New pre-print: "Systematic comparison of colocalization methods using protein quantitative trait loci" led by @hwang_seongwon at www.biorxiv.org/content/10.1.... Which method does best? Find out!
Systematic comparison of colocalization methods using protein quantitative trait loci
Colocalization is frequently performed as a step to triage findings from genetic investigations linking molecular and disease data. However, the reliability and consistency of the various colocalizati...
www.biorxiv.org
November 8, 2025 at 3:40 PM
New pre-print: "Systematic comparison of colocalization methods using protein quantitative trait loci" led by @hwang_seongwon at www.biorxiv.org/content/10.1.... Which method does best? Find out!
Clever use of proteomic data to stress-test TWAS and QTL colocalization methods, revealing a high false sign rate. This hypothesis about high-LD and cross-tissue confounding is particularly interesting:
January 6, 2026 at 5:52 PM
Clever use of proteomic data to stress-test TWAS and QTL colocalization methods, revealing a high false sign rate. This hypothesis about high-LD and cross-tissue confounding is particularly interesting:
Excited to share this preprint from first author Jon Rosen, a postdoctoral fellow in the @klmohlke.bsky.social lab and my lab. We examine eQTL study sample size and how this affects signal discovery and rates of colocalization with GWAS.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Higher eQTL power reveals signals that boost GWAS colocalization
Expression quantitative trait locus (eQTL) studies in human cohorts typically detect at least one regulatory signal per gene, and have been proposed as a way to explain mechanisms of genetic liability...
www.biorxiv.org
August 18, 2025 at 12:18 PM
Excited to share this preprint from first author Jon Rosen, a postdoctoral fellow in the @klmohlke.bsky.social lab and my lab. We examine eQTL study sample size and how this affects signal discovery and rates of colocalization with GWAS.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
🧬 New today!
📄 Higher eQTL power reveals signals that boost GWAS colocalization
🧑🤝🧑 @mikelove.bsky.social @klmohlke.bsky.social & colleagues
📄 Higher eQTL power reveals signals that boost GWAS colocalization
🧑🤝🧑 @mikelove.bsky.social @klmohlke.bsky.social & colleagues
Higher eQTL power reveals signals that boost GWAS colocalization
Rosen et al. quantify how eQTL study sample size influences signal discovery and GWAS
colocalization. Integrating evidence from recent eQTL studies, they show that limited
statistical power influences...
www.cell.com
March 4, 2026 at 3:40 PM
🧬 New today!
📄 Higher eQTL power reveals signals that boost GWAS colocalization
🧑🤝🧑 @mikelove.bsky.social @klmohlke.bsky.social & colleagues
📄 Higher eQTL power reveals signals that boost GWAS colocalization
🧑🤝🧑 @mikelove.bsky.social @klmohlke.bsky.social & colleagues
🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!
January 28, 2026 at 4:20 PM
🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!
Good discussion & study-design recommendations in this new preprint by @mikelove.bsky.social @klmohlke.bsky.social & colleagues:
Higher eQTL power reveals signals that boost GWAS colocalization 🧪🧬
www.biorxiv.org/content/10.1...
Higher eQTL power reveals signals that boost GWAS colocalization 🧪🧬
www.biorxiv.org/content/10.1...
Higher eQTL power reveals signals that boost GWAS colocalization
Expression quantitative trait locus (eQTL) studies in human cohorts typically detect at least one regulatory signal per gene, and have been proposed as a way to explain mechanisms of genetic liability...
www.biorxiv.org
August 6, 2025 at 12:45 PM
Good discussion & study-design recommendations in this new preprint by @mikelove.bsky.social @klmohlke.bsky.social & colleagues:
Higher eQTL power reveals signals that boost GWAS colocalization 🧪🧬
www.biorxiv.org/content/10.1...
Higher eQTL power reveals signals that boost GWAS colocalization 🧪🧬
www.biorxiv.org/content/10.1...
Your numbers after stringent colocalization seem quite consistent with theirs (tagging @nikhilmilind.dev @jkpritch.bsky.social) . Colocalization is clearly needed, but it kills recall and that's a difficult thing to get around with standard sample sizes...
January 7, 2026 at 9:54 AM
Your numbers after stringent colocalization seem quite consistent with theirs (tagging @nikhilmilind.dev @jkpritch.bsky.social) . Colocalization is clearly needed, but it kills recall and that's a difficult thing to get around with standard sample sizes...
Martin Emons on SpatialFDA - searching for differential colocalization in spatial omics data
September 18, 2025 at 9:46 AM
Martin Emons on SpatialFDA - searching for differential colocalization in spatial omics data
Single-nucleus multimodal spatial transcriptomics reveals spatial colocalization of neoantigen-expressing tumor cells and cognate T cells - @danafarber.bsky.social @harvardmed.bsky.social @broadinstitute.org www.nature.com/articles/s41...
Single-nucleus multimodal spatial transcriptomics reveals spatial colocalization of neoantigen-expressing tumor cells and cognate T cells - Nature Biotechnology
Spatial relationships between tumor neoantigens and cognate T cells are studied.
www.nature.com
July 23, 2026 at 3:52 PM
Single-nucleus multimodal spatial transcriptomics reveals spatial colocalization of neoantigen-expressing tumor cells and cognate T cells - @danafarber.bsky.social @harvardmed.bsky.social @broadinstitute.org www.nature.com/articles/s41...
Preprint: Cortical microtubules act as a template to organize nano-scale patterning of exocytosis
www.biorxiv.org/content/10.1...
Image: Colocalization of TUA5 (green) and KEULE (magenta) in epidermal cells.
www.biorxiv.org/content/10.1...
Image: Colocalization of TUA5 (green) and KEULE (magenta) in epidermal cells.
December 7, 2024 at 4:30 PM
Preprint: Cortical microtubules act as a template to organize nano-scale patterning of exocytosis
www.biorxiv.org/content/10.1...
Image: Colocalization of TUA5 (green) and KEULE (magenta) in epidermal cells.
www.biorxiv.org/content/10.1...
Image: Colocalization of TUA5 (green) and KEULE (magenta) in epidermal cells.
Today @bethcimini.bsky.social and @damiandn.bsky.social lectured on image correction and colocalization. Key take-home: The computer can’t do magic; if you don’t collect photons at the microscope you can’t make them appear later.
April 11, 2026 at 5:30 PM
Today @bethcimini.bsky.social and @damiandn.bsky.social lectured on image correction and colocalization. Key take-home: The computer can’t do magic; if you don’t collect photons at the microscope you can’t make them appear later.
🔬Are you interested in applying colocalization analysis to your microscopy image data?
💡Check out my new video on colocalization metrics on the
Center for Open BioImage Analysis (COBA)
youtube channel!
www.youtube.com/watch?v=Ktx9...
💡Check out my new video on colocalization metrics on the
Center for Open BioImage Analysis (COBA)
youtube channel!
www.youtube.com/watch?v=Ktx9...
Exploring and Understanding Colocalization Metrics
YouTube video by COBA: Center for Open Bioimage Analysis
www.youtube.com
September 4, 2025 at 5:11 PM
🔬Are you interested in applying colocalization analysis to your microscopy image data?
💡Check out my new video on colocalization metrics on the
Center for Open BioImage Analysis (COBA)
youtube channel!
www.youtube.com/watch?v=Ktx9...
💡Check out my new video on colocalization metrics on the
Center for Open BioImage Analysis (COBA)
youtube channel!
www.youtube.com/watch?v=Ktx9...
#fluorescencefriday brings you a cleared AD mouse 🧠 stained for Abeta (green) and vasculature (magenta). Colocalization is white. #lightsheetmicroscopy
👀 for Abeta in blood vessels to test for CAA or micro bleeding. Courtesy of @shahrzadbahrampour.bsky.social Luke Smolders and Arash Salahinejad
👀 for Abeta in blood vessels to test for CAA or micro bleeding. Courtesy of @shahrzadbahrampour.bsky.social Luke Smolders and Arash Salahinejad
March 21, 2025 at 5:47 PM
#fluorescencefriday brings you a cleared AD mouse 🧠 stained for Abeta (green) and vasculature (magenta). Colocalization is white. #lightsheetmicroscopy
👀 for Abeta in blood vessels to test for CAA or micro bleeding. Courtesy of @shahrzadbahrampour.bsky.social Luke Smolders and Arash Salahinejad
👀 for Abeta in blood vessels to test for CAA or micro bleeding. Courtesy of @shahrzadbahrampour.bsky.social Luke Smolders and Arash Salahinejad
How a trick from flow cytometry can help colocalization visualization for microscopy forum.image.sc/t/cytofluoro...
October 31, 2025 at 10:32 AM
How a trick from flow cytometry can help colocalization visualization for microscopy forum.image.sc/t/cytofluoro...
Higher eQTL power reveals signals that boost GWAS colocalization www.biorxiv.org/content/10.1... 🧬🖥️🧪
August 15, 2025 at 6:30 PM
Higher eQTL power reveals signals that boost GWAS colocalization www.biorxiv.org/content/10.1... 🧬🖥️🧪
EASI-ORC is published!
It is an image analysis pipeline for the efficient analysis and segmentation of smFISH images for organelle-RNA colocalization measurements in yeast.
Developed by Shahar & Liav from the lab.
#RNASky #RNABiology 🧪🔬
www.nature.com/articles/s42...
It is an image analysis pipeline for the efficient analysis and segmentation of smFISH images for organelle-RNA colocalization measurements in yeast.
Developed by Shahar & Liav from the lab.
#RNASky #RNABiology 🧪🔬
www.nature.com/articles/s42...
EASI-ORC: A pipeline for the efficient analysis and segmentation of smFISH images for organelle-RNA colocalization measurements in yeast - Communications Biology
The EASI-ORC pipeline provides a robust toolset for the analysis of fluorescent microscopy images of yeast for mRNA-organelle colocalization. An accompanying step-by-step user guide allows for easy an...
www.nature.com
February 19, 2025 at 11:04 AM
EASI-ORC is published!
It is an image analysis pipeline for the efficient analysis and segmentation of smFISH images for organelle-RNA colocalization measurements in yeast.
Developed by Shahar & Liav from the lab.
#RNASky #RNABiology 🧪🔬
www.nature.com/articles/s42...
It is an image analysis pipeline for the efficient analysis and segmentation of smFISH images for organelle-RNA colocalization measurements in yeast.
Developed by Shahar & Liav from the lab.
#RNASky #RNABiology 🧪🔬
www.nature.com/articles/s42...
An excellent primer on co-localization analysis from Federico Gasparoli! youtu.be/Mv4M1HaYdBc @fedegasparoli.bsky.social
Introduction to Colocalization in Fluorescence Microscopy
YouTube video by Microcourses
youtu.be
November 4, 2024 at 10:13 PM
An excellent primer on co-localization analysis from Federico Gasparoli! youtu.be/Mv4M1HaYdBc @fedegasparoli.bsky.social
We updated our preprint, "Mapping spatially organized molecular and genetic signatures of schizophrenia across multiple scales in human prefrontal cortex" to now include spatial eQTL and genetic colocalization analyses: www.biorxiv.org/content/10.6...
May 22, 2026 at 1:21 AM
We updated our preprint, "Mapping spatially organized molecular and genetic signatures of schizophrenia across multiple scales in human prefrontal cortex" to now include spatial eQTL and genetic colocalization analyses: www.biorxiv.org/content/10.6...
Check out this new approach to colocalization analysis from @owfpuls.bsky.social along with members of the AIC and Advanced Bioimaging Unit teams, now out in @jcellsci.bsky.social!
journals.biologists.com/jcs/article/...
journals.biologists.com/jcs/article/...
🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!
January 28, 2026 at 5:53 PM
Check out this new approach to colocalization analysis from @owfpuls.bsky.social along with members of the AIC and Advanced Bioimaging Unit teams, now out in @jcellsci.bsky.social!
journals.biologists.com/jcs/article/...
journals.biologists.com/jcs/article/...
Colocalization isn't just "overlap"—it's potentially 4 distinct analyses or more.
Check out how to dissect colocalization analysis and get a glance on pitfalls and methods to report robust colocalization results.
Read newsletter now! bit.ly/46mYBL7
#Microscopy #BioImageAnalysis #Colocalization
Check out how to dissect colocalization analysis and get a glance on pitfalls and methods to report robust colocalization results.
Read newsletter now! bit.ly/46mYBL7
#Microscopy #BioImageAnalysis #Colocalization
November 5, 2025 at 9:10 AM
Colocalization isn't just "overlap"—it's potentially 4 distinct analyses or more.
Check out how to dissect colocalization analysis and get a glance on pitfalls and methods to report robust colocalization results.
Read newsletter now! bit.ly/46mYBL7
#Microscopy #BioImageAnalysis #Colocalization
Check out how to dissect colocalization analysis and get a glance on pitfalls and methods to report robust colocalization results.
Read newsletter now! bit.ly/46mYBL7
#Microscopy #BioImageAnalysis #Colocalization
Headed to WashU Genetics this week to talk about eQTL, GWAS, colocalization, and massively parallel reporter assays
April 10, 2024 at 12:13 AM
Headed to WashU Genetics this week to talk about eQTL, GWAS, colocalization, and massively parallel reporter assays
Higher eQTL power reveals signals that boost GWAS colocalization https://www.biorxiv.org/content/10.1101/2025.08.05.668745v1
August 6, 2025 at 6:32 AM
Higher eQTL power reveals signals that boost GWAS colocalization https://www.biorxiv.org/content/10.1101/2025.08.05.668745v1
Great talk today from Pete Castaldi at Channing, GWAS, colocalization, splicing, in COPD. Diving into mechanisms behind the ~80 known loci
cdnm.bwh.harvard.edu/peter-castal...
cdnm.bwh.harvard.edu/peter-castal...
Peter Castaldi, MD – Channing Division of Network Medicine
cdnm.bwh.harvard.edu
February 7, 2025 at 7:03 PM
Great talk today from Pete Castaldi at Channing, GWAS, colocalization, splicing, in COPD. Diving into mechanisms behind the ~80 known loci
cdnm.bwh.harvard.edu/peter-castal...
cdnm.bwh.harvard.edu/peter-castal...
Now: Dmitry Shungin: Comprehensive Proteomic Characterization of GLP1R Therapy: Leveraging Mendelian Randomization and Colocalization with Validation in Clinical Trials #ASHG25 🧪🧬🖥️
PheedLoop
meetings.ashg.org
October 14, 2025 at 9:25 PM
Now: Dmitry Shungin: Comprehensive Proteomic Characterization of GLP1R Therapy: Leveraging Mendelian Randomization and Colocalization with Validation in Clinical Trials #ASHG25 🧪🧬🖥️
9) Another interesting region is above the genes DARS2 and RABGAP1L. The signal also looks similar but the colocalization analysis wasn't confident about it. I suspect there might be multiple separate signals at play here.
September 5, 2026 at 7:51 AM
9) Another interesting region is above the genes DARS2 and RABGAP1L. The signal also looks similar but the colocalization analysis wasn't confident about it. I suspect there might be multiple separate signals at play here.