Nature research paper: MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness
go.nature.com/4AwjkKA
go.nature.com/4AwjkKA
MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness - Nature
Stability of extrachromosomal DNA (ecDNA) relies on microhomology-mediated end joining at fragile TA-rich sites, with FANCM suppressing break formation, suggesting that Polθ disruption may destabilize ecDNA and sensitize ecDNA-driven tumours to therapeutic intervention.
go.nature.com
September 26, 2026 at 11:34 AM
Nature research paper: MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness
go.nature.com/4AwjkKA
go.nature.com/4AwjkKA
Here, we show that the unique regulatory landscape of ecDNA enables an ancient LINE to resurrect and act as an enhancer of Myc. This was so fun with @katerinakraft.bsky.social and @mattjones.bsky.social and others. www.nature.com/articles/s41...
Enhancer activation from transposable elements in extrachromosomal DNA - Nature Cell Biology
Kraft, Murphy, Jones et al. identify extrachromosomal DNA (ecDNA)-interacting elements (EIEs) enriched for transposable elements within ecDNA in colorectal cancer cells. They show that EIE 14 integrat...
www.nature.com
October 21, 2025 at 9:43 AM
Here, we show that the unique regulatory landscape of ecDNA enables an ancient LINE to resurrect and act as an enhancer of Myc. This was so fun with @katerinakraft.bsky.social and @mattjones.bsky.social and others. www.nature.com/articles/s41...
Very nice paper from @agnelsfeir.bsky.social and colleagues on the role of MMEJ in ecDNA maintenance and rearrangements.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness - Nature
Stability of extrachromosomal DNA (ecDNA) relies on microhomology-mediated end joining at fragile TA-rich sites, with FANCM suppressing break formation, suggesting that Polθ disruption may destab...
www.nature.com
September 25, 2026 at 1:47 PM
Very nice paper from @agnelsfeir.bsky.social and colleagues on the role of MMEJ in ecDNA maintenance and rearrangements.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Please check out our preprint where we find that mitotic transcription helps ensure ecDNA inheritance through chromosomal tethering:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
February 17, 2025 at 11:04 AM
Please check out our preprint where we find that mitotic transcription helps ensure ecDNA inheritance through chromosomal tethering:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
☕ @katerinakraft.bsky.social @sedonamurphy.bsky.social @mattjones.bsky.social et al. identify extrachromosomal DNA(ecDNA)-interacting elements (EIEs) enriched for #TransposableElements in #ColorectalCancer. EIE 14 integrated within ecDNA acts as an enhancer to promote cancer fitness.
bit.ly/49iFC7C
bit.ly/49iFC7C
Enhancer activation from transposable elements in extrachromosomal DNA - Nature Cell Biology
Kraft, Murphy, Jones et al. identify extrachromosomal DNA (ecDNA)-interacting elements (EIEs) enriched for transposable elements within ecDNA in colorectal cancer cells. They show that EIE 14 integrat...
bit.ly
November 16, 2025 at 8:29 AM
☕ @katerinakraft.bsky.social @sedonamurphy.bsky.social @mattjones.bsky.social et al. identify extrachromosomal DNA(ecDNA)-interacting elements (EIEs) enriched for #TransposableElements in #ColorectalCancer. EIE 14 integrated within ecDNA acts as an enhancer to promote cancer fitness.
bit.ly/49iFC7C
bit.ly/49iFC7C
What a joy it was hosting ZZ and hearing his amazing work on transposons and ecDNA!
Thank you to Zhao Zhang (@duke-university.bsky.social) for joining us for a #StowersSeminarSeries talk, hosted by the @kkostova.bsky.social Lab!
September 28, 2026 at 9:17 PM
What a joy it was hosting ZZ and hearing his amazing work on transposons and ecDNA!
Nature research paper: MYC ecDNA promotes intratumour heterogeneity and plasticity in PDAC
https://go.nature.com/41IPJwX
https://go.nature.com/41IPJwX
MYC ecDNA promotes intratumour heterogeneity and plasticity in PDAC - Nature
In a model of pancreatic ductal adenocarcinoma, extrachromosomal DNAs are shown to be a source of high-level focal amplification driving MYC heterogeneity and phenotypic adaptation.
go.nature.com
March 12, 2025 at 7:34 PM
Nature research paper: MYC ecDNA promotes intratumour heterogeneity and plasticity in PDAC
https://go.nature.com/41IPJwX
https://go.nature.com/41IPJwX
Our paper is out in @nature.com! Oncogenes are often copy-number amplified on extrachromosomal DNA (ecDNA) in cancer, but how is ecDNA inherited by dividing cells? Here we identified elements within ecDNA that promote its retention in dividing cells. 1/11
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Genetic elements promote retention of extrachromosomal DNA in cancer cells - Nature
A combination of genome-wide functional screening, imaging and chromatin profiling identifies a new class of highly prevalent genomic elements that help retain extrachromosomal DNA copies in dividing ...
www.nature.com
November 19, 2025 at 7:03 PM
Our paper is out in @nature.com! Oncogenes are often copy-number amplified on extrachromosomal DNA (ecDNA) in cancer, but how is ecDNA inherited by dividing cells? Here we identified elements within ecDNA that promote its retention in dividing cells. 1/11
www.nature.com/articles/s41...
www.nature.com/articles/s41...
My new lab at the @molgen.mpg.de will be following up on some of the nuclear organization aspects of ecDNA so please do reach out if this is of interest :)
October 21, 2025 at 9:43 AM
My new lab at the @molgen.mpg.de will be following up on some of the nuclear organization aspects of ecDNA so please do reach out if this is of interest :)
This week in Nature - four papers probe the relationship between ecDNA & cancer
* an atlas of ecDNA in cancer
* ecDNA inheritance in cancer during cell division
* a potential way to leverage ecDNA for treatment
* ecDNA role in tumour evolution & therapy resistance
www.nature.com/nature/volum...
* an atlas of ecDNA in cancer
* ecDNA inheritance in cancer during cell division
* a potential way to leverage ecDNA for treatment
* ecDNA role in tumour evolution & therapy resistance
www.nature.com/nature/volum...
Nature - Outside influence
The cover shows circular extrachromosomal DNA (ecDNA) sitting next to a chromosome. ecDNA has a significant effect on the outcome of cancer treatment —...
www.nature.com
November 19, 2024 at 8:54 PM
This week in Nature - four papers probe the relationship between ecDNA & cancer
* an atlas of ecDNA in cancer
* ecDNA inheritance in cancer during cell division
* a potential way to leverage ecDNA for treatment
* ecDNA role in tumour evolution & therapy resistance
www.nature.com/nature/volum...
* an atlas of ecDNA in cancer
* ecDNA inheritance in cancer during cell division
* a potential way to leverage ecDNA for treatment
* ecDNA role in tumour evolution & therapy resistance
www.nature.com/nature/volum...
Now online! Extrachromosomal DNA replication and maintenance couple with DNA damage pathway in tumors
Extrachromosomal DNA replication and maintenance couple with DNA damage pathway in tumors
This study demonstrates that extrachromosomal DNA (ecDNA) replication induces DNA double-strand breaks and activates the DNA damage response (DDR). The DDR pathways, such as alt-NHEJ, are critical for ecDNA maintenance in tumor cells. Mechanistic insights into ecDNA replication and maintenance unveil a therapeutic approach for treating tumors harboring ecDNA.
dlvr.it
April 28, 2025 at 6:51 PM
Now online! Extrachromosomal DNA replication and maintenance couple with DNA damage pathway in tumors
Delighted to share our new study in @natcellbio.nature.com showing that the unique epigenetic landscape of ecDNA can enable the oncogenic enhancer activation of transposable elements! This was a great collaboration with @katerinakraft.bsky.social and @sedonamurphy.bsky.social. tinyurl.com/mwfbu4w4
Enhancer activation from transposable elements in extrachromosomal DNA - Nature Cell Biology
Kraft, Murphy, Jones et al. identify extrachromosomal DNA (ecDNA)-interacting elements (EIEs) enriched for transposable elements within ecDNA in colorectal cancer cells. They show that EIE 14 integrat...
tinyurl.com
October 21, 2025 at 2:43 PM
Delighted to share our new study in @natcellbio.nature.com showing that the unique epigenetic landscape of ecDNA can enable the oncogenic enhancer activation of transposable elements! This was a great collaboration with @katerinakraft.bsky.social and @sedonamurphy.bsky.social. tinyurl.com/mwfbu4w4
exciting work: RNA-mediated tethering of DNA during mitosis www.biorxiv.org/content/10.1...
Mitotic transcription ensures ecDNA inheritance through chromosomal tethering
Extrachromosomal DNA (ecDNA) are circular DNA bodies that play critical roles in tumor progression and treatment resistance by amplifying oncogenes across a wide range of cancer types. ecDNA lack cent...
www.biorxiv.org
February 23, 2025 at 10:56 AM
exciting work: RNA-mediated tethering of DNA during mitosis www.biorxiv.org/content/10.1...
EcDNA-borne structural variants drive oncogenic fusion transcript amplification
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
EcDNA-borne structural variants drive oncogenic fusion transcript amplification
Extrachromosomal DNA (ecDNA) is a major source of oncogenic fusions across cancer
types, generating tissue-specific fusion landscapes with diagnostic potential. EcDNA-borne
PVT1 5′-end fusions stabili...
www.cell.com
January 7, 2026 at 7:10 PM
EcDNA-borne structural variants drive oncogenic fusion transcript amplification
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
ecDNA @theaacr.bsky.social
Paul Mischel from Stanford.
In breast cancer ecDNA is the main mechanism of amplification of the ER+ oncogene we co-discovered ZNF703!
@caldaslab.bsky.social
Paul Mischel from Stanford.
In breast cancer ecDNA is the main mechanism of amplification of the ER+ oncogene we co-discovered ZNF703!
@caldaslab.bsky.social
April 27, 2025 at 3:21 PM
ecDNA @theaacr.bsky.social
Paul Mischel from Stanford.
In breast cancer ecDNA is the main mechanism of amplification of the ER+ oncogene we co-discovered ZNF703!
@caldaslab.bsky.social
Paul Mischel from Stanford.
In breast cancer ecDNA is the main mechanism of amplification of the ER+ oncogene we co-discovered ZNF703!
@caldaslab.bsky.social
Thanks to Cancer Discovery (not on bsky yet?) for highlighting our recent publication on ecDNA amplifications during cancer progression: aacrjournals.org/cdnews/news/....
aacrjournals.org
November 25, 2024 at 9:47 PM
Thanks to Cancer Discovery (not on bsky yet?) for highlighting our recent publication on ecDNA amplifications during cancer progression: aacrjournals.org/cdnews/news/....
I am excited, our collaborative work on tumours driven by ecDNA amplified MYCN and the underlying copy-number dependent fitness landscape just came out in Cancer Discovery @aacrjournals.bsky.social.
@anton-henssen.bsky.social @cancergrand.bsky.social
aacrjournals.org/cancerdiscov...
@anton-henssen.bsky.social @cancergrand.bsky.social
aacrjournals.org/cancerdiscov...
Extrachromosomal DNA-Driven Oncogene Dosage Heterogeneity Promotes Rapid Adaptation to Therapy in MYCN-Amplified Cancers
AbstractExtrachromosomal DNA (ecDNA) amplification enhances intercellular oncogene dosage variability and accelerates tumor evolution by violating foundational principles of genetic inheritance throug...
aacrjournals.org
August 8, 2025 at 10:22 AM
I am excited, our collaborative work on tumours driven by ecDNA amplified MYCN and the underlying copy-number dependent fitness landscape just came out in Cancer Discovery @aacrjournals.bsky.social.
@anton-henssen.bsky.social @cancergrand.bsky.social
aacrjournals.org/cancerdiscov...
@anton-henssen.bsky.social @cancergrand.bsky.social
aacrjournals.org/cancerdiscov...
Very excited to share the stellar speaker line-up at our ecDNA conference this June in London! 👇
If you're interested in #ecDNA and want to hear or present groundbreaking research, register now. Abstract submission deadline is 14 March.
Learn more: www.cancergrandchallenges.org/ecDNA-2025
If you're interested in #ecDNA and want to hear or present groundbreaking research, register now. Abstract submission deadline is 14 March.
Learn more: www.cancergrandchallenges.org/ecDNA-2025
February 24, 2025 at 10:29 AM
Very excited to share the stellar speaker line-up at our ecDNA conference this June in London! 👇
If you're interested in #ecDNA and want to hear or present groundbreaking research, register now. Abstract submission deadline is 14 March.
Learn more: www.cancergrandchallenges.org/ecDNA-2025
If you're interested in #ecDNA and want to hear or present groundbreaking research, register now. Abstract submission deadline is 14 March.
Learn more: www.cancergrandchallenges.org/ecDNA-2025
This is awesome - very clever approach to investigate how ecDNA help malignant clones evolve!
🧪1/ 🚨New paper on ecDNAs from our lab!
We reveal a strategy to engineer extrachromosomal DNA (ecDNA) amplifications in cells & mice. Let's dive in! Let’s dive in! 🧵👇
www.nature.com/articles/s41...
We reveal a strategy to engineer extrachromosomal DNA (ecDNA) amplifications in cells & mice. Let's dive in! Let’s dive in! 🧵👇
www.nature.com/articles/s41...
Engineered extrachromosomal oncogene amplifications promote tumorigenesis - Nature
Large extrachromosomal DNAs are engineered using a CRISPR- and Cre–loxP-based approach and shown to drive cancer in mouse models, with potential applications in determining the role of oncogene a...
www.nature.com
December 19, 2024 at 4:54 AM
This is awesome - very clever approach to investigate how ecDNA help malignant clones evolve!
Very happy ecDNA has made the cover at @aacrjournals.bsky.social, highlighting two articles in the current issue, one on MYC(N) amplification in Neuroblastoma and one on the spatial heterogeneity of ecDNA amplified oncogenes in GBM.
aacrjournals.org/cancerdiscov...
aacrjournals.org/cancerdiscov...
Volume 15 Issue 10 | Cancer Discovery | American Association for Cancer Research
aacrjournals.org
October 6, 2025 at 9:54 AM
Very happy ecDNA has made the cover at @aacrjournals.bsky.social, highlighting two articles in the current issue, one on MYC(N) amplification in Neuroblastoma and one on the spatial heterogeneity of ecDNA amplified oncogenes in GBM.
aacrjournals.org/cancerdiscov...
aacrjournals.org/cancerdiscov...
Nice findings on ecDNA segregation. Congrats John and Ashley!
Please check out our preprint where we find that mitotic transcription helps ensure ecDNA inheritance through chromosomal tethering:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
February 17, 2025 at 11:43 PM
Nice findings on ecDNA segregation. Congrats John and Ashley!
Oh hey, looks like I can upload longer videos here now!
Here’s my most recent one covering the latest findings on ecDNA retention mechanisms from the Chang & Mischel labs.
Here’s my most recent one covering the latest findings on ecDNA retention mechanisms from the Chang & Mischel labs.
December 4, 2025 at 1:20 AM
Oh hey, looks like I can upload longer videos here now!
Here’s my most recent one covering the latest findings on ecDNA retention mechanisms from the Chang & Mischel labs.
Here’s my most recent one covering the latest findings on ecDNA retention mechanisms from the Chang & Mischel labs.
From enhancer hijacking to ecDNA and onco-condensates – we review how changes in nuclear architecture drive oncogenic gene expression by disrupting enhancer–promoter communication. doi.org/10.1002/ijc..... Kudos to Isabelle Seufert, @claire-vrgs.bsky.social and Sina Wille 👏
April 12, 2025 at 8:38 PM
From enhancer hijacking to ecDNA and onco-condensates – we review how changes in nuclear architecture drive oncogenic gene expression by disrupting enhancer–promoter communication. doi.org/10.1002/ijc..... Kudos to Isabelle Seufert, @claire-vrgs.bsky.social and Sina Wille 👏
Published today in @aacrjournals.bsky.social Imran Noorani & @magnushaughey.bsky.social share the first evidence that ecDNA can build up in the healthy brain before tumours form in #glioblastoma — pointing to new early intervention strategies.
👉 www.cancergrandchallenges.org/news/ecdna-u...
👉 www.cancergrandchallenges.org/news/ecdna-u...
September 8, 2025 at 2:26 PM
Published today in @aacrjournals.bsky.social Imran Noorani & @magnushaughey.bsky.social share the first evidence that ecDNA can build up in the healthy brain before tumours form in #glioblastoma — pointing to new early intervention strategies.
👉 www.cancergrandchallenges.org/news/ecdna-u...
👉 www.cancergrandchallenges.org/news/ecdna-u...