October 18, 2024 at 1:35 PM
Go find new linguidtic changes, compare corpora and invent
huggingface.co/Hplm
arxiv.org/abs/2504.05523
huggingface.co/Hplm
arxiv.org/abs/2504.05523
Hplm (Historical Perspectival LM)
Org profile for Historical Perspectival LM on Hugging Face, the AI community building the future.
huggingface.co
April 15, 2025 at 12:45 PM
Go find new linguidtic changes, compare corpora and invent
huggingface.co/Hplm
arxiv.org/abs/2504.05523
huggingface.co/Hplm
arxiv.org/abs/2504.05523
For ~century, we’ve asked: why do proliferating cells ferment glucose even when O2 is around? I’m thrilled to share our latest work @natmetabolism.nature.com, led by @thebiokimist.bsky.social. By leveraging conditional essentiality in HPLM, we propose a provocative new answer to this classic Q. 🧵
January 16, 2026 at 4:27 PM
For ~century, we’ve asked: why do proliferating cells ferment glucose even when O2 is around? I’m thrilled to share our latest work @natmetabolism.nature.com, led by @thebiokimist.bsky.social. By leveraging conditional essentiality in HPLM, we propose a provocative new answer to this classic Q. 🧵
excited to share our latest work now online
@natmetabolism.nature.com, led by
@kyle-flickinger.bsky.social, where we unravel a mechanistic basis for the conditional essentiality of NADK, one of the many interesting hits from our previously reported CRISPR screening with HPLM rdcu.be/ekpu6
@natmetabolism.nature.com, led by
@kyle-flickinger.bsky.social, where we unravel a mechanistic basis for the conditional essentiality of NADK, one of the many interesting hits from our previously reported CRISPR screening with HPLM rdcu.be/ekpu6
May 2, 2025 at 3:32 PM
excited to share our latest work now online
@natmetabolism.nature.com, led by
@kyle-flickinger.bsky.social, where we unravel a mechanistic basis for the conditional essentiality of NADK, one of the many interesting hits from our previously reported CRISPR screening with HPLM rdcu.be/ekpu6
@natmetabolism.nature.com, led by
@kyle-flickinger.bsky.social, where we unravel a mechanistic basis for the conditional essentiality of NADK, one of the many interesting hits from our previously reported CRISPR screening with HPLM rdcu.be/ekpu6
excited to share a new preprint from our lab! led by the outstanding (& newly minted PhD) @thebiokimist.bsky.social we first uncover a basis for conditional HK2 essentiality in HPLM - leveraging this dependence then leads us to distinct rationale underlying why the Warburg Effect is beneficial!
Hexokinase detachment from mitochondria drives the Warburg effect to support compartmentalized ATP production https://www.biorxiv.org/content/10.1101/2025.02.07.637120v1
February 10, 2025 at 6:40 PM
excited to share a new preprint from our lab! led by the outstanding (& newly minted PhD) @thebiokimist.bsky.social we first uncover a basis for conditional HK2 essentiality in HPLM - leveraging this dependence then leads us to distinct rationale underlying why the Warburg Effect is beneficial!
happy to have collaborated on this v cool study led by Andy & Aaron from the Sean Palecek and Melissa Skala labs! culturing hPSC-CMs in HPLM effectively enhances their maturation vs traditional media ➡️ potential modeling implications for cardiac biology/CVD!
tinyurl.com/3yutyd8c
tinyurl.com/3yutyd8c
Human plasma‐like medium enhances structural and metabolic maturation of human pluripotent stem cell‐derived cardiomyocytes
Human plasma-like medium (HPLM), a synthetic physiologic medium containing over 60 metabolites and small ions at concentrations reflective of adult human plasma, enhances the structural and metabolic....
tinyurl.com
November 21, 2025 at 9:26 PM
happy to have collaborated on this v cool study led by Andy & Aaron from the Sean Palecek and Melissa Skala labs! culturing hPSC-CMs in HPLM effectively enhances their maturation vs traditional media ➡️ potential modeling implications for cardiac biology/CVD!
tinyurl.com/3yutyd8c
tinyurl.com/3yutyd8c
Why champagne? Why not HPLM?! 🫡🫡
December 3, 2024 at 11:50 PM
Why champagne? Why not HPLM?! 🫡🫡
The last study from my postdoc @irim-life.bsky.social is available on BioRxiv!👇
We studied how the metabolic environment in human plasma affected HIV replication in primary CD4 T cells using the recent medium HPLM. Surprisingly, CD4 T cells had lower energy metabolism but higher HIV replication! 🤯
We studied how the metabolic environment in human plasma affected HIV replication in primary CD4 T cells using the recent medium HPLM. Surprisingly, CD4 T cells had lower energy metabolism but higher HIV replication! 🤯
Human plasma metabolic environment favours HIV replication in primary CD4 T lymphocytes https://www.biorxiv.org/content/10.1101/2024.12.10.627699v1
December 12, 2024 at 9:09 AM
The last study from my postdoc @irim-life.bsky.social is available on BioRxiv!👇
We studied how the metabolic environment in human plasma affected HIV replication in primary CD4 T cells using the recent medium HPLM. Surprisingly, CD4 T cells had lower energy metabolism but higher HIV replication! 🤯
We studied how the metabolic environment in human plasma affected HIV replication in primary CD4 T cells using the recent medium HPLM. Surprisingly, CD4 T cells had lower energy metabolism but higher HIV replication! 🤯
‘Traumatize the kids’: ICE accused of arresting parents at school bus stops in Connecticut
www.youtube.com/watch?v=hpLM...
www.youtube.com/watch?v=hpLM...
‘Traumatize the kids’: ICE accused of arresting parents at school bus stops in Connecticut
YouTube video by MS NOW
www.youtube.com
September 3, 2026 at 1:40 AM
‘Traumatize the kids’: ICE accused of arresting parents at school bus stops in Connecticut
www.youtube.com/watch?v=hpLM...
www.youtube.com/watch?v=hpLM...
🚨 pleased to share a new preprint made possible through a fantastic collaboration with the Skala & Palecek labs! can medium composition impact the behavior of hPSC-derived cardiomyocytes? Andy & Aaron co-led efforts to tackle this question w/HPLM! tinyurl.com/ux2arjw8
Human Plasma-Like Medium Promotes Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes
Maturing human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) in vitro is critical for advancing drug discovery and cardiotoxicity screening applications of these cells. However, the metaboli...
tinyurl.com
April 28, 2025 at 4:02 PM
🚨 pleased to share a new preprint made possible through a fantastic collaboration with the Skala & Palecek labs! can medium composition impact the behavior of hPSC-derived cardiomyocytes? Andy & Aaron co-led efforts to tackle this question w/HPLM! tinyurl.com/ux2arjw8
This could not be better. youtube.com/watch?v=hpLm...
STEPHEN COLBERT—"ONLY IN MONROE" (MAY 22, 2026 FULL SHOW)
YouTube video by Play Group Media
youtube.com
May 25, 2026 at 1:53 AM
This could not be better. youtube.com/watch?v=hpLm...
Nitya4Eternity #KingsTaster #HPLM #FreeIran ❤️ 💙
@nityalakshmi108
19 Jul 2022
We have issues w/ racial prejudice in Sandiego courts.
FROM BEGINNING OF DIVORCE, '16 COPS STALKED & interrogated about
@nityalakshmi108
19 Jul 2022
We have issues w/ racial prejudice in Sandiego courts.
FROM BEGINNING OF DIVORCE, '16 COPS STALKED & interrogated about
December 30, 2025 at 8:13 AM
Nitya4Eternity #KingsTaster #HPLM #FreeIran ❤️ 💙
@nityalakshmi108
19 Jul 2022
We have issues w/ racial prejudice in Sandiego courts.
FROM BEGINNING OF DIVORCE, '16 COPS STALKED & interrogated about
@nityalakshmi108
19 Jul 2022
We have issues w/ racial prejudice in Sandiego courts.
FROM BEGINNING OF DIVORCE, '16 COPS STALKED & interrogated about
Hey, #mycosky #immunosky #microsky 👋🏻
Earlier in the year, we published a paper where we found that HPLM (human plasma-like media) induces Cryptococcus neoformans growth that closely represents those morphologies found in vivo 🧫🦠
doi.org/10.1128/msph...
Earlier in the year, we published a paper where we found that HPLM (human plasma-like media) induces Cryptococcus neoformans growth that closely represents those morphologies found in vivo 🧫🦠
doi.org/10.1128/msph...
Human plasma-like medium (HPLM) induces Cryptococcus neoformans in vivo cell morphologies | mSphere
We provide a description of new in vitro culture condition using the human plasma-like medium that supports the formation
of the full range of in vivo cell morphologies of C. neoformans.
doi.org
December 4, 2024 at 10:29 AM
Hey, #mycosky #immunosky #microsky 👋🏻
Earlier in the year, we published a paper where we found that HPLM (human plasma-like media) induces Cryptococcus neoformans growth that closely represents those morphologies found in vivo 🧫🦠
doi.org/10.1128/msph...
Earlier in the year, we published a paper where we found that HPLM (human plasma-like media) induces Cryptococcus neoformans growth that closely represents those morphologies found in vivo 🧫🦠
doi.org/10.1128/msph...
ICE’s latest traumatizing tactic in Connecticut: letting kids get on schoolbuses in the morning, then arresting their parents, who mostly have no charges against them. When the kids return from school on the bus, their parents are gone with no explanation.
www.youtube.com/watch?v=hpLM...
www.youtube.com/watch?v=hpLM...
‘Traumatize the kids’: ICE accused of arresting parents at school bus stops in Connecticut
YouTube video by MS NOW
www.youtube.com
September 3, 2026 at 9:53 PM
ICE’s latest traumatizing tactic in Connecticut: letting kids get on schoolbuses in the morning, then arresting their parents, who mostly have no charges against them. When the kids return from school on the bus, their parents are gone with no explanation.
www.youtube.com/watch?v=hpLM...
www.youtube.com/watch?v=hpLM...
Using CRISPR screens, we found that core UFMylation machinery is far more essential in RPMI than in HPLM. While recent landmark studies link UFMylation to the resolution of ribosome stalls at the ER, its broader functional implications have remained elusive. 1/n
March 30, 2026 at 4:14 PM
Using CRISPR screens, we found that core UFMylation machinery is far more essential in RPMI than in HPLM. While recent landmark studies link UFMylation to the resolution of ribosome stalls at the ER, its broader functional implications have remained elusive. 1/n
MS NOW- 9/2
👹☣️💀🤥8647🤥💀☣️👹
#AbolishICE
#DefundICE
#FUCKICE
ICE GESTAPO arrested PARENTS at SCHOOL BUS STOP in DANBURY, CONNECTICUT as its IMMIGRATION CRACKDOWN CONTINUES across the STATE. SENATOR CHRIS MURPHY joins to discuss.
youtube.com/watch?v=hpLM...
👹☣️💀🤥8647🤥💀☣️👹
#AbolishICE
#DefundICE
#FUCKICE
ICE GESTAPO arrested PARENTS at SCHOOL BUS STOP in DANBURY, CONNECTICUT as its IMMIGRATION CRACKDOWN CONTINUES across the STATE. SENATOR CHRIS MURPHY joins to discuss.
youtube.com/watch?v=hpLM...
‘Traumatize the kids’: ICE accused of arresting parents at school bus stops in Connecticut
YouTube video by MS NOW
youtube.com
September 3, 2026 at 4:39 AM
MS NOW- 9/2
👹☣️💀🤥8647🤥💀☣️👹
#AbolishICE
#DefundICE
#FUCKICE
ICE GESTAPO arrested PARENTS at SCHOOL BUS STOP in DANBURY, CONNECTICUT as its IMMIGRATION CRACKDOWN CONTINUES across the STATE. SENATOR CHRIS MURPHY joins to discuss.
youtube.com/watch?v=hpLM...
👹☣️💀🤥8647🤥💀☣️👹
#AbolishICE
#DefundICE
#FUCKICE
ICE GESTAPO arrested PARENTS at SCHOOL BUS STOP in DANBURY, CONNECTICUT as its IMMIGRATION CRACKDOWN CONTINUES across the STATE. SENATOR CHRIS MURPHY joins to discuss.
youtube.com/watch?v=hpLM...
Thank you for the detailed response Jason, that all sounds logical to me! Looking forward to more HPLM discoveries!
May 4, 2025 at 9:37 AM
Thank you for the detailed response Jason, that all sounds logical to me! Looking forward to more HPLM discoveries!
3/11 🛠️ The platform: We used Human Plasma-like Medium (HPLM, shoutout @jasonrcantor.bsky.social) to generate a library covering amino acids, the urea cycle, nucleic acids, ions, and end products to generate system-level portrait of cellular nitrogen substrate preferences.
April 29, 2026 at 10:46 AM
3/11 🛠️ The platform: We used Human Plasma-like Medium (HPLM, shoutout @jasonrcantor.bsky.social) to generate a library covering amino acids, the urea cycle, nucleic acids, ions, and end products to generate system-level portrait of cellular nitrogen substrate preferences.
Human Plasma-Like Medium (HPLM) induces Cryptococcus neoformans in vivo cell morphologies https://www.biorxiv.org/content/10.1101/2023.08.29.555343v1
Human Plasma-Like Medium (HPLM) induces Cryptococcus neoformans in vivo cell morphologies https://www.biorxiv.org/content/10.1101/2023.08.29.555343v1
The fungal pathogen Cryptococcus neoformans (C. neoformans) forms yeast cells of different sizes and
www.biorxiv.org
August 31, 2023 at 3:17 AM
Human Plasma-Like Medium (HPLM) induces Cryptococcus neoformans in vivo cell morphologies https://www.biorxiv.org/content/10.1101/2023.08.29.555343v1
This show has everything. Shots and balloons. Steve Buscemi. A sandwich w Jeff Daniels *and* a dumpster fire. youtube.com/watch?v=hpLm...
STEPHEN COLBERT—"ONLY IN MONROE" (MAY 22, 2026 FULL SHOW)
YouTube video by Play Group Media
youtube.com
May 25, 2026 at 8:13 PM
This show has everything. Shots and balloons. Steve Buscemi. A sandwich w Jeff Daniels *and* a dumpster fire. youtube.com/watch?v=hpLm...
While alanine is the second most abundant amino acid in human blood (provided in HPLM), it is completely absent from RPMI and most classic synthetic media. Our findings indicate that the ER-resident UFMylation system functions as a ‘ribosome collision counter’. 3/n
March 30, 2026 at 4:14 PM
While alanine is the second most abundant amino acid in human blood (provided in HPLM), it is completely absent from RPMI and most classic synthetic media. Our findings indicate that the ER-resident UFMylation system functions as a ‘ribosome collision counter’. 3/n