they're turning the pigs transgener
November 11, 2026 at 5:27 AM
they're turning the pigs transgener
When I cloned the GFP-tagged giant 480 kD isoform of ankyrin-G, I got literally one colony. #oneisallyouneed
November 7, 2026 at 5:50 PM
When I cloned the GFP-tagged giant 480 kD isoform of ankyrin-G, I got literally one colony. #oneisallyouneed
The implications are sometimes just mildly annoying: someone refusing to use a YFP-tagged construct or recloning it to a GFP, because their scope "only" has GFP filters; or delaying an experiment to install new filters for YFP when their existing GFP ones were good enough.
November 9, 2026 at 9:08 AM
The implications are sometimes just mildly annoying: someone refusing to use a YFP-tagged construct or recloning it to a GFP, because their scope "only" has GFP filters; or delaying an experiment to install new filters for YFP when their existing GFP ones were good enough.
This has practical consequences for experimental design and interpretation. The distinct names and pseudocoloring used for GFP and YFP makes people think that you need different filter sets for them, and ergo they won't show up in each other's channels. Not true.
November 9, 2026 at 9:08 AM
This has practical consequences for experimental design and interpretation. The distinct names and pseudocoloring used for GFP and YFP makes people think that you need different filter sets for them, and ergo they won't show up in each other's channels. Not true.
Da kommer GFP produkter i konflikt med det, uansett hvor bagatellmessig vi synes det er. Om GFP får lov er det fritt frem for andre å gjøre det samme og vi vet jo at det sitter store aktører som gjerne vil ta over det markedet. Så, litt klønete, av begge parter.
November 11, 2026 at 9:51 AM
Da kommer GFP produkter i konflikt med det, uansett hvor bagatellmessig vi synes det er. Om GFP får lov er det fritt frem for andre å gjøre det samme og vi vet jo at det sitter store aktører som gjerne vil ta over det markedet. Så, litt klønete, av begge parter.
The other confusion arises from the term red-shifting. This means simply shifting a spectrum toward longer wavelengths, in the direction of the red.
For example, Roger and Jim Remington's 1996 Science paper on GFP structure talks about red-shifting GFP to make what become YFP.
For example, Roger and Jim Remington's 1996 Science paper on GFP structure talks about red-shifting GFP to make what become YFP.
November 9, 2026 at 9:14 AM
The other confusion arises from the term red-shifting. This means simply shifting a spectrum toward longer wavelengths, in the direction of the red.
For example, Roger and Jim Remington's 1996 Science paper on GFP structure talks about red-shifting GFP to make what become YFP.
For example, Roger and Jim Remington's 1996 Science paper on GFP structure talks about red-shifting GFP to make what become YFP.
It's not the money that wins, it's the quality of the candidates. The GFP had a bunch of losers running for state-wide races. Sure, they were rich, but that doesn't work in the current economy, or when there is so much obvious corruption. Flaunting wealth only makes people revolt.
November 6, 2026 at 1:30 PM
It's not the money that wins, it's the quality of the candidates. The GFP had a bunch of losers running for state-wide races. Sure, they were rich, but that doesn't work in the current economy, or when there is so much obvious corruption. Flaunting wealth only makes people revolt.
Weronika E. Stachera, Jeffrey B. Woodruff et al. reveal that the E3 ligase TRIM37 recognizes a C-terminal domain, called IDR+ASH8, on the #centrosome scaffold protein Cep192. The IDR+ASH8 motif is sufficient to transform EB1 and GFP into TRIM37 substrates. rupress.org/jcb/article/...
#CellDivision
#CellDivision
November 10, 2026 at 5:01 PM
Weronika E. Stachera, Jeffrey B. Woodruff et al. reveal that the E3 ligase TRIM37 recognizes a C-terminal domain, called IDR+ASH8, on the #centrosome scaffold protein Cep192. The IDR+ASH8 motif is sufficient to transform EB1 and GFP into TRIM37 substrates. rupress.org/jcb/article/...
#CellDivision
#CellDivision
there is also the classic "GFP and RFP have 'green' and 'red' in it therefore..."
November 7, 2026 at 10:40 PM
there is also the classic "GFP and RFP have 'green' and 'red' in it therefore..."
Yes it depends on the definition. But to apply the generalized friendship paradox (GFP), the "colleagues" should be "your" colleagues rather than just defined by position/institutions. It's because GFP is a network phenomenon by definition.
November 5, 2026 at 2:43 AM
Yes it depends on the definition. But to apply the generalized friendship paradox (GFP), the "colleagues" should be "your" colleagues rather than just defined by position/institutions. It's because GFP is a network phenomenon by definition.
Thanks for the reply! YFP and GFP do look identical to me in that last image but I also often think two colors are the same when they aren’t 🤷♂️.
November 10, 2026 at 1:04 AM
Thanks for the reply! YFP and GFP do look identical to me in that last image but I also often think two colors are the same when they aren’t 🤷♂️.
Yes, but the GFP is still in control of the Senate until Jan 3rd. They're all nuts, and looking for revenge.
November 6, 2026 at 1:41 PM
Yes, but the GFP is still in control of the Senate until Jan 3rd. They're all nuts, and looking for revenge.
A bigger concern is someone attempting orthogonal YFP and GFP channels with overlapping filter sets (can be done but not with typical sets), and mistaking bleedthrough for colocalization. This seems very rare as it's not common to do this, and people tend to stick to previously established methods
November 9, 2026 at 9:08 AM
A bigger concern is someone attempting orthogonal YFP and GFP channels with overlapping filter sets (can be done but not with typical sets), and mistaking bleedthrough for colocalization. This seems very rare as it's not common to do this, and people tend to stick to previously established methods
Mir gefällt besonders, wie die Ausstellung verdeutlicht, dass der Begriff der „illegalen Migration“ zunächst in der lokalen Presse für Deutsche verwendet wurde, die in der Westzone als „illegal“ galten. Wie ironisch.
November 8, 2026 at 1:07 PM
Mir gefällt besonders, wie die Ausstellung verdeutlicht, dass der Begriff der „illegalen Migration“ zunächst in der lokalen Presse für Deutsche verwendet wurde, die in der Westzone als „illegal“ galten. Wie ironisch.
That awful 1960s ice blue. And that pink. Appalling combo. ANd click through for the reverse view... even worse.
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November 8, 2026 at 9:40 PM
That awful 1960s ice blue. And that pink. Appalling combo. ANd click through for the reverse view... even worse.
So MS NOW interviews GFP voters, and asks if they voted the same way again. "Well, he didn't do right by us, because of the tariffs and the Iran war. But, I voted GFP again anyway"!! So folks, don't make plans to celebrate. These people are really, REALLY DUMB! ...There's always hope, I guess.
November 4, 2026 at 12:32 AM
So MS NOW interviews GFP voters, and asks if they voted the same way again. "Well, he didn't do right by us, because of the tariffs and the Iran war. But, I voted GFP again anyway"!! So folks, don't make plans to celebrate. These people are really, REALLY DUMB! ...There's always hope, I guess.
With mNeonGreen, mClover3, and mGreenLantern which are the 3 brightest GFPs, the ideal GFP filter becomes even closer to the ideal YFP one, because the emissions of these 3 GFPs are in between EGFP and EYFP, as below.
(mNeonGreen and Clover curves are right on top of each other)
(mNeonGreen and Clover curves are right on top of each other)
November 9, 2026 at 9:08 AM
With mNeonGreen, mClover3, and mGreenLantern which are the 3 brightest GFPs, the ideal GFP filter becomes even closer to the ideal YFP one, because the emissions of these 3 GFPs are in between EGFP and EYFP, as below.
(mNeonGreen and Clover curves are right on top of each other)
(mNeonGreen and Clover curves are right on top of each other)
![Drafts
8d
straight women used to presume me a straight woman and be frankly very overfamiliar with me, which was incredibly confusingp
1mo
“if a fighter is your greatest experiment,
you pulled it by biting every question”
— #fridgle 42
https://fridgle.net/p/6L3
1mo
sorry for thirsting over the pope. I don't know what's wrong with me
3mo
they're turning the pigs transgener [journal figure of GFP pigs]](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:qgpgksazyjrdn4kckg7lcck5/bafkreidnvi7oclaydxjc3ibszeyihd6xl3o3jib6rurig6g45dey72rxce)
GFP? Goddamn Fucking Pinheads?
November 4, 2026 at 12:42 AM
GFP? Goddamn Fucking Pinheads?
Yes, one has to take the spectrum and multiply by your nervous system's sensitivity. But where most of the photons lie on the spectrum will tend to dominate.
In any case, you can look at the last image in the thread. Not sure if you can tell but YFP is just like GFP, and dsRed is not like mCherry
In any case, you can look at the last image in the thread. Not sure if you can tell but YFP is just like GFP, and dsRed is not like mCherry
November 10, 2026 at 12:58 AM
Yes, one has to take the spectrum and multiply by your nervous system's sensitivity. But where most of the photons lie on the spectrum will tend to dominate.
In any case, you can look at the last image in the thread. Not sure if you can tell but YFP is just like GFP, and dsRed is not like mCherry
In any case, you can look at the last image in the thread. Not sure if you can tell but YFP is just like GFP, and dsRed is not like mCherry
We even found that swapping in a synthetic nanobody-GFP adhesion system can partially restore branch orientation, hinting that a simple, sturdy physical connection can sometimes do the heavy lifting of preserving neural architecture.
November 11, 2026 at 2:16 PM
We even found that swapping in a synthetic nanobody-GFP adhesion system can partially restore branch orientation, hinting that a simple, sturdy physical connection can sometimes do the heavy lifting of preserving neural architecture.
Ask those countries club to take responsibility (i even forget that name. GFP? GFC? AHELAH LUPAA 🤣) And keep voicing. Let the world knw it, habibi 🍉 speak louder.
November 8, 2026 at 6:27 PM
Ask those countries club to take responsibility (i even forget that name. GFP? GFC? AHELAH LUPAA 🤣) And keep voicing. Let the world knw it, habibi 🍉 speak louder.
It’s not the GOP, it’s the GFP (the get FUCKED party).
November 4, 2026 at 5:26 AM
It’s not the GOP, it’s the GFP (the get FUCKED party).
The sperm-specific phospholipase C isoform PLCζ1 initiates fertilization Ca2+ oscillations in mammals, yet its loss does not eliminate #fertility. This study shows that PLCδ4 is a crucial backup oscillator supporting robust fertilization in mammals.
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
November 9, 2026 at 5:04 PM
The sperm-specific phospholipase C isoform PLCζ1 initiates fertilization Ca2+ oscillations in mammals, yet its loss does not eliminate #fertility. This study shows that PLCδ4 is a crucial backup oscillator supporting robust fertilization in mammals.
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq