New work from the lab! We report that DDIAS is a downstream effector of CIP2A-TOPBP1 in mitosis that suppresses ssDNA to preserve genome integrity. From the great @yiboxue.bsky.social and many collaborators!
Free link: authors.elsevier.com/a/1nfiF3vVUP...
Pay link: www.cell.com/molecular-ce...
Free link: authors.elsevier.com/a/1nfiF3vVUP...
Pay link: www.cell.com/molecular-ce...
August 24, 2026 at 11:37 PM
New work from the lab! We report that DDIAS is a downstream effector of CIP2A-TOPBP1 in mitosis that suppresses ssDNA to preserve genome integrity. From the great @yiboxue.bsky.social and many collaborators!
Free link: authors.elsevier.com/a/1nfiF3vVUP...
Pay link: www.cell.com/molecular-ce...
Free link: authors.elsevier.com/a/1nfiF3vVUP...
Pay link: www.cell.com/molecular-ce...
Well, this preprint is not about bat shit, but I believe the term "bat shit" should be used as part of a descriptor of this preprint:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Discovery of single-stranded DNA in meteorite-derived cultures: evidence of novel genetic elements
This study presents the first evidence of single-stranded DNA (ssDNA) sequences identified through shotgun metagenomic analysis of meteorite-derived cultures. These ssDNA sequences, predominantly isol...
www.biorxiv.org
January 26, 2025 at 6:23 AM
Well, this preprint is not about bat shit, but I believe the term "bat shit" should be used as part of a descriptor of this preprint:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The DdmDE defense system eradicates plasmids by target-centered bidirectional ssDNA loop extrusion and site-specific cleavage
The DdmDE defense system eradicates plasmids by target-centered bidirectional ssDNA loop extrusion and site-specific cleavage
Yang et al. reveal a dynamic DdmDE-mediated plasmid clearance pathway. Transient DNA bubbles permit promiscuous DdmE binding, while target specificity arises from slower dissociation at matched sites. DdmE then recruits a DdmD dimer to drive target-centered, bidirectional dsDNA unwinding. Free DdmD subsequently coats extruded ssDNA to enable site-specific endonucleolytic cleavage.
dlvr.it
June 19, 2026 at 3:20 PM
The DdmDE defense system eradicates plasmids by target-centered bidirectional ssDNA loop extrusion and site-specific cleavage
1/5
Happy by a recent biorxiv pre-print about DARNA (PD-T7-3) anti-phage defense system. This system is activated by binding to ssDNA, presented by phage SSB, to cleave tRNA in the anti-codon loop.
www.biorxiv.org/content/10.6...
Happy by a recent biorxiv pre-print about DARNA (PD-T7-3) anti-phage defense system. This system is activated by binding to ssDNA, presented by phage SSB, to cleave tRNA in the anti-codon loop.
www.biorxiv.org/content/10.6...
February 24, 2026 at 8:08 AM
1/5
Happy by a recent biorxiv pre-print about DARNA (PD-T7-3) anti-phage defense system. This system is activated by binding to ssDNA, presented by phage SSB, to cleave tRNA in the anti-codon loop.
www.biorxiv.org/content/10.6...
Happy by a recent biorxiv pre-print about DARNA (PD-T7-3) anti-phage defense system. This system is activated by binding to ssDNA, presented by phage SSB, to cleave tRNA in the anti-codon loop.
www.biorxiv.org/content/10.6...
New preprint from the lab for any DNA repair or replication stress geeks out there!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
A nuclease-driven mechanism of post-replicative ssDNA gap suppression.
The persistence of post-replicative ssDNA gaps following PRIMPOL-mediated replication repriming is linked to chemosensitivity, and in all models reported to date the nuclease MRE11 has been implicated...
www.biorxiv.org
October 22, 2025 at 6:56 PM
New preprint from the lab for any DNA repair or replication stress geeks out there!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Our last article about ssDNA gaps and BRCA1 👇🏻👇🏻
doi.org/10.1093/nar/...
doi.org/10.1093/nar/...
EXO1 and DNA2-mediated ssDNA gap expansion is essential for ATR activation and to maintain viability in BRCA1-deficient cells
Abstract. DNA replication faces challenges from DNA lesions originated from endogenous or exogenous sources of stress, leading to the accumulation of singl
doi.org
December 6, 2024 at 11:06 PM
Our last article about ssDNA gaps and BRCA1 👇🏻👇🏻
doi.org/10.1093/nar/...
doi.org/10.1093/nar/...
Ever wondered how TIMELESS/TIPIN can sit at the leading edge of the replication fork regulating its speed, but also bind polymerases and ssDNA/RPA in replication stress? We have an idea how it might work! Check out our new preprint, any feedback is highly appreciated!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
December 18, 2025 at 3:04 PM
Ever wondered how TIMELESS/TIPIN can sit at the leading edge of the replication fork regulating its speed, but also bind polymerases and ssDNA/RPA in replication stress? We have an idea how it might work! Check out our new preprint, any feedback is highly appreciated!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Check out our new paper led by @seppai99.bsky.social from the Vindigni Lab in collaboration with @cejkalab.bsky.social delving into the role of nucleases resecting ssDNA gaps generated by PARPi. You can read more here: genesdev.cshlp.org/content/earl...
March 26, 2025 at 5:20 PM
Check out our new paper led by @seppai99.bsky.social from the Vindigni Lab in collaboration with @cejkalab.bsky.social delving into the role of nucleases resecting ssDNA gaps generated by PARPi. You can read more here: genesdev.cshlp.org/content/earl...
If you are interested in ssDNA viruses of bacteria, archaea or eukaryotes (yes, from all walks of life!), this is the conference for you - International Symposium on ssDNA Viruses (ISS3DV)! isdv2026.com
The deadline for the early bird registration is approaching...
The deadline for the early bird registration is approaching...
January 16, 2026 at 4:53 PM
If you are interested in ssDNA viruses of bacteria, archaea or eukaryotes (yes, from all walks of life!), this is the conference for you - International Symposium on ssDNA Viruses (ISS3DV)! isdv2026.com
The deadline for the early bird registration is approaching...
The deadline for the early bird registration is approaching...
What an amazing #IS3DV conference (isdv2026.com) in Ilhéus, Brazil! A big thanks to Murilo Zerbini&team for the fantastic organization — we’re looking forward to IS3DV 2029 in Málaga! (ssDNA virus enthusiasts, stay tuned!) @lauramepul.bsky.social @huangtan.bsky.social
June 21, 2026 at 3:40 PM
What an amazing #IS3DV conference (isdv2026.com) in Ilhéus, Brazil! A big thanks to Murilo Zerbini&team for the fantastic organization — we’re looking forward to IS3DV 2029 in Málaga! (ssDNA virus enthusiasts, stay tuned!) @lauramepul.bsky.social @huangtan.bsky.social
New paper published in @natcomms.nature.com!
Population ecology and biogeochemical implications of ssDNA and dsDNA viruses along a permafrost thaw gradient
doi.org/10.1038/s414...
www.nature.com/collections/...
@sullivan-lab.bsky.social
Population ecology and biogeochemical implications of ssDNA and dsDNA viruses along a permafrost thaw gradient
doi.org/10.1038/s414...
www.nature.com/collections/...
@sullivan-lab.bsky.social
Population ecology and biogeochemical implications of ssDNA and dsDNA viruses along a permafrost thaw gradient - Nature Communications
The potential roles of viruses in microbial-induced permafrost thaw, which is accelerated by climate change, are unclear. Here, Trubl et al. sample a permafrost thaw gradient and identify thousands of...
doi.org
January 16, 2026 at 6:16 PM
New paper published in @natcomms.nature.com!
Population ecology and biogeochemical implications of ssDNA and dsDNA viruses along a permafrost thaw gradient
doi.org/10.1038/s414...
www.nature.com/collections/...
@sullivan-lab.bsky.social
Population ecology and biogeochemical implications of ssDNA and dsDNA viruses along a permafrost thaw gradient
doi.org/10.1038/s414...
www.nature.com/collections/...
@sullivan-lab.bsky.social
what the fuck www.biorxiv.org/content/10.1...
Discovery of single-stranded DNA in meteorite-derived cultures: evidence of novel genetic elements
This study presents the first evidence of single-stranded DNA (ssDNA) sequences identified through shotgun metagenomic analysis of meteorite-derived cultures. These ssDNA sequences, predominantly isol...
www.biorxiv.org
January 26, 2025 at 6:43 AM
what the fuck www.biorxiv.org/content/10.1...
2/3 Mondal & Thirumalai (2026) https://arxiv.org/pdf/2608.25046v1 show that polyvalent cations increase ssDNA persistence length via electrostatic screening and stacking, turning a floppy polymer into a semi‑rigid filament. #academicsky #biophysics #booksky
September 23, 2026 at 8:00 PM
2/3 Mondal & Thirumalai (2026) https://arxiv.org/pdf/2608.25046v1 show that polyvalent cations increase ssDNA persistence length via electrostatic screening and stacking, turning a floppy polymer into a semi‑rigid filament. #academicsky #biophysics #booksky
2) The histone chaperone CAF1 promotes PRIMPOL recruitment to restart stalled forks through ssDNA gap formation. doi.org/10.1093/nar/...
CAF-1 promotes efficient PrimPol recruitment to nascent DNA for single-stranded DNA gap formation
Abstract. Suppression of single-stranded DNA (ssDNA) gap accumulation at replication forks has emerged as a potential determinant of chemosensitivity in ho
doi.org
December 18, 2024 at 3:45 PM
2) The histone chaperone CAF1 promotes PRIMPOL recruitment to restart stalled forks through ssDNA gap formation. doi.org/10.1093/nar/...
Indeed, ssDNA is taken up during natural competence, see Dave Dubnau papers on Bacillus subtilis, summarized here: www.science.org/doi/10.1126/...
December 29, 2024 at 9:24 PM
Indeed, ssDNA is taken up during natural competence, see Dave Dubnau papers on Bacillus subtilis, summarized here: www.science.org/doi/10.1126/...
Hello, Bluesky!
To kick things off here, we’re excited to share: The SLFN11 story continues! We combined cryo-EM and biochemical assays to explain how phosphorylation regulates SLFN11´s tRNA cleavage and ssDNA binding activities.
Check out our new paper here www.nature.com/articles/s41... + movie
To kick things off here, we’re excited to share: The SLFN11 story continues! We combined cryo-EM and biochemical assays to explain how phosphorylation regulates SLFN11´s tRNA cleavage and ssDNA binding activities.
Check out our new paper here www.nature.com/articles/s41... + movie
December 3, 2024 at 1:00 PM
Hello, Bluesky!
To kick things off here, we’re excited to share: The SLFN11 story continues! We combined cryo-EM and biochemical assays to explain how phosphorylation regulates SLFN11´s tRNA cleavage and ssDNA binding activities.
Check out our new paper here www.nature.com/articles/s41... + movie
To kick things off here, we’re excited to share: The SLFN11 story continues! We combined cryo-EM and biochemical assays to explain how phosphorylation regulates SLFN11´s tRNA cleavage and ssDNA binding activities.
Check out our new paper here www.nature.com/articles/s41... + movie
Interesting... single author ob/gyn doc... 🧬🖥️🧪👽🛸
Discovery of single-stranded DNA in meteorite-derived cultures: evidence of novel genetic elements
Discovery of single-stranded DNA in meteorite-derived cultures: evidence of novel genetic elements
Discovery of single-stranded DNA in meteorite-derived cultures: evidence of novel genetic elements
This study presents the first evidence of single-stranded DNA (ssDNA) sequences identified through shotgun metagenomic analysis of meteorite-derived cultures. These ssDNA sequences, predominantly…
www.biorxiv.org
January 26, 2025 at 11:22 AM
Interesting... single author ob/gyn doc... 🧬🖥️🧪👽🛸
Discovery of single-stranded DNA in meteorite-derived cultures: evidence of novel genetic elements
Discovery of single-stranded DNA in meteorite-derived cultures: evidence of novel genetic elements
Viral SSB-bound ssDNA activates the bacterial anti-phage defense system DARNA https://www.biorxiv.org/content/10.64898/2026.02.22.705581v1
February 24, 2026 at 2:17 AM
Viral SSB-bound ssDNA activates the bacterial anti-phage defense system DARNA https://www.biorxiv.org/content/10.64898/2026.02.22.705581v1
Paired-Damage-seq is an in situ labeling-by-repair strategy for joint profiling of oxidative and ssDNA damage alongside gene expression, in single cells.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
March 28, 2025 at 5:14 PM
Paired-Damage-seq is an in situ labeling-by-repair strategy for joint profiling of oxidative and ssDNA damage alongside gene expression, in single cells.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Designed ssDNA traps relocate proteins to disable their function and block tumor growth
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
January 2, 2026 at 12:58 AM
Designed ssDNA traps relocate proteins to disable their function and block tumor growth
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Fantastic work by @GoldlustKelly and the @tacc_c Lesterlin lab demonstrating distant conjugative transfer of ssDNA through the F pilus. 👏 #MicroSky www.pnas.org/doi/10.1073/...
November 15, 2023 at 9:25 AM
Fantastic work by @GoldlustKelly and the @tacc_c Lesterlin lab demonstrating distant conjugative transfer of ssDNA through the F pilus. 👏 #MicroSky www.pnas.org/doi/10.1073/...
www.nature.com/articles/s41...
Pretty amazing.
The extracellular injection system (what I call: "T6SS understudied cousin") can inject both proteins and ssDNA into target cells. Quite useful for biotech applications.
Another great job from Joe Krietz from Feng Zhang's lab.
Pretty amazing.
The extracellular injection system (what I call: "T6SS understudied cousin") can inject both proteins and ssDNA into target cells. Quite useful for biotech applications.
Another great job from Joe Krietz from Feng Zhang's lab.
Targeted delivery of diverse biomolecules with engineered bacterial nanosyringes - Nature Biotechnology
Ribonucleoproteins, nucleic acids and proteins are delivered into target cell types with nanosyringes.
www.nature.com
August 13, 2025 at 5:53 PM
www.nature.com/articles/s41...
Pretty amazing.
The extracellular injection system (what I call: "T6SS understudied cousin") can inject both proteins and ssDNA into target cells. Quite useful for biotech applications.
Another great job from Joe Krietz from Feng Zhang's lab.
Pretty amazing.
The extracellular injection system (what I call: "T6SS understudied cousin") can inject both proteins and ssDNA into target cells. Quite useful for biotech applications.
Another great job from Joe Krietz from Feng Zhang's lab.
Very pleased to share our new story on SMC complexes, a great team effort from @roisnehamelinf.bsky.social, @taschner.bsky.social in the GruberLab and James of @dnatopologylab.bsky.social We find that SMC hinge domains are ssDNA gates to facilitate obstacle bypass during DNA loop extrusion!
March 18, 2025 at 8:03 AM
Very pleased to share our new story on SMC complexes, a great team effort from @roisnehamelinf.bsky.social, @taschner.bsky.social in the GruberLab and James of @dnatopologylab.bsky.social We find that SMC hinge domains are ssDNA gates to facilitate obstacle bypass during DNA loop extrusion!
Really excited to share my co-first author paper with @cejkalab.bsky.social looking at how/which nucleases are involved in resecting ssDNA gaps generated by PARPi!
Can’t wait to talk about our work at the DNA Replication Gaps, Cancer and Disease meeting in South Korea next month!
Can’t wait to talk about our work at the DNA Replication Gaps, Cancer and Disease meeting in South Korea next month!
Check out our new paper led by @seppai99.bsky.social from the Vindigni Lab in collaboration with @cejkalab.bsky.social delving into the role of nucleases resecting ssDNA gaps generated by PARPi. You can read more here: genesdev.cshlp.org/content/earl...
March 26, 2025 at 5:49 PM
Really excited to share my co-first author paper with @cejkalab.bsky.social looking at how/which nucleases are involved in resecting ssDNA gaps generated by PARPi!
Can’t wait to talk about our work at the DNA Replication Gaps, Cancer and Disease meeting in South Korea next month!
Can’t wait to talk about our work at the DNA Replication Gaps, Cancer and Disease meeting in South Korea next month!