📜 MultiGreen: A multiplexing architecture for GreenGate cloning
🧑🔬 Vincent J. Pennetti, Peter R. LaFayette, Wayne Allen Parrott
📔 PLOS ONE
🔗 dx.plos.org/10.1371/jour...
#️⃣ #PlantScience #Cloning #PlantSynthBio #SyntheticBiology #CloningSystems #GreenGate #PlantGoldenGate #PlantMolecularBiology
🧑🔬 Vincent J. Pennetti, Peter R. LaFayette, Wayne Allen Parrott
📔 PLOS ONE
🔗 dx.plos.org/10.1371/jour...
#️⃣ #PlantScience #Cloning #PlantSynthBio #SyntheticBiology #CloningSystems #GreenGate #PlantGoldenGate #PlantMolecularBiology
MultiGreen: A multiplexing architecture for GreenGate cloning
Genetic modification of plants fundamentally relies upon customized vector designs. The ever-increasing complexity of transgenic constructs has led to increased adoption of modular cloning systems for...
dx.plos.org
September 27, 2024 at 2:45 PM
📜 MultiGreen: A multiplexing architecture for GreenGate cloning
🧑🔬 Vincent J. Pennetti, Peter R. LaFayette, Wayne Allen Parrott
📔 PLOS ONE
🔗 dx.plos.org/10.1371/jour...
#️⃣ #PlantScience #Cloning #PlantSynthBio #SyntheticBiology #CloningSystems #GreenGate #PlantGoldenGate #PlantMolecularBiology
🧑🔬 Vincent J. Pennetti, Peter R. LaFayette, Wayne Allen Parrott
📔 PLOS ONE
🔗 dx.plos.org/10.1371/jour...
#️⃣ #PlantScience #Cloning #PlantSynthBio #SyntheticBiology #CloningSystems #GreenGate #PlantGoldenGate #PlantMolecularBiology
14/ Shout-out to Zhihao Lai who developed this awesome vector-building system as a SIDE PROJECT during his PhD! 🧪 #Cloning #CloningSystems #plasmids #StructuralBiology
#protein #science
bsky.app/profile/davi...
#protein #science
bsky.app/profile/davi...
Have you always wanted to build multi-gene baculovirus expression vectors without the pain?
We introduce MoClo Baculo:
+ No PCR
+ No primers
+ Swap tags/ORFs easily
+ No more sequencing gazillion intermediate plasmids
+ Bonus: yeast compatibility
🧵
www.biorxiv.org/content/10.1...
We introduce MoClo Baculo:
+ No PCR
+ No primers
+ Swap tags/ORFs easily
+ No more sequencing gazillion intermediate plasmids
+ Bonus: yeast compatibility
🧵
www.biorxiv.org/content/10.1...
Modular cloning of multigene vectors for the baculovirus system and yeast
Recombinant macromolecular complexes are often produced by the baculovirus system, using multigene expression vectors. Yet, the construction of baculovirus-compatible multigene expression vectors is c...
www.biorxiv.org
October 11, 2024 at 1:38 PM
14/ Shout-out to Zhihao Lai who developed this awesome vector-building system as a SIDE PROJECT during his PhD! 🧪 #Cloning #CloningSystems #plasmids #StructuralBiology
#protein #science
bsky.app/profile/davi...
#protein #science
bsky.app/profile/davi...