🧵 1/8 Have you ever wondered how information embedded within exons regulates gene expression in plants? 🌱🧬 Check out our latest work, where we explore how exonic enhancers (EEs) shape gene expression in plants! www.biorxiv.org/content/10.6...
September 24, 2026 at 5:19 AM
🧵 1/8 Have you ever wondered how information embedded within exons regulates gene expression in plants? 🌱🧬 Check out our latest work, where we explore how exonic enhancers (EEs) shape gene expression in plants! www.biorxiv.org/content/10.6...
the process of building an app is like the process of building a protein: there are the useful problem solving bits - exons - and then there are a whooole bunch more bits - introns - related to navigating whatever framework(s) you're using to make the exons possible
May 5, 2026 at 7:05 PM
the process of building an app is like the process of building a protein: there are the useful problem solving bits - exons - and then there are a whooole bunch more bits - introns - related to navigating whatever framework(s) you're using to make the exons possible
I’ve been studying gene regulation in primates for over 20 years, and using RNA-seq since 2008 to compare expression across species. We thought we were being careful. We used curated orthologous exons to minimize alignment bias and focused on biologically meaningful comparisons.
We were wrong.
We were wrong.
June 7, 2025 at 12:07 AM
I’ve been studying gene regulation in primates for over 20 years, and using RNA-seq since 2008 to compare expression across species. We thought we were being careful. We used curated orthologous exons to minimize alignment bias and focused on biologically meaningful comparisons.
We were wrong.
We were wrong.
The target wordcount for Exons was 100,000. Something nice and easy to submit to publishers.
Current wordcount? 101,221.
Is it finished, Anesha?
No. No it is not.
Current wordcount? 101,221.
Is it finished, Anesha?
No. No it is not.
September 23, 2026 at 2:58 AM
The target wordcount for Exons was 100,000. Something nice and easy to submit to publishers.
Current wordcount? 101,221.
Is it finished, Anesha?
No. No it is not.
Current wordcount? 101,221.
Is it finished, Anesha?
No. No it is not.
Bluesky Klasse aufgepasst 🧐☝️
Es wird mal wieder Zeit für ein bisschen Molekularbiologie. Heute mit dem Thema "Splicing" 🧑🔬 🧬
Teil 1: Exons und Introns 🧵 1/7
Es wird mal wieder Zeit für ein bisschen Molekularbiologie. Heute mit dem Thema "Splicing" 🧑🔬 🧬
Teil 1: Exons und Introns 🧵 1/7
April 4, 2024 at 4:58 PM
Bluesky Klasse aufgepasst 🧐☝️
Es wird mal wieder Zeit für ein bisschen Molekularbiologie. Heute mit dem Thema "Splicing" 🧑🔬 🧬
Teil 1: Exons und Introns 🧵 1/7
Es wird mal wieder Zeit für ein bisschen Molekularbiologie. Heute mit dem Thema "Splicing" 🧑🔬 🧬
Teil 1: Exons und Introns 🧵 1/7
Many conserved exons in the heart and brain utilize weak 5 ′ splice sites, yet they are accurately spliced. But how? We show that splicing fidelity is actively enforced through a QKI-U6 checkpoint at the U1→U6 handover in essential cardiac genes during organogenesis.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
QKI ensures splicing fidelity during cardiogenesis by engaging the U6 tri-snRNP to activate splicing at weak 5ʹ splice sites
During organogenesis, precise pre-mRNA splicing is essential to assemble tissue architecture. Many developmentally essential exons bear weak 5'splice sites (5'SS) yet are spliced with high precision, ...
www.biorxiv.org
September 8, 2025 at 6:12 AM
Many conserved exons in the heart and brain utilize weak 5 ′ splice sites, yet they are accurately spliced. But how? We show that splicing fidelity is actively enforced through a QKI-U6 checkpoint at the U1→U6 handover in essential cardiac genes during organogenesis.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Recommendation: One of the web serials I've been following for the past month is an apocalyptic TG virus story set in an dystopian near-future.
It's called Exons and it's by @aneshagriffiths.bsky.social
www.scribblehub.com/series/23835...
It's called Exons and it's by @aneshagriffiths.bsky.social
www.scribblehub.com/series/23835...
Exons
When Erika got a biotech job at Neuragene she thought she'd got it made. But like most things in life, it turns out it's not as good as it seemed, and the plush job came with a catch she decided she c...
www.scribblehub.com
September 22, 2026 at 4:58 PM
Recommendation: One of the web serials I've been following for the past month is an apocalyptic TG virus story set in an dystopian near-future.
It's called Exons and it's by @aneshagriffiths.bsky.social
www.scribblehub.com/series/23835...
It's called Exons and it's by @aneshagriffiths.bsky.social
www.scribblehub.com/series/23835...
Playing with #vizitig again to prepare for the 1.0 release. This is the SNAP25 human gene expressed in 3 SRA datasets linked to cancers.
Exons have one color each, and exon junctions are in yellow.
The rest is new/noise.
Exons have one color each, and exon junctions are in yellow.
The rest is new/noise.
March 26, 2025 at 8:33 PM
Playing with #vizitig again to prepare for the 1.0 release. This is the SNAP25 human gene expressed in 3 SRA datasets linked to cancers.
Exons have one color each, and exon junctions are in yellow.
The rest is new/noise.
Exons have one color each, and exon junctions are in yellow.
The rest is new/noise.
How can a splicing factor bind to thousands of exons but regulate only a few of them? In our new preprint, we show that SRSF6 resolves this paradox through two distinct binding modes. @lab-nmr.bsky.social & @julianvonehr.bsky.social. Check it out:
www.researchsquare.com/article/rs-9...
www.researchsquare.com/article/rs-9...
June 17, 2026 at 1:59 PM
How can a splicing factor bind to thousands of exons but regulate only a few of them? In our new preprint, we show that SRSF6 resolves this paradox through two distinct binding modes. @lab-nmr.bsky.social & @julianvonehr.bsky.social. Check it out:
www.researchsquare.com/article/rs-9...
www.researchsquare.com/article/rs-9...
I’m thrilled to share that my PhD work has been just published in Cell. After a long and bumpy ride, we uncovered the core function of nuclear speckles -splicing of GC-levelled exons- and traced the evolution of this gene architecture and condensates themselves to amniotes.
February 25, 2026 at 4:53 PM
I’m thrilled to share that my PhD work has been just published in Cell. After a long and bumpy ride, we uncovered the core function of nuclear speckles -splicing of GC-levelled exons- and traced the evolution of this gene architecture and condensates themselves to amniotes.
📣 Attention #RNA #Splicing lovers! How is splicing of exons coordinated??🤔 ➡️ Exon Junction Complex (EJC) coordinates inclusion of blocks of neighboring exons!😯 Cool work by Alexandra Bergfort @karlaneugebauer.bsky.social lab - check it out! (we got to help a bit 😊) genesdev.cshlp.org/content/earl...
October 17, 2025 at 2:12 AM
📣 Attention #RNA #Splicing lovers! How is splicing of exons coordinated??🤔 ➡️ Exon Junction Complex (EJC) coordinates inclusion of blocks of neighboring exons!😯 Cool work by Alexandra Bergfort @karlaneugebauer.bsky.social lab - check it out! (we got to help a bit 😊) genesdev.cshlp.org/content/earl...
The #Drosophila Dscam1 gene generates 10000s of isoforms, but only a small fraction supports neuronal functions. This study shows that #fitness & #immunity are the likely primary evolutionary drivers of Dscam1 isoform diversity in #arthropods @plosbiology.org 🧪 plos.io/4gp8cWd
September 15, 2025 at 5:15 PM
The #Drosophila Dscam1 gene generates 10000s of isoforms, but only a small fraction supports neuronal functions. This study shows that #fitness & #immunity are the likely primary evolutionary drivers of Dscam1 isoform diversity in #arthropods @plosbiology.org 🧪 plos.io/4gp8cWd
I'm so excited for each and every Exons update. I cannot shut up about it
More happy slice of my life from my little #queer #dystopian near-future family drama
www.scribblehub.com/series/23835...
www.scribblehub.com/series/23835...
Exons
When Erika got a biotech job at Neuragene she thought she'd got it made. But like most things in life, it turns out it's not as good as it seemed, and the plush job came with a catch she decided she c...
www.scribblehub.com
September 23, 2026 at 7:23 AM
I'm so excited for each and every Exons update. I cannot shut up about it
Check out our new preprint on thousands of novel recursive exons in the human genome (if you don't know about recursive exons, they are pretty cool), led by PhD student David Bass @davidb256.bsky.social : www.biorxiv.org/content/10.6...
Large-scale analysis of transcript data reveals thousands of recursive splicing events in human introns
Recursive splicing (RS) is a process in which an intron is removed from a nascent RNA molecule in two or more splicing events rather than one. We introduce a novel approach for detecting recursive spl...
www.biorxiv.org
September 13, 2026 at 3:40 PM
Check out our new preprint on thousands of novel recursive exons in the human genome (if you don't know about recursive exons, they are pretty cool), led by PhD student David Bass @davidb256.bsky.social : www.biorxiv.org/content/10.6...
1\ Excited to finally share some of my PhD work: OpenSplice!
We measured how >590,000 mutations affect exon inclusion across 600 human exons, creating a resource to study splicing regulation, benchmark variant effect predictors, and support variant interpretation.
We measured how >590,000 mutations affect exon inclusion across 600 human exons, creating a resource to study splicing regulation, benchmark variant effect predictors, and support variant interpretation.
OpenSplice: the impact of half a million mutations on the alternative splicing of 600 human exons https://www.biorxiv.org/content/10.64898/2026.05.22.727141v1
May 26, 2026 at 3:08 PM
1\ Excited to finally share some of my PhD work: OpenSplice!
We measured how >590,000 mutations affect exon inclusion across 600 human exons, creating a resource to study splicing regulation, benchmark variant effect predictors, and support variant interpretation.
We measured how >590,000 mutations affect exon inclusion across 600 human exons, creating a resource to study splicing regulation, benchmark variant effect predictors, and support variant interpretation.
A gene is a segment of a DNA molecule with multiple regions: a promoter, exons (the bits that dictate amino acid sequence in a protein), introns, & terminator regions.
mRNA is a short-lived intermediate of exon segments of DNA — it is a TRANSCRIPT of a piece of the gene.
2/
mRNA is a short-lived intermediate of exon segments of DNA — it is a TRANSCRIPT of a piece of the gene.
2/
March 2, 2025 at 7:51 PM
A gene is a segment of a DNA molecule with multiple regions: a promoter, exons (the bits that dictate amino acid sequence in a protein), introns, & terminator regions.
mRNA is a short-lived intermediate of exon segments of DNA — it is a TRANSCRIPT of a piece of the gene.
2/
mRNA is a short-lived intermediate of exon segments of DNA — it is a TRANSCRIPT of a piece of the gene.
2/
Introducing OpenSplice, an atlas of splice-altering variants and the largest systematic analysis of splicing regulatory variants
OpenSplice quantifies the impact of 590,000+ variants on the splicing of 608 alternatively spliced exons, and is out now on bioRxiv 🧬🖥️
www.biorxiv.org/content/10.6...
OpenSplice quantifies the impact of 590,000+ variants on the splicing of 608 alternatively spliced exons, and is out now on bioRxiv 🧬🖥️
www.biorxiv.org/content/10.6...
May 27, 2026 at 10:25 AM
Introducing OpenSplice, an atlas of splice-altering variants and the largest systematic analysis of splicing regulatory variants
OpenSplice quantifies the impact of 590,000+ variants on the splicing of 608 alternatively spliced exons, and is out now on bioRxiv 🧬🖥️
www.biorxiv.org/content/10.6...
OpenSplice quantifies the impact of 590,000+ variants on the splicing of 608 alternatively spliced exons, and is out now on bioRxiv 🧬🖥️
www.biorxiv.org/content/10.6...
The #Drosophila Dscam1 gene generates 10000s of isoforms, but only a small fraction supports neuronal functions. This study shows that #fitness & #immunity are the likely primary evolutionary drivers of Dscam1 isoform diversity in #arthropods @plosbiology.org 🧪 plos.io/4gp8cWd
September 15, 2025 at 8:44 AM
The #Drosophila Dscam1 gene generates 10000s of isoforms, but only a small fraction supports neuronal functions. This study shows that #fitness & #immunity are the likely primary evolutionary drivers of Dscam1 isoform diversity in #arthropods @plosbiology.org 🧪 plos.io/4gp8cWd
Excited to release #OpenSplice - the impact of >500,000 mutations on the alternative splicing of 600 human exons
www.biorxiv.org/content/10.6...
Led by @gioia-quarantani.bsky.social + funded by @opentargets.org @sangerinstitute.bsky.social @crg.eu
#cshlsymp26 #RNA2026 @rnasociety.bsky.social
www.biorxiv.org/content/10.6...
Led by @gioia-quarantani.bsky.social + funded by @opentargets.org @sangerinstitute.bsky.social @crg.eu
#cshlsymp26 #RNA2026 @rnasociety.bsky.social
www.biorxiv.org
May 28, 2026 at 12:55 PM
Excited to release #OpenSplice - the impact of >500,000 mutations on the alternative splicing of 600 human exons
www.biorxiv.org/content/10.6...
Led by @gioia-quarantani.bsky.social + funded by @opentargets.org @sangerinstitute.bsky.social @crg.eu
#cshlsymp26 #RNA2026 @rnasociety.bsky.social
www.biorxiv.org/content/10.6...
Led by @gioia-quarantani.bsky.social + funded by @opentargets.org @sangerinstitute.bsky.social @crg.eu
#cshlsymp26 #RNA2026 @rnasociety.bsky.social
Only .2% in exons, though.
April 22, 2026 at 5:53 PM
Only .2% in exons, though.
the reason that AI is so popular amongst devs (and execs) is that it allows devs to focus their energies on the exons - the stuff that matters and makes money - and outsource the intron work to something else
May 5, 2026 at 7:07 PM
the reason that AI is so popular amongst devs (and execs) is that it allows devs to focus their energies on the exons - the stuff that matters and makes money - and outsource the intron work to something else
OpenSplice: the impact of half a million mutations on the alternative splicing of 600 human exons
Gioia Quarantani et al.
www.biorxiv.org/content/10.6...
Gioia Quarantani et al.
www.biorxiv.org/content/10.6...
www.biorxiv.org
May 25, 2026 at 2:21 PM
OpenSplice: the impact of half a million mutations on the alternative splicing of 600 human exons
Gioia Quarantani et al.
www.biorxiv.org/content/10.6...
Gioia Quarantani et al.
www.biorxiv.org/content/10.6...
OpenSplice: the impact of half a million mutations on the alternative splicing of 600 human exons https://www.biorxiv.org/content/10.64898/2026.05.22.727141v1
May 24, 2026 at 6:33 AM
OpenSplice: the impact of half a million mutations on the alternative splicing of 600 human exons https://www.biorxiv.org/content/10.64898/2026.05.22.727141v1
Massive experiment to identify sequences that alter alternative splicing for 600(!!!) exons
July 16, 2026 at 7:27 PM
Massive experiment to identify sequences that alter alternative splicing for 600(!!!) exons
Super excited to see our work on hybrid exon evolution out at @narjournal.bsky.social! academic.oup.com/nar/advance-... 1/6
Hybrid exons evolved by coupling transcription initiation and splicing at the nucleotide level
Abstract. Exons within transcripts are traditionally classified as first, internal or last exons, each governed by different regulatory mechanisms. We rece
academic.oup.com
January 16, 2025 at 4:14 PM
Super excited to see our work on hybrid exon evolution out at @narjournal.bsky.social! academic.oup.com/nar/advance-... 1/6