Matthieu Sainlos
@ch3w13.bsky.social
Chemical biology - Protein engineering -
Neuroscience
Neuroscience
Reposted by Matthieu Sainlos
This work is now out at @pnas.org
In drug discovery it’s often said selectivity is key - and here is a solution!
Our PANCS platforms continue to expand!
www.pnas.org/doi/10.1073/...
In drug discovery it’s often said selectivity is key - and here is a solution!
Our PANCS platforms continue to expand!
www.pnas.org/doi/10.1073/...
June 2, 2026 at 7:12 PM
This work is now out at @pnas.org
In drug discovery it’s often said selectivity is key - and here is a solution!
Our PANCS platforms continue to expand!
www.pnas.org/doi/10.1073/...
In drug discovery it’s often said selectivity is key - and here is a solution!
Our PANCS platforms continue to expand!
www.pnas.org/doi/10.1073/...
Reposted by Matthieu Sainlos
The past, present and future of de novo protein design | Nature share.google/hkTfYehZCm2D...
April 29, 2026 at 5:56 PM
The past, present and future of de novo protein design | Nature share.google/hkTfYehZCm2D...
Reposted by Matthieu Sainlos
Artificial allosteric protein switches with machine-learning-designed receptors - @arccoesb.bsky.social go.nature.com/4cshpM3
Artificial allosteric protein switches with machine-learning-designed receptors - Nature Biotechnology
Allosteric biosensors are constructed by combining reporter proteins with machine-learning-designed receptor domains.
go.nature.com
April 15, 2026 at 2:50 PM
Artificial allosteric protein switches with machine-learning-designed receptors - @arccoesb.bsky.social go.nature.com/4cshpM3
Reposted by Matthieu Sainlos
Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...
A series of spontaneously blinking dyes for super-resolution microscopy - Nature Methods
A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...
www.nature.com
April 15, 2026 at 7:52 PM
Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...
Reposted by Matthieu Sainlos
☕ @mathilda95.bsky.social @nadlerlab.bsky.social & co introduce a correlative light and electron #microscopy workflow, Lipid-CLEM, combining near-native #lipid probes and on-section labelling via click chemistry. Lipid-CLEM quantitatively analyses lipids in membrane nanodomains.
bit.ly/4moYLJH
bit.ly/4moYLJH
Visualizing suborganellar lipid distribution using correlative light and electron microscopy - Nature Cell Biology
Lennartz et al. introduce a correlative light and electron microscopy workflow, Lipid-CLEM, combining near-native lipid probes and on-section labelling via click chemistry. Lipid-CLEM quantitatively a...
bit.ly
April 13, 2026 at 3:21 PM
☕ @mathilda95.bsky.social @nadlerlab.bsky.social & co introduce a correlative light and electron #microscopy workflow, Lipid-CLEM, combining near-native #lipid probes and on-section labelling via click chemistry. Lipid-CLEM quantitatively analyses lipids in membrane nanodomains.
bit.ly/4moYLJH
bit.ly/4moYLJH
Reposted by Matthieu Sainlos
Glad to see this out in the open! Preprint from Anna Brachet and colleagues from @iins-bordeaux.bsky.social looking at the relative organization and roles of ßII-/ßIII-spectrin in the dendritic membrane-associated periodic skeleton (MPS), including cool MINFLUX data: doi.org/10.64898/202...
April 3, 2026 at 7:14 AM
Glad to see this out in the open! Preprint from Anna Brachet and colleagues from @iins-bordeaux.bsky.social looking at the relative organization and roles of ßII-/ßIII-spectrin in the dendritic membrane-associated periodic skeleton (MPS), including cool MINFLUX data: doi.org/10.64898/202...
Reposted by Matthieu Sainlos
A high-affinity split-HaloTag for live-cell protein labeling. And much more.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
A high-affinity split-HaloTag for live-cell protein labeling
Nature Communications - Lin and colleagues present high-affinity split-HaloTag pairs for protein tagging and multiplexed labelling. This versatile system allows protein visualisation with diverse...
www.nature.com
March 26, 2026 at 3:39 PM
A high-affinity split-HaloTag for live-cell protein labeling. And much more.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Matthieu Sainlos
It’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density. Lipid-CLEM, now out in @natcellbio.nature.com brought to you by @mathilda95.bsky.social changes that:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
March 23, 2026 at 9:11 AM
It’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density. Lipid-CLEM, now out in @natcellbio.nature.com brought to you by @mathilda95.bsky.social changes that:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Matthieu Sainlos
Check out our new review on binder discovery! In a fast-moving world, here are some thoughts we have in the moment.
Congrats @jzy2799.bsky.social and Eddy!!
www.sciencedirect.com/science/arti...
Congrats @jzy2799.bsky.social and Eddy!!
www.sciencedirect.com/science/arti...
Toward universal binder discovery: Advances in display, computational design and in vivo platforms
Protein binders are fundamental tools in chemical biology, key components of biotechnologies, and the foundation of biologics-based medicines. However…
www.sciencedirect.com
March 6, 2026 at 11:08 PM
Check out our new review on binder discovery! In a fast-moving world, here are some thoughts we have in the moment.
Congrats @jzy2799.bsky.social and Eddy!!
www.sciencedirect.com/science/arti...
Congrats @jzy2799.bsky.social and Eddy!!
www.sciencedirect.com/science/arti...
Reposted by Matthieu Sainlos
A new @pnas.org study caught the eye of our bioinformatics team with its identification of ~4,500 targetable binding sites & binding seeds across human cell surface proteins—giving IPI and other labs a protein design launch point.
Mapping targetable sites on the human surfaceome for the design of novel binders | PNAS
The human cell surfaceome, integral to cell communication and disease mechanisms, presents a prime target for therapeutic intervention. De novo pro...
buff.ly
February 26, 2026 at 4:05 PM
A new @pnas.org study caught the eye of our bioinformatics team with its identification of ~4,500 targetable binding sites & binding seeds across human cell surface proteins—giving IPI and other labs a protein design launch point.
Reposted by Matthieu Sainlos
The final version of our new paper is out now - and open access @acs.org Central Science!!
Such a fun collaboration!
pubs.acs.org/doi/10.1021/...
Such a fun collaboration!
pubs.acs.org/doi/10.1021/...
January 25, 2026 at 1:05 AM
The final version of our new paper is out now - and open access @acs.org Central Science!!
Such a fun collaboration!
pubs.acs.org/doi/10.1021/...
Such a fun collaboration!
pubs.acs.org/doi/10.1021/...
Reposted by Matthieu Sainlos
Reposted by Matthieu Sainlos
Check out our newest work! This is a story on how to get selectivity in binders - both isoform and site selectivity. Read the paper or enjoy this brief Skytorial of what we did!
www.biorxiv.org/content/10.1...
1/n
www.biorxiv.org/content/10.1...
1/n
PANCS-spec-Binders: A system for rapidly discovering isoform- or epitope-specific binders
Proteins that bind to a target protein of interest, termed "binders," are essential components of biological research reagents and therapeutics. Target proteins present multiple binding surfaces with ...
www.biorxiv.org
November 19, 2025 at 6:10 PM
Check out our newest work! This is a story on how to get selectivity in binders - both isoform and site selectivity. Read the paper or enjoy this brief Skytorial of what we did!
www.biorxiv.org/content/10.1...
1/n
www.biorxiv.org/content/10.1...
1/n
Reposted by Matthieu Sainlos
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 13, 2025 at 4:20 PM
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Matthieu Sainlos
Registration for THE chemical biology conference of 2026 is now open! EMBO ChemBio 2026 in Heidelberg
DeGrado, Arikin, Picotti (Keynotes). @lmkdassama.bsky.social @brianliau.bsky.social @rhodamine110.bsky.social @benlehner.bsky.social @alitavassoli.bsky.social
www.embl.org/about/info/c...
DeGrado, Arikin, Picotti (Keynotes). @lmkdassama.bsky.social @brianliau.bsky.social @rhodamine110.bsky.social @benlehner.bsky.social @alitavassoli.bsky.social
www.embl.org/about/info/c...
Chemical biology 2026
www.embl.org
November 11, 2025 at 5:46 PM
Registration for THE chemical biology conference of 2026 is now open! EMBO ChemBio 2026 in Heidelberg
DeGrado, Arikin, Picotti (Keynotes). @lmkdassama.bsky.social @brianliau.bsky.social @rhodamine110.bsky.social @benlehner.bsky.social @alitavassoli.bsky.social
www.embl.org/about/info/c...
DeGrado, Arikin, Picotti (Keynotes). @lmkdassama.bsky.social @brianliau.bsky.social @rhodamine110.bsky.social @benlehner.bsky.social @alitavassoli.bsky.social
www.embl.org/about/info/c...
Reposted by Matthieu Sainlos
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
October 27, 2025 at 9:43 PM
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
Reposted by Matthieu Sainlos
I am thrilled to release ProteinDJ: a high-performance and modular protein design pipeline. Our open-source workflow incorporates #RFdiffusion, #ProteinMPNN, #FAMPNN, #AlphaFold2 and #Boltz-2. It is a fast, free, and fun way to design proteins (1/5)
doi.org/10.1101/2025.09.24.678028 #proteindesign
doi.org/10.1101/2025.09.24.678028 #proteindesign
September 28, 2025 at 9:16 PM
I am thrilled to release ProteinDJ: a high-performance and modular protein design pipeline. Our open-source workflow incorporates #RFdiffusion, #ProteinMPNN, #FAMPNN, #AlphaFold2 and #Boltz-2. It is a fast, free, and fun way to design proteins (1/5)
doi.org/10.1101/2025.09.24.678028 #proteindesign
doi.org/10.1101/2025.09.24.678028 #proteindesign
Reposted by Matthieu Sainlos
Paralog-specific intrabodies for PSD-93 and SAP102 expand the molecular toolkit to resolve excitatory synapse organization https://www.biorxiv.org/content/10.1101/2025.08.24.671450v1
August 26, 2025 at 12:46 AM
Paralog-specific intrabodies for PSD-93 and SAP102 expand the molecular toolkit to resolve excitatory synapse organization https://www.biorxiv.org/content/10.1101/2025.08.24.671450v1
Reposted by Matthieu Sainlos
Also check out the associated News & Views by Masayasu Taki and Masayoshi Nakamura highlighting the work from @kjohnsson.bsky.social lab on SNAP-tag2
www.nature.com/articles/s41...
www.nature.com/articles/s41...
July 8, 2025 at 4:02 PM
Also check out the associated News & Views by Masayasu Taki and Masayoshi Nakamura highlighting the work from @kjohnsson.bsky.social lab on SNAP-tag2
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Matthieu Sainlos
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
www.nature.com
July 3, 2025 at 7:45 PM
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
Reposted by Matthieu Sainlos
We are thrilled to welcome Barbara Imperiali at #ChemBioParis2025 in Paris, October 6-9, 2025. Sign up here: chembioparis2025.com – Early bird ends June 10 with reduced fees for @icbschembio.bsky.social - euchems.eu - @eu-openscreen.bsky.social - @scf-chembio.bsky.social - #ChemBioEvent
May 1, 2025 at 11:19 AM
We are thrilled to welcome Barbara Imperiali at #ChemBioParis2025 in Paris, October 6-9, 2025. Sign up here: chembioparis2025.com – Early bird ends June 10 with reduced fees for @icbschembio.bsky.social - euchems.eu - @eu-openscreen.bsky.social - @scf-chembio.bsky.social - #ChemBioEvent
Reposted by Matthieu Sainlos
Employing engineered split inteins to replace the internal regions of target proteins in a single reaction. www.science.org/doi/10.1126/... #NBThighlight
Protein editing using a coordinated transposition reaction
Protein engineering through the ligation of polypeptide fragments has proven enormously powerful for studying biochemical processes. In general, this strategy necessitates a final protein-folding step...
www.science.org
April 4, 2025 at 1:17 PM
Employing engineered split inteins to replace the internal regions of target proteins in a single reaction. www.science.org/doi/10.1126/... #NBThighlight
Reposted by Matthieu Sainlos
Maglia and colleagues discuss advances in nanopore technology en route to single-molecule protein sequencing go.nature.com/3FL0g3k
rdcu.be/efeZR
rdcu.be/efeZR
Toward single-molecule protein sequencing using nanopores - Nature Biotechnology
Maglia and colleagues discuss advances in nanopore technology en route to single-molecule protein sequencing
go.nature.com
April 1, 2025 at 4:04 AM
Maglia and colleagues discuss advances in nanopore technology en route to single-molecule protein sequencing go.nature.com/3FL0g3k
rdcu.be/efeZR
rdcu.be/efeZR