vConTACT3 enables multirank, large-scale classification of eukaryotic and prokaryotic viruses.

In a groundbreaking advance for virology and bioinformatics, researchers have unveiled vConTACT3, a next-generation machine learning tool designed to revolutionize virus taxonomy on a global scale.

User genomes are provided to vConTACT3. ORFs are predicted with prodigal, and then sent to MMSeqs2 to be clustered at 5 identities (30%, 40%, 50%, 60%, 70%). These 5 clustering identities are used to build 5 separate protein cluster (PC)...

Viruses are key players in diverse ecosystems, but studying their impacts is technically and taxonomically challenging. Taxonomic complexities derive from undersampling, diverse DNA and RNA genomes with multiple evolutionary origins, and lack of a universal barcode gene. While virus ecogenomics has expanded access to and understanding of the virosphere, available classification tools poorly scale to modern discovery-based datasets, lack taxonomic resolution, and/or are unable to classify novel sequence space. Here we develop, benchmark, and release vConTACT3, a machine learning-based tool that improves scalability and accuracy, adds extensive user-requested features, expands classification to both eukaryote and prokaryote viruses for 4/6 officially recognized realms, and establishes accurate hierarchical taxonomy from genus to order. Application to 48,069 public virus genomes provided new taxonomy assignments for thousands of taxa, revealed support for fewer taxonomic ranks than currently available, and systematically identified taxonomically problematic areas across the virosphere. ### Competing Interest Statement The authors have declared no competing interest. U.S. National Science Foundation, https://ror.org/021nxhr62, DBI-2149506, DBI-2022070 Ohio Supercomputer Center, https://ror.org/01apna436 Deutsche Forschungsgemeinschaft, https://ror.org/018mejw64, EXC 2051 – Project-ID 390713860 European Research Council, https://ror.org/0472cxd90, Consolidator grant 865694 Alexander von Humboldt Foundation Biotechnology and Biological Sciences Research Council, BBS/E/F/000PR13631, BBS/E/F/000PR13633, BB/X011011/1, BBS/E/F/000PR13634, BBS/E/F/000PR13635, BBS/E/F/000PR13636

vConTACT3 enables multirank, large-scale classification of eukaryotic and prokaryotic viruses.

Viruses are key players in diverse ecosystems, but studying their impacts is technically and taxonomically challenging. Taxonomic complexities derive from undersampling, diverse DNA and RNA genomes wi...

Viruses are key players in diverse ecosystems, but studying their impacts is technically and taxonomically challenging. Taxonomic complexities derive from undersampling, diverse DNA and RNA genomes wi...

vConTACT3 enables multirank, large-scale classification of eukaryotic and prokaryotic viruses.

vConTACT3 enables multirank, large-scale classification of eukaryotic and prokaryotic viruses.

vConTACT3 enables multirank, large-scale classification of eukaryotic and prokaryotic viruses.