#NoTimeToDie! @inflammasomelab, Holley, Monteleone &co show @natimmunol.bsky.social that cells that die by #pyroptosis, or #necroptosis, are crowned with F-actin filopodia that makes them visible by dendritic cells via CLEC9A to initiate adaptive immunity! www.nature.com/articles/s41...
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells - Nature Immunology
Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate dendritic cells.
www.nature.com
December 5, 2024 at 4:55 PM
#NoTimeToDie! @inflammasomelab, Holley, Monteleone &co show @natimmunol.bsky.social that cells that die by #pyroptosis, or #necroptosis, are crowned with F-actin filopodia that makes them visible by dendritic cells via CLEC9A to initiate adaptive immunity! www.nature.com/articles/s41...
Delighted that this study is finally out @embojournal.org. A detailed analysis of how DNGR-1/CLEC9A signals to promote cross-presentation of dead cell-associated antigens yet does not activate dendritic cells.
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
DNGR-1 signalling limits dendritic cell activation for optimal antigen cross-presentation | The EMBO Journal
imageimageDNGR-1 (CLEC9A) is a C-type lectin receptor of type-1 conventional dendritic cells (cDC1s) with an important role in cross-presentation of dead cell antigens. This study explores how it equips cDC1s with the ability to cross-present such ...
www.embopress.org
November 3, 2025 at 5:23 PM
Delighted that this study is finally out @embojournal.org. A detailed analysis of how DNGR-1/CLEC9A signals to promote cross-presentation of dead cell-associated antigens yet does not activate dendritic cells.
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
Have you ever wondered whether pyroptotic corpses have functions beyond the grave? We made the surprising discovery that pyroptotic corpses are flagged by #filopodia to alert #DendriticCells they need autopsy. A short Bluetorial (1/13)👇with movies!
Link: rdcu.be/d2mjm
Link: rdcu.be/d2mjm
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells
Nature Immunology - Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate...
rdcu.be
December 9, 2024 at 1:39 AM
Have you ever wondered whether pyroptotic corpses have functions beyond the grave? We made the surprising discovery that pyroptotic corpses are flagged by #filopodia to alert #DendriticCells they need autopsy. A short Bluetorial (1/13)👇with movies!
Link: rdcu.be/d2mjm
Link: rdcu.be/d2mjm
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells @natureportfolio.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells - Nature Immunology
Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate dendritic cells.
www.nature.com
December 4, 2024 at 1:38 PM
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells @natureportfolio.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Promising vaccine approach can induce longer-lasting protection against Covid & eliminate need for boosters. Experimental vax is a 2-piece construction. A key portion of SARSCoV2's spike is fused to the Clec9A antibody. The antibody boosts a subset of immune cells medicalxpress.com/news/2024-06...
June 5, 2024 at 8:03 AM
Promising vaccine approach can induce longer-lasting protection against Covid & eliminate need for boosters. Experimental vax is a 2-piece construction. A key portion of SARSCoV2's spike is fused to the Clec9A antibody. The antibody boosts a subset of immune cells medicalxpress.com/news/2024-06...
😃 The PacMan of dead cells - a review on CLEC9a, a critical receptor on a critical subset of DCs nature.com/articles/...
February 16, 2025 at 1:00 PM
😃 The PacMan of dead cells - a review on CLEC9a, a critical receptor on a critical subset of DCs nature.com/articles/...
DNGR-1 signalling limits dendritic cell activation for optimal antigen cross-presentation
@reisesousalab.bsky.social et al implicate recruitment of phosphatase SHIP1 by receptor DNGR-1/CLEC9A in uncoupling of cross-presentation from inflammatory induction
www.embopress.org/doi/full/10....
@reisesousalab.bsky.social et al implicate recruitment of phosphatase SHIP1 by receptor DNGR-1/CLEC9A in uncoupling of cross-presentation from inflammatory induction
www.embopress.org/doi/full/10....
November 6, 2025 at 9:02 AM
DNGR-1 signalling limits dendritic cell activation for optimal antigen cross-presentation
@reisesousalab.bsky.social et al implicate recruitment of phosphatase SHIP1 by receptor DNGR-1/CLEC9A in uncoupling of cross-presentation from inflammatory induction
www.embopress.org/doi/full/10....
@reisesousalab.bsky.social et al implicate recruitment of phosphatase SHIP1 by receptor DNGR-1/CLEC9A in uncoupling of cross-presentation from inflammatory induction
www.embopress.org/doi/full/10....
🩸How is cDCpoiesis regulated?
DNGR-1, or CLEC9A, most known as a cDC1 receptor for cross-presentation of dead-cell associated antigens - may play a role here.
🔬Great to see labour of love led by Ana finally out now in @jem.org.
Available open access:
hubs.la/Q03mg6Sc0
@reisesousalab.bsky.social
DNGR-1, or CLEC9A, most known as a cDC1 receptor for cross-presentation of dead-cell associated antigens - may play a role here.
🔬Great to see labour of love led by Ana finally out now in @jem.org.
Available open access:
hubs.la/Q03mg6Sc0
@reisesousalab.bsky.social
May 15, 2025 at 8:11 AM
🩸How is cDCpoiesis regulated?
DNGR-1, or CLEC9A, most known as a cDC1 receptor for cross-presentation of dead-cell associated antigens - may play a role here.
🔬Great to see labour of love led by Ana finally out now in @jem.org.
Available open access:
hubs.la/Q03mg6Sc0
@reisesousalab.bsky.social
DNGR-1, or CLEC9A, most known as a cDC1 receptor for cross-presentation of dead-cell associated antigens - may play a role here.
🔬Great to see labour of love led by Ana finally out now in @jem.org.
Available open access:
hubs.la/Q03mg6Sc0
@reisesousalab.bsky.social
Summary: pyroptotic & necroptotic cells assemble F-actin-rich filopodia to ⛳️ their corpses. Filopodia mark corpses for recognition by #DCs, via the corpse antigen sampling receptor, #CLEC9A.
New questions: Does this trigger Ag-specific immunity in #infection, #vaccination & #autoimmunity? 🤯 (12/13)
New questions: Does this trigger Ag-specific immunity in #infection, #vaccination & #autoimmunity? 🤯 (12/13)
December 9, 2024 at 1:39 AM
Summary: pyroptotic & necroptotic cells assemble F-actin-rich filopodia to ⛳️ their corpses. Filopodia mark corpses for recognition by #DCs, via the corpse antigen sampling receptor, #CLEC9A.
New questions: Does this trigger Ag-specific immunity in #infection, #vaccination & #autoimmunity? 🤯 (12/13)
New questions: Does this trigger Ag-specific immunity in #infection, #vaccination & #autoimmunity? 🤯 (12/13)
🎉 Max Schaaf defends his PhD at @mpip-mainz.mpg.de !
Developed smart #liposomes with polymer-based surfaces for targeted immune cell delivery: anti-CLEC9A antibodies, stable diABZI loading, in vivo efficacy.
A leap forward in #nanomedicine & #Polymer #Engineering. #Immunotherapy #DrugDelivery
Developed smart #liposomes with polymer-based surfaces for targeted immune cell delivery: anti-CLEC9A antibodies, stable diABZI loading, in vivo efficacy.
A leap forward in #nanomedicine & #Polymer #Engineering. #Immunotherapy #DrugDelivery
March 9, 2026 at 1:47 PM
🎉 Max Schaaf defends his PhD at @mpip-mainz.mpg.de !
Developed smart #liposomes with polymer-based surfaces for targeted immune cell delivery: anti-CLEC9A antibodies, stable diABZI loading, in vivo efficacy.
A leap forward in #nanomedicine & #Polymer #Engineering. #Immunotherapy #DrugDelivery
Developed smart #liposomes with polymer-based surfaces for targeted immune cell delivery: anti-CLEC9A antibodies, stable diABZI loading, in vivo efficacy.
A leap forward in #nanomedicine & #Polymer #Engineering. #Immunotherapy #DrugDelivery
#PapersToRead Gasdermin-induced pyroptosis in macrophages leads to F-actin exposure for recognition by CLEC9A on dendritic cells
#NoTimeToDie! @inflammasomelab, Holley, Monteleone &co show @natimmunol.bsky.social that cells that die by #pyroptosis, or #necroptosis, are crowned with F-actin filopodia that makes them visible by dendritic cells via CLEC9A to initiate adaptive immunity! www.nature.com/articles/s41...
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells - Nature Immunology
Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate dendritic cells.
www.nature.com
December 7, 2024 at 6:10 PM
#PapersToRead Gasdermin-induced pyroptosis in macrophages leads to F-actin exposure for recognition by CLEC9A on dendritic cells
Now online - research briefing from @inflammasomelab.bsky.social Mercedes Monteleone on our recent @natimmunol.bsky.social publication on how pyroptotic cell corpses signal to dendritic cells from beyond the grave! #inflammasomes #pyroptosis #CellDeath #CLEC9A
Link: rdcu.be/d30EL
Link: rdcu.be/d30EL
December 19, 2024 at 3:53 AM
Now online - research briefing from @inflammasomelab.bsky.social Mercedes Monteleone on our recent @natimmunol.bsky.social publication on how pyroptotic cell corpses signal to dendritic cells from beyond the grave! #inflammasomes #pyroptosis #CellDeath #CLEC9A
Link: rdcu.be/d30EL
Link: rdcu.be/d30EL
In clever expts by Inflammasome Lab's Sabrina Burgener, DCs reacted to the calcium-sensitive filopodia of corpses by phosphorylating SYK via #CLEC9A. While #NINJ1-driven #CellExplosion was not needed for filopodial assembly (L; M. Monteleone), it was required to expose F-actin to #CLEC9A🤯. (11/13)
December 9, 2024 at 1:39 AM
In clever expts by Inflammasome Lab's Sabrina Burgener, DCs reacted to the calcium-sensitive filopodia of corpses by phosphorylating SYK via #CLEC9A. While #NINJ1-driven #CellExplosion was not needed for filopodial assembly (L; M. Monteleone), it was required to expose F-actin to #CLEC9A🤯. (11/13)
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming DCs
www.nature.com/articles/s41...
@natimmunol.bsky.social l @inflammasomelab.bsky.social
www.nature.com/articles/s41...
@natimmunol.bsky.social l @inflammasomelab.bsky.social
December 5, 2024 at 12:01 AM
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming DCs
www.nature.com/articles/s41...
@natimmunol.bsky.social l @inflammasomelab.bsky.social
www.nature.com/articles/s41...
@natimmunol.bsky.social l @inflammasomelab.bsky.social
#paperoftheweek
F-actin-rich filopodia crown pyroptotic corpses and bind to DNGR-1, to activate dendritic cells & drive immunity! 🦠
Huge congrats @inflammasomelab.bsky.social & all co-authors 👏🏻
@natimmunol.bsky.social
www.nature.com/articles/s41...
F-actin-rich filopodia crown pyroptotic corpses and bind to DNGR-1, to activate dendritic cells & drive immunity! 🦠
Huge congrats @inflammasomelab.bsky.social & all co-authors 👏🏻
@natimmunol.bsky.social
www.nature.com/articles/s41...
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells - Nature Immunology
Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate dendritic cells.
www.nature.com
December 8, 2024 at 11:45 AM
#paperoftheweek
F-actin-rich filopodia crown pyroptotic corpses and bind to DNGR-1, to activate dendritic cells & drive immunity! 🦠
Huge congrats @inflammasomelab.bsky.social & all co-authors 👏🏻
@natimmunol.bsky.social
www.nature.com/articles/s41...
F-actin-rich filopodia crown pyroptotic corpses and bind to DNGR-1, to activate dendritic cells & drive immunity! 🦠
Huge congrats @inflammasomelab.bsky.social & all co-authors 👏🏻
@natimmunol.bsky.social
www.nature.com/articles/s41...
DCs feed on pyroptotic cells corpses via the engagement F-actin-rich filopodia by CLEC9A. Great work by the group of Kate Schroder, showing how DCs sense cells dying by pyroptosis.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells - Nature Immunology
Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate dendritic cells.
www.nature.com
February 4, 2025 at 8:33 AM
DCs feed on pyroptotic cells corpses via the engagement F-actin-rich filopodia by CLEC9A. Great work by the group of Kate Schroder, showing how DCs sense cells dying by pyroptosis.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
one of the things I love about pyroptosis is that, as opposed to the canonical “oh well sorry to bother you” of apoptosis, pyroptosis is a dramatic “AVENGE ME!” declaration before decorating your corpse with your ejected bones www.nature.com/articles/s41...
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells - Nature Immunology
Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate dendritic cells.
www.nature.com
December 10, 2024 at 4:02 AM
one of the things I love about pyroptosis is that, as opposed to the canonical “oh well sorry to bother you” of apoptosis, pyroptosis is a dramatic “AVENGE ME!” declaration before decorating your corpse with your ejected bones www.nature.com/articles/s41...
📰Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells
doi.org/10.1038/s415...
doi.org/10.1038/s415...
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells - Nature Immunology
Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate dendritic cells.
doi.org
December 5, 2024 at 3:19 PM
📰Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells
doi.org/10.1038/s415...
doi.org/10.1038/s415...
What a beautiful mechanistic 🧪 insight into the collaboration between macrophages and dendritic cells, even in death. Congratulations @inflammasomelab.bsky.social and team! doi.org/10.1038/s415...
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells - Nature Immunology
Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate dendritic cells.
doi.org
December 6, 2024 at 2:41 AM
What a beautiful mechanistic 🧪 insight into the collaboration between macrophages and dendritic cells, even in death. Congratulations @inflammasomelab.bsky.social and team! doi.org/10.1038/s415...
2/11
𝗗𝗡𝗚𝗥-𝟭 is expressed by type 1 conventional dendritic cells, and recognises F-actin exposed by dead cells, promoting cross-presentation to CD8 T cells.
But from previous work, we knew DNGR-1–deficient mice can still reject transplantable tumours…So what was it doing during tumour development? 🤔
𝗗𝗡𝗚𝗥-𝟭 is expressed by type 1 conventional dendritic cells, and recognises F-actin exposed by dead cells, promoting cross-presentation to CD8 T cells.
But from previous work, we knew DNGR-1–deficient mice can still reject transplantable tumours…So what was it doing during tumour development? 🤔
Loss of secreted gelsolin enhances response to anticancer therapies
Type 1 conventional dendritic cells (cDC1) play a critical role in priming anticancer cytotoxic CD8+ T cells. DNGR-1 (a.k.a. CLEC9A) is a cDC1 receptor that binds to F-actin exposed on necrotic cancer...
jitc.bmj.com
January 2, 2026 at 4:57 PM
2/11
𝗗𝗡𝗚𝗥-𝟭 is expressed by type 1 conventional dendritic cells, and recognises F-actin exposed by dead cells, promoting cross-presentation to CD8 T cells.
But from previous work, we knew DNGR-1–deficient mice can still reject transplantable tumours…So what was it doing during tumour development? 🤔
𝗗𝗡𝗚𝗥-𝟭 is expressed by type 1 conventional dendritic cells, and recognises F-actin exposed by dead cells, promoting cross-presentation to CD8 T cells.
But from previous work, we knew DNGR-1–deficient mice can still reject transplantable tumours…So what was it doing during tumour development? 🤔
Why do #pyroptotic cells use their final minutes to make #filopodia? Well, #filopodia are rich stores of F-actin under the plasma membrane. Could #CellExplosion during #pyroptosis reveal filopodial F-actin to engage the corpse antigen sampling DC receptor, #CLEC9A/#DNGR1? (10/13) (credit: BioRender)
December 9, 2024 at 1:39 AM
Why do #pyroptotic cells use their final minutes to make #filopodia? Well, #filopodia are rich stores of F-actin under the plasma membrane. Could #CellExplosion during #pyroptosis reveal filopodial F-actin to engage the corpse antigen sampling DC receptor, #CLEC9A/#DNGR1? (10/13) (credit: BioRender)
RRIDs were included in this in Vaccines paper. RRIDs improve reproducibility in scientific research. #RRID #accelerateopenscience #ReproducibleResearch
A Precision-Engineered DC-Targeting mRNA-LNP Neoantigen Vaccine Elicits Stronger T Cell Responses and Exhibits Superior Tumor Control
Background/Objectives: Messenger RNA (mRNA) vaccine technology has shown great potential in the prevention of infectious diseases and treatment of cancers, but its full potential is limited by non-specific delivery mediated by the current lipid nanoparticle (LNP) platform. Methods: Here, we developed a dendritic cell (DC)-targeting LNP incorporated with an ultra-high-affinity CLEC9A-specific nanobody that facilitates enhanced DC uptake but reduced liver accumulation. We assessed the therapeutic efficacy of nanobody-functionalized lipid nanoparticles (Nb-LNPs) in a mouse Lewis lung carcinoma (LLC) model, alongside an evaluation of T cell-mediated immune responses and dendritic cell activation, facilitated by the delivery of mRNA-based neoantigen vaccines. Results: Compared with the use of an unfunctionalized LNP, personalized mRNA cancer vaccines encapsulated with this Nb-LNP demonstrated not only superior anti-tumor effects but also a favorable bio-safety profile in a mouse Lewis lung
doi.org
March 14, 2026 at 7:00 AM
RRIDs were included in this in Vaccines paper. RRIDs improve reproducibility in scientific research. #RRID #accelerateopenscience #ReproducibleResearch
15/
The paper also added experiments deleting MHC II from RORγt+ DCs or CLEC9A+ cDC1s. Again, this looks like reviewer driven expansion rather than something essential to the original study.
The paper also added experiments deleting MHC II from RORγt+ DCs or CLEC9A+ cDC1s. Again, this looks like reviewer driven expansion rather than something essential to the original study.
July 29, 2026 at 11:33 PM
15/
The paper also added experiments deleting MHC II from RORγt+ DCs or CLEC9A+ cDC1s. Again, this looks like reviewer driven expansion rather than something essential to the original study.
The paper also added experiments deleting MHC II from RORγt+ DCs or CLEC9A+ cDC1s. Again, this looks like reviewer driven expansion rather than something essential to the original study.
4/17 Screening in mouse embryonic fibroblasts revealed 2 key combos: PIP (PU.1, IRF4, PRDM1) activated Clec9a reporter & CD11b expression showing cDC2-like cell programming; SII (SPIB, IRF8, IKZF2) activated the Clec9a & hCD2 lymphoid reporters & BST2 expression- pDC-like cell
August 29, 2025 at 4:14 PM
4/17 Screening in mouse embryonic fibroblasts revealed 2 key combos: PIP (PU.1, IRF4, PRDM1) activated Clec9a reporter & CD11b expression showing cDC2-like cell programming; SII (SPIB, IRF8, IKZF2) activated the Clec9a & hCD2 lymphoid reporters & BST2 expression- pDC-like cell
4/17 Screening in mouse embryonic fibroblasts revealed 2 key combos: PIP (PU.1, IRF4, & PRDM1) activated Clec9a reporter & CD11b expression showing cDC2-like cell programming; SII (SPIB, IRF8, & IKZF2) activated Clec9a & hCD2 lymphoid reporters & BST2 expression- pDC-like cell
August 29, 2025 at 3:22 PM
4/17 Screening in mouse embryonic fibroblasts revealed 2 key combos: PIP (PU.1, IRF4, & PRDM1) activated Clec9a reporter & CD11b expression showing cDC2-like cell programming; SII (SPIB, IRF8, & IKZF2) activated Clec9a & hCD2 lymphoid reporters & BST2 expression- pDC-like cell