:PBP1 (PBP1 is the divisome bPBP partner). Amongst other things, looks like some very interesting results on interaction of PBP1 PASTA domains with peptidoglycan pmc.ncbi.nlm.nih.gov/articles/PMC... #MicroSky
Integrative structural biology of the penicillin-binding protein-1 from Staphylococcus aureus, an essential component of the divisome machinery
Keywords: Divisome, Staphylococcus aureus, X-ray crystal structure, SAXS in-solution structure, Molecular dynamics simulations, PBP1, PASTA domains, Antibiotics inhibition, Peptidoglycan binding
pmc.ncbi.nlm.nih.gov
July 7, 2025 at 3:49 PM
smitslab.bsky.social
Fantastic new work from @bactophile.bsky.social @shailab.bsky.social et al from the Shen lab - Defining the order of assembly of the Clostridioides difficile divisome complex www.biorxiv.org/content/10.6... #modelnotmodel
www.biorxiv.org
January 20, 2026 at 8:44 AM
evgenii-protasov.bsky.social
Defining the order of assembly of the Clostridioides difficile divisome complex
#microbiology #Clostridia #MicroSky
@asm.org
journals.asm.org/doi/10.1128/...
Defining the order of assembly of the Clostridioides difficile divisome complex | Journal of Bacteriology
Bacterial cell division has been extensively studied in model systems, but little is known about how this essential process occurs in the clinically important pathogen Clostridioides difficile. Studie...
journals.asm.org
September 7, 2026 at 7:09 AM
stcmicrobeblog.bsky.social
(bacterial) cell divisionistas take note 👇

PG hydrolysis promotes a second wave of transpeptidation to achieve cell separation following septation in B. subtilis
#MicroSky
Fig. 7: Schematic representation of cell division in B. subtilis. Left: Cell division is mediated by the FtsZ-associated divisome complex, which initiates membrane invagination and synthesis of septal PG. PG precursors (Lipid II) are transported from the cytoplasm to the division site. The synthesis of septal PG is catalyzed by divisome-associated penicillin binding proteins (PBPs) which possess glycosyltransferase and transpeptidase activities. Middle: Once septum formation is complete, the FtsZ ring disassembles. The newly synthesized septum is initially enriched in uncrosslinked pentapeptide side-chains. The endopeptidase LytF is secreted into the periplasm and recruited to the site of cell separation. Right: The endopeptidase activity of LytF breaks down peptide bonds between parallel layers of PG to eventually form the poles of the two nascent daughter cells. Our data show that PG hydrolysis by LytF triggers post-septational transpeptidation by PBPH to facilitate the maturation of PG at the nascent cell poles. PBPH, which is recruited to the division site during septation, remains localized at the septum throughout cell separation. Created in BioRender. Patel, V. (2026) https://BioRender.com/v15i294
February 18, 2026 at 10:17 PM
pinholab.bsky.social
Check out this tour de force by Nils Meiresonne and colleagues, establishing FLIM-FRET as a reliable way to study protein interactions in live bacteria with spatiotemporal resolution! We open the door to exploring divisome protein interactions during cell division.

www.biorxiv.org/content/10.1...
FtsW protein-protein interactions visualized in live Staphylococcus aureus cells by FLIM-FRET
The bacterial cell cycle relies on the coordinated and dynamic interactions between division proteins and those involved in peptidoglycan (PG) synthesis. However, visualizing these interactions in viv...
www.biorxiv.org
May 31, 2025 at 6:57 AM
pacyc184.bsky.social
A conserved oscillatory system that positions the divisome in Archaea | bioRxiv https://www.biorxiv.org/content/10.64898/2026.05.19.726155v1?rss=1
May 20, 2026 at 4:21 AM
ameliarandich.bsky.social
We explore the differences in the early divisome between E. coli and current proteobacterial systems and argue that core functions and not core genes are generally conserved. In fact, using gene conservation and essentiality is problematic and may distort our understanding.
Venn diagram outlining how different early divisome components can share the four core functions of the early divisome in the proteobacteria.
September 25, 2023 at 12:04 PM
weiwaer.bsky.social
My first story about Archaea cell division comes out !!! A three protein system (Dip system) positions H. volcanii divisome to midcell. Dip system works like bacterial Min sytem, but they share NO sequence homology "All roads lead to Rome😊".
archaellum.bsky.social Sonja-Verena Albers @archaellum.bsky.social · May 20
New preprint! Cell division has to happen at the right place but how Archaea pick that place is unknown. We now identified a three-protein system, Dip, that positions the divisome at midcell in H. volcanii and is broadly conserved across Archaea. A thread 🧵
www.biorxiv.org/content/10.6...
www.biorxiv.org
May 20, 2026 at 3:14 PM
evolvedbiofilm.bsky.social
Minimization of the Bacillus subtilis divisome suggests FtsZ and SepF can form an active Z-ring, and reveals the amino acid transporter BraB as a new cell division influencing factor

#PLoSGenetics from Leendert Hamoen

journals.plos.org/plosgenetics...
Minimization of the Bacillus subtilis divisome suggests FtsZ and SepF can form an active Z-ring, and reveals the amino acid transporter BraB as a new cell division influencing factor
Author summary One of the key aims of synthetic biology is the construction of a minimal cell. Since bacteria are the simplest life forms, they are the preferred blueprint for such a cell. However, a ...
journals.plos.org
January 28, 2025 at 11:57 AM
biorxiv-synthbio.bsky.social
Division of Synthetic Cells Using a Genomically Encoded One-Protein Divisome https://www.biorxiv.org/content/10.64898/2026.07.21.739846v1
July 22, 2026 at 8:12 PM
bactophile.bsky.social
Excited to share my latest postdoctoral work in the Shen Lab at Tufts! In this study, we follow up on an exciting finding by former grad student @shailab.bsky.social that C diff uses its Class A PBP (PBP1) to drive cell division (unlike previously studied bacteria)! journals.plos.org/plosgenetics...
Molecular dissection of Class A PBP function uncovers novel features of the non-canonical Clostridioides difficile divisome complex
Author summary Bacterial cell division is an ancient and essential process, but our molecular understanding of this process is primarily based on studies in a select few model systems. Recent work fou...
journals.plos.org
October 28, 2025 at 1:25 AM
biorxiv-microbiol.bsky.social
Molecular dissection of Class A PBP function uncovers novel features of the non-canonical Clostridioides difficile divisome complex https://www.biorxiv.org/content/10.1101/2025.05.29.656762v1
May 30, 2025 at 5:17 AM
ameliarandich.bsky.social
Wake up in the dead of night wondering whether deviations in gene conservation and/or essentiality between your non-model bacterium and E. coli make it irrelevant? Gustavo, Pam JB Brown, and I tackle this issue in the early divisome in our new opinion piece in TiM: tinyurl.com/2tdma4tu 🦠 🧫 #microsky
September 25, 2023 at 12:01 PM
bactophile.bsky.social
Gotta say that I’m extra proud of this team effort out in JB! Our understanding of Cdif division is in its infancy & there’s so much work to be done. Shoutout to @shailab.bsky.social for laying the foundation & starting this cool project with @polakuhn.bsky.social!! journals.asm.org/doi/10.1128/...
Defining the order of assembly of the Clostridioides difficile divisome complex | Journal of Bacteriology
Bacterial cell division has been extensively studied in model systems, but little is known about how this essential process occurs in the clinically important pathogen Clostridioides difficile. Studie...
journals.asm.org
August 27, 2026 at 3:02 PM
pacyc184.bsky.social
A conserved oscillatory system that positions the divisome in Archaea | bioRxiv https://www.biorxiv.org/content/10.64898/2026.05.19.726155v1
May 21, 2026 at 12:13 AM
biorxiv-microbiol.bsky.social
Structure-guided generative design of peptides targeting the FtsQBL divisome complex inhibit Escherichia coli cell division. https://www.biorxiv.org/content/10.64898/2026.02.27.708549v1
March 2, 2026 at 2:16 AM
avettiger.bsky.social
It has been coming a long way... happy to share our latest work with you: www.biorxiv.org/content/10.1...
Here, we show the importance of septal fortification by the Class A Penicillin Binding Protein, PBP1b, in E. coli and provide a molecular mechanism for this function. (1/12).
The aPBP-type cell wall synthase PBP1b plays a specialized role in fortifying the Escherichia coli division site against osmotic rupture
A multi-protein system called the divisome promotes bacterial division. This apparatus synthesizes the peptidoglycan (PG) cell wall layer that forms the daughter cell poles and protects them from osmo...
www.biorxiv.org
April 3, 2025 at 10:13 PM
ceesdekker.bsky.social
Latest #CDlab paper: a review entitled 'Devising a divisome for synthetic cells' in Nature Reviews Chemistry
www.nature.com/articles/s41...

Here, 1st authors Joris Dommisse and Rafael Lira review current strategies to mimic division: symmetry breaking, constriction and abscission.
Enjoy the read!
July 13, 2026 at 5:48 PM