@JohnGrossSF
@johngrosssf.bsky.social
Biochemist, aspiring cyclist, husband, empty nester with a dog. https://grosslab.ucsf.edu/
Congratulations to Gabriel Braun and collaborators Rakesh Kumar and Alan Hinnebusch for uncovering how deadenylase and decapping enzyme cofactors activate the DEAD-Box ATPase Dhh1. We wonder if this enzyme coordinates the 3' and 5' ends during mRNA turnover.
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Validate User
academic.oup.com
September 19, 2026 at 4:56 AM
Congratulations to Gabriel Braun and collaborators Rakesh Kumar and Alan Hinnebusch for uncovering how deadenylase and decapping enzyme cofactors activate the DEAD-Box ATPase Dhh1. We wonder if this enzyme coordinates the 3' and 5' ends during mRNA turnover.
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Reposted by @JohnGrossSF
Doing the lords work over here
Finally a more intuitaive way to learn pLDDT/pAE? 😎
sokrypton.github.io/protein_figh...
(Character idea from @hannes-stark.bsky.social & Alex Waldherr)
sokrypton.github.io/protein_figh...
(Character idea from @hannes-stark.bsky.social & Alex Waldherr)
September 14, 2026 at 8:32 PM
Doing the lords work over here
Reposted by @JohnGrossSF
Happy to share our new preprint on the structure of the human mitochondrial RNA degradosome, a central player in organellar RNA metabolism!
Work led by graduate student @paulafprado.bsky.social in our group.
www.biorxiv.org/content/10.6...
🧵 1/9
Work led by graduate student @paulafprado.bsky.social in our group.
www.biorxiv.org/content/10.6...
🧵 1/9
September 11, 2026 at 10:06 PM
Happy to share our new preprint on the structure of the human mitochondrial RNA degradosome, a central player in organellar RNA metabolism!
Work led by graduate student @paulafprado.bsky.social in our group.
www.biorxiv.org/content/10.6...
🧵 1/9
Work led by graduate student @paulafprado.bsky.social in our group.
www.biorxiv.org/content/10.6...
🧵 1/9
Reposted by @JohnGrossSF
Very excited to share our new preprint: “A pseudohelicase-centered complex couples assembly-dependent RNA cleavage to poly(UG)ylation”! 🎉 Want to know more? 🧵👇 www.biorxiv.org/content/10.6...
September 8, 2026 at 8:25 PM
Very excited to share our new preprint: “A pseudohelicase-centered complex couples assembly-dependent RNA cleavage to poly(UG)ylation”! 🎉 Want to know more? 🧵👇 www.biorxiv.org/content/10.6...
Reposted by @JohnGrossSF
⚡Preprint alert⚡ So proud of @amohamed98.bsky.social! Abdallah determined the cryo-EM structure of BRD4 bound to the kinase P-TEFb, defining the molecular basis of this key transcription interaction — and revealing that BRD4 and AFF4 binding are mutually exclusive to P-TEFb. Lots more inside 👇
September 8, 2026 at 5:25 PM
⚡Preprint alert⚡ So proud of @amohamed98.bsky.social! Abdallah determined the cryo-EM structure of BRD4 bound to the kinase P-TEFb, defining the molecular basis of this key transcription interaction — and revealing that BRD4 and AFF4 binding are mutually exclusive to P-TEFb. Lots more inside 👇
Reposted by @JohnGrossSF
If you or someone you know is interested in joining a fun and productive lab (JP Armache laboratory at Penn State University) to work on exciting projects, please follow or share the link below.
psu.wd1.myworkdayjobs.com/PSU_Academic...
psu.wd1.myworkdayjobs.com/PSU_Academic...
Postdoctoral Position
APPLICATION INSTRUCTIONS: CURRENT PENN STATE EMPLOYEE (faculty, staff, technical service, or student), please login to Workday to complete the internal application process. Please do not apply here, a...
psu.wd1.myworkdayjobs.com
July 8, 2026 at 8:53 PM
If you or someone you know is interested in joining a fun and productive lab (JP Armache laboratory at Penn State University) to work on exciting projects, please follow or share the link below.
psu.wd1.myworkdayjobs.com/PSU_Academic...
psu.wd1.myworkdayjobs.com/PSU_Academic...
Reposted by @JohnGrossSF
Out now in @science.org
Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion | Science www.science.org/doi/10.1126/...
Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion | Science www.science.org/doi/10.1126/...
Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion
Viruses are intracellular parasites that reprogram the host proteome to promote replication and evade immune recognition. We applied a virome-wide library of ~10,000 open reading frames to discover vi...
www.science.org
August 21, 2026 at 9:29 AM
Out now in @science.org
Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion | Science www.science.org/doi/10.1126/...
Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion | Science www.science.org/doi/10.1126/...
Reposted by @JohnGrossSF
PhIX-MS is out in Molecular Cell! Rapid in situ photo-crosslinking traps transient proteasome interactions before purification; crosslinking MS, cryo-EM and AlphaFold tell us where they bind.
Paper: doi.org/10.1016/j.mo...
Preview: doi.org/10.1016/j.mo...
Paper: doi.org/10.1016/j.mo...
Preview: doi.org/10.1016/j.mo...
August 14, 2026 at 5:40 PM
PhIX-MS is out in Molecular Cell! Rapid in situ photo-crosslinking traps transient proteasome interactions before purification; crosslinking MS, cryo-EM and AlphaFold tell us where they bind.
Paper: doi.org/10.1016/j.mo...
Preview: doi.org/10.1016/j.mo...
Paper: doi.org/10.1016/j.mo...
Preview: doi.org/10.1016/j.mo...
Congratulations to Yange Niu et al for showing how SIVsmm Vif was pre-equipped to antagonize human A3H-haplotype II through cryoEM and virological studies of the HIV-2 Vif-A3H complex. Another satisfying collaboration with Nicholas Chesarino and @memerman.bsky.social www.nature.com/articles/s41...
The structural mechanism of HIV-2 Vif antagonism of human APOBEC3H - Nature Communications
HIV-2 Vif counteracts a broad range of APOBEC3 restriction factors to promote viral infectivity. Here, the authors determine the structure of HIV-2 Vif bound to human APOBEC3H and the host cofactor CB...
www.nature.com
August 10, 2026 at 4:54 PM
Congratulations to Yange Niu et al for showing how SIVsmm Vif was pre-equipped to antagonize human A3H-haplotype II through cryoEM and virological studies of the HIV-2 Vif-A3H complex. Another satisfying collaboration with Nicholas Chesarino and @memerman.bsky.social www.nature.com/articles/s41...
Reposted by @JohnGrossSF
We are actively looking for a NMR facility manager for our growing NMR needs in Syracuse! This facility hosts an 800 with TCI cryoprobe (perfect for disordered protein research) and 600 with Prodigy cryoprobe. Please share with your communities!
Instructional Support Specialist (NMR Specialist) College of Environmental Science and Forestry, Syracuse, New York esf.interviewexchange.com/jobofferdeta... #NMRjobs #NMRchat #NMR 🧲
July 30, 2026 at 12:25 PM
We are actively looking for a NMR facility manager for our growing NMR needs in Syracuse! This facility hosts an 800 with TCI cryoprobe (perfect for disordered protein research) and 600 with Prodigy cryoprobe. Please share with your communities!
Strain-specific epistasis shapes fitness landscapes of APOBEC3G antagonism by HIV-1 Vif proteins | Science Advances www.science.org/doi/10.1126/...
Congratulations on this beautiful work!
Congratulations on this beautiful work!
Strain-specific epistasis shapes fitness landscapes of APOBEC3G antagonism by HIV-1 Vif proteins
Unexpected flexibility and evolutionary constraints shape how HIV-1 overcomes a key human antiviral protein.
www.science.org
July 24, 2026 at 12:12 AM
Strain-specific epistasis shapes fitness landscapes of APOBEC3G antagonism by HIV-1 Vif proteins | Science Advances www.science.org/doi/10.1126/...
Congratulations on this beautiful work!
Congratulations on this beautiful work!
Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion | Science
www.science.org/doi/full/10....
Viruses don't play by the same rules as host proteins: a CRISPR screen shows rotavirus NSP1 hijacks CUL3 using ELOBC as a surrogate for BTB protein, backed up by cryoEM!
www.science.org/doi/full/10....
Viruses don't play by the same rules as host proteins: a CRISPR screen shows rotavirus NSP1 hijacks CUL3 using ELOBC as a surrogate for BTB protein, backed up by cryoEM!
www.science.org
July 14, 2026 at 11:22 PM
Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion | Science
www.science.org/doi/full/10....
Viruses don't play by the same rules as host proteins: a CRISPR screen shows rotavirus NSP1 hijacks CUL3 using ELOBC as a surrogate for BTB protein, backed up by cryoEM!
www.science.org/doi/full/10....
Viruses don't play by the same rules as host proteins: a CRISPR screen shows rotavirus NSP1 hijacks CUL3 using ELOBC as a surrogate for BTB protein, backed up by cryoEM!
Reposted by @JohnGrossSF
💥 Final version of our Elp3 paper is now live @natcomms.nature.com! We show how the radical SAM enzyme Elp3 uses a molecular tunnel to transport acetate molecules and modify tRNA! We think our proposed mechanism answers long-standing questions about how this important enzyme works! rdcu.be/fq2bw
Elp3 uses a conserved molecular tunnel to transport acetate between distant active sites and catalyze tRNA wobble base modification
Nature Communications - tRNA modifications are essential for accurate translation, but how Elp3 catalyses them across distant active sites was unclear. Here, the authors show that Elp3 uses a...
rdcu.be
June 29, 2026 at 2:23 PM
💥 Final version of our Elp3 paper is now live @natcomms.nature.com! We show how the radical SAM enzyme Elp3 uses a molecular tunnel to transport acetate molecules and modify tRNA! We think our proposed mechanism answers long-standing questions about how this important enzyme works! rdcu.be/fq2bw
Reposted by @JohnGrossSF
In the midst of hate, I found there was, within me, an invincible love.
In the midst of tears, I found there was, within me, an invincible smile.
In the midst of chaos, I found there was, within me, an invincible calm.
- Albert Camus
#Poetry 💙📚👀
In the midst of tears, I found there was, within me, an invincible smile.
In the midst of chaos, I found there was, within me, an invincible calm.
- Albert Camus
#Poetry 💙📚👀
June 9, 2026 at 7:34 AM
In the midst of hate, I found there was, within me, an invincible love.
In the midst of tears, I found there was, within me, an invincible smile.
In the midst of chaos, I found there was, within me, an invincible calm.
- Albert Camus
#Poetry 💙📚👀
In the midst of tears, I found there was, within me, an invincible smile.
In the midst of chaos, I found there was, within me, an invincible calm.
- Albert Camus
#Poetry 💙📚👀
Reposted by @JohnGrossSF
I was told that I needed to request permission to collaborate with physicians from European countries before testing the effects of patient mutations they identified on an enzyme we study. For each collaborator, NIH is asking for email, institution, street address, phone number (!!). Insane.
This is fucking insanity
www.insidehighered.com/news/quick-t...
www.insidehighered.com/news/quick-t...
Report: NIH, NASA Restrict Co-Authoring With Foreign Scientists
The National Institutes of Health and the National Aeronautics and Space Administration are targeting collaborations between U.S. scientists and foreign researchers, Science reports.
www.insidehighered.com
May 22, 2026 at 5:13 PM
I was told that I needed to request permission to collaborate with physicians from European countries before testing the effects of patient mutations they identified on an enzyme we study. For each collaborator, NIH is asking for email, institution, street address, phone number (!!). Insane.
Reposted by @JohnGrossSF
Delighted to share our lab's latest (w/ inimitable @genophoria.bsky.social) in final form at @nature.com. Enormous lift by Sean Wang, @palindromephd.bsky.social & @martyyang.bsky.social to address extensive & constructive reviewer comments & see this through. (1/n) www.nature.com/articles/s41...
Pervasive and programmed nucleosome distortion on single chromatin fibres - Nature
An analytical pipeline called Iteratively Defined Lengths of Inaccessibility (IDLI) maps the genome-wide occupancy of a range of nucleosome types and shows that most nucleosomes exhibit programmed ‘di...
www.nature.com
May 6, 2026 at 2:32 PM
Delighted to share our lab's latest (w/ inimitable @genophoria.bsky.social) in final form at @nature.com. Enormous lift by Sean Wang, @palindromephd.bsky.social & @martyyang.bsky.social to address extensive & constructive reviewer comments & see this through. (1/n) www.nature.com/articles/s41...
Reposted by @JohnGrossSF
Conformational Ensembles of the Disordered 4E-BP2:eIF4E Complex Restrained by smFRET Experiments https://www.biorxiv.org/content/10.64898/2026.04.21.719986v1
April 24, 2026 at 9:48 PM
Conformational Ensembles of the Disordered 4E-BP2:eIF4E Complex Restrained by smFRET Experiments https://www.biorxiv.org/content/10.64898/2026.04.21.719986v1
Reposted by @JohnGrossSF
Interested in transcriptional regulation, enhancers and 3D genome folding?
In this new study we wondered about the role of cohesin loading at enhancers for long-range transcriptional control
www.biorxiv.org/content/10.6...
detailed 🧵👇
In this new study we wondered about the role of cohesin loading at enhancers for long-range transcriptional control
www.biorxiv.org/content/10.6...
detailed 🧵👇
www.biorxiv.org
February 12, 2026 at 9:39 PM
Interested in transcriptional regulation, enhancers and 3D genome folding?
In this new study we wondered about the role of cohesin loading at enhancers for long-range transcriptional control
www.biorxiv.org/content/10.6...
detailed 🧵👇
In this new study we wondered about the role of cohesin loading at enhancers for long-range transcriptional control
www.biorxiv.org/content/10.6...
detailed 🧵👇
Reposted by @JohnGrossSF
A herculean effort by many, but esp. the first three authors: Ujjwal Rathore, Eli Dugan, and Hunter Thornton working in the Krogan and Marson labs, with a veritable army of collaborators from HARC (harc.ucsf.edu) and beyond.
Press release: gladstone.org/news/scienti... (inc. paper link)
Press release: gladstone.org/news/scienti... (inc. paper link)
April 20, 2026 at 11:11 PM
A herculean effort by many, but esp. the first three authors: Ujjwal Rathore, Eli Dugan, and Hunter Thornton working in the Krogan and Marson labs, with a veritable army of collaborators from HARC (harc.ucsf.edu) and beyond.
Press release: gladstone.org/news/scienti... (inc. paper link)
Press release: gladstone.org/news/scienti... (inc. paper link)
Reposted by @JohnGrossSF
What would you think to be thus used? This is the strangers case; And this your mountainish inhumanity.
Sir Ian McKellen performing a monologue from Shakespeare’s Sir Thomas More on the Stephen Colbert show. Never have I heard this monologue performed with such a keen sense of prescience. Nor have I ever been in this exact historical moment.TY Sir Ian, for reaching us once again.
#Pinks #ProudBlue
#Pinks #ProudBlue
February 5, 2026 at 2:34 PM
What would you think to be thus used? This is the strangers case; And this your mountainish inhumanity.
Reposted by @JohnGrossSF
Our paper is (finally) out in Cell today!
CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis
www.cell.com/cell/fulltex...
Great collaborative effort - read more from first author @asamelson.bsky.social below:
CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis
www.cell.com/cell/fulltex...
Great collaborative effort - read more from first author @asamelson.bsky.social below:
January 28, 2026 at 5:24 PM
Our paper is (finally) out in Cell today!
CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis
www.cell.com/cell/fulltex...
Great collaborative effort - read more from first author @asamelson.bsky.social below:
CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis
www.cell.com/cell/fulltex...
Great collaborative effort - read more from first author @asamelson.bsky.social below:
Reposted by @JohnGrossSF
🧪 Russel Vought wants to kill the #NIH, in a slow, sneaky way. The NIH appropriations include language that allows multi-year funding of grants. No 40% cut.
Yay. No.
Allowing multi-year funding allows fewer grants will be funded. This toxic compromise will kill science.
We deserve better.
Yay. No.
Allowing multi-year funding allows fewer grants will be funded. This toxic compromise will kill science.
We deserve better.
If we continue to compromise with people whose stated goal is to destroy NIH, then we will not be successful in saving it.
January 21, 2026 at 12:37 AM
🧪 Russel Vought wants to kill the #NIH, in a slow, sneaky way. The NIH appropriations include language that allows multi-year funding of grants. No 40% cut.
Yay. No.
Allowing multi-year funding allows fewer grants will be funded. This toxic compromise will kill science.
We deserve better.
Yay. No.
Allowing multi-year funding allows fewer grants will be funded. This toxic compromise will kill science.
We deserve better.
"One has not only a legal but a moral responsibility to obey just laws. Conversely, one has a moral responsibility to disobey unjust laws.” Dr. MLK Jr, Letter from a Birmingham Jail
January 19, 2026 at 7:32 PM
"One has not only a legal but a moral responsibility to obey just laws. Conversely, one has a moral responsibility to disobey unjust laws.” Dr. MLK Jr, Letter from a Birmingham Jail
"The arc of the moral universe is long, but it bends toward justice.” Dr. Martin Luther King Jr.
January 17, 2026 at 7:31 PM
"The arc of the moral universe is long, but it bends toward justice.” Dr. Martin Luther King Jr.
Reposted by @JohnGrossSF
We are thrilled to share our latest work uncovering the mechanistic basis of target-directed microRNA degradation (TDMD). This work was driven by @jakobfarnung.bsky.social and @elenaslo.bsky.social in a fantastic collaboration with Brenda Schulman's lab. tinyurl.com/E3TDMD (1/5)
January 6, 2026 at 3:16 PM
We are thrilled to share our latest work uncovering the mechanistic basis of target-directed microRNA degradation (TDMD). This work was driven by @jakobfarnung.bsky.social and @elenaslo.bsky.social in a fantastic collaboration with Brenda Schulman's lab. tinyurl.com/E3TDMD (1/5)