#nucleosome
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voslab.org
🧪🧬New preprint We present cryo-EM structures of reconstituted CTCF–nucleosome complexes, showing CTCF dimerization drives nucleosome oligomerization into defined higher-order assemblies. Disrupting CTCF–CTCF interfaces in mESCs reduces looping and impairs differentiation. tinyurl.com/CTCF-nucleos...
February 9, 2026 at 12:54 PM
nucleosomepolice.bsky.social
Take that @biologicmodels.bsky.social. Welded #nucleosome, created by the insanely talented @bryanwelm.bsky.social
December 18, 2024 at 1:51 PM
lucas.farnunglab.com
In our IWS1 story, we also visualize the histone reader LEDGF PWWP domain on a transcribed downstream nucleosome. Because the PWWP requires both DNA gyres for binding, it binds to the promoter-distal side of the nucleosome during transcription through the nucleosome.
LEDGF PWWP (pink) binds the transcribed downstream nucleosome. A cryo-EM map (grey) verifies the positioning of the activated transcription elongation complex in relation to the downstream nucleosome.
September 3, 2025 at 3:42 PM
alexis-verger.cpesr.fr
Well, somebody had to do it @nucleosomepolice.bsky.social 😬

Did you know that the nucleosome inspires the Millennium Falcon design? #Maythe4thBeWithYou #MayThe4th #MayTheFourthBeWithYou
May 4, 2025 at 6:30 AM
alexis-verger.cpesr.fr
Did you know that Pierre Chambon's laboratory was the first to use the term "nucleosome" in 1975 ?

www.cell.com/cell/fulltex...
May 8, 2026 at 10:27 AM
benoitbruneau.bsky.social
But wait! There’s more! So you thought you knew nucleosome structure? Think again! @vram142.bsky.social @gladstoneinst.bsky.social and @genophoria.bsky.social @arcinstitute.org show that there is plenty of distortion largely due to TFs butting in www.nature.com/articles/s41...
Pervasive and programmed nucleosome distortion on single chromatin fibres - Nature
An analytical pipeline called Iteratively Defined Lengths of Inaccessibility (IDLI) maps the genome-wide occupancy of a range of nucleosome types and shows that most nucleosomes exhibit programmed ‘di...
www.nature.com
April 30, 2026 at 8:50 PM
alexis-verger.cpesr.fr
#RIP Pierre Chambon (1931-2026) via @alainberetz.bsky.social

one of the last giants in biology: PARP, nuclear receptors, RNA Pol II, nucleosome, promoters, split genes
May 7, 2026 at 10:00 AM
biorxiv-biochem.bsky.social
Reconstitution of +1 nucleosome transcription reveals coordinated functions of SAGA, Mediator, and TFIIH https://www.biorxiv.org/content/10.64898/2026.09.24.754195v1
September 28, 2026 at 10:46 AM
alexis-verger.cpesr.fr
#RIP Pierre Chambon (1931-2026)

1963 PARP
1970 RNA Pol II
1975 Nucleosome
1977 Split genes
1980 Promoter
1983 enhancer
1986 Nuclear receptor
May 7, 2026 at 10:39 AM
teiflab.bsky.social
Woolfe et al, 2026. Decoding the mystery of ultra-conservation in developmental enhancers: a role for nucleosome positioning, DNA structure and transcription factor binding www.biorxiv.org/content/10.6...
▶️conserved non-coding elements ... favor nucleosome occupancy at their borders
July 26, 2026 at 9:34 AM
andersshansen.bsky.social
Very nice new paper from Paggi, Long, Zhang using physical modeling of RCMC data to reconstruct physical structures of euchromatin at the nucleosome scale:

Euchromatin forms condensed domains and E-P interactions largely occur on the surface!
www.nature.com/articles/s41...
September 28, 2026 at 1:49 PM
teiflab.bsky.social
Nucleosome aficionados! Our new review "Nucleosome spacing across cell types, diseases, and ages" is out in NAR: academic.oup.com/nar/article/...

A huge effort to pull together what we’ve learned about nucleosome spacing in many systems. Enjoy!
@milena-bikova.bsky.social @chrsclrksn.bsky.social
Figure 1.(A) Classical gel electrophoresis experiments showing mono-, di-, tri-, tetra-, and further multinucleosome bands upon chromatin digestion. (B) The nucleosome repeat length (NRL) is defined as the genomic distance between the centres of two neighbouring nucleosomes. Figure 2.Nucleosome mapping using MNase-seq versus ATAC-seq. (A) In MNase-seq, nucleosomes in both open and tightly packed genomic regions are accessible to digestion. MNase preferentially cleaves DNA between nucleosomes and digests DNA until it encounters a histone octamer, which provides a footprint of nucleosome-protected DNA regions. (B) Bulk MNase-seq results in averaged maps across millions of cells, effectively capturing all possible nucleosome positioning configurations. (C) Single-cell MNase-seq (scMNase-seq) results in a noisier and sparser signal. The resulting footprints still represent nucleosome-protected regions, but not all nucleosomes are represented. (D) In ATAC-seq, open regions can be accessed by the enzyme Tn5 transposase, which can insert primers in regions free from the binding of nucleosomes and transcription factors (TFs). (E) For open chromatin regions, nucleosome maps can be obtained from ATAC-seq similar to MNase-seq. (F) Closed, tightly packed chromatin regions may be less represented in ATAC-seq nucleosome maps. Figure 5.Molecular mechanisms affecting nucleosome spacing. (A) Linker histones H1 and nonhistone chromatin proteins which compete with H1s and modulate nucleosome spacing through structural and electrostatic mechanisms. (B) Chromatin remodellers actively reposition nucleosomes following context-dependent rules. (C) Cell state-dependent chromatin boundaries formed by CTCF and other structural proteins, as well as associated recruitment of chromatin remodellers which space nucleosomes. (D) Gene activity associated with remodeller action and RNA polymerases transcribing through the nucleosomes, leading to smaller distances between nucleosomes in regulatory regions and gene bodies. (E) DNA sequence repeats of different types. Figure 6. Examples of NRL changes in biological systems. (A) Cell differentiation leads to NRL changes between different cell types, e.g. mouse dorsal root ganglia neurons (NRL ∼165 bp) versus cortical astrocytes (NRL ∼183 bp) [175]. Schematic cell shapes are adapted from an image created in BioRender (https://BioRender.com/89trj2t). (B) Paired normal versus tumour breast tissues show NRL shortening in cancer (figure adapted from [36] under the CC BY 4.0 licence (https://creativecommons.org/licenses/by/4.0/)). (C) Nucleosome positioning derived from cfDNA of human volunteers shows NRL increase with age (figure reprinted from [79] under the CC BY 4.0 licence (https://creativecommons.org/licenses/by/4.0/)).
March 5, 2026 at 9:32 PM
hainerlab.bsky.social
Our collaborative paper with the Arndt lab is out today: doi.org/10.1093/nar/... (originally bioRxiv: www.biorxiv.org/content/10.1...). Here we asked the question of how CHD1 localizes to chromatin.
A direct interaction between the Chd1 CHCT domain and Rtf1 controls Chd1 distribution and nucleosome positioning on active genes
Abstract. The nucleosome remodeler Chd1 is required for the re-establishment of nucleosome positioning in the wake of transcription elongation by RNA Polym
doi.org
August 28, 2025 at 11:07 PM
nucleosomepolice.bsky.social
So, here I am. The nucleosome police now flies under their official tag. Please follow me if you are interested in #chromatin, #nucleosome, #cryoEM, #scienceeducation, #hiking, #quilting, and #nature. Help me figure out this platform!
September 23, 2023 at 3:55 PM
joyjoychoi.bsky.social
Your nucleosome is electric! ⚡
September 27, 2026 at 10:39 AM
vram142.bsky.social
Delighted to share our lab's latest (w/ inimitable @genophoria.bsky.social) in final form at @nature.com. Enormous lift by Sean Wang, @palindromephd.bsky.social & @martyyang.bsky.social to address extensive & constructive reviewer comments & see this through. (1/n) www.nature.com/articles/s41...
Pervasive and programmed nucleosome distortion on single chromatin fibres - Nature
An analytical pipeline called Iteratively Defined Lengths of Inaccessibility (IDLI) maps the genome-wide occupancy of a range of nucleosome types and shows that most nucleosomes exhibit programmed ‘di...
www.nature.com
May 6, 2026 at 2:32 PM
qsyami.eurosky.social
BTS as histones in a nucleosome. BTS as a dance your PhD challenge.
qsyami.eurosky.social soch @qsyami.eurosky.social · Nov 11
The #BTS DNA choreography involves some deep biology where they dance a nucleosome. It's been shown that DNA (arms) tighten, loosen, and twist around histones (the guy's bodies) in cells which serves a packaging role and also affects which portions of the DNA can be easily read.

#DNA #nucleosomes
On the left, the scene from the DNA music video at 3:07 where the choreography forms a knot. On the right, a diagram of a DNA nucleosome.
July 30, 2025 at 2:01 PM
lucas.farnunglab.com
Our paper on the structure of the complete Rpd3S-nucleosome complex is now out in Nat Comm: rdcu.be/ds6k1 . Congrats to @jonmarkert.bsky.social
and co-corresponding author Seychelle Vos! This is the second HDAC-nucleosome structure from Jon this year. Amazing!
Structure of Rpd3S-nucleosome complex.
December 8, 2023 at 12:32 PM
nucleosomepolice.bsky.social
@nucleosomepolice.bsky.social approves wholeheartedly. More #nucleosome #scienceart
irinabezsonova.bsky.social Irina Bezsonova @irinabezsonova.bsky.social · Nov 2
This October I’m drawing 1 molecule a day inspired by proteins in pdb @rcsbpdb.bsky.social

Day 20/31
Prompt RIVALS
Pdb: 8V27 & 8G6S

Two rival histone modifications on a nucleosome. Ubiquitination of H2BK120 activates genes while H2AK119 represses them.

Next: BLAST
Suggestions?
November 4, 2025 at 5:41 PM
lucas.farnunglab.com
🚨Preprint alert🚨 How does chromatin “architecture” form at CTCF sites? Our new preprint with @voslab.org and @andersshansen.bsky.social shows CTCF dimerization promotes nucleosome oligomerization on chromatin. tinyurl.com/CTCF-nucleos...
February 9, 2026 at 3:04 PM
teiflab.bsky.social
Nucleosome fibre topology guides transcription factor binding to enhancers www.nature.com/articles/s41...
▶️motif recognition on mononucleosomes affects only an individual TF binding
▶️When bound together, TFs act cooperatively or competitively to target nucleosome arrays with defined 3D organization
December 18, 2024 at 6:53 PM
eltsovmikhail.bsky.social
We are in EMBO J. See DNA and non-octameric nucleosome-like particles in situ

link.springer.com/article/10.1...
Direct visualization and tracing of chromatin folding in the Drosophila embryo - The EMBO Journal
Chromatin organization, through the assembly of DNA with histones and the folding of nucleosome chains, regulates DNA accessibility for transcription, DNA replication and repair. Although models deriv...
link.springer.com
February 10, 2026 at 3:44 PM
kazu-maeshima.bsky.social
Our new preprint! 🧬✨ www.biorxiv.org/cgi/content/...
@ynagata.bsky.social et al. combined single-nucleosome imaging with Fucci probes to follow local chromatin behavior during interphase. Local nucleosome motion stays nearly constant from G1 to G2, except in early G1. Thanks to all coauthors!
May 1, 2026 at 4:24 PM
kazu-maeshima.bsky.social
My Fragile Nucleosome seminar is now on YouTube
www.youtube.com/watch?v=ksXp...
I discuss our recent work showing that linker histone H1 behaves as a liquid-like glue for chromatin organization in living human cells 🧬
Paper:
www.science.org/doi/full/10....
Huge thanks to @fnucleosome.bsky.social!
Kazuhiro Maeshima
YouTube video by Fragile Nucleosome
www.youtube.com
June 3, 2026 at 2:22 AM
teiflab.bsky.social
Nucleosome spacing can fine-tune higher order chromatin assembly [labs of Rosana Collepardo-Guevara and Michael Rosen]
www.biorxiv.org/content/10.1...
▶️phase separation is decided by trade-off between inter- and intramolecular nucleosome stacking, favoured by 10N+5 and 10N bp linkers, respectively
December 28, 2024 at 8:13 PM