Very happy to share that our work (beautifully led by @miemonti.bsky.social and #Hasan) is finally out! 😄We find that tRNAs engaged in actively translating ribosomes significantly differ from the 'total' pool available in the cell, specially upon stress! www.nature.com/articles/s41...
Selective profiling of translationally active tRNAs and their dynamics under stress - Nature Methods
tRIBO-seq distinguishes between ribosome-associated tRNAs and total tRNAs using nanopore sequencing, thus revealing their potentially distinct abundance, modification and fragmentation patterns in var...
www.nature.com
November 2, 2026 at 5:17 PM
Very happy to share that our work (beautifully led by @miemonti.bsky.social and #Hasan) is finally out! 😄We find that tRNAs engaged in actively translating ribosomes significantly differ from the 'total' pool available in the cell, specially upon stress! www.nature.com/articles/s41...
A new Nature Methods paper from one of our #LongTREC supervisors, @evamarianovoa.bsky.social , and colleagues introduces #tRIBO-seq. It’s a nanopore-based method that reads tRNAs bound to ribosomes while they make protein.
Read the full paper in Nature Methods now to learn more.
#tRNA #Nanopore
Read the full paper in Nature Methods now to learn more.
#tRNA #Nanopore
Very happy to share that our work (beautifully led by @miemonti.bsky.social and #Hasan) is finally out! 😄We find that tRNAs engaged in actively translating ribosomes significantly differ from the 'total' pool available in the cell, specially upon stress! www.nature.com/articles/s41...
Selective profiling of translationally active tRNAs and their dynamics under stress - Nature Methods
tRIBO-seq distinguishes between ribosome-associated tRNAs and total tRNAs using nanopore sequencing, thus revealing their potentially distinct abundance, modification and fragmentation patterns in var...
www.nature.com
November 3, 2026 at 8:57 AM
A new Nature Methods paper from one of our #LongTREC supervisors, @evamarianovoa.bsky.social , and colleagues introduces #tRIBO-seq. It’s a nanopore-based method that reads tRNAs bound to ribosomes while they make protein.
Read the full paper in Nature Methods now to learn more.
#tRNA #Nanopore
Read the full paper in Nature Methods now to learn more.
#tRNA #Nanopore
Happy to have played a small part by applying tRIBO-seq in mock and infected cells! @novoalab.bsky.social
April 24, 2026 at 11:09 AM
Happy to have played a small part by applying tRIBO-seq in mock and infected cells! @novoalab.bsky.social
Overall, tRIBO-seq provides a simple way to profile the active tRNAome, capturing abundance, modifications and fragmentation from actively translating ribosomes.
This project was co-led with Hasan Yilmaz, in collaboration between the Novoa Lab, IMMAGINA Biotech and the Soares Lab.
💃
This project was co-led with Hasan Yilmaz, in collaboration between the Novoa Lab, IMMAGINA Biotech and the Soares Lab.
💃
March 4, 2026 at 9:25 AM
Overall, tRIBO-seq provides a simple way to profile the active tRNAome, capturing abundance, modifications and fragmentation from actively translating ribosomes.
This project was co-led with Hasan Yilmaz, in collaboration between the Novoa Lab, IMMAGINA Biotech and the Soares Lab.
💃
This project was co-led with Hasan Yilmaz, in collaboration between the Novoa Lab, IMMAGINA Biotech and the Soares Lab.
💃
tRIBO-seq builds on RiboLace technology from to pull down active ribosomes.
Using ~15× less starting material, we recover comparable ribosome-associated tRNAs to polysome purification - with a much faster protocol.
Importantly, the recovered tRNAs correlate well with polysome-derived samples.
Using ~15× less starting material, we recover comparable ribosome-associated tRNAs to polysome purification - with a much faster protocol.
Importantly, the recovered tRNAs correlate well with polysome-derived samples.
March 4, 2026 at 9:25 AM
tRIBO-seq builds on RiboLace technology from to pull down active ribosomes.
Using ~15× less starting material, we recover comparable ribosome-associated tRNAs to polysome purification - with a much faster protocol.
Importantly, the recovered tRNAs correlate well with polysome-derived samples.
Using ~15× less starting material, we recover comparable ribosome-associated tRNAs to polysome purification - with a much faster protocol.
Importantly, the recovered tRNAs correlate well with polysome-derived samples.
To address this, we developed tRIBO-seq alongside @immagina.bsky.social.
The idea is simple:
• capture actively translating ribosomes
• isolate their associated native tRNAs
• sequence them using nanopore
This provides a direct view of the ribosome-associated tRNA population (ribo-tRNAs).
The idea is simple:
• capture actively translating ribosomes
• isolate their associated native tRNAs
• sequence them using nanopore
This provides a direct view of the ribosome-associated tRNA population (ribo-tRNAs).
March 4, 2026 at 9:25 AM
To address this, we developed tRIBO-seq alongside @immagina.bsky.social.
The idea is simple:
• capture actively translating ribosomes
• isolate their associated native tRNAs
• sequence them using nanopore
This provides a direct view of the ribosome-associated tRNA population (ribo-tRNAs).
The idea is simple:
• capture actively translating ribosomes
• isolate their associated native tRNAs
• sequence them using nanopore
This provides a direct view of the ribosome-associated tRNA population (ribo-tRNAs).
New preprint from
@novoalab.bsky.social !
Which tRNAs are used by ribosomes during translation?
We introduce tRIBO-seq, a nanopore method to sequence ribosome-associated tRNAs and track how the active tRNA pool changes across stress conditions.
www.biorxiv.org/content/10.6...
Thread 👇
@novoalab.bsky.social !
Which tRNAs are used by ribosomes during translation?
We introduce tRIBO-seq, a nanopore method to sequence ribosome-associated tRNAs and track how the active tRNA pool changes across stress conditions.
www.biorxiv.org/content/10.6...
Thread 👇
March 4, 2026 at 9:25 AM
New preprint from
@novoalab.bsky.social !
Which tRNAs are used by ribosomes during translation?
We introduce tRIBO-seq, a nanopore method to sequence ribosome-associated tRNAs and track how the active tRNA pool changes across stress conditions.
www.biorxiv.org/content/10.6...
Thread 👇
@novoalab.bsky.social !
Which tRNAs are used by ribosomes during translation?
We introduce tRIBO-seq, a nanopore method to sequence ribosome-associated tRNAs and track how the active tRNA pool changes across stress conditions.
www.biorxiv.org/content/10.6...
Thread 👇
tRIBO-seq builds on RiboLace technology to pull down active ribosomes.
Using ~15× less starting material, we recover comparable ribosome-associated tRNAs to polysome purification - with a much faster protocol.
Importantly, the recovered tRNAs correlate well with polysome-derived samples.
Using ~15× less starting material, we recover comparable ribosome-associated tRNAs to polysome purification - with a much faster protocol.
Importantly, the recovered tRNAs correlate well with polysome-derived samples.
March 4, 2026 at 9:16 AM
tRIBO-seq builds on RiboLace technology to pull down active ribosomes.
Using ~15× less starting material, we recover comparable ribosome-associated tRNAs to polysome purification - with a much faster protocol.
Importantly, the recovered tRNAs correlate well with polysome-derived samples.
Using ~15× less starting material, we recover comparable ribosome-associated tRNAs to polysome purification - with a much faster protocol.
Importantly, the recovered tRNAs correlate well with polysome-derived samples.
To address this, we developed tRIBO-seq alongside @immagina.bsky.social
The idea is simple:
• capture actively translating ribosomes
• isolate their associated native tRNAs
• sequence them using nanopore
This provides a direct view of the ribosome-associated tRNA population (ribo-tRNAs).
The idea is simple:
• capture actively translating ribosomes
• isolate their associated native tRNAs
• sequence them using nanopore
This provides a direct view of the ribosome-associated tRNA population (ribo-tRNAs).
March 4, 2026 at 9:16 AM
To address this, we developed tRIBO-seq alongside @immagina.bsky.social
The idea is simple:
• capture actively translating ribosomes
• isolate their associated native tRNAs
• sequence them using nanopore
This provides a direct view of the ribosome-associated tRNA population (ribo-tRNAs).
The idea is simple:
• capture actively translating ribosomes
• isolate their associated native tRNAs
• sequence them using nanopore
This provides a direct view of the ribosome-associated tRNA population (ribo-tRNAs).
📝 tRIBO-seq reveals how active tRNAs regulate translation under stress
✍️ RNA-Seq Blog
✍️ RNA-Seq Blog
tRIBO-seq reveals how active tRNAs regulate translation under stress
Article de RNA-Seq Blog
www.rna-seqblog.com
March 26, 2026 at 4:21 AM
📝 tRIBO-seq reveals how active tRNAs regulate translation under stress
✍️ RNA-Seq Blog
✍️ RNA-Seq Blog