Various genes show distinct exits and entrances with age in the open- source dataset, the largest of its kind to date.

Senescent cells (SnCs) have been described to accumulate in osteoarthritis (OA) joint tissues in response to injury, thereby participating in OA development and progression. However, clinical therapeu

Omega fatty acids have long been recognized for their benefits: supporting cardiovascular health, reducing systemic inflammation, and promoting brain development and cognitive function.

CRISPR discovery and development processes involve two critical stages: gene editing followed by confirmation that the intended result has been achieved. Editing reagents must be introduced into precious, sensitive – often scarce – cells without compromising viability, a time-consuming and potentially error-prone process when performed manually. The subsequent analysis to confirm that editing has been successful often relies on lengthy, complex sequencing-based workflows that can take days, or even weeks, delaying future experiments. However, automation can be used to increase the speed and reproducibility of CRISPR editing workflows. We have collaborated with biotech companies DropGenie and QUiCKR, using INTEGRA liquid handling solutions to automate low input electroporation and rapid edit quantification, respectively, enabling laboratories to benefit from streamlined CRISPR processes, from primary cell engineering to data-driven optimization. Combining miniaturization and automation Miniaturization and automation offer advantages to CRISPR researchers. Miniaturization allows laboratories to perform many more experiments with a lot less starting material, while automation allows the workflow to be performed faster and at a lower cost, improving throughput and repeatability, and simultaneously removing the possibility of human errors. DropGenie, based in Montreal, Canada, is focused on miniaturization of cell and molecular biology workflows, to accelerate the pace of gene...

Read the full paper: HOPX regulates the development and lineage progression of human stem cell-derived outer radial glia-like cells

Despite promising development as emerging “off-the-shelf” therapeutics against cancer, natural killer (NK) cells still faced considerable challenges in the solid tumor microenvironment (TME), including poor penetrance and immuno-suppression. Here, we employ spatial and single-cell transcriptomics to reveal a role for Nur77 in NK cell-mediated immunity against hepatocellular carcinoma (HCC). Orthogonal analysis of human and mouse HCC tumors indicate that the expression of NR4A1, encoding Nur77, is associated with NK cell proliferation, activation of the immunostimulatory AP-1 gene regulons, and better disease-free survival in HCC. Conditional ablation of Nr4a1 in NK cells perturbs their homeostasis and accelerates tumor progression in multiple tumor models. Conversely, the agonistic activation of Nur77 in NK cells ex-vivo or in-vivo enhances their anti-tumor functions. Mechanistically, downstream functional assays confirm that Nur77 activation attenuates CD36 expression in NK cells and

We talk with Carolyn Bertozzi about her Nobel Prize-winning work in chemistry – and the impact it could

For scRNA-seq, we collected 16 human meninges samples from 13 donors across PCW 5–13. We collected the whole meninges that peeled off the brain (Extended Data Fig. 1a,b). A total of 156,726 high-quality cells were used for further analysis (Fig. 1a), and the mean unique molecular identifiers (UMIs) per sample were 8,842. As expected, anucleated erythropoietic cells showed the lowest gene and UMI counts (Extended Data Fig. 1c); excluding those cells, the mean UMI count was 10,249 (Supplementary Table 1). An analysis of the distribution of maternal and paternal SNP alleles21 (Methods) indicated that all cells were fetal except some erythrocytes (Extended Data Fig. 1d). We found that the data were well integrated across samples even in the absence of batch correction, which we therefore omitted to avoid suppressing true biological variation (Extended Data Fig. 1e,f). We generated a total of 245 clusters, annotated to four levels of detail: cell type (n = 115), subclass (n = 105), class (n = 45) and superclass (n = 8). We computed metadata such as cell cycle score, fetal age and enriched genes for each cluster (Supplementary Table 2). The largest superclass consisted of FOXC1+ fibroblasts, which made up half the dataset, in...

New research suggests the brain develops from two separate groups of early cells, one forming the forebrain and midbrain and the other the hindbrain, challenging a model of brain development that dates back to 1952.

New research suggests the brain develops from two separate groups of early cells, one forming the forebrain and midbrain and the other the hindbrain, challenging a model of brain development that dates back to 1952.

Glial cells are essential for proper nervous system development and function, but a high resolution view of glial development is lacking. This study analyses longitudinal single-cell RNA sequencing of...

Researchers at NYU Abu Dhabi have discovered that a single cancer-causing gene can trigger different responses in cells, with some helping to prevent tumors while others may contribute to their development.

Sofab Inks has been awarded a Phase I Small Business Innovation Research (SBIR) grant worth US$300,165 from the National Science Foundation. The award will fund development of liquid-processable tin…

Differently from other myeloid cells, microglia derive exclusively from precursors originating within the yolk sac and migrate to the CNS under development, without any contribution from fetal liver o...

The development of treatment resistance is one of the key challenges in modern cancer and antibiotic therapy. New therapeutic approaches aim to curb the spread of resistance through the targeted timing of treatment cycles.

MIT researchers developed a handheld device that can gently collect living cells from specific locations to test for ovarian and other cancers. The technique could be used to catch cancers earlier and...