Ewa Zarnowska
@ewadorotaz.bsky.social
Ph.D. | Ex-Neuro & Ephys | Neuroscience | Lasers, Microscopes, Quantum | Coherent Corp. | Opinions mine
Pinned
Ewa Zarnowska
@ewadorotaz.bsky.social
· Sep 7
📣 Our abstract, “Enhanced High-Speed In Vivo Voltage Imaging Through Engineered Two-Photon Excitation,” was selected for #SfN26 NANO44 symposium: Imaging and Recording Neural Activity.
Nov. 18, 2026 | 8:00–11:30 AM
Abstract #4797
Thank you to my industrial team and academic collaborators! 🙌
Nov. 18, 2026 | 8:00–11:30 AM
Abstract #4797
Thank you to my industrial team and academic collaborators! 🙌
In honor to people who died in the terror. 25 years…
September 12, 2026 at 1:05 AM
In honor to people who died in the terror. 25 years…
📣 Our abstract, “Enhanced High-Speed In Vivo Voltage Imaging Through Engineered Two-Photon Excitation,” was selected for #SfN26 NANO44 symposium: Imaging and Recording Neural Activity.
Nov. 18, 2026 | 8:00–11:30 AM
Abstract #4797
Thank you to my industrial team and academic collaborators! 🙌
Nov. 18, 2026 | 8:00–11:30 AM
Abstract #4797
Thank you to my industrial team and academic collaborators! 🙌
September 7, 2026 at 6:28 PM
📣 Our abstract, “Enhanced High-Speed In Vivo Voltage Imaging Through Engineered Two-Photon Excitation,” was selected for #SfN26 NANO44 symposium: Imaging and Recording Neural Activity.
Nov. 18, 2026 | 8:00–11:30 AM
Abstract #4797
Thank you to my industrial team and academic collaborators! 🙌
Nov. 18, 2026 | 8:00–11:30 AM
Abstract #4797
Thank you to my industrial team and academic collaborators! 🙌
Reposted by Ewa Zarnowska
Oh wow! @thetransmitter.bsky.social amplified recent Bsky chatter about the dire US grant landscape.
Thank you, @emilysingerneuro.bsky.social 🙏.
Thank you, @emilysingerneuro.bsky.social 🙏.
Two years into one of the most tumultuous tenures in U.S. federal funding, researchers outline how they are coping with ongoing changes and reflect on the impact these changes are having on the system more broadly.
By @emilysingerneuro.bsky.social
www.thetransmitter.org/funding/amid...
By @emilysingerneuro.bsky.social
www.thetransmitter.org/funding/amid...
Amid funding uncertainty, neuroscientists report a mix of cynicism, persistence
Two years into one of the most tumultuous tenures in U.S. federal funding, researchers outline how they are coping with ongoing changes and reflect on the impact these changes are having on the system...
www.thetransmitter.org
August 28, 2026 at 7:27 PM
Oh wow! @thetransmitter.bsky.social amplified recent Bsky chatter about the dire US grant landscape.
Thank you, @emilysingerneuro.bsky.social 🙏.
Thank you, @emilysingerneuro.bsky.social 🙏.
Reposted by Ewa Zarnowska
Interesting work from the lab of Na Ji on how axial imaging resolution (3.5- 20 um) affects the extraction of accurate neuronal signals. Very instructive! www.biorxiv.org/content/10.6...
High Axial Resolution Is Necessary for Quantitative Two-Photon Calcium Imaging of Neuronal Populations
Two-photon calcium imaging is a standard tool for measuring neuronal population activity in vivo , yet how axial resolution, sensor expression strategy, and analysis pipeline jointly affect data accur...
www.biorxiv.org
August 18, 2026 at 7:26 PM
Interesting work from the lab of Na Ji on how axial imaging resolution (3.5- 20 um) affects the extraction of accurate neuronal signals. Very instructive! www.biorxiv.org/content/10.6...
(1-3) In recent bioRxiv article from Cristina Rodriguez's lab @Yale the authors measured both GDD and third-order dispersion (TOD) directly at the objective focal plane at 1300 and 1600 nm.
#3P #multiphoton
biorxiv.org/content/10.6...
#3P #multiphoton
biorxiv.org/content/10.6...
biorxiv.org
August 24, 2026 at 3:04 PM
(1-3) In recent bioRxiv article from Cristina Rodriguez's lab @Yale the authors measured both GDD and third-order dispersion (TOD) directly at the objective focal plane at 1300 and 1600 nm.
#3P #multiphoton
biorxiv.org/content/10.6...
#3P #multiphoton
biorxiv.org/content/10.6...
Attended, made many new friends, learned new things… quantum physics is applicable! Thanks to the organizers for a great time.
quantum.utdallas.edu/tqs2026/
quantum.utdallas.edu/tqs2026/
Texas Quantum Summit 2026
Overview The Texas Quantum Summit 2026 brings together leaders across academia, industry, and government to accelerate the development and deployment of quantum technologies in Texas and beyond. Build...
quantum.utdallas.edu
August 15, 2026 at 5:42 PM
Attended, made many new friends, learned new things… quantum physics is applicable! Thanks to the organizers for a great time.
quantum.utdallas.edu/tqs2026/
quantum.utdallas.edu/tqs2026/
I hope you’ll have a chance one day to see the images captured with Ventana. Also offered with its holographic optogenetics option and supported with Monaco laser. Ventana truly offers a window into the brain at scale! And the brilliant people behind it are another reason why it is so special. 🚀🧠🥳🤓
Check out this compact 920 nm ultrafast fs laser for 2p imaging of GCaMP, etc.. Mounted on its side like this, it takes up very little space on the table.
That's the Axon 920, generously loaned to the course thanks to @ewadorotaz.bsky.social and others at Coherent. It's what we use on our own rigs.
That's the Axon 920, generously loaned to the course thanks to @ewadorotaz.bsky.social and others at Coherent. It's what we use on our own rigs.
August 11, 2026 at 9:27 PM
I hope you’ll have a chance one day to see the images captured with Ventana. Also offered with its holographic optogenetics option and supported with Monaco laser. Ventana truly offers a window into the brain at scale! And the brilliant people behind it are another reason why it is so special. 🚀🧠🥳🤓
Glad I took this selfie before poster session opened this morning. It started at 9 and then it was 1 pm quickly. I did not drink, I did not eat. Thank you all who stopped by my poster at #FENS2026. I loved to talk to you all about lasers. Happy imaging.
July 10, 2026 at 2:08 PM
Glad I took this selfie before poster session opened this morning. It started at 9 and then it was 1 pm quickly. I did not drink, I did not eat. Thank you all who stopped by my poster at #FENS2026. I loved to talk to you all about lasers. Happy imaging.
Very interesting session this morning at #fens2026 on All-optical voltage imaging for neuronal circuits dissection. Few questions - how much more improvement can come from optimizing excitation source vs engineering better sensors? Are newer GEVIs shifting 2p excitation away from 920-940 nm range?
July 9, 2026 at 10:10 AM
Very interesting session this morning at #fens2026 on All-optical voltage imaging for neuronal circuits dissection. Few questions - how much more improvement can come from optimizing excitation source vs engineering better sensors? Are newer GEVIs shifting 2p excitation away from 920-940 nm range?
At #fens2026 and available to brainstorm with you how to improve two-photon image quality for fast imaging with photons craving fluorescent probes. Send me a msg and let’s chat indoors over iced coffee! Outdoor temp reached 37 C! 🫠
July 8, 2026 at 1:02 PM
At #fens2026 and available to brainstorm with you how to improve two-photon image quality for fast imaging with photons craving fluorescent probes. Send me a msg and let’s chat indoors over iced coffee! Outdoor temp reached 37 C! 🫠
Reposted by Ewa Zarnowska
Excited to share our first lab preprint! We enabled fast wide-field FLIM with low noise and high dynamic range. We built compact resonant modules for EO-FLIM that fit into your hand and developed methods to capture multi-exponential phasor plots at up to 500 Hz.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
June 8, 2026 at 11:03 PM
Excited to share our first lab preprint! We enabled fast wide-field FLIM with low noise and high dynamic range. We built compact resonant modules for EO-FLIM that fit into your hand and developed methods to capture multi-exponential phasor plots at up to 500 Hz.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Great few days at @mblscience.bsky.social supporting Neurobiology and NSB courses. It’s always exciting to see how ultrafast laser technology help enable new #neuroscience imaging methods. A big thank you to organizers, instructors, students, Coherent and others for making this event spectacular.
June 29, 2026 at 1:20 AM
Great few days at @mblscience.bsky.social supporting Neurobiology and NSB courses. It’s always exciting to see how ultrafast laser technology help enable new #neuroscience imaging methods. A big thank you to organizers, instructors, students, Coherent and others for making this event spectacular.
I’m going to spend a couple of days this week at @mblscience.bsky.social. Cool place, cool people and great science.
June 22, 2026 at 9:44 PM
I’m going to spend a couple of days this week at @mblscience.bsky.social. Cool place, cool people and great science.
Reposted by Ewa Zarnowska
If you're not replacing your PMTs every 6 months, you might as well opt for SiPMs.
src: www.nature.com/articles/s41...
sipm:
opg.optica.org/oe/fulltext....
www.nature.com/articles/s41...
src: www.nature.com/articles/s41...
sipm:
opg.optica.org/oe/fulltext....
www.nature.com/articles/s41...
June 11, 2026 at 11:47 PM
If you're not replacing your PMTs every 6 months, you might as well opt for SiPMs.
src: www.nature.com/articles/s41...
sipm:
opg.optica.org/oe/fulltext....
www.nature.com/articles/s41...
src: www.nature.com/articles/s41...
sipm:
opg.optica.org/oe/fulltext....
www.nature.com/articles/s41...
Reposted by Ewa Zarnowska
With #FENS2026 just around the corner, our unofficial
@fens.org abstract explorer is back:
fens2026.neuronaldynamics.eu
• Search abstracts by author, keyword, affiliation, and more
• Discover related work using abstract similarity
• Save favorites, add notes and tags, and build your own itinerary
@fens.org abstract explorer is back:
fens2026.neuronaldynamics.eu
• Search abstracts by author, keyword, affiliation, and more
• Discover related work using abstract similarity
• Save favorites, add notes and tags, and build your own itinerary
June 11, 2026 at 12:02 PM
With #FENS2026 just around the corner, our unofficial
@fens.org abstract explorer is back:
fens2026.neuronaldynamics.eu
• Search abstracts by author, keyword, affiliation, and more
• Discover related work using abstract similarity
• Save favorites, add notes and tags, and build your own itinerary
@fens.org abstract explorer is back:
fens2026.neuronaldynamics.eu
• Search abstracts by author, keyword, affiliation, and more
• Discover related work using abstract similarity
• Save favorites, add notes and tags, and build your own itinerary
Heading to Barcelona for my very first FENS! 🧠✨
Excited for the symposia, nerding out at posters, and presenting mine (PS07-10AM-005, Advanced Two-Photon Imaging Techniques, come say hi!).
Also accepting applications for churros con chocolate buddies :) #FENS2026
Excited for the symposia, nerding out at posters, and presenting mine (PS07-10AM-005, Advanced Two-Photon Imaging Techniques, come say hi!).
Also accepting applications for churros con chocolate buddies :) #FENS2026
June 11, 2026 at 7:31 PM
Heading to Barcelona for my very first FENS! 🧠✨
Excited for the symposia, nerding out at posters, and presenting mine (PS07-10AM-005, Advanced Two-Photon Imaging Techniques, come say hi!).
Also accepting applications for churros con chocolate buddies :) #FENS2026
Excited for the symposia, nerding out at posters, and presenting mine (PS07-10AM-005, Advanced Two-Photon Imaging Techniques, come say hi!).
Also accepting applications for churros con chocolate buddies :) #FENS2026
Reposted by Ewa Zarnowska
Here’s our latest preprint on uncovering neural control of a coordinated walking pattern! doi.org/10.64898/202... (author-list jumbled, see pdf for corrected). Video shows the central paradigm in this study, we optogenetically induce walking in headless flies and sequentially reduce proprioception.
May 4, 2026 at 8:07 PM
Here’s our latest preprint on uncovering neural control of a coordinated walking pattern! doi.org/10.64898/202... (author-list jumbled, see pdf for corrected). Video shows the central paradigm in this study, we optogenetically induce walking in headless flies and sequentially reduce proprioception.
Back from #OpticaBiophotonics26. I had an opportunity to share how we think about lasers and our laser solutions for emerging imaging applications.
May 2, 2026 at 9:32 PM
Back from #OpticaBiophotonics26. I had an opportunity to share how we think about lasers and our laser solutions for emerging imaging applications.
Multimodal alignments of in vivo imaging and spatial biology datasets at cellular resolution
#mini2p #Axon920 #laser
www.biorxiv.org/content/10.6...
#mini2p #Axon920 #laser
www.biorxiv.org/content/10.6...
www.biorxiv.org
May 2, 2026 at 9:25 PM
Multimodal alignments of in vivo imaging and spatial biology datasets at cellular resolution
#mini2p #Axon920 #laser
www.biorxiv.org/content/10.6...
#mini2p #Axon920 #laser
www.biorxiv.org/content/10.6...
Reposted by Ewa Zarnowska
PinkyCaMP is finally published!!
My first paper co-first authored with @sikmys.bsky.social where we make a great optogenetic and multiplex compatible red calcium indicator.
Thank you to @massecklab.bsky.social and @campbell-lab.bsky.social for guiding this journey
www.nature.com/articles/s41...
My first paper co-first authored with @sikmys.bsky.social where we make a great optogenetic and multiplex compatible red calcium indicator.
Thank you to @massecklab.bsky.social and @campbell-lab.bsky.social for guiding this journey
www.nature.com/articles/s41...
PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities - Nature Methods
PinkyCaMP is a red genetically encoded calcium indicator with improved brightness and photostability. Derived from mScarlet, it does not exhibit photoswitching upon blue light illumination and is ther...
www.nature.com
April 24, 2026 at 10:25 AM
PinkyCaMP is finally published!!
My first paper co-first authored with @sikmys.bsky.social where we make a great optogenetic and multiplex compatible red calcium indicator.
Thank you to @massecklab.bsky.social and @campbell-lab.bsky.social for guiding this journey
www.nature.com/articles/s41...
My first paper co-first authored with @sikmys.bsky.social where we make a great optogenetic and multiplex compatible red calcium indicator.
Thank you to @massecklab.bsky.social and @campbell-lab.bsky.social for guiding this journey
www.nature.com/articles/s41...
Reposted by Ewa Zarnowska
Open-source modular FPGA system for two-photon mesoscope: multiarea, multidepth neural activity recording. @riichirohira.bsky.social et al. present high throughput 2p calcium and what is maybe the largest field-of-view 2p fluorescence lifetime imaging (FLIM) data. doi.org/10.1117/1.NP...
April 24, 2026 at 6:40 PM
Open-source modular FPGA system for two-photon mesoscope: multiarea, multidepth neural activity recording. @riichirohira.bsky.social et al. present high throughput 2p calcium and what is maybe the largest field-of-view 2p fluorescence lifetime imaging (FLIM) data. doi.org/10.1117/1.NP...
I spent a bit more time thinking about this pre-print biorxiv.org/content/10.6...
👉3P isn't about more laser power. It's about the right rep rate, one pulse per pixel, and laser stability. If you are not measuring pulse-to-pulse noise you're not measuring your biology.
👉3P isn't about more laser power. It's about the right rep rate, one pulse per pixel, and laser stability. If you are not measuring pulse-to-pulse noise you're not measuring your biology.
biorxiv.org
April 21, 2026 at 12:17 AM
I spent a bit more time thinking about this pre-print biorxiv.org/content/10.6...
👉3P isn't about more laser power. It's about the right rep rate, one pulse per pixel, and laser stability. If you are not measuring pulse-to-pulse noise you're not measuring your biology.
👉3P isn't about more laser power. It's about the right rep rate, one pulse per pixel, and laser stability. If you are not measuring pulse-to-pulse noise you're not measuring your biology.
Practical optimization for three-photon microscopy to allow larger field of view and faster calcium imaging (10-30 Hz)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
April 14, 2026 at 6:45 PM
Practical optimization for three-photon microscopy to allow larger field of view and faster calcium imaging (10-30 Hz)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Easter time with my husband while our daughter is studying in PA
April 5, 2026 at 1:53 PM
Easter time with my husband while our daughter is studying in PA