Happy #FluorescenceFriday. eSRRF reconstruction of actin dynamics in PMA-stimulated macrophages. Scale bar is 2 microns!
September 25, 2026 at 6:46 PM
Happy #FluorescenceFriday. eSRRF reconstruction of actin dynamics in PMA-stimulated macrophages. Scale bar is 2 microns!
It's here! ✨
The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF
➡️ Open link: rdcu.be/euvEw
The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF
➡️ Open link: rdcu.be/euvEw
July 3, 2025 at 8:44 AM
It's here! ✨
The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF
➡️ Open link: rdcu.be/euvEw
The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF
➡️ Open link: rdcu.be/euvEw
July 3, 2025 at 8:05 AM
SoLo, a source localization algorithm for super-resolution that works with either single images or fluctuating time series (a la eSRRF): arxiv.org/abs/2604.20248
Github code: github.com/M-R-Wang/SoLo
Github code: github.com/M-R-Wang/SoLo
April 23, 2026 at 4:53 PM
SoLo, a source localization algorithm for super-resolution that works with either single images or fluctuating time series (a la eSRRF): arxiv.org/abs/2604.20248
Github code: github.com/M-R-Wang/SoLo
Github code: github.com/M-R-Wang/SoLo
Over the moon to see #eSRRF out! Another chapter in our great collaboration with @henriqueslab.bsky.social and all the gang. Special shoutout to NeuroCyto postdoc @terrymosoly who used #eSRRF to super-resolve microtubules in living neurons!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
🧵Have you ever tried #SRRF? Yes… then you are gonna 😍 this! No?… What took you so long? 😁 Finally #eSRRF is published @naturemethods! We are taking live-cell 3D #superresolution to the next level🏄♀️ 🔬: www.nature.com/articles/s41...
November 13, 2023 at 5:18 PM
Over the moon to see #eSRRF out! Another chapter in our great collaboration with @henriqueslab.bsky.social and all the gang. Special shoutout to NeuroCyto postdoc @terrymosoly who used #eSRRF to super-resolve microtubules in living neurons!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
🚨 Important detail in the #ExM & #eSRRF protocol: #eSRRF is not only available as plugin in #ImageJ /Fiji 🌴, #NanoPyx also brings it into the #python world 🐍: doi.org/10.1038/s415...
How to get started? Check out the training manual: doi.org/10.5281/zeno...
How to get started? Check out the training manual: doi.org/10.5281/zeno...
July 4, 2025 at 7:57 AM
🚨 Important detail in the #ExM & #eSRRF protocol: #eSRRF is not only available as plugin in #ImageJ /Fiji 🌴, #NanoPyx also brings it into the #python world 🐍: doi.org/10.1038/s415...
How to get started? Check out the training manual: doi.org/10.5281/zeno...
How to get started? Check out the training manual: doi.org/10.5281/zeno...
Exciting news! 🎉 I’m thrilled to join the @pnordenfelt.bsky.social Lab @lunduniversity-med.bsky.social as a postdoc! I’ll be working on bacterial infections using #DDM and #SuperResolution imaging, uncovering new insights into host-pathogen interactions. 🔬🦠 #PostdocLife #Microbiology #eSRRF 🏄♀️
February 6, 2025 at 4:20 PM
Exciting news! 🎉 I’m thrilled to join the @pnordenfelt.bsky.social Lab @lunduniversity-med.bsky.social as a postdoc! I’ll be working on bacterial infections using #DDM and #SuperResolution imaging, uncovering new insights into host-pathogen interactions. 🔬🦠 #PostdocLife #Microbiology #eSRRF 🏄♀️
New enhanced SRRF (eSRRF) and 3D eSRRF using multifocal microscopy, from the @henriqueslab.bsky.social lab! www.nature.com/articles/s41...
November 14, 2023 at 2:10 AM
New enhanced SRRF (eSRRF) and 3D eSRRF using multifocal microscopy, from the @henriqueslab.bsky.social lab! www.nature.com/articles/s41...
Happy #FluorescenceFriday! This is the cortical actin cytoskeleton of a macrophage imaged using eSRRF and @spirochrome.com's SPY555-FastAct_X.
August 28, 2026 at 5:19 PM
Happy #FluorescenceFriday! This is the cortical actin cytoskeleton of a macrophage imaged using eSRRF and @spirochrome.com's SPY555-FastAct_X.
Nice intro to combining SRRF with expansion microscopy (eSRRF), check it out ⬇️
July 3, 2025 at 12:18 PM
Nice intro to combining SRRF with expansion microscopy (eSRRF), check it out ⬇️
I‘m already on my way to Prag for @embo.org #SLM2025 #Super-resolution practical course! Looking forward to contribute with live-cell #eSRRF 🏄♀️ , meet some familiar faces and many new ones 🤩. Here some impressions from the 🚂: Öresund, Berlin, Dresden & the Bastei.
June 11, 2025 at 6:11 PM
I‘m already on my way to Prag for @embo.org #SLM2025 #Super-resolution practical course! Looking forward to contribute with live-cell #eSRRF 🏄♀️ , meet some familiar faces and many new ones 🤩. Here some impressions from the 🚂: Öresund, Berlin, Dresden & the Bastei.
🚨🔬🤖📜 New paper! Introducing #LiquidEngine and #NanoPyx - #AI for accelerating #microscopy that explores how to maximise performance 🚀. Brainchild of
Bruno, @inesmcunha.bsky.social and António. Adventure with @guijacquemet.bsky.social et al. Faster #SRRF & #eSRRF!!
📄: www.nature.com/articles/s41...
Bruno, @inesmcunha.bsky.social and António. Adventure with @guijacquemet.bsky.social et al. Faster #SRRF & #eSRRF!!
📄: www.nature.com/articles/s41...
January 2, 2025 at 11:51 AM
🚨🔬🤖📜 New paper! Introducing #LiquidEngine and #NanoPyx - #AI for accelerating #microscopy that explores how to maximise performance 🚀. Brainchild of
Bruno, @inesmcunha.bsky.social and António. Adventure with @guijacquemet.bsky.social et al. Faster #SRRF & #eSRRF!!
📄: www.nature.com/articles/s41...
Bruno, @inesmcunha.bsky.social and António. Adventure with @guijacquemet.bsky.social et al. Faster #SRRF & #eSRRF!!
📄: www.nature.com/articles/s41...
November 14, 2023 at 3:46 AM
🧵Have you ever tried #SRRF? Yes… then you are gonna 😍 this! No?… What took you so long? 😁 Finally #eSRRF is published @naturemethods! We are taking live-cell 3D #superresolution to the next level🏄♀️ 🔬: www.nature.com/articles/s41...
November 13, 2023 at 4:30 PM
🧵Have you ever tried #SRRF? Yes… then you are gonna 😍 this! No?… What took you so long? 😁 Finally #eSRRF is published @naturemethods! We are taking live-cell 3D #superresolution to the next level🏄♀️ 🔬: www.nature.com/articles/s41...
And there is an extra training manual on eSRRF processing 🏋️https://zenodo.org/doi/10.5281/zenodo.11518140
Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues - Data processing manual
Here we provide test datasets and a training manual for the parameter optimization with eSRRF. The training manual will guide users through an eSRRF paramenter optimization routine and quantiative im...
zenodo.org
July 3, 2025 at 8:05 AM
And there is an extra training manual on eSRRF processing 🏋️https://zenodo.org/doi/10.5281/zenodo.11518140
Delighted to have contributed to #eSRRF ! Beautiful and incredibly useful work by
@henriqueslab.bsky.social
. Congratulations to
@hannah-superres.bsky.social
Romain Laine and everyone else involved 🚀🚀🔬🔬
@henriqueslab.bsky.social
. Congratulations to
@hannah-superres.bsky.social
Romain Laine and everyone else involved 🚀🚀🔬🔬
🧵Have you ever tried #SRRF? Yes… then you are gonna 😍 this! No?… What took you so long? 😁 Finally #eSRRF is published @naturemethods! We are taking live-cell 3D #superresolution to the next level🏄♀️ 🔬: www.nature.com/articles/s41...
November 13, 2023 at 4:38 PM
Delighted to have contributed to #eSRRF ! Beautiful and incredibly useful work by
@henriqueslab.bsky.social
. Congratulations to
@hannah-superres.bsky.social
Romain Laine and everyone else involved 🚀🚀🔬🔬
@henriqueslab.bsky.social
. Congratulations to
@hannah-superres.bsky.social
Romain Laine and everyone else involved 🚀🚀🔬🔬
🚨🔬💻 Future of SRRF is here! eSRRF considerably improves image quality by data-driven parameter optimization, bringing full #3DSR. Thanks to the amazing work of Romain Laine, @hannah-superres.bsky.social together with @christlet.bsky.social, @guijacquemet.bsky.social, Hajj labs and colleagues
🧵Have you ever tried #SRRF? Yes… then you are gonna 😍 this! No?… What took you so long? 😁 Finally #eSRRF is published @naturemethods! We are taking live-cell 3D #superresolution to the next level🏄♀️ 🔬: www.nature.com/articles/s41...
November 13, 2023 at 5:15 PM
🚨🔬💻 Future of SRRF is here! eSRRF considerably improves image quality by data-driven parameter optimization, bringing full #3DSR. Thanks to the amazing work of Romain Laine, @hannah-superres.bsky.social together with @christlet.bsky.social, @guijacquemet.bsky.social, Hajj labs and colleagues
Yes, we made a video tutorial, have a look at github.com/HenriquesLab...
GitHub - HenriquesLab/NanoJ-eSRRF
Contribute to HenriquesLab/NanoJ-eSRRF development by creating an account on GitHub.
github.com
November 15, 2023 at 6:39 AM
Yes, we made a video tutorial, have a look at github.com/HenriquesLab...
In modern microscopy, scaling data often leads to heavy computational loads. NanoPyx uses Liquid Engine to adapt CPU/GPU code on the fly, accelerating tasks like eSRRF or NLM denoising up to 24×. It's open-source, data-driven, & a robust choice for advanced bioimaging. www.nature.com/articles/s41...
Efficiently accelerated bioimage analysis with NanoPyx, a Liquid Engine-powered Python framework - Nature Methods
NanoPyx optimizes bioimage analysis workflows by dynamically selecting code variations based on input data and hardware. This ensures maximum computational efficiency, enabling fast analysis of large ...
www.nature.com
January 2, 2025 at 2:23 PM
In modern microscopy, scaling data often leads to heavy computational loads. NanoPyx uses Liquid Engine to adapt CPU/GPU code on the fly, accelerating tasks like eSRRF or NLM denoising up to 24×. It's open-source, data-driven, & a robust choice for advanced bioimaging. www.nature.com/articles/s41...
Microscopes come in all flavours 🌶️, we did our best to try many 🔬! A shoutout to the #eSRRF team 🌍: Simao Coelho, Damian Martinez, @HenriquesLab.bsky.social 🇵🇹, Theresa Wiesner, Angelique Jimenez, @christlet.bsky.social 🇫🇷, Jonathan Nixon-Abel 🇬🇧🇺🇸,…
November 13, 2023 at 4:58 PM
Microscopes come in all flavours 🌶️, we did our best to try many 🔬! A shoutout to the #eSRRF team 🌍: Simao Coelho, Damian Martinez, @HenriquesLab.bsky.social 🇵🇹, Theresa Wiesner, Angelique Jimenez, @christlet.bsky.social 🇫🇷, Jonathan Nixon-Abel 🇬🇧🇺🇸,…
Reminds me of the increasing density PAINT dataset we made for @henriqueslab.bsky.social to test eSRRF against SMLM (www.nature.com/articles/s41...)
November 12, 2024 at 4:06 PM
Reminds me of the increasing density PAINT dataset we made for @henriqueslab.bsky.social to test eSRRF against SMLM (www.nature.com/articles/s41...)
Too much or too little 🤷 ❓Getting the best of 🤝 image fidelity✨ & resolution 📌, is a matter of balance in super-resolution microscopy ⚖️🧘♀️🔬… and the cause of🧑🏻🔬👉 👨🦳. #eSRRF ’s data-driven parameter optimization comes to the rescue🛟 t.co/TY3o4xZPzI
Too much or too little? Pursuing the optimal balance in super-resolution microscopy.
t.co
November 14, 2023 at 10:51 PM
Too much or too little 🤷 ❓Getting the best of 🤝 image fidelity✨ & resolution 📌, is a matter of balance in super-resolution microscopy ⚖️🧘♀️🔬… and the cause of🧑🏻🔬👉 👨🦳. #eSRRF ’s data-driven parameter optimization comes to the rescue🛟 t.co/TY3o4xZPzI
Congrats! Just reading now. Hope to try it out (at least the eSRRF part... we are not setup for mutifocal).
November 14, 2023 at 2:11 AM
Congrats! Just reading now. Hope to try it out (at least the eSRRF part... we are not setup for mutifocal).
Amazing talk by @HenriquesLab ! Fully convinced to try #eSRRF on some live cell SAF imaging …!
x.com/HenriquesLab/s…
x.com/HenriquesLab/s…
January 14, 2025 at 3:03 PM
Amazing talk by @HenriquesLab ! Fully convinced to try #eSRRF on some live cell SAF imaging …!
x.com/HenriquesLab/s…
x.com/HenriquesLab/s…